Rh1DG120900

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0010284
Physical Location & Seq
Forward (+)
22103115 .. 22116882
13768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG120900.1

Sequence Viewer

Length: 2850 bp
ATGAAATCCTCAGTCTCGGCCATAACCCCTACCCCAACCCCCAACATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCCCCGCGACGACGACGACGACCTCCTCGCAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCAGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCCTGATTCTAAAGGCAAGCGAAGTGCAATCATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTCAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCGGCTGTAACATGGTTGGCATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATTTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAGGGGTATGTTAAGCGGATGAAGCCCAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCAACCGGAATCATAGCAATTCAACTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTCGGGGTTCTGTAGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGATATTATACCAGCAGCTATGTGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCAGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTCGTAAGCCAAAGTGGTACCGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCTCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGCAATTCATGGTTGA

Protein Analysis

949

Amino Acids

104.66

Weight (kDa)

7.27

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 126 - 567 7.3e-156 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 601 - 647 2.5e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 651 - 701 3.5e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 708 - 751 3.9e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 755 - 801 4e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 827 - 872 9.2e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 885 - 930 2.7e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1675
Acc36I ACCTGC 2 cut(s) 772, 1675
Acc65I GGTACC 2 cut(s) 1877, 2678
AccB1I GGYRCC 3 cut(s) 1877, 2678, 2806
AccBSI CCGCTC 2 cut(s) 109, 201
AccI GTMKAC 1 cut(s) 1986
AccII CGCG 2 cut(s) 222, 396
AclWI GGATC 4 cut(s) 573, 620, 1212, 1225
AcoI YGGCCR 2 cut(s) 18, 373
AcsI RAATTY 6 cut(s) 509, 604, 778, 1388, 1638, 2007
AcuI CTGAAG 5 cut(s) 783, 1342, 1558, 1801, 1951
AdeI CACNNNGTG 1 cut(s) 654
AfaI GTAC 6 cut(s) 184, 366, 1879, 1964, 2038, 2680
AfiI CCNNNNNNNGG 5 cut(s) 46, 286, 328, 944, 2334
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 1854
AjiI CACGTC 1 cut(s) 1382
AjnI CCWGG 2 cut(s) 922, 2221
AleI CACNNNNGTG 1 cut(s) 533
AloI GAACNNNNNNTCC 6 cut(s) 595, 627, 2502, 2534, 2643, 2675
Alw21I GWGCWC 3 cut(s) 321, 417, 1209
Alw26I GTCTC 5 cut(s) 19, 68, 519, 1372, 2724
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 4 cut(s) 573, 620, 1212, 1225
AlwNI CAGNNNCTG 2 cut(s) 929, 2033
Ama87I CYCGRG 1 cut(s) 2343
ApaLI GTGCAC 1 cut(s) 413
ApoI RAATTY 6 cut(s) 509, 604, 778, 1388, 1638, 2007
AseI ATTAAT 1 cut(s) 2786
Asp718I GGTACC 2 cut(s) 1877, 2678
AspA2I CCTAGG 1 cut(s) 2329
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 9 cut(s) 189, 216, 926, 1078, 2218, 2242, 2474, 2590, 2659
AsuHPI GGTGA 2 cut(s) 2237, 2651
AvaI CYCGRG 1 cut(s) 2343
AvaII GGWCC 6 cut(s) 926, 1078, 2218, 2242, 2590, 2659
AvrII CCTAGG 1 cut(s) 2329
BaeGI GKGCMC 2 cut(s) 382, 417
BaeI ACNNNNGTAYC 2 cut(s) 952, 985
BamHI GGATCC 1 cut(s) 1217
BanI GGYRCC 3 cut(s) 1877, 2678, 2806
BanII GRGCYC 3 cut(s) 321, 1209, 2329
BauI CACGAG 1 cut(s) 1995
BbsI GAAGAC 1 cut(s) 1423
Bbv12I GWGCWC 3 cut(s) 321, 417, 1209
BbvCI CCTCAGC 1 cut(s) 1551
BceAI ACGGC 2 cut(s) 360, 2472
BciT130I CCWGG 2 cut(s) 924, 2223
BciVI GTATCC 1 cut(s) 700
BclI TGATCA 1 cut(s) 1951
BcoDI GTCTC 5 cut(s) 19, 68, 519, 1372, 2724
BfaI CTAG 7 cut(s) 186, 483, 503, 1352, 1728, 2330, 2460
BfmI CTRYAG 5 cut(s) 630, 1340, 1440, 2352, 2583
BfoI RGCGCY 1 cut(s) 671
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 2 cut(s) 772, 1675
BfuI GTATCC 1 cut(s) 700
BglI GCCNNNNNGGC 1 cut(s) 347
BglII AGATCT 1 cut(s) 2052
BlnI CCTAGG 1 cut(s) 2329
Bme1390I CCNGG 2 cut(s) 924, 2223
Bme18I GGWCC 6 cut(s) 926, 1078, 2218, 2242, 2590, 2659
BmeT110I CYCGRG 1 cut(s) 2343
BmgBI CACGTC 1 cut(s) 1382
BmgT120I GGNCC 9 cut(s) 189, 216, 926, 1078, 2218, 2242, 2474, 2590, 2659
BmiI GGNNCC 9 cut(s) 191, 218, 1080, 1219, 1879, 2220, 2243, 2680, 2808
BmrFI CCNGG 2 cut(s) 924, 2223
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 8 cut(s) 328, 610, 1352, 1514, 1734, 1906, 2288, 2736
BmuI ACTGGG 1 cut(s) 999
BpiI GAAGAC 1 cut(s) 1423
BpmI CTGGAG 4 cut(s) 963, 1250, 1769, 2133
Bpu10I CCTNAGC 3 cut(s) 52, 561, 1551
BpuEI CTTGAG 2 cut(s) 1193, 2545
BsaBI GATNNNNATC 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 5 cut(s) 546, 964, 2221, 2329, 2477
BsaWI WCCGGW 2 cut(s) 59, 2195
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 985, 1015, 2420, 2450
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 286, 328, 944, 2334
Bse1I ACTGG 7 cut(s) 410, 946, 994, 1189, 1267, 2049, 2220
Bse3DI GCAATG 3 cut(s) 441, 687, 1926
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 2 cut(s) 924, 2223
BseDI CCNNGG 5 cut(s) 546, 964, 2221, 2329, 2477
BseGI GGATG 6 cut(s) 514, 865, 1174, 1848, 2279, 2641
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 5 cut(s) 46, 286, 328, 944, 2334
BseMI GCAATG 3 cut(s) 441, 687, 1926
BseMII CTCAG 9 cut(s) 24, 180, 306, 575, 1530, 1542, 2004, 2004, 2487
BseNI ACTGG 7 cut(s) 410, 946, 994, 1189, 1267, 2049, 2220
BseRI GAGGAG 3 cut(s) 166, 230, 960
BseSI GKGCMC 2 cut(s) 382, 417
BseX3I CGGCCG 1 cut(s) 373
BseYI CCCAGC 1 cut(s) 192
BsgI GTGCAG 4 cut(s) 87, 676, 2038, 2236
Bsh1236I CGCG 2 cut(s) 222, 396
Bsh1285I CGRYCG 1 cut(s) 376
BshNI GGYRCC 3 cut(s) 1877, 2678, 2806
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 3 cut(s) 321, 417, 1209
BsiHKCI CYCGRG 1 cut(s) 2343
BsiSI CCGG 3 cut(s) 60, 386, 2196
BslFI GGGAC 3 cut(s) 442, 1064, 2204
BslI CCNNNNNNNGG 5 cut(s) 46, 286, 328, 944, 2334
BsmAI GTCTC 5 cut(s) 19, 68, 519, 1372, 2724
BsmBI CGTCTC 1 cut(s) 1372
BsmFI GGGAC 3 cut(s) 442, 1064, 2204
BsmI GAATGC 4 cut(s) 423, 1194, 1296, 1346
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 2343
Bsp1286I GDGCHC 5 cut(s) 321, 382, 417, 1209, 2329
Bsp143I GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
Bsp68I TCGCGA 1 cut(s) 396
BspCNI CTCAG 9 cut(s) 23, 179, 305, 574, 1531, 1543, 2003, 2005, 2488
BspFNI CGCG 2 cut(s) 222, 396
BspLI GGNNCC 9 cut(s) 191, 218, 1080, 1219, 1879, 2220, 2243, 2680, 2808
BspMAI CTGCAG 3 cut(s) 634, 1344, 2587
BspMI ACCTGC 2 cut(s) 772, 1675
BspPI GGATC 4 cut(s) 573, 620, 1212, 1225
BspT107I GGYRCC 3 cut(s) 1877, 2678, 2806
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 68
BsrBI CCGCTC 2 cut(s) 109, 201
BsrDI GCAATG 3 cut(s) 441, 687, 1926
BsrI ACTGG 7 cut(s) 410, 946, 994, 1189, 1267, 2049, 2220
BssECI CCNNGG 5 cut(s) 546, 964, 2221, 2329, 2477
BssMI GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
BssSI CACGAG 1 cut(s) 1995
BssT1I CCWWGG 3 cut(s) 546, 964, 2329
Bst2BI CACGAG 1 cut(s) 1995
Bst2UI CCWGG 2 cut(s) 924, 2223
Bst4CI ACNGT 7 cut(s) 401, 712, 1307, 1984, 2140, 2683, 2718
Bst6I CTCTTC 3 cut(s) 79, 1518, 2545
BstAFI CTTAAG 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 907
BstC8I GCNNGC 5 cut(s) 247, 769, 1052, 1344, 2389
BstDSI CCRYGG 1 cut(s) 2477
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 6 cut(s) 514, 865, 1174, 1848, 2279, 2641
BstFNI CGCG 2 cut(s) 222, 396
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 7 cut(s) 581, 628, 1220, 1954, 2055, 2119, 2758
BstMAI GTCTC 5 cut(s) 19, 68, 519, 1372, 2724
BstMBI GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 629, 907, 1744, 1846
BstNI CCWGG 2 cut(s) 924, 2223
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 2 cut(s) 922, 2221
BstSFI CTRYAG 5 cut(s) 630, 1340, 1440, 2352, 2583
BstSLI GKGCMC 2 cut(s) 382, 417
BstUI CGCG 2 cut(s) 222, 396
BstV2I GAAGAC 1 cut(s) 1423
BstX2I RGATCY 2 cut(s) 1217, 2052
BstYI RGATCY 2 cut(s) 1217, 2052
BstZI CGGCCG 1 cut(s) 373
BsuI GTATCC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 2477
BtgZI GCGATG 1 cut(s) 2288
BtrI CACGTC 1 cut(s) 1382
BtsCI GGATG 6 cut(s) 514, 865, 1174, 1848, 2279, 2641
BtsI GCAGTG 2 cut(s) 528, 896
BtsIMutI CAGTG 9 cut(s) 417, 528, 584, 896, 1182, 1825, 1975, 2042, 2145
BtuMI TCGCGA 1 cut(s) 396
BveI ACCTGC 2 cut(s) 772, 1675
Cac8I GCNNGC 5 cut(s) 247, 769, 1052, 1344, 2389
CaiI CAGNNNCTG 2 cut(s) 929, 2033
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 9 cut(s) 189, 216, 926, 1078, 2218, 2242, 2474, 2590, 2659
CseI GACGC 1 cut(s) 265
Csp6I GTAC 6 cut(s) 183, 365, 1878, 1963, 2037, 2679
CviQI GTAC 6 cut(s) 183, 365, 1878, 1963, 2037, 2679
DpnI GATC 7 cut(s) 580, 627, 1219, 1953, 2054, 2118, 2757
DpnII GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
DraIII CACNNNGTG 1 cut(s) 654
EaeI YGGCCR 2 cut(s) 18, 373
EagI CGGCCG 1 cut(s) 373
Eam1104I CTCTTC 3 cut(s) 79, 1518, 2545
EarI CTCTTC 3 cut(s) 79, 1518, 2545
EciI GGCGGA 2 cut(s) 56, 125
Ecl136II GAGCTC 2 cut(s) 319, 1207
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 3 cut(s) 546, 964, 2329
Eco147I AGGCCT 1 cut(s) 1446
Eco24I GRGCYC 3 cut(s) 321, 1209, 2329
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 2707
Eco47I GGWCC 6 cut(s) 926, 1078, 2218, 2242, 2590, 2659
Eco52I CGGCCG 1 cut(s) 373
Eco53kI GAGCTC 2 cut(s) 319, 1207
Eco57I CTGAAG 5 cut(s) 783, 1342, 1558, 1801, 1951
Eco88I CYCGRG 1 cut(s) 2343
EcoICRI GAGCTC 2 cut(s) 319, 1207
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 4 cut(s) 189, 216, 926, 1078
EcoRII CCWGG 2 cut(s) 922, 2221
EcoRV GATATC 1 cut(s) 2707
EcoT14I CCWWGG 3 cut(s) 546, 964, 2329
EcoT22I ATGCAT 1 cut(s) 441
EcoT38I GRGCYC 3 cut(s) 321, 1209, 2329
ErhI CCWWGG 3 cut(s) 546, 964, 2329
Esp3I CGTCTC 1 cut(s) 1372
FaqI GGGAC 3 cut(s) 442, 1064, 2204
FauI CCCGC 3 cut(s) 128, 227, 2694
FbaI TGATCA 1 cut(s) 1951
FblI GTMKAC 1 cut(s) 1986
FokI GGATG 6 cut(s) 501, 872, 1181, 1855, 2266, 2648
FriOI GRGCYC 3 cut(s) 321, 1209, 2329
FspBI CTAG 7 cut(s) 186, 483, 503, 1352, 1728, 2330, 2460
GlaI GCGC 1 cut(s) 669
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 4 cut(s) 963, 1250, 1769, 2133
HaeII RGCGCY 1 cut(s) 671
HapII CCGG 3 cut(s) 60, 386, 2196
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 670
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 3 cut(s) 1310, 1769, 2193
HindII GTYRAC 3 cut(s) 1310, 1769, 2193
HindIII AAGCTT 1 cut(s) 1485
HpaII CCGG 3 cut(s) 60, 386, 2196
HphI GGTGA 2 cut(s) 2237, 2651
Hpy166II GTNNAC 6 cut(s) 415, 1310, 1769, 1912, 1987, 2193
Hpy188III TCNNGA 7 cut(s) 395, 459, 472, 563, 887, 1037, 2012
Hpy8I GTNNAC 6 cut(s) 415, 1310, 1769, 1912, 1987, 2193
Hpy99I CGWCG 5 cut(s) 227, 230, 233, 236, 401
HpyAV CCTTC 5 cut(s) 782, 1572, 1825, 2152, 2366
HpyCH4III ACNGT 7 cut(s) 401, 712, 1307, 1984, 2140, 2683, 2718
HpyCH4IV ACGT 4 cut(s) 390, 1000, 1381, 1856
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 629, 907, 1744, 1846
HpySE526I ACGT 4 cut(s) 390, 1000, 1381, 1856
HspAI GCGC 1 cut(s) 668
KpnI GGTACC 2 cut(s) 1881, 2682
Ksp22I TGATCA 1 cut(s) 1951
Kzo9I GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
LmnI GCTCC 4 cut(s) 201, 206, 273, 324
LweI GCATC 8 cut(s) 328, 610, 1352, 1514, 1734, 1906, 2288, 2736
MaeI CTAG 7 cut(s) 186, 483, 503, 1352, 1728, 2330, 2460
MaeII ACGT 4 cut(s) 390, 1000, 1381, 1856
MaeIII GTNAC 7 cut(s) 89, 644, 1175, 1283, 1730, 1940, 2570
MalI GATC 7 cut(s) 580, 627, 1219, 1953, 2054, 2118, 2757
MbiI CCGCTC 2 cut(s) 109, 201
MboI GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
MboII GAAGA 7 cut(s) 66, 789, 1126, 1428, 1505, 1664, 2532
MfeI CAATTG 3 cut(s) 615, 1500, 1704
MflI RGATCY 2 cut(s) 1217, 2052
MhlI GDGCHC 5 cut(s) 321, 382, 417, 1209, 2329
MlyI GAGTC 2 cut(s) 284, 2745
MmeI TCCRAC 5 cut(s) 174, 472, 962, 1245, 2596
Mph1103I ATGCAT 1 cut(s) 441
MseI TTAA 7 cut(s) 405, 1584, 1836, 1860, 2367, 2694, 2786
MslI CAYNNNNRTG 5 cut(s) 519, 533, 1187, 1947, 1953
MspA1I CMGCKG 2 cut(s) 635, 2033
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 3 cut(s) 60, 386, 2196
MspR9I CCNGG 2 cut(s) 924, 2223
MunI CAATTG 3 cut(s) 615, 1500, 1704
Mva1269I GAATGC 4 cut(s) 423, 1194, 1296, 1346
MvaI CCWGG 2 cut(s) 924, 2223
MvnI CGCG 2 cut(s) 222, 396
MwoI GCNNNNNNNGC 8 cut(s) 129, 135, 338, 347, 629, 907, 1744, 1846
NdeII GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
NlaIV GGNNCC 9 cut(s) 191, 218, 1080, 1219, 1879, 2220, 2243, 2680, 2808
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 1175, 1730, 1940
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 441
NspI RCATGY 1 cut(s) 922
OliI CACNNNNGTG 1 cut(s) 533
PaqCI CACCTGC 1 cut(s) 1675
PceI AGGCCT 1 cut(s) 1446
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 249, 303
PctI GAATGC 4 cut(s) 423, 1194, 1296, 1346
PleI GAGTC 2 cut(s) 284, 2745
PpsI GAGTC 2 cut(s) 284, 2745
PpuMI RGGWCCY 2 cut(s) 926, 1078
PshBI ATTAAT 1 cut(s) 2786
Psp124BI GAGCTC 2 cut(s) 321, 1209
Psp5II RGGWCCY 2 cut(s) 926, 1078
Psp6I CCWGG 2 cut(s) 922, 2221
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 922, 2221
PspN4I GGNNCC 9 cut(s) 191, 218, 1080, 1219, 1879, 2220, 2243, 2680, 2808
PspPI GGNCC 9 cut(s) 189, 216, 926, 1078, 2218, 2242, 2474, 2590, 2659
PspPPI RGGWCCY 2 cut(s) 926, 1078
PstI CTGCAG 3 cut(s) 634, 1344, 2587
PstNI CAGNNNCTG 2 cut(s) 929, 2033
PsuI RGATCY 2 cut(s) 1217, 2052
PvuII CAGCTG 2 cut(s) 635, 2033
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 6 cut(s) 184, 366, 1879, 1964, 2038, 2680
RsaNI GTAC 6 cut(s) 183, 365, 1878, 1963, 2037, 2679
RseI CAYNNNNRTG 5 cut(s) 519, 533, 1187, 1947, 1953
SacI GAGCTC 2 cut(s) 321, 1209
SaqAI TTAA 7 cut(s) 405, 1584, 1836, 1860, 2367, 2694, 2786
Sau3AI GATC 7 cut(s) 578, 625, 1217, 1951, 2052, 2116, 2755
Sau96I GGNCC 9 cut(s) 189, 216, 926, 1078, 2218, 2242, 2474, 2590, 2659
SchI GAGTC 2 cut(s) 284, 2745
ScrFI CCNGG 2 cut(s) 924, 2223
SduI GDGCHC 5 cut(s) 321, 382, 417, 1209, 2329
SfaNI GCATC 8 cut(s) 328, 610, 1352, 1514, 1734, 1906, 2288, 2736
SfcI CTRYAG 5 cut(s) 630, 1340, 1440, 2352, 2583
SinI GGWCC 6 cut(s) 926, 1078, 2218, 2242, 2590, 2659
SmiMI CAYNNNNRTG 5 cut(s) 519, 533, 1187, 1947, 1953
SmlI CTYRAG 3 cut(s) 404, 1208, 2524
SmoI CTYRAG 3 cut(s) 404, 1208, 2524
SseBI AGGCCT 1 cut(s) 1446
SspI AATATT 1 cut(s) 1560
SspMI CTAG 7 cut(s) 186, 483, 503, 1352, 1728, 2330, 2460
SstI GAGCTC 2 cut(s) 321, 1209
StuI AGGCCT 1 cut(s) 1446
StyD4I CCNGG 2 cut(s) 922, 2221
StyI CCWWGG 3 cut(s) 546, 964, 2329
TaaI ACNGT 7 cut(s) 401, 712, 1307, 1984, 2140, 2683, 2718
TaiI ACGT 4 cut(s) 393, 1003, 1384, 1859
TaqI TCGA 5 cut(s) 985, 1742, 1873, 2323, 2754
TaqII GACCGA 2 cut(s) 689, 2607
TatI WGTACW 2 cut(s) 364, 1962
TauI GCSGC 4 cut(s) 132, 252, 423, 2025
Tru1I TTAA 7 cut(s) 405, 1584, 1836, 1860, 2367, 2694, 2786
Tru9I TTAA 7 cut(s) 405, 1584, 1836, 1860, 2367, 2694, 2786
TscAI CASTG 9 cut(s) 417, 535, 591, 903, 1189, 1825, 1975, 2049, 2145
TseFI GTSAC 3 cut(s) 1175, 1730, 1940
Tsp45I GTSAC 3 cut(s) 1175, 1730, 1940
TspGWI ACGGA 4 cut(s) 77, 146, 2234, 2304
TspRI CASTG 9 cut(s) 417, 535, 591, 903, 1189, 1825, 1975, 2049, 2145
Vha464I CTTAAG 1 cut(s) 404
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 6 cut(s) 926, 1078, 2218, 2242, 2590, 2659
VspI ATTAAT 1 cut(s) 2786
XagI CCTNNNNNAGG 1 cut(s) 284
XapI RAATTY 6 cut(s) 509, 604, 778, 1388, 1638, 2007
XceI RCATGY 1 cut(s) 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 2412
XmaJI CCTAGG 1 cut(s) 2329
XmiI GTMKAC 1 cut(s) 1986
XspI CTAG 7 cut(s) 186, 483, 503, 1352, 1728, 2330, 2460
Zsp2I ATGCAT 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.