Rh1CG112100

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0010284
Physical Location & Seq
Forward (+)
22624082 .. 22637791
13710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG112100.1

Sequence Viewer

Length: 2805 bp
ATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCCCCGCGACGACGACGACGACCTCCTCGCAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCAGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCCTGATTCTAAAGGCAAGCGAAGTGCAATCATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTCAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGCATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATTTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAGGGGTATGTTAAGCGGATGAAGCCCAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCAACCGGAATCATAGCAATTCAACTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTCGGGGTTCTGTAGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGATATTATACCAGCAGCTATGTGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCAGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTCGTAAGCCAAAGTGGTACCGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCTCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGCAATTCATGGTTGA

Protein Analysis

934

Amino Acids

103.18

Weight (kDa)

7.01

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 111 - 552 3.8e-156 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 586 - 632 2.4e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 636 - 686 3.4e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 693 - 736 3.8e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 740 - 786 3.9e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 812 - 857 9.1e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 870 - 915 2.6e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1630
Acc36I ACCTGC 2 cut(s) 727, 1630
Acc65I GGTACC 2 cut(s) 1832, 2633
AccB1I GGYRCC 3 cut(s) 1832, 2633, 2761
AccBSI CCGCTC 2 cut(s) 64, 156
AccI GTMKAC 1 cut(s) 1941
AccII CGCG 2 cut(s) 177, 351
AclWI GGATC 4 cut(s) 528, 575, 1167, 1180
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 6 cut(s) 464, 559, 733, 1343, 1593, 1962
AcuI CTGAAG 6 cut(s) 738, 1216, 1297, 1513, 1756, 1906
AdeI CACNNNGTG 1 cut(s) 609
AfaI GTAC 6 cut(s) 139, 321, 1834, 1919, 1993, 2635
AfiI CCNNNNNNNGG 4 cut(s) 241, 283, 899, 2289
AflII CTTAAG 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 1809
AjiI CACGTC 1 cut(s) 1337
AjnI CCWGG 2 cut(s) 877, 2176
AleI CACNNNNGTG 1 cut(s) 488
AloI GAACNNNNNNTCC 6 cut(s) 550, 582, 2457, 2489, 2598, 2630
Alw21I GWGCWC 3 cut(s) 276, 372, 1164
Alw26I GTCTC 4 cut(s) 23, 474, 1327, 2679
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 4 cut(s) 528, 575, 1167, 1180
AlwNI CAGNNNCTG 2 cut(s) 884, 1988
Ama87I CYCGRG 1 cut(s) 2298
ApaLI GTGCAC 1 cut(s) 368
ApoI RAATTY 6 cut(s) 464, 559, 733, 1343, 1593, 1962
AseI ATTAAT 1 cut(s) 2741
Asp718I GGTACC 2 cut(s) 1832, 2633
AspA2I CCTAGG 1 cut(s) 2284
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 9 cut(s) 144, 171, 881, 1033, 2173, 2197, 2429, 2545, 2614
AsuHPI GGTGA 2 cut(s) 2192, 2606
AvaI CYCGRG 1 cut(s) 2298
AvaII GGWCC 6 cut(s) 881, 1033, 2173, 2197, 2545, 2614
AvrII CCTAGG 1 cut(s) 2284
BaeGI GKGCMC 2 cut(s) 337, 372
BaeI ACNNNNGTAYC 2 cut(s) 907, 940
BamHI GGATCC 1 cut(s) 1172
BanI GGYRCC 3 cut(s) 1832, 2633, 2761
BanII GRGCYC 3 cut(s) 276, 1164, 2284
BauI CACGAG 1 cut(s) 1950
BbsI GAAGAC 1 cut(s) 1378
Bbv12I GWGCWC 3 cut(s) 276, 372, 1164
BbvCI CCTCAGC 1 cut(s) 1506
BceAI ACGGC 2 cut(s) 315, 2427
BciT130I CCWGG 2 cut(s) 879, 2178
BciVI GTATCC 1 cut(s) 655
BclI TGATCA 1 cut(s) 1906
BcoDI GTCTC 4 cut(s) 23, 474, 1327, 2679
BfaI CTAG 7 cut(s) 141, 438, 458, 1307, 1683, 2285, 2415
BfmI CTRYAG 5 cut(s) 585, 1295, 1395, 2307, 2538
BfoI RGCGCY 1 cut(s) 626
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 2 cut(s) 727, 1630
BfuI GTATCC 1 cut(s) 655
BglI GCCNNNNNGGC 1 cut(s) 302
BglII AGATCT 1 cut(s) 2007
BlnI CCTAGG 1 cut(s) 2284
Bme1390I CCNGG 2 cut(s) 879, 2178
Bme18I GGWCC 6 cut(s) 881, 1033, 2173, 2197, 2545, 2614
BmeT110I CYCGRG 1 cut(s) 2298
BmgBI CACGTC 1 cut(s) 1337
BmgT120I GGNCC 9 cut(s) 144, 171, 881, 1033, 2173, 2197, 2429, 2545, 2614
BmiI GGNNCC 9 cut(s) 146, 173, 1035, 1174, 1834, 2175, 2198, 2635, 2763
BmrFI CCNGG 2 cut(s) 879, 2178
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 8 cut(s) 283, 565, 1307, 1469, 1689, 1861, 2243, 2691
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 1 cut(s) 1378
BpmI CTGGAG 4 cut(s) 918, 1205, 1724, 2088
Bpu10I CCTNAGC 3 cut(s) 7, 516, 1506
BpuEI CTTGAG 2 cut(s) 1148, 2500
BsaBI GATNNNNATC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 501, 919, 2176, 2284, 2432
BsaWI WCCGGW 2 cut(s) 14, 2150
BsaXI ACNNNNNCTCC 6 cut(s) 86, 116, 940, 970, 2375, 2405
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 283, 899, 2289
Bse1I ACTGG 7 cut(s) 365, 901, 949, 1144, 1222, 2004, 2175
Bse3DI GCAATG 3 cut(s) 396, 642, 1881
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 2 cut(s) 879, 2178
BseDI CCNNGG 5 cut(s) 501, 919, 2176, 2284, 2432
BseGI GGATG 6 cut(s) 469, 820, 1129, 1803, 2234, 2596
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 4 cut(s) 241, 283, 899, 2289
BseMI GCAATG 3 cut(s) 396, 642, 1881
BseMII CTCAG 8 cut(s) 135, 261, 530, 1485, 1497, 1959, 1959, 2442
BseNI ACTGG 7 cut(s) 365, 901, 949, 1144, 1222, 2004, 2175
BseRI GAGGAG 3 cut(s) 121, 185, 915
BseSI GKGCMC 2 cut(s) 337, 372
BseX3I CGGCCG 1 cut(s) 328
BseYI CCCAGC 1 cut(s) 147
BsgI GTGCAG 4 cut(s) 42, 631, 1993, 2191
Bsh1236I CGCG 2 cut(s) 177, 351
Bsh1285I CGRYCG 1 cut(s) 331
BshNI GGYRCC 3 cut(s) 1832, 2633, 2761
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 3 cut(s) 276, 372, 1164
BsiHKCI CYCGRG 1 cut(s) 2298
BsiSI CCGG 3 cut(s) 15, 341, 2151
BslFI GGGAC 3 cut(s) 397, 1019, 2159
BslI CCNNNNNNNGG 4 cut(s) 241, 283, 899, 2289
BsmAI GTCTC 4 cut(s) 23, 474, 1327, 2679
BsmBI CGTCTC 1 cut(s) 1327
BsmFI GGGAC 3 cut(s) 397, 1019, 2159
BsmI GAATGC 4 cut(s) 378, 1149, 1251, 1301
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 1 cut(s) 2298
Bsp1286I GDGCHC 5 cut(s) 276, 337, 372, 1164, 2284
Bsp143I GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
Bsp68I TCGCGA 1 cut(s) 351
BspCNI CTCAG 8 cut(s) 134, 260, 529, 1486, 1498, 1958, 1960, 2443
BspFNI CGCG 2 cut(s) 177, 351
BspLI GGNNCC 9 cut(s) 146, 173, 1035, 1174, 1834, 2175, 2198, 2635, 2763
BspMAI CTGCAG 3 cut(s) 589, 1299, 2542
BspMI ACCTGC 2 cut(s) 727, 1630
BspPI GGATC 4 cut(s) 528, 575, 1167, 1180
BspT107I GGYRCC 3 cut(s) 1832, 2633, 2761
BspTI CTTAAG 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 23
BsrBI CCGCTC 2 cut(s) 64, 156
BsrDI GCAATG 3 cut(s) 396, 642, 1881
BsrI ACTGG 7 cut(s) 365, 901, 949, 1144, 1222, 2004, 2175
BssECI CCNNGG 5 cut(s) 501, 919, 2176, 2284, 2432
BssMI GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
BssSI CACGAG 1 cut(s) 1950
BssT1I CCWWGG 3 cut(s) 501, 919, 2284
Bst2BI CACGAG 1 cut(s) 1950
Bst2UI CCWGG 2 cut(s) 879, 2178
Bst4CI ACNGT 7 cut(s) 356, 667, 1262, 1939, 2095, 2638, 2673
Bst6I CTCTTC 3 cut(s) 34, 1473, 2500
BstAFI CTTAAG 1 cut(s) 359
BstAPI GCANNNNNTGC 1 cut(s) 862
BstC8I GCNNGC 5 cut(s) 202, 724, 1007, 1299, 2344
BstDSI CCRYGG 1 cut(s) 2432
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 6 cut(s) 469, 820, 1129, 1803, 2234, 2596
BstFNI CGCG 2 cut(s) 177, 351
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 7 cut(s) 536, 583, 1175, 1909, 2010, 2074, 2713
BstMAI GTCTC 4 cut(s) 23, 474, 1327, 2679
BstMBI GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 8 cut(s) 84, 90, 293, 302, 584, 862, 1699, 1801
BstNI CCWGG 2 cut(s) 879, 2178
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 2 cut(s) 877, 2176
BstSFI CTRYAG 5 cut(s) 585, 1295, 1395, 2307, 2538
BstSLI GKGCMC 2 cut(s) 337, 372
BstUI CGCG 2 cut(s) 177, 351
BstV2I GAAGAC 1 cut(s) 1378
BstX2I RGATCY 2 cut(s) 1172, 2007
BstYI RGATCY 2 cut(s) 1172, 2007
BstZI CGGCCG 1 cut(s) 328
BsuI GTATCC 1 cut(s) 655
BtgI CCRYGG 1 cut(s) 2432
BtgZI GCGATG 1 cut(s) 2243
BtrI CACGTC 1 cut(s) 1337
BtsCI GGATG 6 cut(s) 469, 820, 1129, 1803, 2234, 2596
BtsI GCAGTG 2 cut(s) 483, 851
BtsIMutI CAGTG 9 cut(s) 372, 483, 539, 851, 1137, 1780, 1930, 1997, 2100
BtuMI TCGCGA 1 cut(s) 351
BveI ACCTGC 2 cut(s) 727, 1630
Cac8I GCNNGC 5 cut(s) 202, 724, 1007, 1299, 2344
CaiI CAGNNNCTG 2 cut(s) 884, 1988
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 9 cut(s) 144, 171, 881, 1033, 2173, 2197, 2429, 2545, 2614
CseI GACGC 1 cut(s) 220
Csp6I GTAC 6 cut(s) 138, 320, 1833, 1918, 1992, 2634
CviQI GTAC 6 cut(s) 138, 320, 1833, 1918, 1992, 2634
DpnI GATC 7 cut(s) 535, 582, 1174, 1908, 2009, 2073, 2712
DpnII GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
DraIII CACNNNGTG 1 cut(s) 609
EaeI YGGCCR 1 cut(s) 328
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 3 cut(s) 34, 1473, 2500
EarI CTCTTC 3 cut(s) 34, 1473, 2500
EciI GGCGGA 2 cut(s) 11, 80
Ecl136II GAGCTC 2 cut(s) 274, 1162
EclXI CGGCCG 1 cut(s) 328
Eco130I CCWWGG 3 cut(s) 501, 919, 2284
Eco147I AGGCCT 1 cut(s) 1401
Eco24I GRGCYC 3 cut(s) 276, 1164, 2284
Eco31I GGTCTC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 2662
Eco47I GGWCC 6 cut(s) 881, 1033, 2173, 2197, 2545, 2614
Eco52I CGGCCG 1 cut(s) 328
Eco53kI GAGCTC 2 cut(s) 274, 1162
Eco57I CTGAAG 6 cut(s) 738, 1216, 1297, 1513, 1756, 1906
Eco88I CYCGRG 1 cut(s) 2298
EcoICRI GAGCTC 2 cut(s) 274, 1162
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 4 cut(s) 144, 171, 881, 1033
EcoRII CCWGG 2 cut(s) 877, 2176
EcoRV GATATC 1 cut(s) 2662
EcoT14I CCWWGG 3 cut(s) 501, 919, 2284
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 3 cut(s) 276, 1164, 2284
ErhI CCWWGG 3 cut(s) 501, 919, 2284
Esp3I CGTCTC 1 cut(s) 1327
FaqI GGGAC 3 cut(s) 397, 1019, 2159
FauI CCCGC 3 cut(s) 83, 182, 2649
FbaI TGATCA 1 cut(s) 1906
FblI GTMKAC 1 cut(s) 1941
FokI GGATG 6 cut(s) 456, 827, 1136, 1810, 2221, 2603
FriOI GRGCYC 3 cut(s) 276, 1164, 2284
FspBI CTAG 7 cut(s) 141, 438, 458, 1307, 1683, 2285, 2415
GlaI GCGC 1 cut(s) 624
GsaI CCCAGC 1 cut(s) 151
GsuI CTGGAG 4 cut(s) 918, 1205, 1724, 2088
HaeII RGCGCY 1 cut(s) 626
HapII CCGG 3 cut(s) 15, 341, 2151
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 625
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 3 cut(s) 1265, 1724, 2148
HindII GTYRAC 3 cut(s) 1265, 1724, 2148
HindIII AAGCTT 1 cut(s) 1440
HpaII CCGG 3 cut(s) 15, 341, 2151
HphI GGTGA 2 cut(s) 2192, 2606
Hpy166II GTNNAC 6 cut(s) 370, 1265, 1724, 1867, 1942, 2148
Hpy188III TCNNGA 7 cut(s) 350, 414, 427, 518, 842, 992, 1967
Hpy8I GTNNAC 6 cut(s) 370, 1265, 1724, 1867, 1942, 2148
Hpy99I CGWCG 5 cut(s) 182, 185, 188, 191, 356
HpyAV CCTTC 5 cut(s) 737, 1527, 1780, 2107, 2321
HpyCH4III ACNGT 7 cut(s) 356, 667, 1262, 1939, 2095, 2638, 2673
HpyCH4IV ACGT 4 cut(s) 345, 955, 1336, 1811
HpyF10VI GCNNNNNNNGC 8 cut(s) 84, 90, 293, 302, 584, 862, 1699, 1801
HpySE526I ACGT 4 cut(s) 345, 955, 1336, 1811
HspAI GCGC 1 cut(s) 623
KpnI GGTACC 2 cut(s) 1836, 2637
Ksp22I TGATCA 1 cut(s) 1906
Kzo9I GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
LmnI GCTCC 4 cut(s) 156, 161, 228, 279
LweI GCATC 8 cut(s) 283, 565, 1307, 1469, 1689, 1861, 2243, 2691
MaeI CTAG 7 cut(s) 141, 438, 458, 1307, 1683, 2285, 2415
MaeII ACGT 4 cut(s) 345, 955, 1336, 1811
MaeIII GTNAC 7 cut(s) 44, 599, 1130, 1238, 1685, 1895, 2525
MalI GATC 7 cut(s) 535, 582, 1174, 1908, 2009, 2073, 2712
MbiI CCGCTC 2 cut(s) 64, 156
MboI GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
MboII GAAGA 7 cut(s) 21, 744, 1081, 1383, 1460, 1619, 2487
MfeI CAATTG 3 cut(s) 570, 1455, 1659
MflI RGATCY 2 cut(s) 1172, 2007
MhlI GDGCHC 5 cut(s) 276, 337, 372, 1164, 2284
MlyI GAGTC 2 cut(s) 239, 2700
MmeI TCCRAC 5 cut(s) 129, 427, 917, 1200, 2551
Mph1103I ATGCAT 1 cut(s) 396
MseI TTAA 7 cut(s) 360, 1539, 1791, 1815, 2322, 2649, 2741
MslI CAYNNNNRTG 5 cut(s) 474, 488, 1142, 1902, 1908
MspA1I CMGCKG 3 cut(s) 590, 1236, 1988
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 3 cut(s) 15, 341, 2151
MspR9I CCNGG 2 cut(s) 879, 2178
MunI CAATTG 3 cut(s) 570, 1455, 1659
Mva1269I GAATGC 4 cut(s) 378, 1149, 1251, 1301
MvaI CCWGG 2 cut(s) 879, 2178
MvnI CGCG 2 cut(s) 177, 351
MwoI GCNNNNNNNGC 8 cut(s) 84, 90, 293, 302, 584, 862, 1699, 1801
NdeII GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
NlaIV GGNNCC 9 cut(s) 146, 173, 1035, 1174, 1834, 2175, 2198, 2635, 2763
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 3 cut(s) 1130, 1685, 1895
NruI TCGCGA 1 cut(s) 351
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 1 cut(s) 877
OliI CACNNNNGTG 1 cut(s) 488
PaqCI CACCTGC 1 cut(s) 1630
PceI AGGCCT 1 cut(s) 1401
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 204, 258
PctI GAATGC 4 cut(s) 378, 1149, 1251, 1301
PleI GAGTC 2 cut(s) 239, 2700
PpsI GAGTC 2 cut(s) 239, 2700
PpuMI RGGWCCY 2 cut(s) 881, 1033
PshBI ATTAAT 1 cut(s) 2741
Psp124BI GAGCTC 2 cut(s) 276, 1164
Psp5II RGGWCCY 2 cut(s) 881, 1033
Psp6I CCWGG 2 cut(s) 877, 2176
PspFI CCCAGC 1 cut(s) 147
PspGI CCWGG 2 cut(s) 877, 2176
PspN4I GGNNCC 9 cut(s) 146, 173, 1035, 1174, 1834, 2175, 2198, 2635, 2763
PspPI GGNCC 9 cut(s) 144, 171, 881, 1033, 2173, 2197, 2429, 2545, 2614
PspPPI RGGWCCY 2 cut(s) 881, 1033
PstI CTGCAG 3 cut(s) 589, 1299, 2542
PstNI CAGNNNCTG 2 cut(s) 884, 1988
PsuI RGATCY 2 cut(s) 1172, 2007
PvuII CAGCTG 3 cut(s) 590, 1236, 1988
RruI TCGCGA 1 cut(s) 351
RsaI GTAC 6 cut(s) 139, 321, 1834, 1919, 1993, 2635
RsaNI GTAC 6 cut(s) 138, 320, 1833, 1918, 1992, 2634
RseI CAYNNNNRTG 5 cut(s) 474, 488, 1142, 1902, 1908
SacI GAGCTC 2 cut(s) 276, 1164
SaqAI TTAA 7 cut(s) 360, 1539, 1791, 1815, 2322, 2649, 2741
Sau3AI GATC 7 cut(s) 533, 580, 1172, 1906, 2007, 2071, 2710
Sau96I GGNCC 9 cut(s) 144, 171, 881, 1033, 2173, 2197, 2429, 2545, 2614
SchI GAGTC 2 cut(s) 239, 2700
ScrFI CCNGG 2 cut(s) 879, 2178
SduI GDGCHC 5 cut(s) 276, 337, 372, 1164, 2284
SfaNI GCATC 8 cut(s) 283, 565, 1307, 1469, 1689, 1861, 2243, 2691
SfcI CTRYAG 5 cut(s) 585, 1295, 1395, 2307, 2538
SinI GGWCC 6 cut(s) 881, 1033, 2173, 2197, 2545, 2614
SmiMI CAYNNNNRTG 5 cut(s) 474, 488, 1142, 1902, 1908
SmlI CTYRAG 3 cut(s) 359, 1163, 2479
SmoI CTYRAG 3 cut(s) 359, 1163, 2479
SseBI AGGCCT 1 cut(s) 1401
SspI AATATT 1 cut(s) 1515
SspMI CTAG 7 cut(s) 141, 438, 458, 1307, 1683, 2285, 2415
SstI GAGCTC 2 cut(s) 276, 1164
StuI AGGCCT 1 cut(s) 1401
StyD4I CCNGG 2 cut(s) 877, 2176
StyI CCWWGG 3 cut(s) 501, 919, 2284
TaaI ACNGT 7 cut(s) 356, 667, 1262, 1939, 2095, 2638, 2673
TaiI ACGT 4 cut(s) 348, 958, 1339, 1814
TaqI TCGA 5 cut(s) 940, 1697, 1828, 2278, 2709
TaqII GACCGA 2 cut(s) 644, 2562
TatI WGTACW 2 cut(s) 319, 1917
TauI GCSGC 4 cut(s) 87, 207, 378, 1980
Tru1I TTAA 7 cut(s) 360, 1539, 1791, 1815, 2322, 2649, 2741
Tru9I TTAA 7 cut(s) 360, 1539, 1791, 1815, 2322, 2649, 2741
TscAI CASTG 9 cut(s) 372, 490, 546, 858, 1144, 1780, 1930, 2004, 2100
TseFI GTSAC 3 cut(s) 1130, 1685, 1895
Tsp45I GTSAC 3 cut(s) 1130, 1685, 1895
TspGWI ACGGA 4 cut(s) 32, 101, 2189, 2259
TspRI CASTG 9 cut(s) 372, 490, 546, 858, 1144, 1780, 1930, 2004, 2100
Vha464I CTTAAG 1 cut(s) 359
VneI GTGCAC 1 cut(s) 368
VpaK11BI GGWCC 6 cut(s) 881, 1033, 2173, 2197, 2545, 2614
VspI ATTAAT 1 cut(s) 2741
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 6 cut(s) 464, 559, 733, 1343, 1593, 1962
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 2367
XmaJI CCTAGG 1 cut(s) 2284
XmiI GTMKAC 1 cut(s) 1941
XspI CTAG 7 cut(s) 141, 438, 458, 1307, 1683, 2285, 2415
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.