Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0010284

Physical Location & Seq

Genomic Coordinates Forward (+)

3640409 .. 3652653

Gene Structure 12245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g04200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGAAGCCCTCAGTCTCCCTCCACCCCCCTACCATGGCCCTAGCTTCCGGTCTCCGCTTGTCTTCCTCCATCCTCCGCTACCGCCTCGCCGCCCCGCTCAACCCGACCCGACTCTCCGGTCTCCGCCACAACCTCTCGGAGCTCCGCTTCCTTTCAGCATCATCCTCCCGCCCCAGGACCCGCGTCCGCCCAATTAAAGCGACGCTCCTTGACGAACCCCAAAAGGAGGACCCCCACGAGGGGCAAGATGGGAACGGGAGTGTGCTGGTCAAGGACACAGGCGAGAATAGCGAAAGGATTGTCCGTGTTGAGCTCCACAAGGAGGCCACTGAAGCCTACATGGCCTACGCTATGTCTGTCCTGCTCGGCCGCGCCCTGCCCGATGTTCGTGATGGTTTGAAGCCCGTGCACCGCCGGATTTTGTATGCAATGCACGAGTTGGGGATTGTGTCACGGAAACCGTTCAAGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTCCATCCCCATGGAGACACTGCTGTGTATGATTCGTTGGTGCGAATGGCTCAGGATTTCTCCTTACGATTCCCACTAATCCAAGGGCATGGGAATTTTGGTTCTATTGATGCTGATCCTGCAGCTGCCATGCGTTACACGGAATGCAGATTGGAAGCACTGACAGAAGCAATGCTGTTGGCAGATTTAGATCAAAATACGGTTGATTTTACTCCAAATTTTGATAATTCACAAAAAGAGCCATCAGTTTTACCTGCTCGACTTCCAACTTTATTACTGAATGGTGCTTCTGGGATTGCGGTCGGCATGGCAACAAACATTCCTCCGCATAATCTTGGGGAATTGGTAGATGTTCTTAGCGTGTTAATTCACAATCCAGAAGCCACGTTGCAAGAATTACTAGAATACATGCCAGGACCCGATTTTCCAACTGGAGGACTGATCATGGGCAACCTTGGAATCTTAGACGCATATCGAACCGGAAAAGGACGTATTGTTGTAAGAGGGAAAACTGATGTTGAATTGCTTGATTCTAAAACAAAGAGAAATGCAGTTATTATAAAAGAGATCCCTTACCAAACCAACAAAGCAGCTCTTGTGGAGAAGATAGCGGAACTTGTGGAAAATAAGATTTTAGAAGGCATAAGTGATATTAGGGACGAAAGTGATCGTTCTGGAATGCGTGTAGTTATTGAGCTCAAGCGAGGATCCGACCCCTCAATTGTTTTAAATAATCTCTACCGCCTCACATCTCTTCAATGCAGTTTTAGTTGTAACATGGTGGGTATTCATAATGGGCAACCTAAGCAGATGGGTCTAAAGGAATTGCTGCAGGCATTCTTAGACTTCAGATGCTCTGTTGTTGAGAGACGTGCTAAGTTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGCATTGTTGTGGGCCTTGATAATTTGGATGCGGTAATTCGTATACTAAGGGAAGCTTCGAGCAATGCAATTGCATCAGCTGGTTTAAGGACTGAGTTCAATCTTTCTGAGAAACAAGCTGAAGCCATATTAGATATTAGCCTAAGAAGAATTACCCAGCTTGAGAGGAAAAAGTTTGTAAATGAAAGTGAATCGTTAAAGGAGCAAATTTCAAAATTAGAACAACTATTGTCAAGCAAGAAATCCATGTTACAGTTGATTGAACAAGAAGCAAACGAGTTAAAAAGCAAGTTTTCCAGTCCGAGGCGTTCTGTGCTAGAGGACAGTGATGGTGGCCACGTTGATGACATAGATGTTATTCCTAATGAAGAAATGCTTTTGGCCTTCAGTGAAAAGGGCTATGTTAAGCGGATGAAACCTAACACTTTCAATCTTCAAAATAGAGGAACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAATGATGCAATGTCAGACTTTATTGTTTGTCGCGCACATGATCATGTTCTATACTTTAGTGATAAAGGAACAGTTTACTCTGGTCGTGCGTATAAAATTCCTGAATGCACCCGCACAGCTGCTGGGACGCCATTGGTTCAGATCTTAGCACTGTCAGATGGTGAAAGGATTACTTCTGTTATTCCTGTGAGTGAATTTGCTGGAGATCAGTTTCTACTTATGCTTACAGTGAATGGATACATCAAGAAGGAGATCGGAAGATCTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0009295 GO:0009507 GO:0009536 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseA_C DNA_topoisoIV

Protein Analysis

725

Amino Acids

80.2

Weight (kDa)

8.58

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_topoisoIV	PF00521	124 - 565	1.6e-159	DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C	PF03989	599 - 645	1.5e-08	DNA gyrase C-terminal domain, beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10690
fragaria_vesca	FvH4_7g05000 FvH4_7g05000
malus_domestica	MD02G1265100.v1.1 MD07G1054300.v1.1
prunus_persica	Prupe.2G060900_v2.0.a1 Prupe.2G060900_v2.0.a1
pyrus_communis	pycom02g22650 pycom07g04200
rosa_chinensis	RchiOBHm_Chr1g0331271
rosa_laevigata	RLG00000029745
rosa_multiflora	Rmu_co8191516.1_g000001 Rmu_sc0000597.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319250
rosa_rugosa	Rorug01G0092400 Rorug01G0092500
rosa_samantha	Rh1AG117100 Rh1BG087900 Rh1CG112100 Rh1DG120900
rosa_wichuraiana	Rw1G009370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1064
AasI	GACNNNNNNGTC	1 cut(s)	117
Acc36I	ACCTGC	1 cut(s)	766
AccBSI	CCGCTC	1 cut(s)	97
AccI	GTMKAC	1 cut(s)	1468
AccII	CGCG	3 cut(s)	183, 372, 1938
AclWI	GGATC	4 cut(s)	614, 1066, 1206, 1219
AcoI	YGGCCR	2 cut(s)	367, 1759
AcsI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
AcuI	CTGAAG	4 cut(s)	351, 1336, 1566, 1795
AcyI	GRCGYC	1 cut(s)	2033
AfiI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 240, 322, 938, 1728
AjiI	CACGTC	1 cut(s)	1376
AjnI	CCWGG	2 cut(s)	173, 916
AleI	CACNNNNGTG	1 cut(s)	527
AloI	GAACNNNNNNTCC	2 cut(s)	589, 621
Alw21I	GWGCWC	4 cut(s)	144, 315, 411, 1203
Alw26I	GTCTC	5 cut(s)	19, 56, 125, 513, 1366
Alw44I	GTGCAC	1 cut(s)	407
AlwI	GGATC	4 cut(s)	614, 1066, 1206, 1219
AlwNI	CAGNNNCTG	1 cut(s)	2027
AoxI	GGCC	7 cut(s)	36, 324, 342, 367, 1438, 1759, 1806
ApaLI	GTGCAC	1 cut(s)	407
ApeKI	GCWGC	5 cut(s)	626, 629, 1094, 1333, 2024
ApoI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
ArsI	GACNNNNNNTTYG	2 cut(s)	285, 317
Asp700I	GAANNNNTTC	1 cut(s)	461
AspLEI	GCGC	2 cut(s)	374, 1940
AspS9I	GGNCC	5 cut(s)	37, 177, 229, 920, 1438
AsuHPI	GGTGA	1 cut(s)	2078
AvaII	GGWCC	3 cut(s)	177, 229, 920
BaeGI	GKGCMC	1 cut(s)	411
BalI	TGGCCA	1 cut(s)	1761
BamHI	GGATCC	1 cut(s)	1211
BanII	GRGCYC	3 cut(s)	144, 315, 1203
BauI	CACGAG	2 cut(s)	236, 434
BbsI	GAAGAC	2 cut(s)	54, 1417
Bbv12I	GWGCWC	4 cut(s)	144, 315, 411, 1203
BbvI	GCAGC	5 cut(s)	616, 638, 1106, 1320, 2011
BccI	CCATC	8 cut(s)	77, 242, 386, 516, 754, 1309, 1748, 2057
BciT130I	CCWGG	2 cut(s)	175, 918
BciVI	GTATCC	1 cut(s)	2135
BclI	TGATCA	2 cut(s)	945, 1945
BcoDI	GTCTC	5 cut(s)	19, 56, 125, 513, 1366
BfaI	CTAG	5 cut(s)	41, 477, 497, 905, 1742
BfmI	CTRYAG	2 cut(s)	624, 1334
BfuAI	ACCTGC	1 cut(s)	766
BfuI	GTATCC	1 cut(s)	2135
BglI	GCCNNNNNGGC	1 cut(s)	341
BglII	AGATCT	2 cut(s)	2046, 2165
BisI	GCNGC	7 cut(s)	90, 370, 627, 630, 1095, 1334, 2025
BlsI	GCNGC	7 cut(s)	91, 371, 628, 631, 1096, 1335, 2026
Bme1390I	CCNGG	2 cut(s)	175, 918
Bme18I	GGWCC	3 cut(s)	177, 229, 920
BmgBI	CACGTC	1 cut(s)	1376
BmgT120I	GGNCC	5 cut(s)	37, 177, 229, 920, 1438
BmiI	GGNNCC	5 cut(s)	179, 231, 922, 1213, 1873
BmrFI	CCNGG	2 cut(s)	175, 918
BmsI	GCATC	6 cut(s)	167, 604, 1346, 1444, 1508, 1900
BoxI	GACNNNNGTC	1 cut(s)	182
BpiI	GAAGAC	2 cut(s)	54, 1417
BpmI	CTGGAG	2 cut(s)	957, 2127
Bpu10I	CCTNAGC	2 cut(s)	555, 1308
BpuEI	CTTGAG	2 cut(s)	1187, 1607
BsaBI	GATNNNNATC	2 cut(s)	618, 693
BsaHI	GRCGYC	1 cut(s)	2033
BsaI	GGTCTC	2 cut(s)	56, 125
BsaJI	CCNNGG	6 cut(s)	33, 173, 514, 586, 958, 1727
BsaWI	WCCGGW	3 cut(s)	47, 116, 983
Bsc4I	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 240, 322, 938, 1728
Bse1I	ACTGG	2 cut(s)	940, 1722
Bse3DI	GCAATG	4 cut(s)	435, 681, 1495, 1920
Bse8I	GATNNNNATC	2 cut(s)	618, 693
BseBI	CCWGG	2 cut(s)	175, 918
BseDI	CCNNGG	6 cut(s)	33, 173, 514, 586, 958, 1727
BseGI	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BseJI	GATNNNNATC	2 cut(s)	618, 693
BseLI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 240, 322, 938, 1728
BseMI	GCAATG	4 cut(s)	435, 681, 1495, 1920
BseMII	CTCAG	4 cut(s)	24, 569, 1509, 1524
BseNI	ACTGG	2 cut(s)	940, 1722
BseSI	GKGCMC	1 cut(s)	411
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	5 cut(s)	616, 638, 1106, 1320, 2011
BseYI	CCCAGC	2 cut(s)	1581, 2027
Bsh1236I	CGCG	3 cut(s)	183, 372, 1938
Bsh1285I	CGRYCG	2 cut(s)	370, 807
BshFI	GGCC	7 cut(s)	38, 326, 344, 369, 1440, 1761, 1808
BsiEI	CGRYCG	2 cut(s)	370, 807
BsiHKAI	GWGCWC	4 cut(s)	144, 315, 411, 1203
BsiSI	CCGG	4 cut(s)	48, 117, 415, 984
BslFI	GGGAC	2 cut(s)	1175, 2044
BslI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 240, 322, 938, 1728
BsmAI	GTCTC	5 cut(s)	19, 56, 125, 513, 1366
BsmBI	CGTCTC	1 cut(s)	1366
BsmFI	GGGAC	2 cut(s)	1175, 2044
BsmI	GAATGC	4 cut(s)	653, 1188, 1340, 2015
BsnI	GGCC	7 cut(s)	38, 326, 344, 369, 1440, 1761, 1808
Bso31I	GGTCTC	2 cut(s)	56, 125
Bsp1286I	GDGCHC	4 cut(s)	144, 315, 411, 1203
Bsp19I	CCATGG	2 cut(s)	33, 514
BspANI	GGCC	7 cut(s)	38, 326, 344, 369, 1440, 1761, 1808
BspCNI	CTCAG	4 cut(s)	23, 568, 1510, 1525
BspFNI	CGCG	3 cut(s)	183, 372, 1938
BspLI	GGNNCC	5 cut(s)	179, 231, 922, 1213, 1873
BspMAI	CTGCAG	2 cut(s)	628, 1338
BspMI	ACCTGC	1 cut(s)	766
BspPI	GGATC	4 cut(s)	614, 1066, 1206, 1219
BspTNI	GGTCTC	2 cut(s)	56, 125
BsrBI	CCGCTC	1 cut(s)	97
BsrDI	GCAATG	4 cut(s)	435, 681, 1495, 1920
BsrI	ACTGG	2 cut(s)	940, 1722
BssECI	CCNNGG	6 cut(s)	33, 173, 514, 586, 958, 1727
BssNAI	GTATAC	1 cut(s)	1469
BssNI	GRCGYC	1 cut(s)	2033
BssSI	CACGAG	2 cut(s)	236, 434
BssT1I	CCWWGG	4 cut(s)	33, 514, 586, 958
Bst1107I	GTATAC	1 cut(s)	1469
Bst2BI	CACGAG	2 cut(s)	236, 434
Bst2UI	CCWGG	2 cut(s)	175, 918
Bst4CI	ACNGT	7 cut(s)	462, 706, 1680, 1751, 1978, 2058, 2134
Bst6I	CTCTTC	1 cut(s)	1263
BstACI	GRCGYC	1 cut(s)	2033
BstC8I	GCNNGC	1 cut(s)	1338
BstDSI	CCRYGG	2 cut(s)	33, 514
BstF5I	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BstFNI	CGCG	3 cut(s)	183, 372, 1938
BstHHI	GCGC	2 cut(s)	374, 1940
BstMAI	GTCTC	5 cut(s)	19, 56, 125, 513, 1366
BstMCI	CGRYCG	2 cut(s)	370, 807
BstMWI	GCNNNNNNNGC	6 cut(s)	288, 332, 341, 623, 1309, 1738
BstNI	CCWGG	2 cut(s)	175, 918
BstNSI	RCATGY	1 cut(s)	916
BstPAI	GACNNNNGTC	1 cut(s)	182
BstSCI	CCNGG	2 cut(s)	173, 916
BstSFI	CTRYAG	2 cut(s)	624, 1334
BstSLI	GKGCMC	1 cut(s)	411
BstUI	CGCG	3 cut(s)	183, 372, 1938
BstV1I	GCAGC	5 cut(s)	616, 638, 1106, 1320, 2011
BstV2I	GAAGAC	2 cut(s)	54, 1417
BstX2I	RGATCY	4 cut(s)	1071, 1211, 2046, 2165
BstXI	CCANNNNNNTGG	2 cut(s)	515, 593
BstYI	RGATCY	4 cut(s)	1071, 1211, 2046, 2165
BstZ17I	GTATAC	1 cut(s)	1469
BstZI	CGGCCG	1 cut(s)	367
BsuI	GTATCC	1 cut(s)	2135
BsuRI	GGCC	7 cut(s)	38, 326, 344, 369, 1440, 1761, 1808
BtgI	CCRYGG	2 cut(s)	33, 514
BtrI	CACGTC	1 cut(s)	1376
BtsCI	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BtsI	GCAGTG	1 cut(s)	522
BtsIMutI	CAGTG	7 cut(s)	327, 522, 662, 1756, 1819, 2054, 2139
BveI	ACCTGC	1 cut(s)	766
Cac8I	GCNNGC	1 cut(s)	1338
CaiI	CAGNNNCTG	1 cut(s)	2027
CfoI	GCGC	2 cut(s)	374, 1940
Cfr13I	GGNCC	5 cut(s)	37, 177, 229, 920, 1438
CseI	GACGC	4 cut(s)	172, 211, 980, 2041
DraI	TTTAAA	1 cut(s)	1233
DrdI	GACNNNNNNGTC	1 cut(s)	117
DseDI	GACNNNNNNGTC	1 cut(s)	117
EaeI	YGGCCR	2 cut(s)	367, 1759
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	1263
EarI	CTCTTC	1 cut(s)	1263
EciI	GGCGGA	2 cut(s)	113, 176
Ecl136II	GAGCTC	3 cut(s)	142, 313, 1201
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	4 cut(s)	33, 514, 586, 958
Eco24I	GRGCYC	3 cut(s)	144, 315, 1203
Eco31I	GGTCTC	2 cut(s)	56, 125
Eco47I	GGWCC	3 cut(s)	177, 229, 920
Eco52I	CGGCCG	1 cut(s)	367
Eco53kI	GAGCTC	3 cut(s)	142, 313, 1201
Eco57I	CTGAAG	4 cut(s)	351, 1336, 1566, 1795
EcoICRI	GAGCTC	3 cut(s)	142, 313, 1201
EcoO109I	RGGNCCY	3 cut(s)	177, 229, 920
EcoRII	CCWGG	2 cut(s)	173, 916
EcoT14I	CCWWGG	4 cut(s)	33, 514, 586, 958
EcoT38I	GRGCYC	3 cut(s)	144, 315, 1203
ErhI	CCWWGG	4 cut(s)	33, 514, 586, 958
Esp3I	CGTCTC	1 cut(s)	1366
FalI	AAGNNNNNCTT	8 cut(s)	1083, 1115, 1465, 1497, 1785, 1817, 2062, 2094
FaqI	GGGAC	2 cut(s)	1175, 2044
FauI	CCCGC	4 cut(s)	102, 176, 188, 2024
FbaI	TGATCA	2 cut(s)	945, 1945
FblI	GTMKAC	1 cut(s)	1468
Fnu4HI	GCNGC	7 cut(s)	90, 370, 627, 630, 1095, 1334, 2025
FokI	GGATG	5 cut(s)	56, 148, 495, 1466, 1849
FriOI	GRGCYC	3 cut(s)	144, 315, 1203
Fsp4HI	GCNGC	7 cut(s)	90, 370, 627, 630, 1095, 1334, 2025
FspBI	CTAG	5 cut(s)	41, 477, 497, 905, 1742
GlaI	GCGC	2 cut(s)	373, 1939
GluI	GCNGC	7 cut(s)	90, 370, 627, 630, 1095, 1334, 2025
GsaI	CCCAGC	2 cut(s)	1585, 2031
GsuI	CTGGAG	2 cut(s)	957, 2127
HaeIII	GGCC	7 cut(s)	38, 326, 344, 369, 1440, 1761, 1808
HapII	CCGG	4 cut(s)	48, 117, 415, 984
HgaI	GACGC	4 cut(s)	172, 211, 980, 2041
HhaI	GCGC	2 cut(s)	374, 1940
Hin1I	GRCGYC	1 cut(s)	2033
Hin6I	GCGC	2 cut(s)	372, 1938
HinP1I	GCGC	2 cut(s)	372, 1938
HindIII	AAGCTT	1 cut(s)	1479
HinfI	GANTC	6 cut(s)	111, 536, 573, 963, 1034, 1616
HpaII	CCGG	4 cut(s)	48, 117, 415, 984
HphI	GGTGA	1 cut(s)	2078
Hpy166II	GTNNAC	3 cut(s)	409, 1469, 1981
Hpy188I	TCNGA	9 cut(s)	139, 1216, 1355, 1534, 1728, 1921, 2046, 2062, 2162
Hpy188III	TCNNGA	7 cut(s)	389, 466, 557, 881, 1179, 2006, 2149
Hpy8I	GTNNAC	3 cut(s)	409, 1469, 1981
Hpy99I	CGWCG	1 cut(s)	205
HpyAV	CCTTC	3 cut(s)	1136, 1819, 2146
HpyCH4III	ACNGT	7 cut(s)	462, 706, 1680, 1751, 1978, 2058, 2134
HpyCH4IV	ACGT	4 cut(s)	890, 994, 1375, 1764
HpyF10VI	GCNNNNNNNGC	6 cut(s)	288, 332, 341, 623, 1309, 1738
HpySE526I	ACGT	4 cut(s)	890, 994, 1375, 1764
Hsp92I	GRCGYC	1 cut(s)	2033
HspAI	GCGC	2 cut(s)	372, 1938
Ksp22I	TGATCA	2 cut(s)	945, 1945
LmnI	GCTCC	5 cut(s)	139, 147, 210, 318, 1627
Lsp1109I	GCAGC	5 cut(s)	616, 638, 1106, 1320, 2011
LweI	GCATC	6 cut(s)	167, 604, 1346, 1444, 1508, 1900
MaeI	CTAG	5 cut(s)	41, 477, 497, 905, 1742
MaeII	ACGT	4 cut(s)	890, 994, 1375, 1764
MaeIII	GTNAC	4 cut(s)	450, 638, 1277, 1674
MbiI	CCGCTC	1 cut(s)	97
MboII	GAAGA	8 cut(s)	54, 1120, 1250, 1422, 1584, 1805, 1850, 2175
MfeI	CAATTG	2 cut(s)	1224, 1494
MflI	RGATCY	4 cut(s)	1071, 1211, 2046, 2165
MhlI	GDGCHC	4 cut(s)	144, 315, 411, 1203
MlsI	TGGCCA	1 cut(s)	1761
MluNI	TGGCCA	1 cut(s)	1761
MlyI	GAGTC	1 cut(s)	105
MmeI	TCCRAC	4 cut(s)	466, 794, 956, 1239
Mox20I	TGGCCA	1 cut(s)	1761
MroXI	GAANNNNTTC	1 cut(s)	461
MscI	TGGCCA	1 cut(s)	1761
MseI	TTAA	7 cut(s)	195, 869, 1232, 1511, 1622, 1706, 1830
MslI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
Msp20I	TGGCCA	1 cut(s)	1761
MspA1I	CMGCKG	3 cut(s)	629, 1505, 2024
MspI	CCGG	4 cut(s)	48, 117, 415, 984
MspR9I	CCNGG	2 cut(s)	175, 918
MunI	CAATTG	2 cut(s)	1224, 1494
Mva1269I	GAATGC	4 cut(s)	653, 1188, 1340, 2015
MvaI	CCWGG	2 cut(s)	175, 918
MvnI	CGCG	3 cut(s)	183, 372, 1938
MwoI	GCNNNNNNNGC	6 cut(s)	288, 332, 341, 623, 1309, 1738
NcoI	CCATGG	2 cut(s)	33, 514
NlaIV	GGNNCC	5 cut(s)	179, 231, 922, 1213, 1873
NmeAIII	GCCGAG	1 cut(s)	345
NmuCI	GTSAC	1 cut(s)	450
NspI	RCATGY	1 cut(s)	916
OliI	CACNNNNGTG	1 cut(s)	527
PctI	GAATGC	4 cut(s)	653, 1188, 1340, 2015
PdmI	GAANNNNTTC	1 cut(s)	461
PfeI	GAWTC	5 cut(s)	536, 573, 963, 1034, 1616
PkrI	GCNGC	7 cut(s)	91, 371, 628, 631, 1096, 1335, 2026
PleI	GAGTC	1 cut(s)	105
PpsI	GAGTC	1 cut(s)	105
PpuMI	RGGWCCY	3 cut(s)	177, 229, 920
PshAI	GACNNNNGTC	1 cut(s)	182
PsiI	TTATAA	1 cut(s)	1064
Psp124BI	GAGCTC	3 cut(s)	144, 315, 1203
Psp5II	RGGWCCY	3 cut(s)	177, 229, 920
Psp6I	CCWGG	2 cut(s)	173, 916
PspFI	CCCAGC	2 cut(s)	1581, 2027
PspGI	CCWGG	2 cut(s)	173, 916
PspN4I	GGNNCC	5 cut(s)	179, 231, 922, 1213, 1873
PspPI	GGNCC	5 cut(s)	37, 177, 229, 920, 1438
PspPPI	RGGWCCY	3 cut(s)	177, 229, 920
PstI	CTGCAG	2 cut(s)	628, 1338
PstNI	CAGNNNCTG	1 cut(s)	2027
PsuI	RGATCY	4 cut(s)	1071, 1211, 2046, 2165
PvuII	CAGCTG	3 cut(s)	629, 1505, 2024
RseI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
SacI	GAGCTC	3 cut(s)	144, 315, 1203
SaqAI	TTAA	7 cut(s)	195, 869, 1232, 1511, 1622, 1706, 1830
SatI	GCNGC	7 cut(s)	90, 370, 627, 630, 1095, 1334, 2025
Sau96I	GGNCC	5 cut(s)	37, 177, 229, 920, 1438
SchI	GAGTC	1 cut(s)	105
ScrFI	CCNGG	2 cut(s)	175, 918
SduI	GDGCHC	4 cut(s)	144, 315, 411, 1203
SfaNI	GCATC	6 cut(s)	167, 604, 1346, 1444, 1508, 1900
SfcI	CTRYAG	2 cut(s)	624, 1334
SinI	GGWCC	3 cut(s)	177, 229, 920
SmiMI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
SmlI	CTYRAG	2 cut(s)	1202, 1586
SmoI	CTYRAG	2 cut(s)	1202, 1586
SspMI	CTAG	5 cut(s)	41, 477, 497, 905, 1742
SstI	GAGCTC	3 cut(s)	144, 315, 1203
StyD4I	CCNGG	2 cut(s)	173, 916
StyI	CCWWGG	4 cut(s)	33, 514, 586, 958
TaaI	ACNGT	7 cut(s)	462, 706, 1680, 1751, 1978, 2058, 2134
TaiI	ACGT	4 cut(s)	893, 997, 1378, 1767
TaqI	TCGA	3 cut(s)	763, 979, 1484
TauI	GCSGC	2 cut(s)	92, 372
TfiI	GAWTC	5 cut(s)	536, 573, 963, 1034, 1616
Tru1I	TTAA	7 cut(s)	195, 869, 1232, 1511, 1622, 1706, 1830
Tru9I	TTAA	7 cut(s)	195, 869, 1232, 1511, 1622, 1706, 1830
TscAI	CASTG	7 cut(s)	334, 529, 669, 1756, 1819, 2061, 2139
TseFI	GTSAC	1 cut(s)	450
TseI	GCWGC	5 cut(s)	626, 629, 1094, 1333, 2024
Tsp45I	GTSAC	1 cut(s)	450
TspDTI	ATGAA	5 cut(s)	17, 1283, 1623, 1806, 1853
TspGWI	ACGGA	3 cut(s)	293, 469, 659
TspRI	CASTG	7 cut(s)	334, 529, 669, 1756, 1819, 2061, 2139
VneI	GTGCAC	1 cut(s)	407
VpaK11BI	GGWCC	3 cut(s)	177, 229, 920
XapI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
XceI	RCATGY	1 cut(s)	916
XmiI	GTMKAC	1 cut(s)	1468
XmnI	GAANNNNTTC	1 cut(s)	461
XspI	CTAG	5 cut(s)	41, 477, 497, 905, 1742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.