FvH4_7g05000

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010284
Physical Location & Seq
Forward (+)
5426392 .. 5441502
15111 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05000.t1

Sequence Viewer

Length: 2865 bp
ATGAAATCCTCAGCCTCGGCCATAACCCCAACCCCAACCCCCACCATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCACTTTCCTCCGTTGCCGCCTCCCCGCACCGCTCAACCCGACCCGCTTCTCCGCCCTCCGCCACTCCCTCTTCGACCTCCGGTTCCTCTCTGCTTCCTCCTCCTCTCGTACTCGTACTCGGACCCGGCTCCGCCCCCGCCCCATCAAAGCGACGCCCAGCGAAGACGGCGGCCTCCTCGAAGGCGCCGGCGAGGGGCAAAATGGGAATGGGAGCGTCCTGGTCAAGGACTCGGACGAGCGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCCGTGCTGCTCGGCCGTGCCCTGCCGGACATTCGCGACGGTCTCAAGCCGGTCCACCGCCGTATTTTGTATGCAATGCATGAATTGAGGCTTTCGTCGCGAAAACCGTTTGTGAAATGTGCTAGAGTTGTGGGGGAGGTTCTAGGAAAATTCCATCCTCATGGTGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCCTTACGATCACCACTGATTAACGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACTGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGACTTTGTACCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGGGTTCCAAATTTATTATTAAATGGTTCTTCTGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAGTTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTAGAATACATGCCAGGACCTGATTTTCCAACTGGAGGACTGATTATGGGAAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATAGTGGTCAGAGGAAAAACTGATATAGAATCTCTTGATTCTAAAGGCAAGCGAAGTGCGATCATCATAAAGGAGATCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATAGCGCAACTTGTAGAGAATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTCATTGAGCTTAAGCGAGGGTCTGACCCATCTATTGTTCTCAATAATCTATATCGCCTCACATCACTCCAGTCCAGCTTTAGCTGTAACATGGTTGGTATCCTCAATGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCTTTCCTAGATTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATAGCTGTAGGCCTTGATAATCTGCAAAGAGTTATGCGTATAAGTTTGGAAGCTTCTAATAATACAATTGCATCATCTCTTCTAAGGAATGAGTTCAACCTTTCTGAGAAACAAGCTGAAGCAATATTAGACTTCAACTTTAGAAGGCTTAATGTTCTGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCATTGAAAGAGCAGATTTCAAAACTAGAAGAACTGTTGTCAAGCAAGAAACGTATATTGCAGGTGGTTGAACAAGAAGCGATTGACATAAAAAACAAGTTTGCTAATCCCAGGCGTTCAATGCTGGAAGACTCTGATGGTGGTCAACTTGATGATATTGATGTTATTCCGAATGACGAAATGCTTTTGGCCTTCAGTGAAAAAGGGTATGTCAAGCGGATGAAGCCTAACACATTTAATCTGCAAAATCGAGGAACCATTGGTAAATCTGTTGGTAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCATGCACATGATCATGTTTTGTTCTTCAGTGATAAAGGAACGGTCTACTCAGCTCGTGCGTATAAAATTCCTGAGTGCAGCCGAACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACTTCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAACGGCTACATAAAGAAGGTGTCTTTGAGCTCTTTTTCATCGATCAGGTCAACCGGAATCATAGCAATTCAATTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGATCTTGTAGCAATGGCATCCCTGAACGGAATGGTTATCTTATGTTCCTCTGATATTATTCGAGCTTTAGGCAGGAACACTCGGGGTTCTGTAGCCATGCGACTTAAAGAAGGTGATAAGATGGCAAGCGTGGACATTATACCAGCAGCTATGTGGAAAGACTTAAAAAGGGTGTCAGAAGCACCAGAGAGCACGGCTAGAAGTTTGGATGGCCCGTGGTTGTTGTTTGTGTCTGAGAGTGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCCTCAGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTCTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAGAGTGGTACAGTAAATAGAATTAAGGTGCGAGATATCTCAATACAGTCTCGCTATGCAAGGGGTGTTATTTTGATGCGGCTTGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATGTCAGCAACAGATGAGGAGCCAGAGAATGAAGTCGATGCTGAAGCTGTGATTCATGGTTGA

Protein Analysis

955

Amino Acids

105.17

Weight (kDa)

8.46

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 131 - 572 4.1e-156 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 606 - 652 2.5e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 656 - 706 3.1e-09 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 713 - 756 3.9e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 760 - 806 4e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 890 - 932 6.4e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1690
Acc36I ACCTGC 2 cut(s) 787, 1690
AccB1I GGYRCC 1 cut(s) 260
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 2001
AccII CGCG 2 cut(s) 411, 475
AclWI GGATC 2 cut(s) 635, 1087
AcoI YGGCCR 2 cut(s) 18, 388
AcsI RAATTY 5 cut(s) 524, 619, 793, 1403, 2022
AcuI CTGAAG 5 cut(s) 711, 1587, 1816, 1966, 2865
AcyI GRCGYC 2 cut(s) 230, 261
AdeI CACNNNGTG 1 cut(s) 669
AfaI GTAC 5 cut(s) 187, 193, 738, 2053, 2695
AfiI CCNNNNNNNGG 5 cut(s) 46, 270, 301, 343, 959
AflII CTTAAG 1 cut(s) 1223
AgsI TTSAA 9 cut(s) 630, 1408, 1546, 1585, 1645, 1659, 1709, 1758, 2228
AjiI CACGTC 1 cut(s) 1397
AjnI CCWGG 4 cut(s) 294, 937, 1748, 2236
AleI CACNNNNGTG 1 cut(s) 548
AloI GAACNNNNNNTCC 6 cut(s) 610, 642, 2517, 2549, 2658, 2690
Alw21I GWGCWC 3 cut(s) 336, 2189, 2471
Alw26I GTCTC 4 cut(s) 68, 422, 1387, 2739
AlwI GGATC 2 cut(s) 635, 1087
AlwNI CAGNNNCTG 2 cut(s) 944, 2048
Ama87I CYCGRG 2 cut(s) 783, 2358
AoxI GGCC 9 cut(s) 18, 48, 247, 345, 363, 388, 1459, 1827, 2488
ApoI RAATTY 5 cut(s) 524, 619, 793, 1403, 2022
AseI ATTAAT 1 cut(s) 2801
Asp700I GAANNNNTTC 2 cut(s) 1541, 2098
AspLEI GCGC 3 cut(s) 263, 685, 1138
AspS9I GGNCC 8 cut(s) 198, 427, 941, 2233, 2257, 2489, 2605, 2674
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 551, 588, 2252, 2402, 2666
AvaI CYCGRG 2 cut(s) 783, 2358
AvaII GGWCC 7 cut(s) 198, 427, 941, 2233, 2257, 2605, 2674
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 967, 1000
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 2 cut(s) 336, 2189
BauI CACGAG 1 cut(s) 2010
BbsI GAAGAC 3 cut(s) 246, 1438, 1773
Bbv12I GWGCWC 3 cut(s) 336, 2189, 2471
BbvCI CCTCAGC 1 cut(s) 10
BceAI ACGGC 5 cut(s) 259, 375, 420, 2176, 2487
BciT130I CCWGG 4 cut(s) 296, 939, 1750, 2238
BciVI GTATCC 2 cut(s) 715, 1322
BclI TGATCA 1 cut(s) 1966
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 4 cut(s) 68, 422, 1387, 2739
BfaI CTAG 7 cut(s) 498, 518, 926, 1367, 1664, 2475, 2678
BfmI CTRYAG 4 cut(s) 645, 1355, 1455, 2367
BfoI RGCGCY 2 cut(s) 264, 686
BfrI CTTAAG 1 cut(s) 1223
BfuAI ACCTGC 2 cut(s) 787, 1690
BfuI GTATCC 2 cut(s) 715, 1322
BglI GCCNNNNNGGC 1 cut(s) 362
BglII AGATCT 1 cut(s) 2067
Bme1390I CCNGG 5 cut(s) 202, 296, 939, 1750, 2238
Bme18I GGWCC 7 cut(s) 198, 427, 941, 2233, 2257, 2605, 2674
BmeT110I CYCGRG 2 cut(s) 783, 2358
BmgBI CACGTC 1 cut(s) 1397
BmgT120I GGNCC 8 cut(s) 198, 427, 941, 2233, 2257, 2489, 2605, 2674
BmiI GGNNCC 9 cut(s) 161, 200, 206, 262, 789, 1894, 2235, 2258, 2823
BmrFI CCNGG 5 cut(s) 202, 296, 939, 1750, 2238
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 8 cut(s) 343, 625, 1367, 1529, 1921, 2303, 2751, 2830
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 3 cut(s) 246, 1438, 1773
BpmI CTGGAG 4 cut(s) 978, 1265, 1628, 2148
Bpu10I CCTNAGC 3 cut(s) 10, 52, 576
BpuEI CTTGAG 2 cut(s) 404, 2560
BpuMI CCSGG 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 2198
BsaBI GATNNNNATC 1 cut(s) 639
BsaHI GRCGYC 2 cut(s) 230, 261
BsaI GGTCTC 2 cut(s) 68, 422
BsaJI CCNNGG 7 cut(s) 15, 45, 561, 979, 1748, 2236, 2492
BsaWI WCCGGW 3 cut(s) 59, 156, 2210
BsaXI ACNNNNNCTCC 2 cut(s) 1000, 1030
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 270, 301, 343, 959
Bse118I RCCGGY 2 cut(s) 263, 424
Bse1I ACTGG 5 cut(s) 961, 1009, 1204, 1282, 2064
Bse3DI GCAATG 4 cut(s) 456, 702, 1941, 2295
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 4 cut(s) 296, 939, 1750, 2238
BseCI ATCGAT 1 cut(s) 2198
BseDI CCNNGG 7 cut(s) 15, 45, 561, 979, 1748, 2236, 2492
BseGI GGATG 7 cut(s) 529, 880, 1189, 1863, 2294, 2491, 2656
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 5 cut(s) 46, 270, 301, 343, 959
BseMI GCAATG 4 cut(s) 456, 702, 1941, 2295
BseMII CTCAG 7 cut(s) 24, 590, 1545, 2019, 2019, 2502, 2613
BseNI ACTGG 5 cut(s) 961, 1009, 1204, 1282, 2064
BseRI GAGGAG 4 cut(s) 166, 169, 242, 2834
BseSI GKGCMC 1 cut(s) 397
BseX3I CGGCCG 1 cut(s) 388
BseYI CCCAGC 1 cut(s) 233
BsgI GTGCAG 3 cut(s) 691, 2053, 2251
Bsh1236I CGCG 2 cut(s) 411, 475
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 9 cut(s) 20, 50, 249, 347, 365, 390, 1461, 1829, 2490
BshNI GGYRCC 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 2198
BsiEI CGRYCG 1 cut(s) 391
BsiHKAI GWGCWC 3 cut(s) 336, 2189, 2471
BsiHKCI CYCGRG 2 cut(s) 783, 2358
BsiSI CCGG 7 cut(s) 60, 157, 202, 264, 401, 425, 2211
BslFI GGGAC 1 cut(s) 2219
BslI CCNNNNNNNGG 5 cut(s) 46, 270, 301, 343, 959
BsmAI GTCTC 4 cut(s) 68, 422, 1387, 2739
BsmBI CGTCTC 1 cut(s) 1387
BsmFI GGGAC 1 cut(s) 2219
BsmI GAATGC 1 cut(s) 1209
BsnI GGCC 9 cut(s) 20, 50, 249, 347, 365, 390, 1461, 1829, 2490
Bso31I GGTCTC 2 cut(s) 68, 422
BsoBI CYCGRG 2 cut(s) 783, 2358
Bsp1286I GDGCHC 4 cut(s) 336, 397, 2189, 2471
Bsp19I CCATGG 1 cut(s) 45
Bsp68I TCGCGA 2 cut(s) 411, 475
BspANI GGCC 9 cut(s) 20, 50, 249, 347, 365, 390, 1461, 1829, 2490
BspCNI CTCAG 7 cut(s) 23, 589, 1546, 2018, 2020, 2503, 2612
BspDI ATCGAT 1 cut(s) 2198
BspFNI CGCG 2 cut(s) 411, 475
BspLI GGNNCC 9 cut(s) 161, 200, 206, 262, 789, 1894, 2235, 2258, 2823
BspMAI CTGCAG 2 cut(s) 649, 1359
BspMI ACCTGC 2 cut(s) 787, 1690
BspPI GGATC 2 cut(s) 635, 1087
BspT107I GGYRCC 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 1223
BspTNI GGTCTC 2 cut(s) 68, 422
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 4 cut(s) 456, 702, 1941, 2295
BsrFI RCCGGY 2 cut(s) 263, 424
BsrI ACTGG 5 cut(s) 961, 1009, 1204, 1282, 2064
BssAI RCCGGY 2 cut(s) 263, 424
BssECI CCNNGG 7 cut(s) 15, 45, 561, 979, 1748, 2236, 2492
BssNI GRCGYC 2 cut(s) 230, 261
BssSI CACGAG 1 cut(s) 2010
BssT1I CCWWGG 3 cut(s) 45, 561, 979
Bst2BI CACGAG 1 cut(s) 2010
Bst2UI CCWGG 4 cut(s) 296, 939, 1750, 2238
Bst4CI ACNGT 8 cut(s) 416, 483, 727, 1674, 1999, 2155, 2698, 2733
Bst6I CTCTTC 4 cut(s) 79, 152, 1533, 2560
BstACI GRCGYC 2 cut(s) 230, 261
BstAFI CTTAAG 1 cut(s) 1223
BstAPI GCANNNNNTGC 1 cut(s) 922
BstC8I GCNNGC 4 cut(s) 265, 1067, 1359, 2404
BstDSI CCRYGG 2 cut(s) 45, 2492
BstENI CCTNNNNNAGG 1 cut(s) 299
BstF5I GGATG 7 cut(s) 529, 880, 1189, 1863, 2294, 2491, 2656
BstFNI CGCG 2 cut(s) 411, 475
BstH2I RGCGCY 2 cut(s) 264, 686
BstHHI GCGC 3 cut(s) 263, 685, 1138
BstMAI GTCTC 4 cut(s) 68, 422, 1387, 2739
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 8 cut(s) 243, 353, 362, 472, 644, 922, 1759, 1861
BstNI CCWGG 4 cut(s) 296, 939, 1750, 2238
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 5 cut(s) 200, 294, 937, 1748, 2236
BstSFI CTRYAG 4 cut(s) 645, 1355, 1455, 2367
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 2 cut(s) 411, 475
BstV2I GAAGAC 3 cut(s) 246, 1438, 1773
BstX2I RGATCY 2 cut(s) 1092, 2067
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 2 cut(s) 1092, 2067
BstZI CGGCCG 1 cut(s) 388
Bsu15I ATCGAT 1 cut(s) 2198
BsuI GTATCC 2 cut(s) 715, 1322
BsuRI GGCC 9 cut(s) 20, 50, 249, 347, 365, 390, 1461, 1829, 2490
BsuTUI ATCGAT 1 cut(s) 2198
BtgI CCRYGG 2 cut(s) 45, 2492
BtrI CACGTC 1 cut(s) 1397
BtsCI GGATG 7 cut(s) 529, 880, 1189, 1863, 2294, 2491, 2656
BtsI GCAGTG 2 cut(s) 543, 911
BtsIMutI CAGTG 8 cut(s) 543, 599, 911, 1197, 1840, 1990, 2057, 2160
BtuMI TCGCGA 2 cut(s) 411, 475
BveI ACCTGC 2 cut(s) 787, 1690
Cac8I GCNNGC 4 cut(s) 265, 1067, 1359, 2404
CaiI CAGNNNCTG 2 cut(s) 944, 2048
CfoI GCGC 3 cut(s) 263, 685, 1138
Cfr10I RCCGGY 2 cut(s) 263, 424
Cfr13I GGNCC 8 cut(s) 198, 427, 941, 2233, 2257, 2489, 2605, 2674
ClaI ATCGAT 1 cut(s) 2198
CseI GACGC 2 cut(s) 238, 280
Csp6I GTAC 5 cut(s) 186, 192, 737, 2052, 2694
CviQI GTAC 5 cut(s) 186, 192, 737, 2052, 2694
DinI GGCGCC 1 cut(s) 262
DraIII CACNNNGTG 1 cut(s) 669
EaeI YGGCCR 2 cut(s) 18, 388
EagI CGGCCG 1 cut(s) 388
Eam1104I CTCTTC 4 cut(s) 79, 152, 1533, 2560
EarI CTCTTC 4 cut(s) 79, 152, 1533, 2560
EciI GGCGGA 4 cut(s) 56, 118, 125, 197
Ecl136II GAGCTC 2 cut(s) 334, 2187
EclXI CGGCCG 1 cut(s) 388
Eco130I CCWWGG 3 cut(s) 45, 561, 979
Eco147I AGGCCT 1 cut(s) 1461
Eco24I GRGCYC 2 cut(s) 336, 2189
Eco31I GGTCTC 2 cut(s) 68, 422
Eco32I GATATC 1 cut(s) 2722
Eco47I GGWCC 7 cut(s) 198, 427, 941, 2233, 2257, 2605, 2674
Eco52I CGGCCG 1 cut(s) 388
Eco53kI GAGCTC 2 cut(s) 334, 2187
Eco57I CTGAAG 5 cut(s) 711, 1587, 1816, 1966, 2865
Eco88I CYCGRG 2 cut(s) 783, 2358
EcoICRI GAGCTC 2 cut(s) 334, 2187
EcoNI CCTNNNNNAGG 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 941
EcoRII CCWGG 4 cut(s) 294, 937, 1748, 2236
EcoRV GATATC 1 cut(s) 2722
EcoT14I CCWWGG 3 cut(s) 45, 561, 979
EcoT22I ATGCAT 1 cut(s) 456
EcoT38I GRGCYC 2 cut(s) 336, 2189
EgeI GGCGCC 1 cut(s) 262
EheI GGCGCC 1 cut(s) 262
ErhI CCWWGG 3 cut(s) 45, 561, 979
Esp3I CGTCTC 1 cut(s) 1387
FaqI GGGAC 1 cut(s) 2219
FauI CCCGC 3 cut(s) 109, 128, 221
FbaI TGATCA 1 cut(s) 1966
FblI GTMKAC 1 cut(s) 2001
FokI GGATG 7 cut(s) 516, 887, 1196, 1870, 2281, 2498, 2663
FriOI GRGCYC 2 cut(s) 336, 2189
FspBI CTAG 7 cut(s) 498, 518, 926, 1367, 1664, 2475, 2678
GlaI GCGC 3 cut(s) 262, 684, 1137
GsaI CCCAGC 1 cut(s) 237
GsuI CTGGAG 4 cut(s) 978, 1265, 1628, 2148
HaeII RGCGCY 2 cut(s) 264, 686
HaeIII GGCC 9 cut(s) 20, 50, 249, 347, 365, 390, 1461, 1829, 2490
HapII CCGG 7 cut(s) 60, 157, 202, 264, 401, 425, 2211
HgaI GACGC 2 cut(s) 238, 280
HhaI GCGC 3 cut(s) 263, 685, 1138
Hin1I GRCGYC 2 cut(s) 230, 261
Hin6I GCGC 3 cut(s) 261, 683, 1136
HinP1I GCGC 3 cut(s) 261, 683, 1136
HincII GTYRAC 4 cut(s) 730, 1325, 1784, 2208
HindII GTYRAC 4 cut(s) 730, 1325, 1784, 2208
HindIII AAGCTT 1 cut(s) 1500
HpaII CCGG 7 cut(s) 60, 157, 202, 264, 401, 425, 2211
HphI GGTGA 5 cut(s) 551, 588, 2252, 2402, 2666
Hpy166II GTNNAC 9 cut(s) 430, 730, 1325, 1784, 1927, 2002, 2158, 2208, 2410
Hpy188III TCNNGA 7 cut(s) 410, 474, 578, 902, 1052, 1607, 2027
Hpy8I GTNNAC 9 cut(s) 430, 730, 1325, 1784, 1927, 2002, 2158, 2208, 2410
Hpy99I CGWCG 3 cut(s) 232, 416, 475
HpyAV CCTTC 5 cut(s) 251, 1587, 1840, 2167, 2381
HpyCH4III ACNGT 8 cut(s) 416, 483, 727, 1674, 1999, 2155, 2698, 2733
HpyCH4IV ACGT 3 cut(s) 1015, 1396, 1690
HpyF10VI GCNNNNNNNGC 8 cut(s) 243, 353, 362, 472, 644, 922, 1759, 1861
HpySE526I ACGT 3 cut(s) 1015, 1396, 1690
Hsp92I GRCGYC 2 cut(s) 230, 261
HspAI GCGC 3 cut(s) 261, 683, 1136
KasI GGCGCC 1 cut(s) 260
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 1966
LmnI GCTCC 4 cut(s) 210, 288, 339, 2821
LweI GCATC 8 cut(s) 343, 625, 1367, 1529, 1921, 2303, 2751, 2830
MaeI CTAG 7 cut(s) 498, 518, 926, 1367, 1664, 2475, 2678
MaeII ACGT 3 cut(s) 1015, 1396, 1690
MaeIII GTNAC 5 cut(s) 539, 659, 1190, 1298, 2585
MbiI CCGCTC 1 cut(s) 109
MfeI CAATTG 3 cut(s) 630, 1515, 2228
MflI RGATCY 2 cut(s) 1092, 2067
MhlI GDGCHC 4 cut(s) 336, 397, 2189, 2471
Mly113I GGCGCC 1 cut(s) 261
MlyI GAGTC 2 cut(s) 299, 1763
MmeI TCCRAC 2 cut(s) 977, 2611
Mph1103I ATGCAT 1 cut(s) 456
MreI CGCCGGCG 1 cut(s) 263
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 2 cut(s) 1541, 2098
MseI TTAA 9 cut(s) 606, 803, 1224, 1599, 1875, 2382, 2441, 2709, 2801
MslI CAYNNNNRTG 5 cut(s) 534, 548, 1202, 1962, 1968
MspA1I CMGCKG 2 cut(s) 650, 2048
MspCI CTTAAG 1 cut(s) 1223
MspI CCGG 7 cut(s) 60, 157, 202, 264, 401, 425, 2211
MspR9I CCNGG 5 cut(s) 202, 296, 939, 1750, 2238
MunI CAATTG 3 cut(s) 630, 1515, 2228
Mva1269I GAATGC 1 cut(s) 1209
MvaI CCWGG 4 cut(s) 296, 939, 1750, 2238
MvnI CGCG 2 cut(s) 411, 475
MwoI GCNNNNNNNGC 8 cut(s) 243, 353, 362, 472, 644, 922, 1759, 1861
NaeI GCCGGC 1 cut(s) 265
NarI GGCGCC 1 cut(s) 261
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 45
NgoMIV GCCGGC 1 cut(s) 263
NlaIV GGNNCC 9 cut(s) 161, 200, 206, 262, 789, 1894, 2235, 2258, 2823
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 539, 1190
NruI TCGCGA 2 cut(s) 411, 475
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 1 cut(s) 937
OliI CACNNNNGTG 1 cut(s) 548
PaqCI CACCTGC 1 cut(s) 1690
PceI AGGCCT 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 479
PctI GAATGC 1 cut(s) 1209
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 1541, 2098
PleI GAGTC 2 cut(s) 299, 1763
PluTI GGCGCC 1 cut(s) 264
PpsI GAGTC 2 cut(s) 299, 1763
PpuMI RGGWCCY 1 cut(s) 941
PshBI ATTAAT 1 cut(s) 2801
Psp124BI GAGCTC 2 cut(s) 336, 2189
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 4 cut(s) 294, 937, 1748, 2236
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 4 cut(s) 294, 937, 1748, 2236
PspN4I GGNNCC 9 cut(s) 161, 200, 206, 262, 789, 1894, 2235, 2258, 2823
PspPI GGNCC 8 cut(s) 198, 427, 941, 2233, 2257, 2489, 2605, 2674
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 2 cut(s) 649, 1359
PstNI CAGNNNCTG 2 cut(s) 944, 2048
PsuI RGATCY 2 cut(s) 1092, 2067
PvuII CAGCTG 2 cut(s) 650, 2048
RruI TCGCGA 2 cut(s) 411, 475
RsaI GTAC 5 cut(s) 187, 193, 738, 2053, 2695
RsaNI GTAC 5 cut(s) 186, 192, 737, 2052, 2694
RseI CAYNNNNRTG 5 cut(s) 534, 548, 1202, 1962, 1968
SacI GAGCTC 2 cut(s) 336, 2189
SaqAI TTAA 9 cut(s) 606, 803, 1224, 1599, 1875, 2382, 2441, 2709, 2801
Sau96I GGNCC 8 cut(s) 198, 427, 941, 2233, 2257, 2489, 2605, 2674
SchI GAGTC 2 cut(s) 299, 1763
ScrFI CCNGG 5 cut(s) 202, 296, 939, 1750, 2238
SduI GDGCHC 4 cut(s) 336, 397, 2189, 2471
SfaNI GCATC 8 cut(s) 343, 625, 1367, 1529, 1921, 2303, 2751, 2830
SfcI CTRYAG 4 cut(s) 645, 1355, 1455, 2367
SfoI GGCGCC 1 cut(s) 262
SgrAI CRCCGGYG 1 cut(s) 263
SinI GGWCC 7 cut(s) 198, 427, 941, 2233, 2257, 2605, 2674
SmiMI CAYNNNNRTG 5 cut(s) 534, 548, 1202, 1962, 1968
SmlI CTYRAG 3 cut(s) 419, 1223, 2539
SmoI CTYRAG 3 cut(s) 419, 1223, 2539
SseBI AGGCCT 1 cut(s) 1461
SspDI GGCGCC 1 cut(s) 260
SspI AATATT 1 cut(s) 1575
SspMI CTAG 7 cut(s) 498, 518, 926, 1367, 1664, 2475, 2678
SstI GAGCTC 2 cut(s) 336, 2189
StuI AGGCCT 1 cut(s) 1461
StyD4I CCNGG 5 cut(s) 200, 294, 937, 1748, 2236
StyI CCWWGG 3 cut(s) 45, 561, 979
TaaI ACNGT 8 cut(s) 416, 483, 727, 1674, 1999, 2155, 2698, 2733
TaiI ACGT 3 cut(s) 1018, 1399, 1693
TaqI TCGA 7 cut(s) 150, 255, 1000, 1888, 2198, 2338, 2838
TaqII GACCGA 1 cut(s) 2622
TauI GCSGC 3 cut(s) 96, 249, 2767
Tru1I TTAA 9 cut(s) 606, 803, 1224, 1599, 1875, 2382, 2441, 2709, 2801
Tru9I TTAA 9 cut(s) 606, 803, 1224, 1599, 1875, 2382, 2441, 2709, 2801
TscAI CASTG 8 cut(s) 550, 606, 918, 1204, 1840, 1990, 2064, 2160
TseFI GTSAC 2 cut(s) 539, 1190
Tsp45I GTSAC 2 cut(s) 539, 1190
TspGWI ACGGA 4 cut(s) 77, 367, 2249, 2319
TspRI CASTG 8 cut(s) 550, 606, 918, 1204, 1840, 1990, 2064, 2160
Vha464I CTTAAG 1 cut(s) 1223
VpaK11BI GGWCC 7 cut(s) 198, 427, 941, 2233, 2257, 2605, 2674
VspI ATTAAT 1 cut(s) 2801
XagI CCTNNNNNAGG 1 cut(s) 299
XapI RAATTY 5 cut(s) 524, 619, 793, 1403, 2022
XceI RCATGY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 2427
XmiI GTMKAC 1 cut(s) 2001
XmnI GAANNNNTTC 2 cut(s) 1541, 2098
XspI CTAG 7 cut(s) 498, 518, 926, 1367, 1664, 2475, 2678
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.