Rroxscaffold_4G00319250

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010284
Physical Location & Seq
Reverse (-)
46807485 .. 46822507
15023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319250.1

Sequence Viewer

Length: 3069 bp
ATGATCGGTAGGTTTAGCCGAGTTCAACAAGCCAAGAAACTTGAAGATTGGTGGTCGGAATCGAGAGATATCGTCGTTGACTCAAAATCGAGCTCAAATCGGACCGAGCTTGCCGCCGCCTCTCTACGGCCGGAGTCGCCGTACATGGCCGCCACGGACGGGTGCGCAAGGACCATACGAAGCCAATGGAAGAAGTTTGGTGAGGATTGGTGGCCGGAGGAGGAAGATATGGCCGGTGGAAGTTTTCGGGCGATTTCCGCCGAAACCCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCGCCGCGACGACGACGACAACCTCCTCGCAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCCGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCATCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAGTTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTCAGAGCTGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATCGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTTATGCTTGTGTAATCAATAATGTTTTGGCATATGTCTATGTGGTCAAGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTGCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTTGTAGAGGATAAGATTTTAGATGGCATAAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGGAGTTCGCATACTTTTGTCGAAATGGATCAGCAAAAGACTGTTTGCAATTGGATAATGATTCTTTCTCATCCTCTTTCAGCTTAAGCGAGGATCCGACCCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGTATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTGGATAATCTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAAGGGTATGTTAAGCGGATGAAGCCTAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACGTCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTAGGGGTTCTGTAGCCATGCGACTTAAAGAAGGTGATAAGATGGCAAGCGTAGACATTATACCAGCAGCTATGCGGAAAGATTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCGGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCTATACAGTCCCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGTAATTCATGGTTAA

Protein Analysis

1022

Amino Acids

113.27

Weight (kDa)

6.22

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 197 - 467 5.3e-94 DNA gyrase/topoisomerase IV, subunit A
DNA_topoisoIV PF00521 494 - 659 5.8e-30 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 693 - 739 2.7e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 743 - 793 3.8e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 828 - 874 4.3e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 900 - 945 1e-05 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 958 - 1003 2.9e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1951
Acc16I TGCGCA 1 cut(s) 166
Acc36I ACCTGC 2 cut(s) 994, 1951
Acc65I GGTACC 1 cut(s) 2153
AccB1I GGYRCC 3 cut(s) 429, 2153, 3025
AccBSI CCGCTC 2 cut(s) 321, 414
AccI GTMKAC 2 cut(s) 2262, 2613
AccII CGCG 3 cut(s) 435, 609, 1031
AclWI GGATC 6 cut(s) 786, 833, 1034, 1435, 1487, 1500
AcoI YGGCCR 5 cut(s) 128, 147, 212, 231, 586
AcsI RAATTY 5 cut(s) 817, 1000, 1664, 1914, 2283
AcuI CTGAAG 6 cut(s) 1005, 1537, 1618, 1834, 2077, 2227
AcyI GRCGYC 1 cut(s) 430
AdeI CACNNNGTG 1 cut(s) 867
AfaI GTAC 7 cut(s) 143, 397, 579, 2155, 2240, 2314, 2899
AfiI CCNNNNNNNGG 6 cut(s) 126, 159, 499, 541, 1166, 2553
AflII CTTAAG 2 cut(s) 617, 1483
AflIII ACRYGT 1 cut(s) 2130
AjiI CACGTC 2 cut(s) 1272, 1658
AjnI CCWGG 3 cut(s) 1144, 1722, 2440
AleI CACNNNNGTG 1 cut(s) 746
AloI GAACNNNNNNTCC 6 cut(s) 808, 840, 2721, 2753, 2862, 2894
Alw21I GWGCWC 3 cut(s) 95, 534, 630
Alw26I GTCTC 3 cut(s) 280, 732, 1648
Alw44I GTGCAC 1 cut(s) 626
AlwI GGATC 6 cut(s) 786, 833, 1034, 1435, 1487, 1500
AlwNI CAGNNNCTG 2 cut(s) 1151, 2309
ApaLI GTGCAC 1 cut(s) 626
ApeKI GCWGC 9 cut(s) 845, 848, 1294, 1615, 2295, 2306, 2469, 2627, 2819
ApoI RAATTY 5 cut(s) 817, 1000, 1664, 1914, 2283
AseI ATTAAT 1 cut(s) 3005
Asp718I GGTACC 1 cut(s) 2153
AspA2I CCTAGG 1 cut(s) 2548
AspLEI GCGC 3 cut(s) 167, 432, 883
AspS9I GGNCC 9 cut(s) 102, 171, 402, 1148, 2437, 2461, 2693, 2809, 2878
AsuHPI GGTGA 4 cut(s) 212, 2456, 2606, 2870
AvaII GGWCC 7 cut(s) 102, 171, 1148, 2437, 2461, 2809, 2878
AvrII CCTAGG 1 cut(s) 2548
BaeGI GKGCMC 2 cut(s) 595, 630
BamHI GGATCC 1 cut(s) 1492
BanI GGYRCC 3 cut(s) 429, 2153, 3025
BanII GRGCYC 3 cut(s) 95, 534, 2548
BauI CACGAG 1 cut(s) 2271
BbsI GAAGAC 1 cut(s) 1699
Bbv12I GWGCWC 3 cut(s) 95, 534, 630
BbvCI CCTCAGC 1 cut(s) 1827
BbvI GCAGC 9 cut(s) 835, 857, 1306, 1602, 2307, 2318, 2456, 2639, 2831
BceAI ACGGC 4 cut(s) 124, 143, 573, 2691
BciT130I CCWGG 3 cut(s) 1146, 1724, 2442
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 1 cut(s) 2227
BcoDI GTCTC 3 cut(s) 280, 732, 1648
BfaI CTAG 9 cut(s) 399, 696, 716, 1628, 2004, 2549, 2562, 2679, 2882
BfmI CTRYAG 4 cut(s) 843, 1616, 1716, 2571
BfoI RGCGCY 2 cut(s) 433, 884
BfrI CTTAAG 2 cut(s) 617, 1483
BfuAI ACCTGC 2 cut(s) 994, 1951
BfuI GTATCC 1 cut(s) 913
BglI GCCNNNNNGGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 2328
BlnI CCTAGG 1 cut(s) 2548
Bme1390I CCNGG 3 cut(s) 1146, 1724, 2442
Bme18I GGWCC 7 cut(s) 102, 171, 1148, 2437, 2461, 2809, 2878
BmgBI CACGTC 2 cut(s) 1272, 1658
BmgT120I GGNCC 9 cut(s) 102, 171, 402, 1148, 2437, 2461, 2693, 2809, 2878
BmiI GGNNCC 7 cut(s) 404, 431, 1494, 2155, 2439, 2462, 3027
BmrFI CCNGG 3 cut(s) 1146, 1724, 2442
BmrI ACTGGG 1 cut(s) 1270
BmsI GCATC 8 cut(s) 541, 823, 1628, 1790, 2010, 2182, 2507, 2955
BmuI ACTGGG 1 cut(s) 1270
BpiI GAAGAC 1 cut(s) 1699
BpmI CTGGAG 4 cut(s) 1185, 1526, 2045, 2409
Bpu10I CCTNAGC 2 cut(s) 774, 1827
BpuEI CTTGAG 1 cut(s) 2764
BsaBI GATNNNNATC 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 430
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 6 cut(s) 153, 759, 1186, 2440, 2548, 2696
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 8 cut(s) 125, 155, 343, 373, 1256, 1286, 2639, 2669
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 159, 499, 541, 1166, 2553
Bse118I RCCGGY 1 cut(s) 233
Bse1I ACTGG 5 cut(s) 1168, 1265, 1384, 1543, 2325
Bse3DI GCAATG 3 cut(s) 654, 900, 2202
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 3 cut(s) 1146, 1724, 2442
BseDI CCNNGG 6 cut(s) 153, 759, 1186, 2440, 2548, 2696
BseGI GGATG 8 cut(s) 668, 727, 1087, 1369, 1469, 2124, 2498, 2860
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 6 cut(s) 126, 159, 499, 541, 1166, 2553
BseMI GCAATG 3 cut(s) 654, 900, 2202
BseMII CTCAG 8 cut(s) 393, 519, 788, 1806, 1818, 2280, 2280, 2706
BseNI ACTGG 5 cut(s) 1168, 1265, 1384, 1543, 2325
BseRI GAGGAG 5 cut(s) 233, 379, 443, 1182, 1415
BseSI GKGCMC 2 cut(s) 595, 630
BseX3I CGGCCG 2 cut(s) 128, 586
BseXI GCAGC 9 cut(s) 835, 857, 1306, 1602, 2307, 2318, 2456, 2639, 2831
BseYI CCCAGC 1 cut(s) 405
BsgI GTGCAG 4 cut(s) 299, 889, 2314, 2455
Bsh1236I CGCG 3 cut(s) 435, 609, 1031
Bsh1285I CGRYCG 2 cut(s) 131, 589
BshNI GGYRCC 3 cut(s) 429, 2153, 3025
BsiEI CGRYCG 2 cut(s) 131, 589
BsiHKAI GWGCWC 3 cut(s) 95, 534, 630
BsiSI CCGG 5 cut(s) 131, 215, 234, 272, 599
BslFI GGGAC 2 cut(s) 2423, 2923
BslI CCNNNNNNNGG 6 cut(s) 126, 159, 499, 541, 1166, 2553
BsmAI GTCTC 3 cut(s) 280, 732, 1648
BsmBI CGTCTC 1 cut(s) 1648
BsmFI GGGAC 2 cut(s) 2423, 2923
BsmI GAATGC 3 cut(s) 636, 1389, 1622
Bso31I GGTCTC 1 cut(s) 280
Bsp1286I GDGCHC 5 cut(s) 95, 534, 595, 630, 2548
Bsp68I TCGCGA 1 cut(s) 609
BspCNI CTCAG 8 cut(s) 392, 518, 787, 1807, 1819, 2279, 2281, 2707
BspFNI CGCG 3 cut(s) 435, 609, 1031
BspLI GGNNCC 7 cut(s) 404, 431, 1494, 2155, 2439, 2462, 3027
BspMAI CTGCAG 2 cut(s) 847, 1620
BspMI ACCTGC 2 cut(s) 994, 1951
BspPI GGATC 6 cut(s) 786, 833, 1034, 1435, 1487, 1500
BspT107I GGYRCC 3 cut(s) 429, 2153, 3025
BspTI CTTAAG 2 cut(s) 617, 1483
BspTNI GGTCTC 1 cut(s) 280
BsrBI CCGCTC 2 cut(s) 321, 414
BsrDI GCAATG 3 cut(s) 654, 900, 2202
BsrFI RCCGGY 1 cut(s) 233
BsrI ACTGG 5 cut(s) 1168, 1265, 1384, 1543, 2325
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 6 cut(s) 153, 759, 1186, 2440, 2548, 2696
BssNI GRCGYC 1 cut(s) 430
BssSI CACGAG 1 cut(s) 2271
BssT1I CCWWGG 3 cut(s) 759, 1186, 2548
Bst2BI CACGAG 1 cut(s) 2271
Bst2UI CCWGG 3 cut(s) 1146, 1724, 2442
Bst4CI ACNGT 8 cut(s) 614, 925, 1442, 1583, 2260, 2416, 2902, 2937
Bst6I CTCTTC 3 cut(s) 291, 1794, 2764
BstACI GRCGYC 1 cut(s) 430
BstAFI CTTAAG 2 cut(s) 617, 1483
BstAPI GCANNNNNTGC 1 cut(s) 1129
BstC8I GCNNGC 5 cut(s) 111, 460, 991, 1620, 2608
BstDSI CCRYGG 2 cut(s) 153, 2696
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 8 cut(s) 668, 727, 1087, 1369, 1469, 2124, 2498, 2860
BstFNI CGCG 3 cut(s) 435, 609, 1031
BstH2I RGCGCY 2 cut(s) 433, 884
BstHHI GCGC 3 cut(s) 167, 432, 883
BstMAI GTCTC 3 cut(s) 280, 732, 1648
BstMCI CGRYCG 2 cut(s) 131, 589
BstNI CCWGG 3 cut(s) 1146, 1724, 2442
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 3 cut(s) 1144, 1722, 2440
BstSFI CTRYAG 4 cut(s) 843, 1616, 1716, 2571
BstSLI GKGCMC 2 cut(s) 595, 630
BstUI CGCG 3 cut(s) 435, 609, 1031
BstV1I GCAGC 9 cut(s) 835, 857, 1306, 1602, 2307, 2318, 2456, 2639, 2831
BstV2I GAAGAC 1 cut(s) 1699
BstX2I RGATCY 2 cut(s) 1492, 2328
BstYI RGATCY 2 cut(s) 1492, 2328
BstZI CGGCCG 2 cut(s) 128, 586
BsuI GTATCC 1 cut(s) 913
BtgI CCRYGG 2 cut(s) 153, 2696
BtgZI GCGATG 1 cut(s) 2507
BtrI CACGTC 2 cut(s) 1272, 1658
BtsCI GGATG 8 cut(s) 668, 727, 1087, 1369, 1469, 2124, 2498, 2860
BtsI GCAGTG 2 cut(s) 741, 1118
BtsIMutI CAGTG 8 cut(s) 741, 797, 1118, 1377, 2101, 2251, 2318, 2421
BtuMI TCGCGA 1 cut(s) 609
BveI ACCTGC 2 cut(s) 994, 1951
Cac8I GCNNGC 5 cut(s) 111, 460, 991, 1620, 2608
CaiI CAGNNNCTG 2 cut(s) 1151, 2309
CfoI GCGC 3 cut(s) 167, 432, 883
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 9 cut(s) 102, 171, 402, 1148, 2437, 2461, 2693, 2809, 2878
CpoI CGGWCCG 1 cut(s) 102
CseI GACGC 1 cut(s) 478
Csp6I GTAC 7 cut(s) 142, 396, 578, 2154, 2239, 2313, 2898
CspI CGGWCCG 1 cut(s) 102
CviQI GTAC 7 cut(s) 142, 396, 578, 2154, 2239, 2313, 2898
DinI GGCGCC 1 cut(s) 431
DraIII CACNNNGTG 1 cut(s) 867
EaeI YGGCCR 5 cut(s) 128, 147, 212, 231, 586
EagI CGGCCG 2 cut(s) 128, 586
Eam1104I CTCTTC 3 cut(s) 291, 1794, 2764
EarI CTCTTC 3 cut(s) 291, 1794, 2764
EciI GGCGGA 3 cut(s) 247, 268, 337
Ecl136II GAGCTC 2 cut(s) 93, 532
EclXI CGGCCG 2 cut(s) 128, 586
Eco130I CCWWGG 3 cut(s) 759, 1186, 2548
Eco147I AGGCCT 1 cut(s) 1722
Eco24I GRGCYC 3 cut(s) 95, 534, 2548
Eco31I GGTCTC 1 cut(s) 280
Eco32I GATATC 2 cut(s) 70, 2926
Eco47I GGWCC 7 cut(s) 102, 171, 1148, 2437, 2461, 2809, 2878
Eco52I CGGCCG 2 cut(s) 128, 586
Eco53kI GAGCTC 2 cut(s) 93, 532
Eco57I CTGAAG 6 cut(s) 1005, 1537, 1618, 1834, 2077, 2227
EcoICRI GAGCTC 2 cut(s) 93, 532
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoO109I RGGNCCY 3 cut(s) 402, 1148, 2437
EcoRII CCWGG 3 cut(s) 1144, 1722, 2440
EcoRV GATATC 2 cut(s) 70, 2926
EcoT14I CCWWGG 3 cut(s) 759, 1186, 2548
EcoT22I ATGCAT 1 cut(s) 654
EcoT38I GRGCYC 3 cut(s) 95, 534, 2548
EgeI GGCGCC 1 cut(s) 431
EheI GGCGCC 1 cut(s) 431
ErhI CCWWGG 3 cut(s) 759, 1186, 2548
Esp3I CGTCTC 1 cut(s) 1648
FaqI GGGAC 2 cut(s) 2423, 2923
FauI CCCGC 3 cut(s) 340, 2913, 2948
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 1 cut(s) 2227
FblI GTMKAC 2 cut(s) 2262, 2613
FokI GGATG 8 cut(s) 655, 714, 1094, 1376, 1456, 2131, 2485, 2867
FriOI GRGCYC 3 cut(s) 95, 534, 2548
FspBI CTAG 9 cut(s) 399, 696, 716, 1628, 2004, 2549, 2562, 2679, 2882
FspI TGCGCA 1 cut(s) 166
GlaI GCGC 3 cut(s) 166, 431, 882
GsaI CCCAGC 1 cut(s) 409
GsuI CTGGAG 4 cut(s) 1185, 1526, 2045, 2409
HaeII RGCGCY 2 cut(s) 433, 884
HapII CCGG 5 cut(s) 131, 215, 234, 272, 599
HgaI GACGC 1 cut(s) 478
HhaI GCGC 3 cut(s) 167, 432, 883
Hin1I GRCGYC 1 cut(s) 430
Hin6I GCGC 3 cut(s) 165, 430, 881
HinP1I GCGC 3 cut(s) 165, 430, 881
HincII GTYRAC 3 cut(s) 79, 1586, 2045
HindII GTYRAC 3 cut(s) 79, 1586, 2045
HindIII AAGCTT 1 cut(s) 1761
HpaII CCGG 5 cut(s) 131, 215, 234, 272, 599
HphI GGTGA 4 cut(s) 212, 2456, 2606, 2870
Hpy166II GTNNAC 7 cut(s) 79, 628, 1586, 2045, 2188, 2263, 2614
Hpy188III TCNNGA 7 cut(s) 63, 608, 672, 685, 776, 1109, 2288
Hpy8I GTNNAC 7 cut(s) 79, 628, 1586, 2045, 2188, 2263, 2614
Hpy99I CGWCG 5 cut(s) 77, 440, 443, 446, 614
HpyAV CCTTC 5 cut(s) 1004, 1848, 2101, 2428, 2585
HpyCH4III ACNGT 8 cut(s) 614, 925, 1442, 1583, 2260, 2416, 2902, 2937
HpyCH4IV ACGT 5 cut(s) 603, 1271, 1657, 2132, 2360
HpySE526I ACGT 5 cut(s) 603, 1271, 1657, 2132, 2360
Hsp92I GRCGYC 1 cut(s) 430
HspAI GCGC 3 cut(s) 165, 430, 881
KasI GGCGCC 1 cut(s) 429
KpnI GGTACC 1 cut(s) 2157
Ksp22I TGATCA 1 cut(s) 2227
LmnI GCTCC 4 cut(s) 414, 419, 486, 537
Lsp1109I GCAGC 9 cut(s) 835, 857, 1306, 1602, 2307, 2318, 2456, 2639, 2831
LweI GCATC 8 cut(s) 541, 823, 1628, 1790, 2010, 2182, 2507, 2955
MaeI CTAG 9 cut(s) 399, 696, 716, 1628, 2004, 2549, 2562, 2679, 2882
MaeII ACGT 5 cut(s) 603, 1271, 1657, 2132, 2360
MaeIII GTNAC 7 cut(s) 301, 857, 1370, 1559, 2006, 2216, 2789
MbiI CCGCTC 2 cut(s) 321, 414
MfeI CAATTG 4 cut(s) 828, 1448, 1776, 1980
MflI RGATCY 2 cut(s) 1492, 2328
MhlI GDGCHC 5 cut(s) 95, 534, 595, 630, 2548
Mly113I GGCGCC 1 cut(s) 430
MlyI GAGTC 4 cut(s) 74, 143, 497, 2964
MmeI TCCRAC 6 cut(s) 36, 386, 685, 1184, 1520, 2815
Mph1103I ATGCAT 1 cut(s) 654
MseI TTAA 9 cut(s) 618, 1484, 1860, 2112, 2136, 2586, 2913, 3005, 3067
MslI CAYNNNNRTG 5 cut(s) 732, 746, 1382, 2223, 2229
MspA1I CMGCKG 3 cut(s) 848, 1557, 2309
MspCI CTTAAG 2 cut(s) 617, 1483
MspI CCGG 5 cut(s) 131, 215, 234, 272, 599
MspR9I CCNGG 3 cut(s) 1146, 1724, 2442
MunI CAATTG 4 cut(s) 828, 1448, 1776, 1980
Mva1269I GAATGC 3 cut(s) 636, 1389, 1622
MvaI CCWGG 3 cut(s) 1146, 1724, 2442
MvnI CGCG 3 cut(s) 435, 609, 1031
NarI GGCGCC 1 cut(s) 430
NdeI CATATG 1 cut(s) 1222
NlaIV GGNNCC 7 cut(s) 404, 431, 1494, 2155, 2439, 2462, 3027
NmeAIII GCCGAG 2 cut(s) 44, 564
NmuCI GTSAC 3 cut(s) 1370, 2006, 2216
NruI TCGCGA 1 cut(s) 609
NsbI TGCGCA 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 746
PaqCI CACCTGC 1 cut(s) 1951
PceI AGGCCT 1 cut(s) 1722
PcsI WCGNNNNNNNCGW 2 cut(s) 462, 516
PctI GAATGC 3 cut(s) 636, 1389, 1622
PfeI GAWTC 9 cut(s) 59, 516, 755, 802, 1099, 1460, 1898, 2838, 2989
PleI GAGTC 4 cut(s) 74, 142, 497, 2964
PluTI GGCGCC 1 cut(s) 433
PpsI GAGTC 4 cut(s) 74, 142, 497, 2964
PpuMI RGGWCCY 2 cut(s) 1148, 2437
PshBI ATTAAT 1 cut(s) 3005
Psp124BI GAGCTC 2 cut(s) 95, 534
Psp5II RGGWCCY 2 cut(s) 1148, 2437
Psp6I CCWGG 3 cut(s) 1144, 1722, 2440
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 3 cut(s) 1144, 1722, 2440
PspN4I GGNNCC 7 cut(s) 404, 431, 1494, 2155, 2439, 2462, 3027
PspPI GGNCC 9 cut(s) 102, 171, 402, 1148, 2437, 2461, 2693, 2809, 2878
PspPPI RGGWCCY 2 cut(s) 1148, 2437
PstI CTGCAG 2 cut(s) 847, 1620
PstNI CAGNNNCTG 2 cut(s) 1151, 2309
PsuI RGATCY 2 cut(s) 1492, 2328
PvuII CAGCTG 3 cut(s) 848, 1557, 2309
RruI TCGCGA 1 cut(s) 609
RsaI GTAC 7 cut(s) 143, 397, 579, 2155, 2240, 2314, 2899
RsaNI GTAC 7 cut(s) 142, 396, 578, 2154, 2239, 2313, 2898
RseI CAYNNNNRTG 5 cut(s) 732, 746, 1382, 2223, 2229
Rsr2I CGGWCCG 1 cut(s) 102
RsrII CGGWCCG 1 cut(s) 102
SacI GAGCTC 2 cut(s) 95, 534
SaqAI TTAA 9 cut(s) 618, 1484, 1860, 2112, 2136, 2586, 2913, 3005, 3067
Sau96I GGNCC 9 cut(s) 102, 171, 402, 1148, 2437, 2461, 2693, 2809, 2878
SchI GAGTC 4 cut(s) 74, 143, 497, 2964
ScrFI CCNGG 3 cut(s) 1146, 1724, 2442
SduI GDGCHC 5 cut(s) 95, 534, 595, 630, 2548
SfaNI GCATC 8 cut(s) 541, 823, 1628, 1790, 2010, 2182, 2507, 2955
SfcI CTRYAG 4 cut(s) 843, 1616, 1716, 2571
SfoI GGCGCC 1 cut(s) 431
SinI GGWCC 7 cut(s) 102, 171, 1148, 2437, 2461, 2809, 2878
SmiMI CAYNNNNRTG 5 cut(s) 732, 746, 1382, 2223, 2229
SmlI CTYRAG 3 cut(s) 617, 1483, 2743
SmoI CTYRAG 3 cut(s) 617, 1483, 2743
SseBI AGGCCT 1 cut(s) 1722
SspDI GGCGCC 1 cut(s) 429
SspI AATATT 1 cut(s) 1836
SspMI CTAG 9 cut(s) 399, 696, 716, 1628, 2004, 2549, 2562, 2679, 2882
SstI GAGCTC 2 cut(s) 95, 534
StuI AGGCCT 1 cut(s) 1722
StyD4I CCNGG 3 cut(s) 1144, 1722, 2440
StyI CCWWGG 3 cut(s) 759, 1186, 2548
TaaI ACNGT 8 cut(s) 614, 925, 1442, 1583, 2260, 2416, 2902, 2937
TaiI ACGT 5 cut(s) 606, 1274, 1660, 2135, 2363
TaqI TCGA 8 cut(s) 62, 89, 1256, 1420, 2018, 2149, 2542, 2973
TaqII GACCGA 3 cut(s) 119, 902, 2826
TatI WGTACW 2 cut(s) 577, 2238
TauI GCSGC 8 cut(s) 116, 119, 152, 344, 435, 465, 636, 2301
TfiI GAWTC 9 cut(s) 59, 516, 755, 802, 1099, 1460, 1898, 2838, 2989
Tru1I TTAA 9 cut(s) 618, 1484, 1860, 2112, 2136, 2586, 2913, 3005, 3067
Tru9I TTAA 9 cut(s) 618, 1484, 1860, 2112, 2136, 2586, 2913, 3005, 3067
TscAI CASTG 8 cut(s) 748, 804, 1125, 1384, 2101, 2251, 2325, 2421
TseFI GTSAC 3 cut(s) 1370, 2006, 2216
TseI GCWGC 9 cut(s) 845, 848, 1294, 1615, 2295, 2306, 2469, 2627, 2819
Tsp45I GTSAC 3 cut(s) 1370, 2006, 2216
TspGWI ACGGA 5 cut(s) 170, 289, 358, 2453, 2523
TspRI CASTG 8 cut(s) 748, 804, 1125, 1384, 2101, 2251, 2325, 2421
Vha464I CTTAAG 2 cut(s) 617, 1483
VneI GTGCAC 1 cut(s) 626
VpaK11BI GGWCC 7 cut(s) 102, 171, 1148, 2437, 2461, 2809, 2878
VspI ATTAAT 1 cut(s) 3005
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 5 cut(s) 817, 1000, 1664, 1914, 2283
XceI RCATGY 1 cut(s) 1144
XmaJI CCTAGG 1 cut(s) 2548
XmiI GTMKAC 2 cut(s) 2262, 2613
XspI CTAG 9 cut(s) 399, 696, 716, 1628, 2004, 2549, 2562, 2679, 2882
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.