Rh1BG087900

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010284
Physical Location & Seq
Forward (+)
13947820 .. 13962295
14476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG087900.1

Sequence Viewer

Length: 2928 bp
ATGCCAAAAGCAACCCCACAGCCTTGGTTTAGGGTTTTAGCTGTAAAAGCCCCAAATCCCAAACCCCTCCCGGGCCGCGGCTCTATGAAATCCTCAGTCTCGGCCATAACCCCTACCCCCAACATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTCCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCCCCGCGACGACGACGACGACCTCCTCGCAGGCGGCGAGGGGCAGAATGGGAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCCGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCGCTGACCGAAGCAATGTTGTTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATCGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCTTGATTCTATAGGCAAGCGAAGTGCAATTATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTTAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGTATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATCTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTTTGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAAGGGTATGTTAAGCGGATGAAGCCTAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTTTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTGTTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCAACCGGAATCATAGCAATTCAATTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTAGGGGTTCTGTTGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGACATTATACCAGCAGCCATGCGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCGGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCCCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGTAATTCATGGTTAA

Protein Analysis

975

Amino Acids

107.48

Weight (kDa)

8.48

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 152 - 593 5.3e-156 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 627 - 673 2.5e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 677 - 727 3.6e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 734 - 777 4e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 781 - 827 4.1e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 853 - 898 9.5e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 911 - 956 2.8e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1753
Acc36I ACCTGC 2 cut(s) 850, 1753
Acc65I GGTACC 1 cut(s) 1955
AccB1I GGYRCC 2 cut(s) 1955, 2884
AccBSI CCGCTC 2 cut(s) 187, 279
AccI GTMKAC 2 cut(s) 2064, 2472
AccII CGCG 4 cut(s) 78, 300, 474, 887
AclWI GGATC 5 cut(s) 651, 698, 890, 1290, 1303
AcoI YGGCCR 2 cut(s) 102, 451
AcsI RAATTY 6 cut(s) 587, 682, 856, 1466, 1716, 2085
AcuI CTGAAG 6 cut(s) 861, 1339, 1420, 1636, 1879, 2029
AdeI CACNNNGTG 1 cut(s) 732
AfaI GTAC 6 cut(s) 262, 444, 1957, 2042, 2116, 2758
AfiI CCNNNNNNNGG 7 cut(s) 71, 77, 124, 364, 406, 1022, 2412
AflII CTTAAG 2 cut(s) 482, 1286
AflIII ACRYGT 1 cut(s) 1932
AjiI CACGTC 1 cut(s) 1460
AjnI CCWGG 2 cut(s) 1000, 2299
AleI CACNNNNGTG 1 cut(s) 611
AloI GAACNNNNNNTCC 6 cut(s) 673, 705, 2580, 2612, 2721, 2753
Alw21I GWGCWC 2 cut(s) 399, 495
Alw26I GTCTC 4 cut(s) 103, 146, 597, 1450
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 5 cut(s) 651, 698, 890, 1290, 1303
AlwNI CAGNNNCTG 2 cut(s) 1007, 2111
Ama87I CYCGRG 1 cut(s) 70
ApaLI GTGCAC 1 cut(s) 491
ApeKI GCWGC 9 cut(s) 710, 713, 1178, 1417, 2097, 2108, 2328, 2486, 2678
ApoI RAATTY 6 cut(s) 587, 682, 856, 1466, 1716, 2085
AseI ATTAAT 1 cut(s) 2864
Asp718I GGTACC 1 cut(s) 1955
AspA2I CCTAGG 1 cut(s) 2407
AspLEI GCGC 1 cut(s) 748
AsuC2I CCSGG 2 cut(s) 71, 72
AsuHPI GGTGA 2 cut(s) 2315, 2729
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 6 cut(s) 1004, 1156, 2296, 2320, 2668, 2737
AvrII CCTAGG 1 cut(s) 2407
BaeGI GKGCMC 2 cut(s) 460, 495
BaeI ACNNNNGTAYC 2 cut(s) 1030, 1063
BamHI GGATCC 1 cut(s) 1295
BanI GGYRCC 2 cut(s) 1955, 2884
BanII GRGCYC 2 cut(s) 399, 2407
BauI CACGAG 1 cut(s) 2073
BbsI GAAGAC 1 cut(s) 1501
Bbv12I GWGCWC 2 cut(s) 399, 495
BbvCI CCTCAGC 1 cut(s) 1629
BbvI GCAGC 9 cut(s) 700, 722, 1190, 1404, 2109, 2120, 2315, 2498, 2690
BceAI ACGGC 2 cut(s) 438, 2550
BciT130I CCWGG 2 cut(s) 1002, 2301
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 2029
BcnI CCSGG 2 cut(s) 71, 72
BcoDI GTCTC 4 cut(s) 103, 146, 597, 1450
BfaI CTAG 9 cut(s) 264, 561, 581, 1430, 1806, 2408, 2421, 2538, 2741
BfmI CTRYAG 4 cut(s) 708, 1122, 1418, 1518
BfoI RGCGCY 1 cut(s) 749
BfrI CTTAAG 2 cut(s) 482, 1286
BfuAI ACCTGC 2 cut(s) 850, 1753
BfuI GTATCC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 2130
BlnI CCTAGG 1 cut(s) 2407
Bme1390I CCNGG 4 cut(s) 71, 72, 1002, 2301
Bme18I GGWCC 6 cut(s) 1004, 1156, 2296, 2320, 2668, 2737
BmeT110I CYCGRG 1 cut(s) 70
BmgBI CACGTC 1 cut(s) 1460
BmiI GGNNCC 8 cut(s) 269, 296, 1158, 1297, 1957, 2298, 2321, 2886
BmrFI CCNGG 4 cut(s) 71, 72, 1002, 2301
BmrI ACTGGG 1 cut(s) 1077
BmsI GCATC 8 cut(s) 406, 688, 1430, 1592, 1812, 1984, 2366, 2814
BmuI ACTGGG 1 cut(s) 1077
BpiI GAAGAC 1 cut(s) 1501
BpmI CTGGAG 4 cut(s) 1041, 1328, 1847, 2211
Bpu10I CCTNAGC 3 cut(s) 130, 639, 1629
BpuEI CTTGAG 1 cut(s) 2623
BpuMI CCSGG 2 cut(s) 71, 72
BsaBI GATNNNNATC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 8 cut(s) 23, 70, 76, 624, 1042, 2299, 2407, 2555
BsaWI WCCGGW 2 cut(s) 137, 2273
BsaXI ACNNNNNCTCC 6 cut(s) 209, 239, 1063, 1093, 2498, 2528
Bsc4I CCNNNNNNNGG 7 cut(s) 71, 77, 124, 364, 406, 1022, 2412
Bse1I ACTGG 5 cut(s) 1024, 1072, 1267, 1345, 2127
Bse3DI GCAATG 3 cut(s) 519, 765, 2004
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 2 cut(s) 1002, 2301
BseDI CCNNGG 8 cut(s) 23, 70, 76, 624, 1042, 2299, 2407, 2555
BseGI GGATG 6 cut(s) 592, 943, 1252, 1926, 2357, 2719
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 7 cut(s) 71, 77, 124, 364, 406, 1022, 2412
BseMI GCAATG 3 cut(s) 519, 765, 2004
BseMII CTCAG 9 cut(s) 108, 258, 384, 653, 1608, 1620, 2082, 2082, 2565
BseNI ACTGG 5 cut(s) 1024, 1072, 1267, 1345, 2127
BseRI GAGGAG 3 cut(s) 244, 308, 1038
BseSI GKGCMC 2 cut(s) 460, 495
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 9 cut(s) 700, 722, 1190, 1404, 2109, 2120, 2315, 2498, 2690
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 4 cut(s) 165, 754, 2116, 2314
Bsh1236I CGCG 4 cut(s) 78, 300, 474, 887
Bsh1285I CGRYCG 1 cut(s) 454
BshNI GGYRCC 2 cut(s) 1955, 2884
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 2 cut(s) 399, 495
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 5 cut(s) 71, 138, 207, 464, 2274
BslFI GGGAC 4 cut(s) 520, 1142, 2282, 2782
BslI CCNNNNNNNGG 7 cut(s) 71, 77, 124, 364, 406, 1022, 2412
BsmAI GTCTC 4 cut(s) 103, 146, 597, 1450
BsmBI CGTCTC 1 cut(s) 1450
BsmFI GGGAC 4 cut(s) 520, 1142, 2282, 2782
BsmI GAATGC 3 cut(s) 501, 1272, 1424
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 4 cut(s) 399, 460, 495, 2407
Bsp143I GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
Bsp68I TCGCGA 1 cut(s) 474
BspCNI CTCAG 9 cut(s) 107, 257, 383, 652, 1609, 1621, 2081, 2083, 2566
BspFNI CGCG 4 cut(s) 78, 300, 474, 887
BspLI GGNNCC 8 cut(s) 269, 296, 1158, 1297, 1957, 2298, 2321, 2886
BspMAI CTGCAG 2 cut(s) 712, 1422
BspMI ACCTGC 2 cut(s) 850, 1753
BspPI GGATC 5 cut(s) 651, 698, 890, 1290, 1303
BspT107I GGYRCC 2 cut(s) 1955, 2884
BspTI CTTAAG 2 cut(s) 482, 1286
BspTNI GGTCTC 1 cut(s) 146
BsrBI CCGCTC 2 cut(s) 187, 279
BsrDI GCAATG 3 cut(s) 519, 765, 2004
BsrI ACTGG 5 cut(s) 1024, 1072, 1267, 1345, 2127
BssECI CCNNGG 8 cut(s) 23, 70, 76, 624, 1042, 2299, 2407, 2555
BssMI GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
BssSI CACGAG 1 cut(s) 2073
BssT1I CCWWGG 4 cut(s) 23, 624, 1042, 2407
Bst2BI CACGAG 1 cut(s) 2073
Bst2UI CCWGG 2 cut(s) 1002, 2301
Bst4CI ACNGT 7 cut(s) 479, 790, 1385, 2062, 2218, 2761, 2796
Bst6I CTCTTC 3 cut(s) 157, 1596, 2623
BstAFI CTTAAG 2 cut(s) 482, 1286
BstAPI GCANNNNNTGC 1 cut(s) 985
BstC8I GCNNGC 5 cut(s) 325, 847, 1130, 1422, 2467
BstDSI CCRYGG 2 cut(s) 76, 2555
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 6 cut(s) 592, 943, 1252, 1926, 2357, 2719
BstFNI CGCG 4 cut(s) 78, 300, 474, 887
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 8 cut(s) 659, 706, 885, 1298, 2032, 2133, 2197, 2836
BstMAI GTCTC 4 cut(s) 103, 146, 597, 1450
BstMBI GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
BstMCI CGRYCG 1 cut(s) 454
BstMWI GCNNNNNNNGC 8 cut(s) 47, 207, 416, 425, 707, 985, 1822, 1924
BstNI CCWGG 2 cut(s) 1002, 2301
BstNSI RCATGY 1 cut(s) 1000
BstSCI CCNGG 4 cut(s) 69, 70, 1000, 2299
BstSFI CTRYAG 4 cut(s) 708, 1122, 1418, 1518
BstSLI GKGCMC 2 cut(s) 460, 495
BstUI CGCG 4 cut(s) 78, 300, 474, 887
BstV1I GCAGC 9 cut(s) 700, 722, 1190, 1404, 2109, 2120, 2315, 2498, 2690
BstV2I GAAGAC 1 cut(s) 1501
BstX2I RGATCY 2 cut(s) 1295, 2130
BstXI CCANNNNNNTGG 1 cut(s) 24
BstYI RGATCY 2 cut(s) 1295, 2130
BstZI CGGCCG 1 cut(s) 451
BsuI GTATCC 1 cut(s) 778
BtgI CCRYGG 2 cut(s) 76, 2555
BtgZI GCGATG 1 cut(s) 2366
BtrI CACGTC 1 cut(s) 1460
BtsCI GGATG 6 cut(s) 592, 943, 1252, 1926, 2357, 2719
BtsI GCAGTG 2 cut(s) 606, 974
BtsIMutI CAGTG 8 cut(s) 606, 662, 974, 1260, 1903, 2053, 2120, 2223
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 2 cut(s) 850, 1753
Cac8I GCNNGC 5 cut(s) 325, 847, 1130, 1422, 2467
CaiI CAGNNNCTG 2 cut(s) 1007, 2111
CfoI GCGC 1 cut(s) 748
Cfr42I CCGCGG 1 cut(s) 79
Cfr9I CCCGGG 1 cut(s) 70
CseI GACGC 1 cut(s) 343
Csp6I GTAC 6 cut(s) 261, 443, 1956, 2041, 2115, 2757
CviQI GTAC 6 cut(s) 261, 443, 1956, 2041, 2115, 2757
DpnI GATC 8 cut(s) 658, 705, 884, 1297, 2031, 2132, 2196, 2835
DpnII GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
DraIII CACNNNGTG 1 cut(s) 732
EaeI YGGCCR 2 cut(s) 102, 451
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 3 cut(s) 157, 1596, 2623
EarI CTCTTC 3 cut(s) 157, 1596, 2623
EciI GGCGGA 2 cut(s) 134, 203
Ecl136II GAGCTC 1 cut(s) 397
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 4 cut(s) 23, 624, 1042, 2407
Eco147I AGGCCT 1 cut(s) 1524
Eco24I GRGCYC 2 cut(s) 399, 2407
Eco31I GGTCTC 1 cut(s) 146
Eco32I GATATC 1 cut(s) 2785
Eco47I GGWCC 6 cut(s) 1004, 1156, 2296, 2320, 2668, 2737
Eco52I CGGCCG 1 cut(s) 451
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 6 cut(s) 861, 1339, 1420, 1636, 1879, 2029
Eco88I CYCGRG 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 4 cut(s) 267, 294, 1004, 1156
EcoRII CCWGG 2 cut(s) 1000, 2299
EcoRV GATATC 1 cut(s) 2785
EcoT14I CCWWGG 4 cut(s) 23, 624, 1042, 2407
EcoT22I ATGCAT 1 cut(s) 519
EcoT38I GRGCYC 2 cut(s) 399, 2407
ErhI CCWWGG 4 cut(s) 23, 624, 1042, 2407
Esp3I CGTCTC 1 cut(s) 1450
FaqI GGGAC 4 cut(s) 520, 1142, 2282, 2782
FauI CCCGC 4 cut(s) 206, 305, 2772, 2807
FbaI TGATCA 1 cut(s) 2029
FblI GTMKAC 2 cut(s) 2064, 2472
FokI GGATG 6 cut(s) 579, 950, 1259, 1933, 2344, 2726
FriOI GRGCYC 2 cut(s) 399, 2407
FspBI CTAG 9 cut(s) 264, 561, 581, 1430, 1806, 2408, 2421, 2538, 2741
GlaI GCGC 1 cut(s) 747
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 4 cut(s) 1041, 1328, 1847, 2211
HaeII RGCGCY 1 cut(s) 749
HapII CCGG 5 cut(s) 71, 138, 207, 464, 2274
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 748
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HincII GTYRAC 3 cut(s) 1388, 1847, 2271
HindII GTYRAC 3 cut(s) 1388, 1847, 2271
HindIII AAGCTT 1 cut(s) 1563
HpaII CCGG 5 cut(s) 71, 138, 207, 464, 2274
HphI GGTGA 2 cut(s) 2315, 2729
Hpy166II GTNNAC 7 cut(s) 493, 1388, 1847, 1990, 2065, 2271, 2473
Hpy188III TCNNGA 7 cut(s) 473, 537, 550, 641, 965, 1115, 2090
Hpy8I GTNNAC 7 cut(s) 493, 1388, 1847, 1990, 2065, 2271, 2473
Hpy99I CGWCG 5 cut(s) 305, 308, 311, 314, 479
HpyAV CCTTC 5 cut(s) 860, 1650, 1903, 2230, 2444
HpyCH4III ACNGT 7 cut(s) 479, 790, 1385, 2062, 2218, 2761, 2796
HpyCH4IV ACGT 4 cut(s) 468, 1078, 1459, 1934
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 207, 416, 425, 707, 985, 1822, 1924
HpySE526I ACGT 4 cut(s) 468, 1078, 1459, 1934
HspAI GCGC 1 cut(s) 746
KpnI GGTACC 1 cut(s) 1959
Ksp22I TGATCA 1 cut(s) 2029
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
LmnI GCTCC 4 cut(s) 279, 284, 351, 402
Lsp1109I GCAGC 9 cut(s) 700, 722, 1190, 1404, 2109, 2120, 2315, 2498, 2690
LweI GCATC 8 cut(s) 406, 688, 1430, 1592, 1812, 1984, 2366, 2814
MaeI CTAG 9 cut(s) 264, 561, 581, 1430, 1806, 2408, 2421, 2538, 2741
MaeII ACGT 4 cut(s) 468, 1078, 1459, 1934
MaeIII GTNAC 7 cut(s) 167, 722, 1253, 1361, 1808, 2018, 2648
MalI GATC 8 cut(s) 658, 705, 884, 1297, 2031, 2132, 2196, 2835
MbiI CCGCTC 2 cut(s) 187, 279
MboI GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
MboII GAAGA 7 cut(s) 144, 867, 1204, 1506, 1583, 1742, 2610
MfeI CAATTG 4 cut(s) 693, 1578, 1782, 2291
MflI RGATCY 2 cut(s) 1295, 2130
MhlI GDGCHC 4 cut(s) 399, 460, 495, 2407
MlyI GAGTC 2 cut(s) 362, 2823
MmeI TCCRAC 5 cut(s) 252, 550, 1040, 1323, 2674
Mph1103I ATGCAT 1 cut(s) 519
MseI TTAA 9 cut(s) 483, 1287, 1662, 1914, 1938, 2445, 2772, 2864, 2926
MslI CAYNNNNRTG 5 cut(s) 597, 611, 1265, 2025, 2031
MspA1I CMGCKG 4 cut(s) 78, 713, 1359, 2111
MspCI CTTAAG 2 cut(s) 482, 1286
MspI CCGG 5 cut(s) 71, 138, 207, 464, 2274
MspR9I CCNGG 4 cut(s) 71, 72, 1002, 2301
MunI CAATTG 4 cut(s) 693, 1578, 1782, 2291
Mva1269I GAATGC 3 cut(s) 501, 1272, 1424
MvaI CCWGG 2 cut(s) 1002, 2301
MvnI CGCG 4 cut(s) 78, 300, 474, 887
MwoI GCNNNNNNNGC 8 cut(s) 47, 207, 416, 425, 707, 985, 1822, 1924
NciI CCSGG 2 cut(s) 71, 72
NdeII GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
NlaIV GGNNCC 8 cut(s) 269, 296, 1158, 1297, 1957, 2298, 2321, 2886
NmeAIII GCCGAG 2 cut(s) 80, 429
NmuCI GTSAC 3 cut(s) 1253, 1808, 2018
NruI TCGCGA 1 cut(s) 474
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 1000
OliI CACNNNNGTG 1 cut(s) 611
PaqCI CACCTGC 1 cut(s) 1753
PceI AGGCCT 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 327, 381
PctI GAATGC 3 cut(s) 501, 1272, 1424
PleI GAGTC 2 cut(s) 362, 2823
PpsI GAGTC 2 cut(s) 362, 2823
PpuMI RGGWCCY 2 cut(s) 1004, 1156
PshBI ATTAAT 1 cut(s) 2864
Psp124BI GAGCTC 1 cut(s) 399
Psp5II RGGWCCY 2 cut(s) 1004, 1156
Psp6I CCWGG 2 cut(s) 1000, 2299
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 1000, 2299
PspN4I GGNNCC 8 cut(s) 269, 296, 1158, 1297, 1957, 2298, 2321, 2886
PspPPI RGGWCCY 2 cut(s) 1004, 1156
PstI CTGCAG 2 cut(s) 712, 1422
PstNI CAGNNNCTG 2 cut(s) 1007, 2111
PsuI RGATCY 2 cut(s) 1295, 2130
PvuII CAGCTG 3 cut(s) 713, 1359, 2111
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 6 cut(s) 262, 444, 1957, 2042, 2116, 2758
RsaNI GTAC 6 cut(s) 261, 443, 1956, 2041, 2115, 2757
RseI CAYNNNNRTG 5 cut(s) 597, 611, 1265, 2025, 2031
SacI GAGCTC 1 cut(s) 399
SacII CCGCGG 1 cut(s) 79
SaqAI TTAA 9 cut(s) 483, 1287, 1662, 1914, 1938, 2445, 2772, 2864, 2926
Sau3AI GATC 8 cut(s) 656, 703, 882, 1295, 2029, 2130, 2194, 2833
SchI GAGTC 2 cut(s) 362, 2823
ScrFI CCNGG 4 cut(s) 71, 72, 1002, 2301
SduI GDGCHC 4 cut(s) 399, 460, 495, 2407
SfaNI GCATC 8 cut(s) 406, 688, 1430, 1592, 1812, 1984, 2366, 2814
SfcI CTRYAG 4 cut(s) 708, 1122, 1418, 1518
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 6 cut(s) 1004, 1156, 2296, 2320, 2668, 2737
SmaI CCCGGG 1 cut(s) 72
SmiMI CAYNNNNRTG 5 cut(s) 597, 611, 1265, 2025, 2031
SmlI CTYRAG 3 cut(s) 482, 1286, 2602
SmoI CTYRAG 3 cut(s) 482, 1286, 2602
SseBI AGGCCT 1 cut(s) 1524
SspI AATATT 1 cut(s) 1638
SspMI CTAG 9 cut(s) 264, 561, 581, 1430, 1806, 2408, 2421, 2538, 2741
SstI GAGCTC 1 cut(s) 399
StuI AGGCCT 1 cut(s) 1524
StyD4I CCNGG 4 cut(s) 69, 70, 1000, 2299
StyI CCWWGG 4 cut(s) 23, 624, 1042, 2407
TaaI ACNGT 7 cut(s) 479, 790, 1385, 2062, 2218, 2761, 2796
TaiI ACGT 4 cut(s) 471, 1081, 1462, 1937
TaqI TCGA 5 cut(s) 1063, 1820, 1951, 2401, 2832
TaqII GACCGA 2 cut(s) 767, 2685
TatI WGTACW 2 cut(s) 442, 2040
TauI GCSGC 5 cut(s) 78, 81, 330, 501, 2103
Tru1I TTAA 9 cut(s) 483, 1287, 1662, 1914, 1938, 2445, 2772, 2864, 2926
Tru9I TTAA 9 cut(s) 483, 1287, 1662, 1914, 1938, 2445, 2772, 2864, 2926
TscAI CASTG 8 cut(s) 613, 669, 981, 1267, 1903, 2053, 2127, 2223
TseFI GTSAC 3 cut(s) 1253, 1808, 2018
TseI GCWGC 9 cut(s) 710, 713, 1178, 1417, 2097, 2108, 2328, 2486, 2678
Tsp45I GTSAC 3 cut(s) 1253, 1808, 2018
TspGWI ACGGA 4 cut(s) 155, 224, 2312, 2382
TspMI CCCGGG 1 cut(s) 70
TspRI CASTG 8 cut(s) 613, 669, 981, 1267, 1903, 2053, 2127, 2223
Vha464I CTTAAG 2 cut(s) 482, 1286
VneI GTGCAC 1 cut(s) 491
VpaK11BI GGWCC 6 cut(s) 1004, 1156, 2296, 2320, 2668, 2737
VspI ATTAAT 1 cut(s) 2864
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 6 cut(s) 587, 682, 856, 1466, 1716, 2085
XceI RCATGY 1 cut(s) 1000
XmaI CCCGGG 1 cut(s) 70
XmaJI CCTAGG 1 cut(s) 2407
XmiI GTMKAC 2 cut(s) 2064, 2472
XspI CTAG 9 cut(s) 264, 561, 581, 1430, 1806, 2408, 2421, 2538, 2741
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.