Rh1AG117100

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0010284
Physical Location & Seq
Forward (+)
20118227 .. 20132253
14027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG117100.1

Sequence Viewer

Length: 2856 bp
ATGAAATCCTCAGTCTCGGCCATAACCCCAACCCCAACCCCAACCCCCAACATGGCCTTAGCCTCCGGTCTCCGCCTCTCTTCCTCTATCCTCCGTTACCGCCTCCCTGCACCGCTCAACTCCACCCGCTTCTGCGGCCTCCGCCACAGCCTCTCCGACCTCCGTTTCCTCTCAGCTTCCTCCTCTCGTACTAGGCCCCAGCTCCGCTCCATCAAAGCGAGGCCCCGCGACGACGACGACGACCTCCTCGCAGGCGGCGAGGGGCAGAATGGCAATGGGAGCGTCCTTGTCAAGGACTCAGACGAGAGAATCGTTCGTGTTGAGCTCCACAAGGAGGCCACCGATGCCTACATGGCCTATGCTATGTCTGTACTGCTCGGCCGTGCCCTGCCGGACGTTCGCGACGGTCTTAAGCCAGTGCACCGCCGCATTCTGTATGCAATGCATGAATTGAGGCTTTCGTCCCGAAAACCATTCGTGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAGTTTCATCCTCATGGAGACACTGCTGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCTTTACGATCCCCACTGATTCAAGGGCATGGGAATTTTGGTTCAATTGATGCTGATCCTGCAGCTGCCATGCGTTACACAGAGTGCAGATTGGAGGCTCTGACCGAAGCAATGTTGCTGTCAGATTTAGAACAGGATACGGTTGATTTTGTTCCAAACTTTGATAATTCACAAAAAGAGCCATCACTTTTACCTGCTCGCCTTCCAAATTTATTATTGAATGGTTCTTCAGGGATTGCGGTTGGCATGGCAACAAATATCCCTCCGCATAATCTTGGGGAATTGGTGGATGTTCTTAGTGTGTTGATTCATAATCCAGAAGCTACACTGCAAGAATTGCTGGAATACATGCCAGGACCTGATTTTCCAACTGGAGGAGTGATTATGGGCAACCTTGGTATCTTAGAGGCATATCGAACTGGGAGAGGACGTATTGTGGTCAGAGGAAAAACTGATATAGAATCTCCTGATTCTAAAGGCAAGCGAAGTGCAATCATCATAAAGGAGGTCCCTTACCAAACTAACAAAGCAGCTCTGGTGGAGAAGATTGCACAACTTGTAGAGGATAAGATTTTAGATGGCATTAGTGATATTAGGGATGAGAGTGACCGCACTGGAATGCGTGTAGTGATTGAGCTCAAGCGAGGATCCGACCCATCTATTGTTCTGAATAATCTATATCGCCTCACATCTCTCCAGTCCAGCTTCAGCTGTAACATGGTTGGTATTCTCAACGGTCAACCTAAACTGATGGGTCTAAAGGAATTGCTGCAGGCATTCCTAGACTTCAGATGCTCTGTTATTGAGAGACGTGCTAAATTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGTATTGCTGTAGGCCTTGATAATTTGCAAAGAGTAATGCGTATAAGTTTGGAAGCTTCTAATAATGCAATTGCATCATCTCTTCTAAGAAATGAGTTCAACCTTTCTGAGAAACAAGCTGAGGCAATATTAGACTTCAACTTCAGAAGGCTTAATGTTATGGAGAGGAAAAAGTTTGATAATGAAAGTGAATCGTTGAAAGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAAAAACATATATTGCAGGTGGTTGAACAAGAAGCAATTGACATAAAAACCAAGTTTGCTAGTCACAGGCGTTCGATGCTGGAGGAAACTGATGGTGGTCAACTTGATGATATTGATGTTATTCCAAATGATGAAATGCTTTTGGCCTTCAGTGAAAAGGGGTATGTTAAGCGGATGAAGCCCAACACGTTTAATTTGCAAAATCGAGGTACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCACGCACATGATCATGTCTTGTACTTCAGTGATAAAGGAACAGTCTACTCAGCTCGTGCATATAAAATTCCTGAGTGCAGCCGCACAGCAGCTGGTACGCCACTGGTTCAGATCTTATCTCTGTCAGATGGCGAGAGAATTACATCTATTATTCCTGTGAGTGAGTTTGCTGGAGATCAGTTTTTACTAATGCTTACAGTGAATGGCTACATAAAGAAGGTGTCTTTGAGTTCTTTTTCATCAATCAGGTCGACCGGAATCATAGCAATTCAACTGGTCCCTGGTGATGAGCTGAAATGGGTCCGTTGCTGCACAAATGATGACCTTGTAGCGATGGCATCCCTAAACGGAATGGTCATCTTATGTTCGTCTGATATTATTCGAGCCCTAGGCAGGAACACTCGGGGTTCTGTAGCCATGCGACTTAAAGAAGGCGATAAGATGGCAAGCGTAGATATTATACCAGCAGCTATGCGGAAAGACTTGGAGAGGGTGTCAGAAGCACCAGAAAGCACGGCTAGAAGTCTGAAAGGCCCGTGGTTGTTGTTTGTGTCTGAGAATGGATATGGAAAGCGTGTTCCCTTGAGCAGATTTCACTCTTCCCGTCTGAACAGAGTGGGTCTGATAGGTTACAAGTTTTCTGCAGAGGACCGATTGGCAGCAGTTTTTGTGGTTGGATTCTCTGTGGCAGAGGATGGTGAAAGTGATGAACAAGTGGTCCTAGTAAGCCAAAGTGGTACTGTGAATAGAATTAAGGTGCGGGATATCTCAATACAGTCTCGCTATGCAAGGGGTGTTATTTTGATGCGACTCGATCTTGCTGGAAAGATTCAGTCTGCTTCATTAATATCAGCAACAGATGAGGTGCCAGAGATTGAAGTTGATGATGAAGCTGCAATTCATGGTTGA

Protein Analysis

951

Amino Acids

104.88

Weight (kDa)

7.6

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 128 - 569 4e-156 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 603 - 649 2.5e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 653 - 703 5.7e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 710 - 753 3.9e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 757 - 803 4e-07 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 829 - 874 9.3e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 887 - 932 2.7e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1681
Acc36I ACCTGC 2 cut(s) 778, 1681
Acc65I GGTACC 1 cut(s) 1883
AccB1I GGYRCC 2 cut(s) 1883, 2812
AccBSI CCGCTC 2 cut(s) 115, 207
AccI GTMKAC 2 cut(s) 1992, 2198
AccII CGCG 2 cut(s) 228, 402
AclWI GGATC 4 cut(s) 579, 626, 1218, 1231
AcoI YGGCCR 2 cut(s) 18, 379
AcsI RAATTY 5 cut(s) 610, 784, 1394, 1644, 2013
AcuI CTGAAG 6 cut(s) 789, 1267, 1348, 1564, 1807, 1957
AdeI CACNNNGTG 1 cut(s) 660
AfaI GTAC 6 cut(s) 190, 372, 1885, 1970, 2044, 2686
AfiI CCNNNNNNNGG 5 cut(s) 52, 292, 334, 950, 2340
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 1860
AjiI CACGTC 1 cut(s) 1388
AjnI CCWGG 2 cut(s) 928, 2227
AleI CACNNNNGTG 1 cut(s) 539
AloI GAACNNNNNNTCC 6 cut(s) 601, 633, 2508, 2540, 2649, 2681
Alw21I GWGCWC 3 cut(s) 327, 423, 1215
Alw26I GTCTC 5 cut(s) 19, 74, 525, 1378, 2730
Alw44I GTGCAC 1 cut(s) 419
AlwI GGATC 4 cut(s) 579, 626, 1218, 1231
AlwNI CAGNNNCTG 2 cut(s) 935, 2039
Ama87I CYCGRG 1 cut(s) 2349
ApaLI GTGCAC 1 cut(s) 419
ApoI RAATTY 5 cut(s) 610, 784, 1394, 1644, 2013
AseI ATTAAT 1 cut(s) 2792
Asp718I GGTACC 1 cut(s) 1883
AspA2I CCTAGG 1 cut(s) 2335
AspS9I GGNCC 9 cut(s) 195, 222, 932, 1084, 2224, 2248, 2480, 2596, 2665
AsuHPI GGTGA 2 cut(s) 2243, 2657
AvaI CYCGRG 1 cut(s) 2349
AvaII GGWCC 6 cut(s) 932, 1084, 2224, 2248, 2596, 2665
AvrII CCTAGG 1 cut(s) 2335
BaeGI GKGCMC 2 cut(s) 388, 423
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BamHI GGATCC 1 cut(s) 1223
BanI GGYRCC 2 cut(s) 1883, 2812
BanII GRGCYC 3 cut(s) 327, 1215, 2335
BauI CACGAG 1 cut(s) 2001
BbsI GAAGAC 1 cut(s) 1429
Bbv12I GWGCWC 3 cut(s) 327, 423, 1215
BbvCI CCTCAGC 1 cut(s) 1557
BceAI ACGGC 2 cut(s) 366, 2478
BciT130I CCWGG 2 cut(s) 930, 2229
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 1 cut(s) 1957
BcoDI GTCTC 5 cut(s) 19, 74, 525, 1378, 2730
BfaI CTAG 8 cut(s) 192, 489, 509, 1358, 1734, 2336, 2466, 2669
BfmI CTRYAG 5 cut(s) 636, 1346, 1446, 2358, 2589
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 2 cut(s) 778, 1681
BfuI GTATCC 1 cut(s) 706
BglI GCCNNNNNGGC 1 cut(s) 353
BglII AGATCT 1 cut(s) 2058
BlnI CCTAGG 1 cut(s) 2335
Bme1390I CCNGG 2 cut(s) 930, 2229
Bme18I GGWCC 6 cut(s) 932, 1084, 2224, 2248, 2596, 2665
BmeT110I CYCGRG 1 cut(s) 2349
BmgBI CACGTC 1 cut(s) 1388
BmgT120I GGNCC 9 cut(s) 195, 222, 932, 1084, 2224, 2248, 2480, 2596, 2665
BmiI GGNNCC 8 cut(s) 197, 224, 1086, 1225, 1885, 2226, 2249, 2814
BmrFI CCNGG 2 cut(s) 930, 2229
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 8 cut(s) 334, 616, 1358, 1520, 1740, 1912, 2294, 2742
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 1 cut(s) 1429
BpmI CTGGAG 4 cut(s) 969, 1256, 1775, 2139
Bpu10I CCTNAGC 3 cut(s) 58, 567, 1557
BpuEI CTTGAG 2 cut(s) 1199, 2551
BsaBI GATNNNNATC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 552, 970, 2227, 2335, 2483
BsaWI WCCGGW 2 cut(s) 65, 2201
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 991, 1021, 2426, 2456
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 292, 334, 950, 2340
Bse1I ACTGG 7 cut(s) 416, 952, 1000, 1195, 1273, 2055, 2226
Bse3DI GCAATG 4 cut(s) 280, 447, 693, 1932
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 2 cut(s) 930, 2229
BseDI CCNNGG 5 cut(s) 552, 970, 2227, 2335, 2483
BseGI GGATG 6 cut(s) 520, 871, 1180, 1854, 2285, 2647
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 5 cut(s) 52, 292, 334, 950, 2340
BseMI GCAATG 4 cut(s) 280, 447, 693, 1932
BseMII CTCAG 9 cut(s) 24, 186, 312, 581, 1536, 1548, 2010, 2010, 2493
BseNI ACTGG 7 cut(s) 416, 952, 1000, 1195, 1273, 2055, 2226
BseRI GAGGAG 3 cut(s) 172, 236, 966
BseSI GKGCMC 2 cut(s) 388, 423
BseX3I CGGCCG 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 4 cut(s) 93, 682, 2044, 2242
Bsh1236I CGCG 2 cut(s) 228, 402
Bsh1285I CGRYCG 2 cut(s) 382, 2202
BshNI GGYRCC 2 cut(s) 1883, 2812
BsiEI CGRYCG 2 cut(s) 382, 2202
BsiHKAI GWGCWC 3 cut(s) 327, 423, 1215
BsiHKCI CYCGRG 1 cut(s) 2349
BsiSI CCGG 3 cut(s) 66, 392, 2202
BslFI GGGAC 3 cut(s) 448, 1070, 2210
BslI CCNNNNNNNGG 5 cut(s) 52, 292, 334, 950, 2340
BsmAI GTCTC 5 cut(s) 19, 74, 525, 1378, 2730
BsmBI CGTCTC 1 cut(s) 1378
BsmFI GGGAC 3 cut(s) 448, 1070, 2210
BsmI GAATGC 3 cut(s) 429, 1200, 1352
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 2349
Bsp1286I GDGCHC 5 cut(s) 327, 388, 423, 1215, 2335
Bsp143I GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
Bsp68I TCGCGA 1 cut(s) 402
BspCNI CTCAG 9 cut(s) 23, 185, 311, 580, 1537, 1549, 2009, 2011, 2494
BspFNI CGCG 2 cut(s) 228, 402
BspLI GGNNCC 8 cut(s) 197, 224, 1086, 1225, 1885, 2226, 2249, 2814
BspMAI CTGCAG 3 cut(s) 640, 1350, 2593
BspMI ACCTGC 2 cut(s) 778, 1681
BspPI GGATC 4 cut(s) 579, 626, 1218, 1231
BspT107I GGYRCC 2 cut(s) 1883, 2812
BspTI CTTAAG 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 74
BsrBI CCGCTC 2 cut(s) 115, 207
BsrDI GCAATG 4 cut(s) 280, 447, 693, 1932
BsrI ACTGG 7 cut(s) 416, 952, 1000, 1195, 1273, 2055, 2226
BssECI CCNNGG 5 cut(s) 552, 970, 2227, 2335, 2483
BssMI GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
BssSI CACGAG 1 cut(s) 2001
BssT1I CCWWGG 3 cut(s) 552, 970, 2335
Bst2BI CACGAG 1 cut(s) 2001
Bst2UI CCWGG 2 cut(s) 930, 2229
Bst4CI ACNGT 7 cut(s) 407, 718, 1313, 1990, 2146, 2689, 2724
Bst6I CTCTTC 3 cut(s) 85, 1524, 2551
BstAFI CTTAAG 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 913
BstC8I GCNNGC 5 cut(s) 253, 775, 1058, 1350, 2395
BstDSI CCRYGG 1 cut(s) 2483
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 6 cut(s) 520, 871, 1180, 1854, 2285, 2647
BstFNI CGCG 2 cut(s) 228, 402
BstKTI GATC 7 cut(s) 587, 634, 1226, 1960, 2061, 2125, 2764
BstMAI GTCTC 5 cut(s) 19, 74, 525, 1378, 2730
BstMBI GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
BstMCI CGRYCG 2 cut(s) 382, 2202
BstMWI GCNNNNNNNGC 9 cut(s) 135, 141, 279, 344, 353, 635, 913, 1750, 1852
BstNI CCWGG 2 cut(s) 930, 2229
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 2 cut(s) 928, 2227
BstSFI CTRYAG 5 cut(s) 636, 1346, 1446, 2358, 2589
BstSLI GKGCMC 2 cut(s) 388, 423
BstUI CGCG 2 cut(s) 228, 402
BstV2I GAAGAC 1 cut(s) 1429
BstX2I RGATCY 2 cut(s) 1223, 2058
BstYI RGATCY 2 cut(s) 1223, 2058
BstZI CGGCCG 1 cut(s) 379
BsuI GTATCC 1 cut(s) 706
BtgI CCRYGG 1 cut(s) 2483
BtgZI GCGATG 1 cut(s) 2294
BtrI CACGTC 1 cut(s) 1388
BtsCI GGATG 6 cut(s) 520, 871, 1180, 1854, 2285, 2647
BtsI GCAGTG 2 cut(s) 534, 902
BtsIMutI CAGTG 9 cut(s) 423, 534, 590, 902, 1188, 1831, 1981, 2048, 2151
BtuMI TCGCGA 1 cut(s) 402
BveI ACCTGC 2 cut(s) 778, 1681
Cac8I GCNNGC 5 cut(s) 253, 775, 1058, 1350, 2395
CaiI CAGNNNCTG 2 cut(s) 935, 2039
Cfr13I GGNCC 9 cut(s) 195, 222, 932, 1084, 2224, 2248, 2480, 2596, 2665
CseI GACGC 1 cut(s) 271
Csp6I GTAC 6 cut(s) 189, 371, 1884, 1969, 2043, 2685
CviQI GTAC 6 cut(s) 189, 371, 1884, 1969, 2043, 2685
DpnI GATC 7 cut(s) 586, 633, 1225, 1959, 2060, 2124, 2763
DpnII GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
DraIII CACNNNGTG 1 cut(s) 660
EaeI YGGCCR 2 cut(s) 18, 379
EagI CGGCCG 1 cut(s) 379
Eam1104I CTCTTC 3 cut(s) 85, 1524, 2551
EarI CTCTTC 3 cut(s) 85, 1524, 2551
EciI GGCGGA 2 cut(s) 62, 131
Ecl136II GAGCTC 2 cut(s) 325, 1213
EclXI CGGCCG 1 cut(s) 379
Eco130I CCWWGG 3 cut(s) 552, 970, 2335
Eco147I AGGCCT 1 cut(s) 1452
Eco24I GRGCYC 3 cut(s) 327, 1215, 2335
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 2713
Eco47I GGWCC 6 cut(s) 932, 1084, 2224, 2248, 2596, 2665
Eco52I CGGCCG 1 cut(s) 379
Eco53kI GAGCTC 2 cut(s) 325, 1213
Eco57I CTGAAG 6 cut(s) 789, 1267, 1348, 1564, 1807, 1957
Eco88I CYCGRG 1 cut(s) 2349
EcoICRI GAGCTC 2 cut(s) 325, 1213
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO109I RGGNCCY 4 cut(s) 195, 222, 932, 1084
EcoRII CCWGG 2 cut(s) 928, 2227
EcoRV GATATC 1 cut(s) 2713
EcoT14I CCWWGG 3 cut(s) 552, 970, 2335
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 3 cut(s) 327, 1215, 2335
ErhI CCWWGG 3 cut(s) 552, 970, 2335
Esp3I CGTCTC 1 cut(s) 1378
FaqI GGGAC 3 cut(s) 448, 1070, 2210
FauI CCCGC 3 cut(s) 134, 233, 2700
FbaI TGATCA 1 cut(s) 1957
FblI GTMKAC 2 cut(s) 1992, 2198
FokI GGATG 6 cut(s) 507, 878, 1187, 1861, 2272, 2654
FriOI GRGCYC 3 cut(s) 327, 1215, 2335
FspBI CTAG 8 cut(s) 192, 489, 509, 1358, 1734, 2336, 2466, 2669
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 4 cut(s) 969, 1256, 1775, 2139
HapII CCGG 3 cut(s) 66, 392, 2202
HgaI GACGC 1 cut(s) 271
HincII GTYRAC 3 cut(s) 1316, 1775, 2199
HindII GTYRAC 3 cut(s) 1316, 1775, 2199
HindIII AAGCTT 1 cut(s) 1491
HpaII CCGG 3 cut(s) 66, 392, 2202
HphI GGTGA 2 cut(s) 2243, 2657
Hpy166II GTNNAC 6 cut(s) 421, 1316, 1775, 1918, 1993, 2199
Hpy188III TCNNGA 7 cut(s) 401, 465, 478, 569, 893, 1043, 2018
Hpy8I GTNNAC 6 cut(s) 421, 1316, 1775, 1918, 1993, 2199
Hpy99I CGWCG 5 cut(s) 233, 236, 239, 242, 407
HpyAV CCTTC 5 cut(s) 788, 1578, 1831, 2158, 2372
HpyCH4III ACNGT 7 cut(s) 407, 718, 1313, 1990, 2146, 2689, 2724
HpyCH4IV ACGT 4 cut(s) 396, 1006, 1387, 1862
HpyF10VI GCNNNNNNNGC 9 cut(s) 135, 141, 279, 344, 353, 635, 913, 1750, 1852
HpySE526I ACGT 4 cut(s) 396, 1006, 1387, 1862
KpnI GGTACC 1 cut(s) 1887
Ksp22I TGATCA 1 cut(s) 1957
Kzo9I GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
LmnI GCTCC 4 cut(s) 207, 212, 279, 330
LweI GCATC 8 cut(s) 334, 616, 1358, 1520, 1740, 1912, 2294, 2742
MaeI CTAG 8 cut(s) 192, 489, 509, 1358, 1734, 2336, 2466, 2669
MaeII ACGT 4 cut(s) 396, 1006, 1387, 1862
MaeIII GTNAC 7 cut(s) 95, 650, 1181, 1289, 1736, 1946, 2576
MalI GATC 7 cut(s) 586, 633, 1225, 1959, 2060, 2124, 2763
MbiI CCGCTC 2 cut(s) 115, 207
MboI GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
MboII GAAGA 7 cut(s) 72, 795, 1132, 1434, 1511, 1670, 2538
MfeI CAATTG 3 cut(s) 621, 1506, 1710
MflI RGATCY 2 cut(s) 1223, 2058
MhlI GDGCHC 5 cut(s) 327, 388, 423, 1215, 2335
MlyI GAGTC 2 cut(s) 290, 2751
MmeI TCCRAC 5 cut(s) 180, 478, 968, 1251, 2602
Mph1103I ATGCAT 1 cut(s) 447
MseI TTAA 7 cut(s) 411, 1590, 1842, 1866, 2373, 2700, 2792
MslI CAYNNNNRTG 5 cut(s) 525, 539, 1193, 1953, 1959
MspA1I CMGCKG 3 cut(s) 641, 1287, 2039
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 3 cut(s) 66, 392, 2202
MspR9I CCNGG 2 cut(s) 930, 2229
MunI CAATTG 3 cut(s) 621, 1506, 1710
Mva1269I GAATGC 3 cut(s) 429, 1200, 1352
MvaI CCWGG 2 cut(s) 930, 2229
MvnI CGCG 2 cut(s) 228, 402
MwoI GCNNNNNNNGC 9 cut(s) 135, 141, 279, 344, 353, 635, 913, 1750, 1852
NdeII GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
NlaIV GGNNCC 8 cut(s) 197, 224, 1086, 1225, 1885, 2226, 2249, 2814
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 3 cut(s) 1181, 1736, 1946
NruI TCGCGA 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 1 cut(s) 928
OliI CACNNNNGTG 1 cut(s) 539
PaqCI CACCTGC 1 cut(s) 1681
PceI AGGCCT 1 cut(s) 1452
PcsI WCGNNNNNNNCGW 3 cut(s) 237, 255, 309
PctI GAATGC 3 cut(s) 429, 1200, 1352
PleI GAGTC 2 cut(s) 290, 2751
PpsI GAGTC 2 cut(s) 290, 2751
PpuMI RGGWCCY 2 cut(s) 932, 1084
PshBI ATTAAT 1 cut(s) 2792
Psp124BI GAGCTC 2 cut(s) 327, 1215
Psp5II RGGWCCY 2 cut(s) 932, 1084
Psp6I CCWGG 2 cut(s) 928, 2227
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 2 cut(s) 928, 2227
PspN4I GGNNCC 8 cut(s) 197, 224, 1086, 1225, 1885, 2226, 2249, 2814
PspPI GGNCC 9 cut(s) 195, 222, 932, 1084, 2224, 2248, 2480, 2596, 2665
PspPPI RGGWCCY 2 cut(s) 932, 1084
PstI CTGCAG 3 cut(s) 640, 1350, 2593
PstNI CAGNNNCTG 2 cut(s) 935, 2039
PsuI RGATCY 2 cut(s) 1223, 2058
PvuII CAGCTG 3 cut(s) 641, 1287, 2039
RruI TCGCGA 1 cut(s) 402
RsaI GTAC 6 cut(s) 190, 372, 1885, 1970, 2044, 2686
RsaNI GTAC 6 cut(s) 189, 371, 1884, 1969, 2043, 2685
RseI CAYNNNNRTG 5 cut(s) 525, 539, 1193, 1953, 1959
SacI GAGCTC 2 cut(s) 327, 1215
SalI GTCGAC 1 cut(s) 2197
SaqAI TTAA 7 cut(s) 411, 1590, 1842, 1866, 2373, 2700, 2792
Sau3AI GATC 7 cut(s) 584, 631, 1223, 1957, 2058, 2122, 2761
Sau96I GGNCC 9 cut(s) 195, 222, 932, 1084, 2224, 2248, 2480, 2596, 2665
SchI GAGTC 2 cut(s) 290, 2751
ScrFI CCNGG 2 cut(s) 930, 2229
SduI GDGCHC 5 cut(s) 327, 388, 423, 1215, 2335
SfaNI GCATC 8 cut(s) 334, 616, 1358, 1520, 1740, 1912, 2294, 2742
SfcI CTRYAG 5 cut(s) 636, 1346, 1446, 2358, 2589
SinI GGWCC 6 cut(s) 932, 1084, 2224, 2248, 2596, 2665
SmiMI CAYNNNNRTG 5 cut(s) 525, 539, 1193, 1953, 1959
SmlI CTYRAG 3 cut(s) 410, 1214, 2530
SmoI CTYRAG 3 cut(s) 410, 1214, 2530
SseBI AGGCCT 1 cut(s) 1452
SspI AATATT 1 cut(s) 1566
SspMI CTAG 8 cut(s) 192, 489, 509, 1358, 1734, 2336, 2466, 2669
SstI GAGCTC 2 cut(s) 327, 1215
StuI AGGCCT 1 cut(s) 1452
StyD4I CCNGG 2 cut(s) 928, 2227
StyI CCWWGG 3 cut(s) 552, 970, 2335
TaaI ACNGT 7 cut(s) 407, 718, 1313, 1990, 2146, 2689, 2724
TaiI ACGT 4 cut(s) 399, 1009, 1390, 1865
TaqI TCGA 6 cut(s) 991, 1748, 1879, 2198, 2329, 2760
TaqII GACCGA 2 cut(s) 695, 2613
TatI WGTACW 2 cut(s) 370, 1968
TauI GCSGC 4 cut(s) 138, 258, 429, 2031
Tru1I TTAA 7 cut(s) 411, 1590, 1842, 1866, 2373, 2700, 2792
Tru9I TTAA 7 cut(s) 411, 1590, 1842, 1866, 2373, 2700, 2792
TscAI CASTG 9 cut(s) 423, 541, 597, 909, 1195, 1831, 1981, 2055, 2151
TseFI GTSAC 3 cut(s) 1181, 1736, 1946
Tsp45I GTSAC 3 cut(s) 1181, 1736, 1946
TspGWI ACGGA 4 cut(s) 83, 152, 2240, 2310
TspRI CASTG 9 cut(s) 423, 541, 597, 909, 1195, 1831, 1981, 2055, 2151
Vha464I CTTAAG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 419
VpaK11BI GGWCC 6 cut(s) 932, 1084, 2224, 2248, 2596, 2665
VspI ATTAAT 1 cut(s) 2792
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 5 cut(s) 610, 784, 1394, 1644, 2013
XceI RCATGY 1 cut(s) 928
XmaJI CCTAGG 1 cut(s) 2335
XmiI GTMKAC 2 cut(s) 1992, 2198
XspI CTAG 8 cut(s) 192, 489, 509, 1358, 1734, 2336, 2466, 2669
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.