MD02G1265100.v1.1

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010284
Physical Location & Seq
Reverse (-)
31921207 .. 31936520
15314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1265100.v1.1.491

Sequence Viewer

Length: 2835 bp
ATGAAACCCTCAGTCTCCCTCCACCCTCCTACCATGGCCCTAGCTTCCAGTCTCCGCTTGTCTTCCTCCATCCTCCGCTACCGCCTCGCCGCCCCGCTCAACCCGACCCGACTCTCCGGTCTCCGCCACAACCTCTCGGAGCTCCGCTTCCTTTCAGCATCATCCTCCCGCACCAGGACCCGCGTCCGCCCAATTAAAGCGAGGCTCCTTGACGAACCCCAAAAGGAGGACCCCGGTGAGGCGCAAGATGGGAACGGGAGTGTGCTGGTCAAGGACACAAGCGAGAATAGCGAAAGAATTGTCCGCGTTGAGCTCCACAAGGAGGCCACTGAAGCCTACATGGCATACGCTATGTCTGTCCTGCTCGGCCGCGCCCTGCCCGATGTTCGTGACGGTTTGAAGCCCGTGCACCGCCGGATTTTGTATGCAATGCACGAATTAGGGATTGTATCGCGGAAACCGTTCAAGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTCCATCCCCATGGAGACACTGCCGTGTATGATTCCTTGGTGCGAATGGCTCAGGATTTCTCCTTACGATTCCCACTAATCCAAGGGCATGGGAATTTTGGTTCTATTGATGCTGATCCTGCAGCTGCTATGCGTTACACGGAATGCAGATTGGAAGCACTGACAGAAGCAATGCTGTTGGCAGATTTAGATCAAAATACGGTTGATTTTACTCCAAATTTTGATAATTCACAAAAAGAGCCATCAGTTTTACCTGCTCGACTTCCAACTTTATTACTGAATGGTGCTTCTGGGATTGCGGTTGGCATGGCAACCAACATTCCTCCACATAATCTTGGGGAATTGGTAGATGTTCTTAGCGTGTTAATTCACAATCCAGAAGCTACGTTGCAAGAATTACTAGAATACATGCCAGGACCAGATTTTCCAACTGGAGGACTGATTATGGGCAACCTTGGAATCTTGGACGCATATCGAACTGGAAAAGGACGTATTGTTGTAAGAGGGAAAACTGATGTTGAATTGCTTGATTCTAAAACAAAGAGAAATGCAATTATCATAAAAGAGATCCCTTACCAAACCAACAAAGCAGCTCTTGTGGAGAAGATAGCTGAACTTGTGGAAAATAAGATTTTGGAAGGCATAAGTGATATTAGGGATGAAAGTGATCGTTCTGGAATGCGTGTGGTCATTGAGCTTAAGCGAGGATCTGACCCATCAATTGTTCTAAATAATCTCTATCGCCTCACATCTCTTCAATGCAGTTTTAGTTGTAACATGGTGGGTATTCATAATGGGCAACCTAAGCAGATGGGTCTAAAGGAATTGCTGCAGGCATTCTTAGACTTCAGATGCTCTGTTGTCGAGAGACGAGCTAAGTTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGCATTGTTGTGGGCCTTGATAATTTGGATTCAGTAATTCGTATACTAAGGGAAGCTTCAAGCAATGCAGTTGCATCAGCTGGTTTAAGGACTGAGTTCAGTCTTTCTGAGAAACAAGCTGAGGCCATATTAGATATTAGTCTAAGAAGAATTACCCAGCTTGAGAGGAAAAAATTTGTAAATGAAAGTGAATCGTTAAAGGAGCAAATTTCAAAATTAGAAGAACTATTGTCAAGCAAGAAATCCATGTTACAGTTGATTGAACAAGAAGCAAACGAGTTAAAAAGCAAGTTTTCCAGTCCGAGGCGTTCAGTGCTGGAGGACAGTGATGGTGGCCACGTTGATGACATAGATGTTATTCCTAATGAAGAAATGCTTTTGGCCTTCAGTGAAAAGGGCTATGTTAAGCGGATGAAACCTAACACTTTCAATCTTCAAAATAGAGGAACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAACGATGCAATGTCAGACTTTATTGTTTGTCGCGCACATGATCATGTTCTATACTTCAGAGGAACAGTTTACTCTGGTCGTGCGTATAAAATTCCTGAATGCACCCGCACAGCTGCTGGGACGCCATTGGTTCAGATCTTAGCACTGTCGGATGGCGAAAGGATTACTTCTGTTATTCCTGTGAGTGAATTTGCTGAAGATCAATTTCTACTTATGCTTACAGTAAATGGCTACATAAAGAAGGTCTCTTTGAGTTCTTTTTCGTCAATCAGGTCAACAGGAATCATAGCAATCCAATTGGTTCCTGGTGATGAACTAAAATGGGTTCGTTGCTGTACAAATGATGACCTTGTAGCAATGGCTTCTCAGAATGGAATGGTCATCTTAAGTTCCTCTGGTATTATTCGAGCACAAGGGAGAAACACTAGGGGTGCTGTGGCCATGAGACTTAGAGAGGGGGATAAGATGGCTAGCGTGGACATTATACCAGCAGCTATGTGGAAAGACTTGGAGAGGGTGTCTGAAGCTCCCCAAGACGCTACTAGAAGTCTGAAGGGCCCGTGGTTGTTATTTGTGTCTGAGAGTGGATATGGAAAGCGTGTTCCCTTAAGTAGATTTCACTCCTCCCGGTTGAATAGGGTTGGTCTGATAGGTTACAAGTTTGCCTTAGAGGATCGCTTGGCAGCAGTTTTTGTGGTCGGGTTCTCTGTGGCAGAAGATGGAGAAAGCGATGAACAAGTGGTTCTTGTAAGCCAAAGTGGCACAGTCAACAGAATTAAGGTGCGGGATATTTCAATACAGTCCCGCTATGCAAGGGGAGTTATTTTGATGCGGCTAGATCATGCTGGAAAGATTCAGTCCGCTTCGTTAATCTCAGCAACGGACGAGGAGCCTGAGGAGGTAGAAGTTGAAGCTGCCGTACATGGCTAA

Protein Analysis

945

Amino Acids

104.24

Weight (kDa)

8.35

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 124 - 565 6.5e-160 DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C PF03989 599 - 645 2.1e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 704 - 747 3.9e-08 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 751 - 797 1.7e-06 DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C PF03989 881 - 926 3.5e-06 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 117
Acc36I ACCTGC 1 cut(s) 766
AccBSI CCGCTC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 1468
AccII CGCG 5 cut(s) 183, 306, 372, 454, 1938
AclWI GGATC 4 cut(s) 614, 1066, 1219, 2586
AcoI YGGCCR 3 cut(s) 367, 1759, 2343
AcsI RAATTY 7 cut(s) 503, 598, 721, 1598, 1632, 1995, 2093
AcuI CTGAAG 7 cut(s) 351, 1336, 1795, 1945, 2121, 2448, 2477
AcyI GRCGYC 1 cut(s) 2027
AfaI GTAC 2 cut(s) 2242, 2826
AfiI CCNNNNNNNGG 6 cut(s) 174, 226, 238, 322, 938, 1728
AflII CTTAAG 3 cut(s) 1202, 2290, 2512
AjnI CCWGG 3 cut(s) 173, 916, 2209
AleI CACNNNNGTG 1 cut(s) 527
AloI GAACNNNNNNTCC 4 cut(s) 589, 621, 2490, 2522
Alw21I GWGCWC 4 cut(s) 144, 315, 411, 2317
Alw26I GTCTC 7 cut(s) 19, 56, 125, 513, 1366, 2155, 2344
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 4 cut(s) 614, 1066, 1219, 2586
AlwNI CAGNNNCTG 1 cut(s) 2021
AoxI GGCC 9 cut(s) 36, 324, 367, 1438, 1548, 1759, 1806, 2343, 2461
ApaI GGGCCC 1 cut(s) 2465
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 8 cut(s) 626, 629, 1094, 1333, 2018, 2396, 2588, 2819
ApoI RAATTY 7 cut(s) 503, 598, 721, 1598, 1632, 1995, 2093
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 3 cut(s) 244, 374, 1940
AspS9I GGNCC 7 cut(s) 37, 177, 229, 920, 1438, 2461, 2462
AsuC2I CCSGG 2 cut(s) 234, 2533
AsuHPI GGTGA 2 cut(s) 248, 2225
AsuNHI GCTAGC 1 cut(s) 2375
AvaII GGWCC 3 cut(s) 177, 229, 920
AxyI CCTNAGG 1 cut(s) 2799
BaeGI GKGCMC 2 cut(s) 411, 2465
BalI TGGCCA 2 cut(s) 1761, 2345
BanII GRGCYC 3 cut(s) 144, 315, 2465
BarI GAAGNNNNNNTAC 1 cut(s) 2808
BbsI GAAGAC 2 cut(s) 54, 1417
Bbv12I GWGCWC 4 cut(s) 144, 315, 411, 2317
BbvCI CCTCAGC 1 cut(s) 1545
BbvI GCAGC 8 cut(s) 616, 638, 1106, 1320, 2005, 2408, 2600, 2806
BceAI ACGGC 2 cut(s) 512, 2807
BciT130I CCWGG 3 cut(s) 175, 918, 2211
BclI TGATCA 1 cut(s) 1945
BcnI CCSGG 2 cut(s) 234, 2533
BcoDI GTCTC 7 cut(s) 19, 56, 125, 513, 1366, 2155, 2344
BfaI CTAG 8 cut(s) 41, 477, 497, 905, 2331, 2376, 2448, 2741
BfmI CTRYAG 2 cut(s) 624, 1334
BfrI CTTAAG 3 cut(s) 1202, 2290, 2512
BfuAI ACCTGC 1 cut(s) 766
BglI GCCNNNNNGGC 2 cut(s) 341, 2664
BglII AGATCT 1 cut(s) 2040
Bme1390I CCNGG 5 cut(s) 175, 234, 918, 2211, 2533
Bme18I GGWCC 3 cut(s) 177, 229, 920
BmgT120I GGNCC 7 cut(s) 37, 177, 229, 920, 1438, 2461, 2462
BmiI GGNNCC 7 cut(s) 179, 206, 231, 1873, 2208, 2463, 2796
BmrFI CCNGG 5 cut(s) 175, 234, 918, 2211, 2533
BmsI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2724
BmtI GCTAGC 1 cut(s) 2379
BoxI GACNNNNGTC 1 cut(s) 182
BpiI GAAGAC 2 cut(s) 54, 1417
BpmI CTGGAG 2 cut(s) 957, 1763
Bpu10I CCTNAGC 3 cut(s) 555, 1308, 1545
BpuEI CTTGAG 1 cut(s) 1607
BpuMI CCSGG 2 cut(s) 234, 2533
BsaBI GATNNNNATC 2 cut(s) 618, 693
BsaHI GRCGYC 1 cut(s) 2027
BsaI GGTCTC 2 cut(s) 125, 2155
BsaJI CCNNGG 8 cut(s) 33, 232, 514, 540, 586, 958, 1727, 2465
BsaWI WCCGGW 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 2408, 2438
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 226, 238, 322, 938, 1728
Bse1I ACTGG 4 cut(s) 48, 940, 988, 1722
Bse21I CCTNAGG 1 cut(s) 2799
Bse3DI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2268
Bse8I GATNNNNATC 2 cut(s) 618, 693
BseBI CCWGG 3 cut(s) 175, 918, 2211
BseDI CCNNGG 8 cut(s) 33, 232, 514, 540, 586, 958, 1727, 2465
BseGI GGATG 6 cut(s) 69, 161, 508, 1168, 1842, 2062
BseJI GATNNNNATC 2 cut(s) 618, 693
BseLI CCNNNNNNNGG 6 cut(s) 174, 226, 238, 322, 938, 1728
BseMI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2268
BseMII CTCAG 9 cut(s) 24, 569, 1509, 1524, 1536, 2285, 2475, 2790, 2793
BseNI ACTGG 4 cut(s) 48, 940, 988, 1722
BseRI GAGGAG 3 cut(s) 2518, 2807, 2816
BseSI GKGCMC 2 cut(s) 411, 2465
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 8 cut(s) 616, 638, 1106, 1320, 2005, 2408, 2600, 2806
BseYI CCCAGC 2 cut(s) 1581, 2021
Bsh1236I CGCG 5 cut(s) 183, 306, 372, 454, 1938
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 9 cut(s) 38, 326, 369, 1440, 1550, 1761, 1808, 2345, 2463
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 4 cut(s) 144, 315, 411, 2317
BsiSI CCGG 4 cut(s) 117, 234, 415, 2533
BslFI GGGAC 2 cut(s) 2038, 2692
BslI CCNNNNNNNGG 6 cut(s) 174, 226, 238, 322, 938, 1728
BsmAI GTCTC 7 cut(s) 19, 56, 125, 513, 1366, 2155, 2344
BsmBI CGTCTC 1 cut(s) 1366
BsmFI GGGAC 2 cut(s) 2038, 2692
BsmI GAATGC 4 cut(s) 653, 1188, 1340, 2009
BsnI GGCC 9 cut(s) 38, 326, 369, 1440, 1550, 1761, 1808, 2345, 2463
Bso31I GGTCTC 2 cut(s) 125, 2155
Bsp120I GGGCCC 1 cut(s) 2461
Bsp1286I GDGCHC 5 cut(s) 144, 315, 411, 2317, 2465
Bsp1407I TGTACA 1 cut(s) 2240
Bsp19I CCATGG 2 cut(s) 33, 514
BspANI GGCC 9 cut(s) 38, 326, 369, 1440, 1550, 1761, 1808, 2345, 2463
BspCNI CTCAG 9 cut(s) 23, 568, 1510, 1525, 1537, 2284, 2476, 2791, 2792
BspFNI CGCG 5 cut(s) 183, 306, 372, 454, 1938
BspLI GGNNCC 7 cut(s) 179, 206, 231, 1873, 2208, 2463, 2796
BspMAI CTGCAG 2 cut(s) 628, 1338
BspMI ACCTGC 1 cut(s) 766
BspOI GCTAGC 1 cut(s) 2379
BspPI GGATC 4 cut(s) 614, 1066, 1219, 2586
BspTI CTTAAG 3 cut(s) 1202, 2290, 2512
BspTNI GGTCTC 2 cut(s) 125, 2155
BsrBI CCGCTC 1 cut(s) 97
BsrDI GCAATG 5 cut(s) 435, 681, 1495, 1920, 2268
BsrGI TGTACA 1 cut(s) 2240
BsrI ACTGG 4 cut(s) 48, 940, 988, 1722
BssECI CCNNGG 8 cut(s) 33, 232, 514, 540, 586, 958, 1727, 2465
BssNAI GTATAC 1 cut(s) 1469
BssNI GRCGYC 1 cut(s) 2027
BssT1I CCWWGG 5 cut(s) 33, 514, 540, 586, 958
Bst1107I GTATAC 1 cut(s) 1469
Bst2UI CCWGG 3 cut(s) 175, 918, 2211
Bst6I CTCTTC 1 cut(s) 1263
BstACI GRCGYC 1 cut(s) 2027
BstAFI CTTAAG 3 cut(s) 1202, 2290, 2512
BstAUI TGTACA 1 cut(s) 2240
BstC8I GCNNGC 2 cut(s) 1338, 2377
BstDSI CCRYGG 3 cut(s) 33, 514, 2465
BstF5I GGATG 6 cut(s) 69, 161, 508, 1168, 1842, 2062
BstFNI CGCG 5 cut(s) 183, 306, 372, 454, 1938
BstHHI GCGC 3 cut(s) 244, 374, 1940
BstMAI GTCTC 7 cut(s) 19, 56, 125, 513, 1366, 2155, 2344
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 7 cut(s) 288, 332, 341, 623, 1309, 1738, 2664
BstNI CCWGG 3 cut(s) 175, 918, 2211
BstNSI RCATGY 1 cut(s) 916
BstPAI GACNNNNGTC 1 cut(s) 182
BstSCI CCNGG 5 cut(s) 173, 232, 916, 2209, 2531
BstSFI CTRYAG 2 cut(s) 624, 1334
BstSLI GKGCMC 2 cut(s) 411, 2465
BstUI CGCG 5 cut(s) 183, 306, 372, 454, 1938
BstV1I GCAGC 8 cut(s) 616, 638, 1106, 1320, 2005, 2408, 2600, 2806
BstV2I GAAGAC 2 cut(s) 54, 1417
BstX2I RGATCY 3 cut(s) 1071, 1211, 2040
BstXI CCANNNNNNTGG 2 cut(s) 515, 593
BstYI RGATCY 3 cut(s) 1071, 1211, 2040
BstZ17I GTATAC 1 cut(s) 1469
BstZI CGGCCG 1 cut(s) 367
Bsu36I CCTNAGG 1 cut(s) 2799
BsuRI GGCC 9 cut(s) 38, 326, 369, 1440, 1550, 1761, 1808, 2345, 2463
BtgI CCRYGG 3 cut(s) 33, 514, 2465
BtgZI GCGATG 1 cut(s) 2649
BtsCI GGATG 6 cut(s) 69, 161, 508, 1168, 1842, 2062
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 7 cut(s) 327, 522, 662, 1743, 1756, 1819, 2048
BveI ACCTGC 1 cut(s) 766
Cac8I GCNNGC 2 cut(s) 1338, 2377
CaiI CAGNNNCTG 1 cut(s) 2021
CfoI GCGC 3 cut(s) 244, 374, 1940
Cfr13I GGNCC 7 cut(s) 37, 177, 229, 920, 1438, 2461, 2462
CseI GACGC 4 cut(s) 172, 980, 2035, 2450
Csp6I GTAC 2 cut(s) 2241, 2825
CviQI GTAC 2 cut(s) 2241, 2825
DrdI GACNNNNNNGTC 1 cut(s) 117
DseDI GACNNNNNNGTC 1 cut(s) 117
EaeI YGGCCR 3 cut(s) 367, 1759, 2343
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
EciI GGCGGA 2 cut(s) 113, 176
Ecl136II GAGCTC 2 cut(s) 142, 313
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 5 cut(s) 33, 514, 540, 586, 958
Eco24I GRGCYC 3 cut(s) 144, 315, 2465
Eco31I GGTCTC 2 cut(s) 125, 2155
Eco47I GGWCC 3 cut(s) 177, 229, 920
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 2 cut(s) 142, 313
Eco57I CTGAAG 7 cut(s) 351, 1336, 1795, 1945, 2121, 2448, 2477
Eco81I CCTNAGG 1 cut(s) 2799
EcoICRI GAGCTC 2 cut(s) 142, 313
EcoO109I RGGNCCY 3 cut(s) 177, 229, 2461
EcoRII CCWGG 3 cut(s) 173, 916, 2209
EcoT14I CCWWGG 5 cut(s) 33, 514, 540, 586, 958
EcoT38I GRGCYC 3 cut(s) 144, 315, 2465
ErhI CCWWGG 5 cut(s) 33, 514, 540, 586, 958
Esp3I CGTCTC 1 cut(s) 1366
FaqI GGGAC 2 cut(s) 2038, 2692
FauI CCCGC 6 cut(s) 102, 176, 188, 2018, 2682, 2717
FbaI TGATCA 1 cut(s) 1945
FblI GTMKAC 1 cut(s) 1468
FokI GGATG 6 cut(s) 56, 148, 495, 1175, 1849, 2069
FriOI GRGCYC 3 cut(s) 144, 315, 2465
FspBI CTAG 8 cut(s) 41, 477, 497, 905, 2331, 2376, 2448, 2741
GlaI GCGC 3 cut(s) 243, 373, 1939
GsaI CCCAGC 2 cut(s) 1585, 2025
GsuI CTGGAG 2 cut(s) 957, 1763
HaeIII GGCC 9 cut(s) 38, 326, 369, 1440, 1550, 1761, 1808, 2345, 2463
HapII CCGG 4 cut(s) 117, 234, 415, 2533
HgaI GACGC 4 cut(s) 172, 980, 2035, 2450
HhaI GCGC 3 cut(s) 244, 374, 1940
Hin1I GRCGYC 1 cut(s) 2027
Hin6I GCGC 3 cut(s) 242, 372, 1938
HinP1I GCGC 3 cut(s) 242, 372, 1938
HincII GTYRAC 2 cut(s) 2181, 2674
HindII GTYRAC 2 cut(s) 2181, 2674
HindIII AAGCTT 1 cut(s) 1479
HinfI GANTC 9 cut(s) 111, 536, 573, 963, 1034, 1454, 1616, 2187, 2758
HpaII CCGG 4 cut(s) 117, 234, 415, 2533
HphI GGTGA 2 cut(s) 248, 2225
Hpy166II GTNNAC 7 cut(s) 409, 1469, 1906, 1975, 2181, 2383, 2674
Hpy188III TCNNGA 7 cut(s) 389, 466, 557, 881, 1179, 1369, 2000
Hpy8I GTNNAC 7 cut(s) 409, 1469, 1906, 1975, 2181, 2383, 2674
HpyAV CCTTC 4 cut(s) 1136, 1819, 2140, 2452
HpyCH4IV ACGT 3 cut(s) 890, 994, 1764
HpyF10VI GCNNNNNNNGC 7 cut(s) 288, 332, 341, 623, 1309, 1738, 2664
HpySE526I ACGT 3 cut(s) 890, 994, 1764
Hsp92I GRCGYC 1 cut(s) 2027
HspAI GCGC 3 cut(s) 242, 372, 1938
Ksp22I TGATCA 1 cut(s) 1945
LmnI GCTCC 7 cut(s) 139, 147, 210, 318, 1627, 2437, 2794
Lsp1109I GCAGC 8 cut(s) 616, 638, 1106, 1320, 2005, 2408, 2600, 2806
LweI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2724
MaeI CTAG 8 cut(s) 41, 477, 497, 905, 2331, 2376, 2448, 2741
MaeII ACGT 3 cut(s) 890, 994, 1764
MaeIII GTNAC 5 cut(s) 389, 638, 1277, 1674, 2558
MbiI CCGCTC 1 cut(s) 97
MfeI CAATTG 2 cut(s) 1224, 2201
MflI RGATCY 3 cut(s) 1071, 1211, 2040
MhlI GDGCHC 5 cut(s) 144, 315, 411, 2317, 2465
MlsI TGGCCA 2 cut(s) 1761, 2345
MluNI TGGCCA 2 cut(s) 1761, 2345
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 4 cut(s) 466, 794, 956, 2034
Mox20I TGGCCA 2 cut(s) 1761, 2345
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 2 cut(s) 1761, 2345
MslI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
Msp20I TGGCCA 2 cut(s) 1761, 2345
MspA1I CMGCKG 3 cut(s) 629, 1505, 2018
MspCI CTTAAG 3 cut(s) 1202, 2290, 2512
MspI CCGG 4 cut(s) 117, 234, 415, 2533
MspR9I CCNGG 5 cut(s) 175, 234, 918, 2211, 2533
MunI CAATTG 2 cut(s) 1224, 2201
Mva1269I GAATGC 4 cut(s) 653, 1188, 1340, 2009
MvaI CCWGG 3 cut(s) 175, 918, 2211
MvnI CGCG 5 cut(s) 183, 306, 372, 454, 1938
MwoI GCNNNNNNNGC 7 cut(s) 288, 332, 341, 623, 1309, 1738, 2664
NciI CCSGG 2 cut(s) 234, 2533
NcoI CCATGG 2 cut(s) 33, 514
NheI GCTAGC 1 cut(s) 2375
NlaIV GGNNCC 7 cut(s) 179, 206, 231, 1873, 2208, 2463, 2796
NmeAIII GCCGAG 1 cut(s) 345
NmuCI GTSAC 1 cut(s) 389
NspI RCATGY 1 cut(s) 916
OliI CACNNNNGTG 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 458
PctI GAATGC 4 cut(s) 653, 1188, 1340, 2009
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 8 cut(s) 536, 573, 963, 1034, 1454, 1616, 2187, 2758
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
PpuMI RGGWCCY 2 cut(s) 177, 229
PshAI GACNNNNGTC 1 cut(s) 182
Psp124BI GAGCTC 2 cut(s) 144, 315
Psp5II RGGWCCY 2 cut(s) 177, 229
Psp6I CCWGG 3 cut(s) 173, 916, 2209
PspFI CCCAGC 2 cut(s) 1581, 2021
PspGI CCWGG 3 cut(s) 173, 916, 2209
PspN4I GGNNCC 7 cut(s) 179, 206, 231, 1873, 2208, 2463, 2796
PspOMI GGGCCC 1 cut(s) 2461
PspPI GGNCC 7 cut(s) 37, 177, 229, 920, 1438, 2461, 2462
PspPPI RGGWCCY 2 cut(s) 177, 229
PstI CTGCAG 2 cut(s) 628, 1338
PstNI CAGNNNCTG 1 cut(s) 2021
PsuI RGATCY 3 cut(s) 1071, 1211, 2040
PvuII CAGCTG 3 cut(s) 629, 1505, 2018
RsaI GTAC 2 cut(s) 2242, 2826
RsaNI GTAC 2 cut(s) 2241, 2825
RseI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
SacI GAGCTC 2 cut(s) 144, 315
Sau96I GGNCC 7 cut(s) 37, 177, 229, 920, 1438, 2461, 2462
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 5 cut(s) 175, 234, 918, 2211, 2533
SduI GDGCHC 5 cut(s) 144, 315, 411, 2317, 2465
SfaNI GCATC 6 cut(s) 167, 604, 1346, 1508, 1900, 2724
SfcI CTRYAG 2 cut(s) 624, 1334
SinI GGWCC 3 cut(s) 177, 229, 920
SmiMI CAYNNNNRTG 5 cut(s) 513, 527, 1433, 1767, 1947
SmlI CTYRAG 4 cut(s) 1202, 1586, 2290, 2512
SmoI CTYRAG 4 cut(s) 1202, 1586, 2290, 2512
SspMI CTAG 8 cut(s) 41, 477, 497, 905, 2331, 2376, 2448, 2741
SstI GAGCTC 2 cut(s) 144, 315
StyD4I CCNGG 5 cut(s) 173, 232, 916, 2209, 2531
StyI CCWWGG 5 cut(s) 33, 514, 540, 586, 958
TaiI ACGT 3 cut(s) 893, 997, 1767
TaqI TCGA 4 cut(s) 763, 979, 1368, 2311
TatI WGTACW 1 cut(s) 2240
TauI GCSGC 3 cut(s) 92, 372, 2740
TfiI GAWTC 8 cut(s) 536, 573, 963, 1034, 1454, 1616, 2187, 2758
TscAI CASTG 7 cut(s) 334, 529, 669, 1743, 1756, 1819, 2055
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 8 cut(s) 626, 629, 1094, 1333, 2018, 2396, 2588, 2819
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 8 cut(s) 17, 1179, 1283, 1623, 1806, 1853, 2232, 2652
TspGWI ACGGA 2 cut(s) 659, 2801
TspRI CASTG 7 cut(s) 334, 529, 669, 1743, 1756, 1819, 2055
Vha464I CTTAAG 3 cut(s) 1202, 2290, 2512
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 3 cut(s) 177, 229, 920
XapI RAATTY 7 cut(s) 503, 598, 721, 1598, 1632, 1995, 2093
XceI RCATGY 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 2 cut(s) 2207, 2400
XmiI GTMKAC 1 cut(s) 1468
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 8 cut(s) 41, 477, 497, 905, 2331, 2376, 2448, 2741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.