Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010284

Physical Location & Seq

Genomic Coordinates Forward (+)

5003932 .. 5019821

Gene Structure 15890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1054300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGAAACCCTCAGTCTCCCTCCACCCCCCTACCATGGCCCTAGCTTCCGGTCTCCGCTTGTCTTCCTCCATCCTCCGCTACCGCCTCGCCGCCCCGCTCAACCCGACCCGACTTTCCGGTCTCCGCCACAACCTCTCGGAGCTCCGCTTCCTTTCAGCATCATCCTCCCGCCCCAGGACCCGCGTCCGCCCAATTAAAGCGACGCTCCTTGACGAACCCCAAAAGGAGGACCCTGGCGAGGGGCAAGATGGGAACGGGAGTGTGCTGGTCAAGGACACAGGCGAGAATAGCGAAAGGATTGTCCGTGTTGAGCTCCACAAGGAGGCCACTGAAGCCTACATGGCCTACGCTATGTCTGTCCTGCTCGGCCGCGCCCTGCCTGATGTTCGTGATGGTTTGAAGCCCGTGCACCGCCGGATTTTGTATGCAATGCACGAATTGGGGATTGTGTCGCGGAAACCCTTCAAGAAATGTGCTAGAGTTGTTGGAGAGGTTCTAGGAAAATTCCATCCCCATGGAGACACTGCTGTGTACGATTCGTTGGTGCGAATGGCTCAGGATTTCTCCTTACGATTCCCACTAATCCAAGGGCATGGGAATTTTGGTTCTATTGATGCTGATCCTGCAGCTGCCATGCGTTACACGGAATGCAGATTGGAAGCACTGACAGAAGCAATGCTATTGGCAGACTTAGATCAAAATACGGTTGATTTTACTCCAAATTTTGATAATTCACAAAAAGAGCCATCAGTTTTACCTGCTCGACTTCCAACTTTATTACTGAATGGTGCTTCTGGGATTGCGGTCGGCATGGCAACAAACATTCCTCCGCATAATCTTGGGGAATTGGTAGATGTTCTTAGCGTGTTAATTCACAATCCAGAAGCCACGTTGCAAGAATTACTAGAATACATGCCAGGACCCGATTTTCCAACTGGAGGACTGATTATGGGCAACCTTGGAATCTTAGATGCATATCGAACCGGAAAAGGACGTATTGTTGTAAGAGGGAAAACTGATGTTGAATTGCTTGATTCTAAAACAAAGAGAAATGCAGTTATCATAAAAGAGATCCCTTACCAAACCAACAAAGCAGCTCTTGTGGAGAAGATAGCGGAACTTGTGGAAAATAAGATTTTAGAAGGCATAAGTGATATTAGGGACGAAAGTGATCGTTCTGGAATGCGTGTAGTCATTGAGCTCAAGCGAGGATCCGACCCATCAATTGTTTTAAATAATCTCTATCGCCTCACATCTCTTCAATGCAGTTTTAGTTGTAACATGGTGGGTATTCATAATGGGCAACCTAAGCAGATGGGTCTAAAGGAATTGCTGCAGGCATTCTTAGACTTCAGATGCTTTGTTGTTGAGAGACGTGCTAAGTTCAAACTTTCACAAGCACAGGACAGAAGACATATTGTTGAGGGCATTGTTGTGGGCCTTGATAATTTGGATGCAGTAATTCGTATACTAAGGGAAGCTTCGAGCAATGCAATTGCATCAGCTGGTTTAAGGACTGAGTTCAATCTTTCTGAGAAACAAGCTGAAGCCATATTAGATATTAGCCTAAGAAGAATTACCCAGCTTGAGAGGAAAAAGTTTGTAAATGAAAGTGAATCATTAAAGGAGCAAATTTCAAAATTAGAACAACTATTGTCAAGCAAGAAATCCATGTTACAGTTGATTGAACAAGAAGCAAACGAGTTAAAAAGCAAGTTTTCCAGTCCGAGGCGTTCCGTGCTAGAGGACAGTGATGGTGGCCACGTTGATGACATAGATGTTATTCCTAATGAAGAAATGCTTTTGGCCTTCAGTGAAAAGGGCTATGTTAAGCGGATGAAACCTAACACTTTCAATCTTCAAAATAGAGGAACCATTGGTAAATCTGTTGGGAAATTGAGGGTAAATGATGCAATGTCAGACTTTATTGTTTGTCGCGCGCACGATCATGTTCTATACTTTAGTGATAAAGGAACAGTTTACTCTGGTCGTGCATATAAAATTCCTGAATGCACTCGCACAGCTGCTGGGACGCCATTGGTTCAGATCTTAGCACTGTCAGATGGTGAAAGGATTACTTCTGTTATTCCTGTGAGTGAATTTGCTGGAGATCAGTTTCTACTTATGCTTACAGTGAATGGATACATCAAGAAGGTCTCTTTGAGTTCTTTTTCGTCAATCAGGTCAACAGGAATCATAGCAATCCAATTGGTTCCTGGTGATGAACTAAAATGGGTTCGTTGCTGCACAAACGATGACCTTGTAGCGATGGCCTCTCAGAATGGAATGGTCATCTTAAGTTCCTCTGGTATAATTCGAGCACAAGGGAGAAACACTAGGGGTGCAGTGGCCATGAGACTTAGAGAGGGGGATAAGATGGCTAGCGTGGACATTATACCAGCAGCTATGTGGAAAGACTTGGAGAGGGTGTCTGAAGCTCCCCAAGACACTACTAGAAGTCTGAAGGGCCCGTGGTTATTATTTGTTTCTGAGAGTGGATTTGGAAAGCGTGTTCCCTTGAGCAGGTTTCACTCCTCCCGCTTGAATAGGGTTGGTCTGATAGGGTACAAGTTTGCCTTAGAGGATTGCTTGGCAGCAGTTTTTGTGGTCGGGTTCTCTGTGGCAGAAGATGGAGAAAGCGATGAACAAGTGGTTCTAGTAAGCCAAAGCGGCACAGTCAACAGAATTAAGGTGCAGGATATTTCAATACAGTCCCGCTATGCGAGGGGAGTTATTTTAATGCGGCTTGATCATGCTGGAAAGATTCAGTCTGCTTCGTTAATGTCAGCAACGGACGGGGAGCCTGAGGACGTAGAAGTTGAAGCTGCCGTACATGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0009295 GO:0009507 GO:0009536 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_gyraseA_C DNA_topoisoIV

Protein Analysis

947

Amino Acids

104.15

Weight (kDa)

8.15

Isoelectric Point (pI)

40.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_topoisoIV	PF00521	124 - 565	1.6e-158	DNA gyrase/topoisomerase IV, subunit A
DNA_gyraseA_C	PF03989	599 - 645	2.1e-08	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	706 - 749	3.9e-08	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	753 - 799	1.7e-06	DNA gyrase C-terminal domain, beta-propeller
DNA_gyraseA_C	PF03989	883 - 924	5.9e-06	DNA gyrase C-terminal domain, beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010284)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10690
fragaria_vesca	FvH4_7g05000 FvH4_7g05000
malus_domestica	MD02G1265100.v1.1 MD07G1054300.v1.1
prunus_persica	Prupe.2G060900_v2.0.a1 Prupe.2G060900_v2.0.a1
pyrus_communis	pycom02g22650 pycom07g04200
rosa_chinensis	RchiOBHm_Chr1g0331271
rosa_laevigata	RLG00000029745
rosa_multiflora	Rmu_co8191516.1_g000001 Rmu_sc0000597.1_g000001
rosa_roxburghii	Rroxscaffold_4G00319250
rosa_rugosa	Rorug01G0092400 Rorug01G0092500
rosa_samantha	Rh1AG117100 Rh1BG087900 Rh1CG112100 Rh1DG120900
rosa_wichuraiana	Rw1G009370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	117
Acc36I	ACCTGC	2 cut(s)	766, 2514
AccBSI	CCGCTC	1 cut(s)	97
AccI	GTMKAC	1 cut(s)	1468
AccII	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
AclWI	GGATC	4 cut(s)	614, 1066, 1206, 1219
AcoI	YGGCCR	3 cut(s)	367, 1759, 2349
AcsI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
AcuI	CTGAAG	6 cut(s)	351, 1336, 1566, 1795, 2454, 2483
AcyI	GRCGYC	1 cut(s)	2033
AfaI	GTAC	3 cut(s)	533, 2567, 2832
AfiI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 322, 938, 1728, 2523
AflII	CTTAAG	1 cut(s)	2296
AjiI	CACGTC	1 cut(s)	1376
AjnI	CCWGG	4 cut(s)	173, 232, 916, 2215
AleI	CACNNNNGTG	1 cut(s)	527
AloI	GAACNNNNNNTCC	4 cut(s)	589, 621, 2496, 2528
Alw21I	GWGCWC	5 cut(s)	144, 315, 411, 1203, 2323
Alw26I	GTCTC	7 cut(s)	19, 56, 125, 513, 1366, 2161, 2350
Alw44I	GTGCAC	1 cut(s)	407
AlwI	GGATC	4 cut(s)	614, 1066, 1206, 1219
AlwNI	CAGNNNCTG	1 cut(s)	2027
ApaI	GGGCCC	1 cut(s)	2471
ApaLI	GTGCAC	1 cut(s)	407
ApeKI	GCWGC	9 cut(s)	626, 629, 1094, 1333, 2024, 2244, 2402, 2594, 2825
ApoI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
ArsI	GACNNNNNNTTYG	2 cut(s)	285, 317
Asp700I	GAANNNNTTC	1 cut(s)	461
AspLEI	GCGC	3 cut(s)	374, 1940, 1942
AspS9I	GGNCC	7 cut(s)	37, 177, 229, 920, 1438, 2467, 2468
AsuHPI	GGTGA	2 cut(s)	2078, 2231
AsuNHI	GCTAGC	1 cut(s)	2381
AvaII	GGWCC	3 cut(s)	177, 229, 920
AxyI	CCTNAGG	1 cut(s)	2805
BaeGI	GKGCMC	2 cut(s)	411, 2471
BalI	TGGCCA	2 cut(s)	1761, 2351
BamHI	GGATCC	1 cut(s)	1211
BanII	GRGCYC	4 cut(s)	144, 315, 1203, 2471
BarI	GAAGNNNNNNTAC	1 cut(s)	2814
BbsI	GAAGAC	2 cut(s)	54, 1417
Bbv12I	GWGCWC	5 cut(s)	144, 315, 411, 1203, 2323
BbvI	GCAGC	9 cut(s)	616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BceAI	ACGGC	1 cut(s)	2813
BciT130I	CCWGG	4 cut(s)	175, 234, 918, 2217
BciVI	GTATCC	1 cut(s)	2135
BclI	TGATCA	1 cut(s)	2749
BcoDI	GTCTC	7 cut(s)	19, 56, 125, 513, 1366, 2161, 2350
BfaI	CTAG	9 cut(s)	41, 477, 497, 905, 1742, 2337, 2382, 2454, 2657
BfmI	CTRYAG	2 cut(s)	624, 1334
BfrI	CTTAAG	1 cut(s)	2296
BfuAI	ACCTGC	2 cut(s)	766, 2514
BfuI	GTATCC	1 cut(s)	2135
BglI	GCCNNNNNGGC	2 cut(s)	341, 2670
BglII	AGATCT	1 cut(s)	2046
Bme1390I	CCNGG	4 cut(s)	175, 234, 918, 2217
Bme18I	GGWCC	3 cut(s)	177, 229, 920
BmgBI	CACGTC	1 cut(s)	1376
BmgT120I	GGNCC	7 cut(s)	37, 177, 229, 920, 1438, 2467, 2468
BmiI	GGNNCC	8 cut(s)	179, 231, 922, 1213, 1873, 2214, 2469, 2802
BmrFI	CCNGG	4 cut(s)	175, 234, 918, 2217
BmsI	GCATC	7 cut(s)	167, 604, 961, 1346, 1444, 1508, 1900
BmtI	GCTAGC	1 cut(s)	2385
BoxI	GACNNNNGTC	1 cut(s)	182
BpiI	GAAGAC	2 cut(s)	54, 1417
BpmI	CTGGAG	2 cut(s)	957, 2127
Bpu10I	CCTNAGC	2 cut(s)	555, 1308
BpuEI	CTTGAG	3 cut(s)	1187, 1607, 2539
BsaBI	GATNNNNATC	2 cut(s)	618, 975
BsaHI	GRCGYC	1 cut(s)	2033
BsaI	GGTCTC	3 cut(s)	56, 125, 2161
BsaJI	CCNNGG	8 cut(s)	33, 173, 232, 514, 586, 958, 1727, 2471
BsaWI	WCCGGW	3 cut(s)	47, 116, 983
BsaXI	ACNNNNNCTCC	2 cut(s)	2414, 2444
Bsc4I	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 322, 938, 1728, 2523
Bse1I	ACTGG	2 cut(s)	940, 1722
Bse21I	CCTNAGG	1 cut(s)	2805
Bse3DI	GCAATG	4 cut(s)	435, 681, 1495, 1920
Bse8I	GATNNNNATC	2 cut(s)	618, 975
BseBI	CCWGG	4 cut(s)	175, 234, 918, 2217
BseDI	CCNNGG	8 cut(s)	33, 173, 232, 514, 586, 958, 1727, 2471
BseGI	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BseJI	GATNNNNATC	2 cut(s)	618, 975
BseLI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 322, 938, 1728, 2523
BseMI	GCAATG	4 cut(s)	435, 681, 1495, 1920
BseMII	CTCAG	7 cut(s)	24, 569, 1509, 1524, 2291, 2481, 2796
BseNI	ACTGG	2 cut(s)	940, 1722
BsePI	GCGCGC	1 cut(s)	1938
BseRI	GAGGAG	1 cut(s)	2524
BseSI	GKGCMC	2 cut(s)	411, 2471
BseX3I	CGGCCG	1 cut(s)	367
BseXI	GCAGC	9 cut(s)	616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BseYI	CCCAGC	2 cut(s)	1581, 2027
BsgI	GTGCAG	3 cut(s)	2230, 2364, 2714
Bsh1236I	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
Bsh1285I	CGRYCG	2 cut(s)	370, 807
BsiEI	CGRYCG	2 cut(s)	370, 807
BsiHKAI	GWGCWC	5 cut(s)	144, 315, 411, 1203, 2323
BsiSI	CCGG	4 cut(s)	48, 117, 415, 984
BslFI	GGGAC	3 cut(s)	1175, 2044, 2698
BslI	CCNNNNNNNGG	9 cut(s)	34, 174, 226, 238, 239, 322, 938, 1728, 2523
BsmAI	GTCTC	7 cut(s)	19, 56, 125, 513, 1366, 2161, 2350
BsmBI	CGTCTC	1 cut(s)	1366
BsmFI	GGGAC	3 cut(s)	1175, 2044, 2698
BsmI	GAATGC	4 cut(s)	653, 1188, 1340, 2015
Bso31I	GGTCTC	3 cut(s)	56, 125, 2161
Bsp120I	GGGCCC	1 cut(s)	2467
Bsp1286I	GDGCHC	6 cut(s)	144, 315, 411, 1203, 2323, 2471
Bsp143I	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
Bsp19I	CCATGG	2 cut(s)	33, 514
BspCNI	CTCAG	7 cut(s)	23, 568, 1510, 1525, 2290, 2482, 2797
BspFNI	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
BspLI	GGNNCC	8 cut(s)	179, 231, 922, 1213, 1873, 2214, 2469, 2802
BspMAI	CTGCAG	2 cut(s)	628, 1338
BspMI	ACCTGC	2 cut(s)	766, 2514
BspOI	GCTAGC	1 cut(s)	2385
BspPI	GGATC	4 cut(s)	614, 1066, 1206, 1219
BspTI	CTTAAG	1 cut(s)	2296
BspTNI	GGTCTC	3 cut(s)	56, 125, 2161
BsrBI	CCGCTC	1 cut(s)	97
BsrDI	GCAATG	4 cut(s)	435, 681, 1495, 1920
BsrI	ACTGG	2 cut(s)	940, 1722
BssECI	CCNNGG	8 cut(s)	33, 173, 232, 514, 586, 958, 1727, 2471
BssHII	GCGCGC	1 cut(s)	1938
BssMI	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
BssNAI	GTATAC	1 cut(s)	1469
BssNI	GRCGYC	1 cut(s)	2033
BssT1I	CCWWGG	4 cut(s)	33, 514, 586, 958
Bst1107I	GTATAC	1 cut(s)	1469
Bst2UI	CCWGG	4 cut(s)	175, 234, 918, 2217
Bst4CI	ACNGT	8 cut(s)	706, 1680, 1751, 1978, 2058, 2134, 2677, 2712
Bst6I	CTCTTC	1 cut(s)	1263
BstACI	GRCGYC	1 cut(s)	2033
BstAFI	CTTAAG	1 cut(s)	2296
BstC8I	GCNNGC	3 cut(s)	1338, 1940, 2383
BstDSI	CCRYGG	3 cut(s)	33, 514, 2471
BstENI	CCTNNNNNAGG	1 cut(s)	2521
BstF5I	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BstFNI	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
BstHHI	GCGC	3 cut(s)	374, 1940, 1942
BstKTI	GATC	9 cut(s)	622, 697, 1074, 1174, 1214, 1948, 2049, 2113, 2752
BstMAI	GTCTC	7 cut(s)	19, 56, 125, 513, 1366, 2161, 2350
BstMBI	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
BstMCI	CGRYCG	2 cut(s)	370, 807
BstMWI	GCNNNNNNNGC	7 cut(s)	288, 332, 341, 623, 1309, 1738, 2670
BstNI	CCWGG	4 cut(s)	175, 234, 918, 2217
BstNSI	RCATGY	1 cut(s)	916
BstPAI	GACNNNNGTC	1 cut(s)	182
BstSCI	CCNGG	4 cut(s)	173, 232, 916, 2215
BstSFI	CTRYAG	2 cut(s)	624, 1334
BstSLI	GKGCMC	2 cut(s)	411, 2471
BstUI	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
BstV1I	GCAGC	9 cut(s)	616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
BstV2I	GAAGAC	2 cut(s)	54, 1417
BstX2I	RGATCY	3 cut(s)	1071, 1211, 2046
BstXI	CCANNNNNNTGG	2 cut(s)	515, 593
BstYI	RGATCY	3 cut(s)	1071, 1211, 2046
BstZ17I	GTATAC	1 cut(s)	1469
BstZI	CGGCCG	1 cut(s)	367
Bsu36I	CCTNAGG	1 cut(s)	2805
BsuI	GTATCC	1 cut(s)	2135
BtgI	CCRYGG	3 cut(s)	33, 514, 2471
BtgZI	GCGATG	2 cut(s)	2282, 2655
BtrI	CACGTC	1 cut(s)	1376
BtsCI	GGATG	5 cut(s)	69, 161, 508, 1459, 1842
BtsI	GCAGTG	2 cut(s)	522, 2352
BtsIMutI	CAGTG	8 cut(s)	327, 522, 662, 1756, 1819, 2054, 2139, 2352
BveI	ACCTGC	2 cut(s)	766, 2514
Cac8I	GCNNGC	3 cut(s)	1338, 1940, 2383
CaiI	CAGNNNCTG	1 cut(s)	2027
CfoI	GCGC	3 cut(s)	374, 1940, 1942
Cfr13I	GGNCC	7 cut(s)	37, 177, 229, 920, 1438, 2467, 2468
CseI	GACGC	3 cut(s)	172, 211, 2041
Csp6I	GTAC	3 cut(s)	532, 2566, 2831
CviQI	GTAC	3 cut(s)	532, 2566, 2831
DpnI	GATC	9 cut(s)	621, 696, 1073, 1173, 1213, 1947, 2048, 2112, 2751
DpnII	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
DraI	TTTAAA	1 cut(s)	1233
DrdI	GACNNNNNNGTC	1 cut(s)	117
DseDI	GACNNNNNNGTC	1 cut(s)	117
EaeI	YGGCCR	3 cut(s)	367, 1759, 2349
EagI	CGGCCG	1 cut(s)	367
Eam1104I	CTCTTC	1 cut(s)	1263
EarI	CTCTTC	1 cut(s)	1263
EciI	GGCGGA	2 cut(s)	113, 176
Ecl136II	GAGCTC	3 cut(s)	142, 313, 1201
EclXI	CGGCCG	1 cut(s)	367
Eco130I	CCWWGG	4 cut(s)	33, 514, 586, 958
Eco24I	GRGCYC	4 cut(s)	144, 315, 1203, 2471
Eco31I	GGTCTC	3 cut(s)	56, 125, 2161
Eco47I	GGWCC	3 cut(s)	177, 229, 920
Eco52I	CGGCCG	1 cut(s)	367
Eco53kI	GAGCTC	3 cut(s)	142, 313, 1201
Eco57I	CTGAAG	6 cut(s)	351, 1336, 1566, 1795, 2454, 2483
Eco81I	CCTNAGG	1 cut(s)	2805
EcoICRI	GAGCTC	3 cut(s)	142, 313, 1201
EcoNI	CCTNNNNNAGG	1 cut(s)	2521
EcoO109I	RGGNCCY	4 cut(s)	177, 229, 920, 2467
EcoRII	CCWGG	4 cut(s)	173, 232, 916, 2215
EcoT14I	CCWWGG	4 cut(s)	33, 514, 586, 958
EcoT22I	ATGCAT	1 cut(s)	976
EcoT38I	GRGCYC	4 cut(s)	144, 315, 1203, 2471
ErhI	CCWWGG	4 cut(s)	33, 514, 586, 958
Esp3I	CGTCTC	1 cut(s)	1366
FaqI	GGGAC	3 cut(s)	1175, 2044, 2698
FauI	CCCGC	5 cut(s)	102, 176, 188, 2546, 2723
FbaI	TGATCA	1 cut(s)	2749
FblI	GTMKAC	1 cut(s)	1468
FokI	GGATG	5 cut(s)	56, 148, 495, 1466, 1849
FriOI	GRGCYC	4 cut(s)	144, 315, 1203, 2471
FspBI	CTAG	9 cut(s)	41, 477, 497, 905, 1742, 2337, 2382, 2454, 2657
GlaI	GCGC	3 cut(s)	373, 1939, 1941
GsaI	CCCAGC	2 cut(s)	1585, 2031
GsuI	CTGGAG	2 cut(s)	957, 2127
HapII	CCGG	4 cut(s)	48, 117, 415, 984
HgaI	GACGC	3 cut(s)	172, 211, 2041
HhaI	GCGC	3 cut(s)	374, 1940, 1942
Hin1I	GRCGYC	1 cut(s)	2033
Hin6I	GCGC	3 cut(s)	372, 1938, 1940
HinP1I	GCGC	3 cut(s)	372, 1938, 1940
HincII	GTYRAC	2 cut(s)	2187, 2680
HindII	GTYRAC	2 cut(s)	2187, 2680
HindIII	AAGCTT	1 cut(s)	1479
HinfI	GANTC	7 cut(s)	536, 573, 963, 1034, 1616, 2193, 2764
HpaII	CCGG	4 cut(s)	48, 117, 415, 984
HphI	GGTGA	2 cut(s)	2078, 2231
Hpy166II	GTNNAC	7 cut(s)	409, 532, 1469, 1981, 2187, 2389, 2680
Hpy188III	TCNNGA	7 cut(s)	389, 466, 557, 881, 1179, 2006, 2149
Hpy8I	GTNNAC	7 cut(s)	409, 532, 1469, 1981, 2187, 2389, 2680
Hpy99I	CGWCG	1 cut(s)	205
HpyAV	CCTTC	5 cut(s)	472, 1136, 1819, 2146, 2458
HpyCH4III	ACNGT	8 cut(s)	706, 1680, 1751, 1978, 2058, 2134, 2677, 2712
HpyCH4IV	ACGT	5 cut(s)	890, 994, 1375, 1764, 2811
HpyF10VI	GCNNNNNNNGC	7 cut(s)	288, 332, 341, 623, 1309, 1738, 2670
HpySE526I	ACGT	5 cut(s)	890, 994, 1375, 1764, 2811
Hsp92I	GRCGYC	1 cut(s)	2033
HspAI	GCGC	3 cut(s)	372, 1938, 1940
Ksp22I	TGATCA	1 cut(s)	2749
Kzo9I	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
LmnI	GCTCC	7 cut(s)	139, 147, 210, 318, 1627, 2443, 2800
Lsp1109I	GCAGC	9 cut(s)	616, 638, 1106, 1320, 2011, 2231, 2414, 2606, 2812
LweI	GCATC	7 cut(s)	167, 604, 961, 1346, 1444, 1508, 1900
MaeI	CTAG	9 cut(s)	41, 477, 497, 905, 1742, 2337, 2382, 2454, 2657
MaeII	ACGT	5 cut(s)	890, 994, 1375, 1764, 2811
MaeIII	GTNAC	3 cut(s)	638, 1277, 1674
MalI	GATC	9 cut(s)	621, 696, 1073, 1173, 1213, 1947, 2048, 2112, 2751
MbiI	CCGCTC	1 cut(s)	97
MboI	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
MboII	GAAGA	8 cut(s)	54, 1120, 1250, 1422, 1584, 1805, 1850, 2639
MfeI	CAATTG	3 cut(s)	1224, 1494, 2207
MflI	RGATCY	3 cut(s)	1071, 1211, 2046
MhlI	GDGCHC	6 cut(s)	144, 315, 411, 1203, 2323, 2471
MlsI	TGGCCA	2 cut(s)	1761, 2351
MluNI	TGGCCA	2 cut(s)	1761, 2351
MmeI	TCCRAC	4 cut(s)	466, 794, 956, 1239
Mox20I	TGGCCA	2 cut(s)	1761, 2351
Mph1103I	ATGCAT	1 cut(s)	976
MroXI	GAANNNNTTC	1 cut(s)	461
MscI	TGGCCA	2 cut(s)	1761, 2351
MslI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
Msp20I	TGGCCA	2 cut(s)	1761, 2351
MspA1I	CMGCKG	3 cut(s)	629, 1505, 2024
MspCI	CTTAAG	1 cut(s)	2296
MspI	CCGG	4 cut(s)	48, 117, 415, 984
MspR9I	CCNGG	4 cut(s)	175, 234, 918, 2217
MunI	CAATTG	3 cut(s)	1224, 1494, 2207
Mva1269I	GAATGC	4 cut(s)	653, 1188, 1340, 2015
MvaI	CCWGG	4 cut(s)	175, 234, 918, 2217
MvnI	CGCG	5 cut(s)	183, 372, 454, 1938, 1940
MwoI	GCNNNNNNNGC	7 cut(s)	288, 332, 341, 623, 1309, 1738, 2670
NcoI	CCATGG	2 cut(s)	33, 514
NdeII	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
NheI	GCTAGC	1 cut(s)	2381
NlaIV	GGNNCC	8 cut(s)	179, 231, 922, 1213, 1873, 2214, 2469, 2802
NmeAIII	GCCGAG	1 cut(s)	345
NsiI	ATGCAT	1 cut(s)	976
NspI	RCATGY	1 cut(s)	916
OliI	CACNNNNGTG	1 cut(s)	527
PauI	GCGCGC	1 cut(s)	1938
PctI	GAATGC	4 cut(s)	653, 1188, 1340, 2015
PdmI	GAANNNNTTC	1 cut(s)	461
PfeI	GAWTC	7 cut(s)	536, 573, 963, 1034, 1616, 2193, 2764
PpuMI	RGGWCCY	3 cut(s)	177, 229, 920
PshAI	GACNNNNGTC	1 cut(s)	182
Psp124BI	GAGCTC	3 cut(s)	144, 315, 1203
Psp5II	RGGWCCY	3 cut(s)	177, 229, 920
Psp6I	CCWGG	4 cut(s)	173, 232, 916, 2215
PspFI	CCCAGC	2 cut(s)	1581, 2027
PspGI	CCWGG	4 cut(s)	173, 232, 916, 2215
PspN4I	GGNNCC	8 cut(s)	179, 231, 922, 1213, 1873, 2214, 2469, 2802
PspOMI	GGGCCC	1 cut(s)	2467
PspPI	GGNCC	7 cut(s)	37, 177, 229, 920, 1438, 2467, 2468
PspPPI	RGGWCCY	3 cut(s)	177, 229, 920
PstI	CTGCAG	2 cut(s)	628, 1338
PstNI	CAGNNNCTG	1 cut(s)	2027
PsuI	RGATCY	3 cut(s)	1071, 1211, 2046
PteI	GCGCGC	1 cut(s)	1938
PvuII	CAGCTG	3 cut(s)	629, 1505, 2024
RsaI	GTAC	3 cut(s)	533, 2567, 2832
RsaNI	GTAC	3 cut(s)	532, 2566, 2831
RseI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
SacI	GAGCTC	3 cut(s)	144, 315, 1203
Sau3AI	GATC	9 cut(s)	619, 694, 1071, 1171, 1211, 1945, 2046, 2110, 2749
Sau96I	GGNCC	7 cut(s)	37, 177, 229, 920, 1438, 2467, 2468
ScrFI	CCNGG	4 cut(s)	175, 234, 918, 2217
SduI	GDGCHC	6 cut(s)	144, 315, 411, 1203, 2323, 2471
SfaNI	GCATC	7 cut(s)	167, 604, 961, 1346, 1444, 1508, 1900
SfcI	CTRYAG	2 cut(s)	624, 1334
SinI	GGWCC	3 cut(s)	177, 229, 920
SmiMI	CAYNNNNRTG	5 cut(s)	513, 527, 1433, 1767, 1947
SmlI	CTYRAG	4 cut(s)	1202, 1586, 2296, 2518
SmoI	CTYRAG	4 cut(s)	1202, 1586, 2296, 2518
SspMI	CTAG	9 cut(s)	41, 477, 497, 905, 1742, 2337, 2382, 2454, 2657
SstI	GAGCTC	3 cut(s)	144, 315, 1203
StyD4I	CCNGG	4 cut(s)	173, 232, 916, 2215
StyI	CCWWGG	4 cut(s)	33, 514, 586, 958
TaaI	ACNGT	8 cut(s)	706, 1680, 1751, 1978, 2058, 2134, 2677, 2712
TaiI	ACGT	5 cut(s)	893, 997, 1378, 1767, 2814
TaqI	TCGA	4 cut(s)	763, 979, 1484, 2317
TauI	GCSGC	4 cut(s)	92, 372, 2673, 2746
TfiI	GAWTC	7 cut(s)	536, 573, 963, 1034, 1616, 2193, 2764
TscAI	CASTG	8 cut(s)	334, 529, 669, 1756, 1819, 2061, 2139, 2352
TseI	GCWGC	9 cut(s)	626, 629, 1094, 1333, 2024, 2244, 2402, 2594, 2825
TspDTI	ATGAA	7 cut(s)	17, 1283, 1623, 1806, 1853, 2238, 2658
TspGWI	ACGGA	4 cut(s)	293, 659, 1726, 2807
TspRI	CASTG	8 cut(s)	334, 529, 669, 1756, 1819, 2061, 2139, 2352
Vha464I	CTTAAG	1 cut(s)	2296
VneI	GTGCAC	1 cut(s)	407
VpaK11BI	GGWCC	3 cut(s)	177, 229, 920
XagI	CCTNNNNNAGG	1 cut(s)	2521
XapI	RAATTY	6 cut(s)	503, 598, 721, 1632, 2001, 2099
XceI	RCATGY	1 cut(s)	916
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2213, 2406
XmiI	GTMKAC	1 cut(s)	1468
XmnI	GAANNNNTTC	1 cut(s)	461
XspI	CTAG	9 cut(s)	41, 477, 497, 905, 1742, 2337, 2382, 2454, 2657
Zsp2I	ATGCAT	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.