AT3G12320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005747
Physical Location & Seq
Forward (+)
3923515 .. 3925502
1988 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12320.2

Sequence Viewer

Length: 810 bp
ATGGATTGTTATGCTGAAGAGCTTGTTGTGCCTAATTATCAAGAATCATCATCAGAGACATACCCATCTACTGGTATGTGGGGTGGATGGAGCATGAGCTCCCCTGAAGCAGCTGAGAAATGTTTCGATTACGATGGTTTCAATGGAGAAGGAATGATGTATAGTCAGATGAGTATGAGGACTAGTGAAGAAGAAGAAGAGTCTAAAAGATCAAAGGCTTTCTATGGTGCTTCTTCACTTCACGATTTCGAAGGCATCGAACAGATGGATGATATGTTCTTAAGTTCAATTTTGGAGGATGTTCCAGAGGATGATGGAGATGTTCACCGCGCGACCAGTAGCAATAACAGTGTCGGTTCTTCTTCTATGTATGGTGGTGGTAGGGAAGTCCCTATGTTCCATTGTCATGACATGTCTTTCAAGGAGGAAGCTCCATTTACAATCTCGGATCTGTCTGAAGAGAACATGTTAGATTCAAACTATGGGGATGAACTGTCTTCTGAAGAATTCGTGTTGCAGGATCTACAAAGGGCTTCACAAAAGTTGACTGATGAGACACGCAAATGTTTCCGGGATACTTTTTACCGACTTGCAAGGAGCTCACAAGATAAATCTGATTCAGTCAGCCCCAACTCGGAAGAGCTGCTTATGCAAACGTCTAGATATGATTATGGTGATGGAAACAGGTTTAGTAGAGAGGAAGAGATAGAATCTGAAACGAATTCAATCGATAGAGCTGTCGCAAACCTCACATTCAACAAAATGGAATCCAACATAAGCAATTTTCCTCTGTCGGAAAGAGTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007623 GO:0008150 GO:0048511
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

269

Amino Acids

30.55

Weight (kDa)

4.23

Isoelectric Point (pI)

79.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 71
AccII CGCG 2 cut(s) 330, 332
AciI CCGC 1 cut(s) 328
AclWI GGATC 2 cut(s) 456, 528
AcsI RAATTY 2 cut(s) 506, 721
AcuI CTGAAG 4 cut(s) 36, 126, 477, 522
AfaI GTAC 1 cut(s) 804
AfiI CCNNNNNNNGG 2 cut(s) 71, 634
AflII CTTAAG 1 cut(s) 280
AflIII ACRYGT 2 cut(s) 411, 465
AgsI TTSAA 6 cut(s) 142, 288, 421, 477, 726, 757
AhlI ACTAGT 1 cut(s) 182
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 7 cut(s) 22, 99, 113, 431, 600, 643, 737
AluI AGCT 7 cut(s) 22, 99, 113, 431, 600, 643, 737
Alw21I GWGCWC 2 cut(s) 101, 602
Alw26I GTCTC 2 cut(s) 50, 548
AlwI GGATC 2 cut(s) 456, 528
ApeKI GCWGC 2 cut(s) 110, 643
ApoI RAATTY 2 cut(s) 506, 721
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 332
AsuC2I CCSGG 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 317, 686
AsuII TTCGAA 1 cut(s) 249
BanII GRGCYC 2 cut(s) 101, 602
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 101, 602
BbvI GCAGC 2 cut(s) 122, 630
BccI CCATC 6 cut(s) 73, 81, 128, 259, 308, 671
BciVI GTATCC 1 cut(s) 568
BcnI CCSGG 1 cut(s) 572
BcoDI GTCTC 2 cut(s) 50, 548
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 2 cut(s) 183, 660
BfrI CTTAAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 568
BisI GCNGC 2 cut(s) 111, 644
BlsI GCNGC 2 cut(s) 112, 645
Bme1390I CCNGG 1 cut(s) 572
BmrFI CCNGG 1 cut(s) 572
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 572
Bsa29I ATCGAT 1 cut(s) 729
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 634
Bse1I ACTGG 2 cut(s) 76, 336
BseCI ATCGAT 1 cut(s) 729
BseGI GGATG 5 cut(s) 92, 274, 304, 316, 493
BseLI CCNNNNNNNGG 2 cut(s) 71, 634
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 2 cut(s) 76, 336
BseXI GCAGC 2 cut(s) 122, 630
Bsh1236I CGCG 2 cut(s) 330, 332
BshVI ATCGAT 1 cut(s) 729
BsiHKAI GWGCWC 2 cut(s) 101, 602
BsiSI CCGG 1 cut(s) 571
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 2 cut(s) 71, 634
BsmAI GTCTC 2 cut(s) 50, 548
BsmFI GGGAC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 101, 602
Bsp143I GATC 3 cut(s) 209, 448, 520
BspACI CCGC 1 cut(s) 328
BspCNI CTCAG 1 cut(s) 106
BspDI ATCGAT 1 cut(s) 729
BspFNI CGCG 2 cut(s) 330, 332
BspHI TCATGA 1 cut(s) 406
BspPI GGATC 2 cut(s) 456, 528
BspQI GCTCTTC 2 cut(s) 12, 633
BspT104I TTCGAA 1 cut(s) 249
BspTI CTTAAG 1 cut(s) 280
BsrI ACTGG 2 cut(s) 76, 336
BssMI GATC 3 cut(s) 209, 448, 520
Bst4CI ACNGT 3 cut(s) 350, 495, 807
Bst6I CTCTTC 5 cut(s) 12, 192, 453, 633, 696
BstAFI CTTAAG 1 cut(s) 280
BstBI TTCGAA 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 114
BstF5I GGATG 5 cut(s) 92, 274, 304, 316, 493
BstFNI CGCG 2 cut(s) 330, 332
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 212, 451, 523
BstMAI GTCTC 2 cut(s) 50, 548
BstMBI GATC 3 cut(s) 209, 448, 520
BstMWI GCNNNNNNNGC 2 cut(s) 28, 649
BstNSI RCATGY 2 cut(s) 415, 469
BstSCI CCNGG 1 cut(s) 570
BstUI CGCG 2 cut(s) 330, 332
BstV1I GCAGC 2 cut(s) 122, 630
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 2 cut(s) 448, 520
BstYI RGATCY 2 cut(s) 448, 520
Bsu15I ATCGAT 1 cut(s) 729
BsuI GTATCC 1 cut(s) 568
BsuTUI ATCGAT 1 cut(s) 729
BtsCI GGATG 5 cut(s) 92, 274, 304, 316, 493
BtsIMutI CAGTG 1 cut(s) 355
CciI TCATGA 1 cut(s) 406
CfoI GCGC 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 803
CviAII CATG 4 cut(s) 94, 407, 412, 466
CviQI GTAC 1 cut(s) 803
DdeI CTNAG 1 cut(s) 114
DpnI GATC 3 cut(s) 211, 450, 522
DpnII GATC 3 cut(s) 209, 448, 520
Eam1104I CTCTTC 5 cut(s) 12, 192, 453, 633, 696
EarI CTCTTC 5 cut(s) 12, 192, 453, 633, 696
Ecl136II GAGCTC 2 cut(s) 99, 600
Eco24I GRGCYC 2 cut(s) 101, 602
Eco53kI GAGCTC 2 cut(s) 99, 600
Eco57I CTGAAG 4 cut(s) 36, 126, 477, 522
EcoICRI GAGCTC 2 cut(s) 99, 600
EcoRI GAATTC 2 cut(s) 506, 721
EcoT38I GRGCYC 2 cut(s) 101, 602
FaeI CATG 4 cut(s) 97, 410, 415, 469
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 1 cut(s) 374
FatI CATG 4 cut(s) 93, 406, 411, 465
Fnu4HI GCNGC 2 cut(s) 111, 644
FokI GGATG 5 cut(s) 99, 281, 311, 323, 500
FriOI GRGCYC 2 cut(s) 101, 602
Fsp4HI GCNGC 2 cut(s) 111, 644
FspBI CTAG 2 cut(s) 183, 660
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 111, 644
HapII CCGG 1 cut(s) 571
HhaI GCGC 1 cut(s) 332
Hin1II CATG 4 cut(s) 97, 410, 415, 469
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 1 cut(s) 546
HindII GTYRAC 1 cut(s) 546
HinfI GANTC 6 cut(s) 44, 200, 473, 617, 710, 767
HpaII CCGG 1 cut(s) 571
HphI GGTGA 2 cut(s) 317, 686
Hpy166II GTNNAC 2 cut(s) 325, 546
Hpy188I TCNGA 9 cut(s) 55, 168, 448, 457, 502, 616, 637, 715, 796
Hpy188III TCNNGA 5 cut(s) 41, 242, 305, 407, 660
Hpy8I GTNNAC 2 cut(s) 325, 546
HpyAV CCTTC 2 cut(s) 143, 245
HpyCH4III ACNGT 3 cut(s) 350, 495, 807
HpyCH4IV ACGT 1 cut(s) 656
HpyCH4V TGCA 3 cut(s) 517, 593, 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 649
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 656
Hsp92II CATG 4 cut(s) 97, 410, 415, 469
HspAI GCGC 1 cut(s) 330
Kzo9I GATC 3 cut(s) 209, 448, 520
LguI GCTCTTC 2 cut(s) 12, 633
LmnI GCTCC 4 cut(s) 90, 104, 436, 597
LpnPI CCDG 7 cut(s) 57, 117, 318, 349, 503, 584, 670
Lsp1109I GCAGC 2 cut(s) 122, 630
LweI GCATC 1 cut(s) 264
MaeI CTAG 2 cut(s) 183, 660
MaeII ACGT 1 cut(s) 656
MalI GATC 3 cut(s) 211, 450, 522
MboI GATC 3 cut(s) 209, 448, 520
MflI RGATCY 2 cut(s) 448, 520
MhlI GDGCHC 2 cut(s) 101, 602
MluCI AATT 5 cut(s) 34, 288, 506, 721, 781
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 2 cut(s) 774, 795
MnlI CCTC 7 cut(s) 171, 289, 301, 418, 691, 758, 798
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 2 cut(s) 405, 562
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 280
MspI CCGG 1 cut(s) 571
MspR9I CCNGG 1 cut(s) 572
MvnI CGCG 2 cut(s) 330, 332
MwoI GCNNNNNNNGC 2 cut(s) 28, 649
NciI CCSGG 1 cut(s) 572
NdeII GATC 3 cut(s) 209, 448, 520
NlaIII CATG 4 cut(s) 97, 410, 415, 469
NspI RCATGY 2 cut(s) 415, 469
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 1 cut(s) 406
PciI ACATGT 2 cut(s) 411, 465
PciSI GCTCTTC 2 cut(s) 12, 633
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 5 cut(s) 44, 473, 617, 710, 767
PflMI CCANNNNNTGG 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 2 cut(s) 112, 645
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PscI ACATGT 2 cut(s) 411, 465
Psp124BI GAGCTC 2 cut(s) 101, 602
PsuI RGATCY 2 cut(s) 448, 520
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
RseI CAYNNNNRTG 2 cut(s) 405, 562
SacI GAGCTC 2 cut(s) 101, 602
SapI GCTCTTC 2 cut(s) 12, 633
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 2 cut(s) 111, 644
Sau3AI GATC 3 cut(s) 209, 448, 520
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 572
SduI GDGCHC 2 cut(s) 101, 602
SfaNI GCATC 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 249
SmiMI CAYNNNNRTG 2 cut(s) 405, 562
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 5 cut(s) 34, 288, 506, 721, 781
SsiI CCGC 1 cut(s) 328
SspMI CTAG 2 cut(s) 183, 660
SstI GAGCTC 2 cut(s) 101, 602
StyD4I CCNGG 1 cut(s) 570
TaaI ACNGT 3 cut(s) 350, 495, 807
TaiI ACGT 1 cut(s) 659
TaqI TCGA 4 cut(s) 126, 249, 258, 729
TasI AATT 5 cut(s) 34, 288, 506, 721, 781
TatI WGTACW 1 cut(s) 802
TfiI GAWTC 5 cut(s) 44, 473, 617, 710, 767
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 1 cut(s) 355
TseI GCWGC 2 cut(s) 110, 643
TspDTI ATGAA 1 cut(s) 504
TspRI CASTG 1 cut(s) 355
Van91I CCANNNNNTGG 1 cut(s) 71
Vha464I CTTAAG 1 cut(s) 280
XapI RAATTY 2 cut(s) 506, 721
XbaI TCTAGA 1 cut(s) 659
XceI RCATGY 2 cut(s) 415, 469
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 2 cut(s) 183, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.