Rh1AG083300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005747
Physical Location & Seq
Forward (+)
14245144 .. 14249414
4271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG083300.1

Sequence Viewer

Length: 1320 bp
ATGACCACTCGAGCCACGTTCCTCTACAGAAACTGGGTCTTGATCACTTTCTTAATTAGTCCATCACAAGAACAAGTCTCCATGGATTGGTATTTCGGGAATGGAATTGATGACCTTGTTGTTCCAAATGATGGAGGATCTGACAGGCTTCCATCACCAGATAGCTGGTCAAAATGGGGAATAAGTGCATCTGAGTACTTTCCTAATAAATGTTTTTCCATGTATCCACAATTCAGTAAAGAACTCAACTTCAGCAGTGGAAAAGGTTTATTTGATGAAGGTGAGATGGGAACTTCTGCTAATGACAGAGATCTATCCAGCAGTTCAAGTGTTTGTGAAGGATTGTCCGAGGGCTCTCTTCAGAAGACCTCACTTTCCTACAATCGGGCCTACAATCAACTTGAGGACGTAGCAGGACTACAACAGATGGATGACATTTTCTTGAGTTCCTTACTTGATGATCTTCCTGGGGCAGAAAATGTACATAAATCGTCGTTTTATTTTCGCTCTGATTCCAGTGGCATGTTAGCCAGTGACAGTATCTCGACAAGTATGAATTTGGAATCACAAAGTATTTCAAGCAGTGTTCACAGTGCTGGCAGCTCAAAGTATCTCAAAACTCATGCATTTTCACCGGAATTGGGTTCAGAGAAGGGGCATACCACTACTTCACAGTTCAGTCCATGCAACTCAGAGCAAAAAGACTCTCCACCAGTAAAGGCACGAACGGTTAGACTCATGTCTCCATCTGAGCAAGGGTGCATGCCTAGTGGTGTGGATGAGGAAACATCAGTTGAAGAATCTGTATTGCAGGAGCTGGAAATGGTGATGACACAGTTGACCAAAAAGACCCGAATCTGTTTTCGAGATGCTTTATACCGCCTTGCCGATAACTCAAAAGGAAATCCGGTTTCCCAGAGCCAAGATGGAGACCTTGCTGTTGAAGAACTGTCTTCATGGGCAGCACATGATGAAACAACGAGGTCTGGGAGCAAGGAAGGCACAGAATCAGAGACAAACACCATTGACCGCGCAATTGCAAACCTCATGTTTAACGAGATAGACTTCAATGCACAAGACCTTTCTGGAGGATCATCATCAGACTCCAAACATGAAATCAATGGAGTGTCTTGTCAGCAACAATCTGACAACCCCTGCCGGCTACAAATCTCTCATTCGCATCGCAATTCAGTTTTACCACATGATGCTGAGGTTCCTATACTTGGTCAAAGAGACGTGCATAAAAGTTTCTCAACAAGTAATGCTGGTGGAAAGAGGAAGACTCCAATGAGTGAGTTTGGGTCTGCAACTGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

439

Amino Acids

48.06

Weight (kDa)

4.74

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 87, 131
AccII CGCG 1 cut(s) 1032
AciI CCGC 2 cut(s) 880, 1030
AclWI GGATC 2 cut(s) 145, 1099
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 2 cut(s) 235, 344
AfaI GTAC 2 cut(s) 197, 483
AfiI CCNNNNNNNGG 5 cut(s) 87, 131, 384, 641, 1223
AgsI TTSAA 5 cut(s) 327, 579, 797, 944, 1069
AjiI CACGTC 1 cut(s) 1237
AjnI CCWGG 1 cut(s) 466
AluBI AGCT 3 cut(s) 165, 603, 817
AluI AGCT 3 cut(s) 165, 603, 817
Alw26I GTCTC 5 cut(s) 82, 747, 924, 1007, 1227
AlwI GGATC 2 cut(s) 145, 1099
AlwNI CAGNNNCTG 2 cut(s) 33, 817
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 1 cut(s) 387
ApeKI GCWGC 2 cut(s) 600, 962
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 1 cut(s) 1034
AspS9I GGNCC 1 cut(s) 387
AsuHPI GGTGA 4 cut(s) 147, 293, 624, 838
AvaI CYCGRG 1 cut(s) 9
BanII GRGCYC 1 cut(s) 356
BbsI GAAGAC 3 cut(s) 371, 945, 1286
BbvCI CCTCAGC 1 cut(s) 1209
BbvI GCAGC 2 cut(s) 612, 974
BccI CCATC 7 cut(s) 70, 125, 160, 280, 421, 754, 920
BciT130I CCWGG 1 cut(s) 468
BciVI GTATCC 1 cut(s) 234
BclI TGATCA 1 cut(s) 42
BcoDI GTCTC 5 cut(s) 82, 747, 924, 1007, 1227
BfaI CTAG 1 cut(s) 768
BfmI CTRYAG 1 cut(s) 25
BfuI GTATCC 1 cut(s) 234
BglII AGATCT 1 cut(s) 310
BisI GCNGC 2 cut(s) 601, 963
BlsI GCNGC 2 cut(s) 602, 964
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 1237
BmgT120I GGNCC 1 cut(s) 387
BmiI GGNNCC 1 cut(s) 1215
BmrFI CCNGG 1 cut(s) 468
BmrI ACTGGG 1 cut(s) 43
BmsI GCATC 4 cut(s) 197, 859, 1189, 1195
BmuI ACTGGG 1 cut(s) 43
BoxI GACNNNNGTC 1 cut(s) 739
BpiI GAAGAC 3 cut(s) 371, 945, 1286
BplI GAGNNNNNCTC 2 cut(s) 1267, 1299
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 1 cut(s) 1209
BpuEI CTTGAG 2 cut(s) 422, 463
BsaBI GATNNNNATC 1 cut(s) 1096
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 3 cut(s) 81, 348, 467
BsaWI WCCGGW 2 cut(s) 634, 907
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 131, 384, 641, 1223
Bse118I RCCGGY 1 cut(s) 1158
Bse1I ACTGG 4 cut(s) 38, 516, 531, 713
Bse8I GATNNNNATC 1 cut(s) 1096
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 81, 348, 467
BseGI GGATG 2 cut(s) 436, 784
BseJI GATNNNNATC 1 cut(s) 1096
BseLI CCNNNNNNNGG 5 cut(s) 87, 131, 384, 641, 1223
BseMII CTCAG 5 cut(s) 183, 705, 741, 1200, 1302
BseNI ACTGG 4 cut(s) 38, 516, 531, 713
BseXI GCAGC 2 cut(s) 612, 974
Bsh1236I CGCG 1 cut(s) 1032
BshFI GGCC 1 cut(s) 389
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 3 cut(s) 635, 908, 1159
BslI CCNNNNNNNGG 5 cut(s) 87, 131, 384, 641, 1223
BsmAI GTCTC 5 cut(s) 82, 747, 924, 1007, 1227
BsmBI CGTCTC 1 cut(s) 1227
BsnI GGCC 1 cut(s) 389
Bso31I GGTCTC 1 cut(s) 924
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 356
Bsp1407I TGTACA 1 cut(s) 481
Bsp143I GATC 5 cut(s) 42, 137, 310, 460, 1091
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 2 cut(s) 880, 1030
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 5 cut(s) 184, 704, 742, 1201, 1303
BspFNI CGCG 1 cut(s) 1032
BspLI GGNNCC 1 cut(s) 1215
BspPI GGATC 2 cut(s) 145, 1099
BspTNI GGTCTC 1 cut(s) 924
BsrFI RCCGGY 1 cut(s) 1158
BsrGI TGTACA 1 cut(s) 481
BsrI ACTGG 4 cut(s) 38, 516, 531, 713
BssAI RCCGGY 1 cut(s) 1158
BssECI CCNNGG 3 cut(s) 81, 348, 467
BssMI GATC 5 cut(s) 42, 137, 310, 460, 1091
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 6 cut(s) 539, 593, 675, 730, 837, 951
Bst6I CTCTTC 1 cut(s) 363
BstAUI TGTACA 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 598, 764, 1160
BstDEI CTNAG 5 cut(s) 192, 691, 750, 1209, 1311
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 436, 784
BstFNI CGCG 1 cut(s) 1032
BstHHI GCGC 1 cut(s) 1034
BstKTI GATC 5 cut(s) 45, 140, 313, 463, 1094
BstMAI GTCTC 5 cut(s) 82, 747, 924, 1007, 1227
BstMBI GATC 5 cut(s) 42, 137, 310, 460, 1091
BstMWI GCNNNNNNNGC 1 cut(s) 999
BstNI CCWGG 1 cut(s) 468
BstNSI RCATGY 2 cut(s) 526, 766
BstPAI GACNNNNGTC 1 cut(s) 739
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 25
BstUI CGCG 1 cut(s) 1032
BstV1I GCAGC 2 cut(s) 612, 974
BstV2I GAAGAC 3 cut(s) 371, 945, 1286
BstX2I RGATCY 2 cut(s) 137, 310
BstXI CCANNNNNNTGG 1 cut(s) 165
BstYI RGATCY 2 cut(s) 137, 310
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 1166
BtrI CACGTC 1 cut(s) 1237
BtsCI GGATG 2 cut(s) 436, 784
BtsI GCAGTG 2 cut(s) 262, 589
BtsIMutI CAGTG 5 cut(s) 262, 523, 538, 589, 598
Cac8I GCNNGC 3 cut(s) 598, 764, 1160
CaiI CAGNNNCTG 2 cut(s) 33, 817
CfoI GCGC 1 cut(s) 1034
Cfr10I RCCGGY 1 cut(s) 1158
Cfr13I GGNCC 1 cut(s) 387
Csp6I GTAC 2 cut(s) 196, 482
CviQI GTAC 2 cut(s) 196, 482
DdeI CTNAG 5 cut(s) 192, 691, 750, 1209, 1311
DpnI GATC 5 cut(s) 44, 139, 312, 462, 1093
DpnII GATC 5 cut(s) 42, 137, 310, 460, 1091
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 924
Eco57I CTGAAG 2 cut(s) 235, 344
Eco88I CYCGRG 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 1227
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 2 cut(s) 601, 963
FokI GGATG 2 cut(s) 443, 791
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 2 cut(s) 601, 963
FspBI CTAG 1 cut(s) 768
GlaI GCGC 1 cut(s) 1033
GluI GCNGC 2 cut(s) 601, 963
GsuI CTGGAG 1 cut(s) 1107
HaeIII GGCC 1 cut(s) 389
HapII CCGG 3 cut(s) 635, 908, 1159
HhaI GCGC 1 cut(s) 1034
Hin6I GCGC 1 cut(s) 1032
HinP1I GCGC 1 cut(s) 1032
HincII GTYRAC 1 cut(s) 840
HindII GTYRAC 1 cut(s) 840
HinfI GANTC 9 cut(s) 512, 563, 704, 735, 800, 855, 1007, 1103, 1282
HpaII CCGG 3 cut(s) 635, 908, 1159
HphI GGTGA 4 cut(s) 147, 293, 624, 838
Hpy166II GTNNAC 2 cut(s) 589, 840
Hpy188III TCNNGA 6 cut(s) 40, 97, 442, 544, 866, 1086
Hpy8I GTNNAC 2 cut(s) 589, 840
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 4 cut(s) 272, 332, 646, 992
HpyCH4III ACNGT 6 cut(s) 539, 593, 675, 730, 837, 951
HpyCH4IV ACGT 3 cut(s) 17, 408, 1236
HpyCH4V TGCA 9 cut(s) 188, 626, 687, 762, 811, 1040, 1073, 1240, 1307
HpyF10VI GCNNNNNNNGC 1 cut(s) 999
HpyF3I CTNAG 5 cut(s) 192, 691, 750, 1209, 1311
HpySE526I ACGT 3 cut(s) 17, 408, 1236
HspAI GCGC 1 cut(s) 1032
KroI GCCGGC 1 cut(s) 1158
KroNI GCCGGC 1 cut(s) 1160
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 5 cut(s) 42, 137, 310, 460, 1091
LmnI GCTCC 2 cut(s) 814, 990
Lsp1109I GCAGC 2 cut(s) 612, 974
LweI GCATC 4 cut(s) 197, 859, 1189, 1195
MaeI CTAG 1 cut(s) 768
MaeII ACGT 3 cut(s) 17, 408, 1236
MaeIII GTNAC 1 cut(s) 533
MalI GATC 5 cut(s) 44, 139, 312, 462, 1093
MboI GATC 5 cut(s) 42, 137, 310, 460, 1091
MboII GAAGA 7 cut(s) 350, 376, 455, 809, 945, 956, 1291
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 2 cut(s) 137, 310
MhlI GDGCHC 1 cut(s) 356
MluCI AATT 7 cut(s) 54, 105, 230, 556, 638, 1035, 1186
MlyI GAGTC 4 cut(s) 698, 729, 1097, 1276
Mph1103I ATGCAT 1 cut(s) 628
MroNI GCCGGC 1 cut(s) 1158
MseI TTAA 2 cut(s) 53, 1053
MspI CCGG 3 cut(s) 635, 908, 1159
MspR9I CCNGG 1 cut(s) 468
MunI CAATTG 1 cut(s) 1035
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 1 cut(s) 1032
MwoI GCNNNNNNNGC 1 cut(s) 999
NaeI GCCGGC 1 cut(s) 1160
NcoI CCATGG 1 cut(s) 81
NdeII GATC 5 cut(s) 42, 137, 310, 460, 1091
NgoMIV GCCGGC 1 cut(s) 1158
NlaIV GGNNCC 1 cut(s) 1215
NmuCI GTSAC 1 cut(s) 533
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 2 cut(s) 526, 766
PaeI GCATGC 1 cut(s) 766
PaeR7I CTCGAG 1 cut(s) 9
PdiI GCCGGC 1 cut(s) 1160
PfeI GAWTC 5 cut(s) 512, 563, 800, 855, 1007
PflMI CCANNNNNTGG 2 cut(s) 87, 131
PkrI GCNGC 2 cut(s) 602, 964
PleI GAGTC 4 cut(s) 698, 729, 1097, 1276
PpsI GAGTC 4 cut(s) 698, 729, 1097, 1276
PshAI GACNNNNGTC 1 cut(s) 739
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 1215
PspPI GGNCC 1 cut(s) 387
PspXI VCTCGAGB 1 cut(s) 9
PstNI CAGNNNCTG 2 cut(s) 33, 817
PsuI RGATCY 2 cut(s) 137, 310
RsaI GTAC 2 cut(s) 197, 483
RsaNI GTAC 2 cut(s) 196, 482
SaqAI TTAA 2 cut(s) 53, 1053
SatI GCNGC 2 cut(s) 601, 963
Sau3AI GATC 5 cut(s) 42, 137, 310, 460, 1091
Sau96I GGNCC 1 cut(s) 387
ScaI AGTACT 1 cut(s) 197
SchI GAGTC 4 cut(s) 698, 729, 1097, 1276
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 1 cut(s) 356
SfaNI GCATC 4 cut(s) 197, 859, 1189, 1195
SfcI CTRYAG 1 cut(s) 25
Sfr274I CTCGAG 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 9
SmlI CTYRAG 3 cut(s) 9, 401, 442
SmoI CTYRAG 3 cut(s) 9, 401, 442
SphI GCATGC 1 cut(s) 766
Sse9I AATT 7 cut(s) 54, 105, 230, 556, 638, 1035, 1186
SsiI CCGC 2 cut(s) 880, 1030
SspMI CTAG 1 cut(s) 768
StyD4I CCNGG 1 cut(s) 466
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 6 cut(s) 539, 593, 675, 730, 837, 951
TaiI ACGT 3 cut(s) 20, 411, 1239
TaqI TCGA 3 cut(s) 10, 545, 865
TasI AATT 7 cut(s) 54, 105, 230, 556, 638, 1035, 1186
TatI WGTACW 2 cut(s) 195, 481
TfiI GAWTC 5 cut(s) 512, 563, 800, 855, 1007
Tru1I TTAA 2 cut(s) 53, 1053
Tru9I TTAA 2 cut(s) 53, 1053
TscAI CASTG 5 cut(s) 262, 523, 538, 589, 598
TseFI GTSAC 1 cut(s) 533
TseI GCWGC 2 cut(s) 600, 962
Tsp45I GTSAC 1 cut(s) 533
TspDTI ATGAA 5 cut(s) 291, 569, 945, 987, 1128
TspRI CASTG 5 cut(s) 262, 523, 538, 589, 598
Van91I CCANNNNNTGG 2 cut(s) 87, 131
XapI RAATTY 1 cut(s) 556
XceI RCATGY 2 cut(s) 526, 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XhoI CTCGAG 1 cut(s) 9
XspI CTAG 1 cut(s) 768
ZrmI AGTACT 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.