pycom02g23690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005747
Physical Location & Seq
Reverse (-)
21840035 .. 21842908
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23690.3

Sequence Viewer

Length: 1053 bp
ATGGAAACTTCTGTTAATGAAAAAGATCTGTCGAGCAGTTCAAGTGCATGTGAAGGATTGTCCGAGGCCTCTCTTCAGCAGACCACTCTTTCATACAATCGGGCTAATAATCAACTTGAAGACCTAGCAGGACTCGAACAGATGGATGACATTTTCCTGAGTTCCTTACTTGACGATCTTCCTGGGGCAGAAAACGCACACAAATCATTCTCTTTTTGTTCTGATTCCAATGGCTTGTTACCCACTGATAGTATCTCGACAAGCACGAGTTTGGAAGCATCAAGTATCTCGAGCAGTGTGCGCAGTGCAGGAAATTCAATGTATCTTCAAACTCATGCATTTTCACCAACTGTGGGTTCTGATAAACGAGATGCCACTACTTCACAGTTTAATCAGTGCAACTCGGAGAACGAAAATTTCCTTCCAGTAAAGACAGCAAGAGTTTTTGCCCCACCTGAGCAAAGCAACAGGAATGGACCCATTGGTGAGGAGTCATCAGTTGAAGAGTCTGTATTACATGAACTGGAAATGGTGATGAAACTGTTGAATGAGAAGACCCGAATTTGCTTTCGAGATTCCTTGTACCGTCTTGCAAACAACTCAAACGGAAATCACACTACACAGAGCCAAGATGGGGCTGAAGCAATCGAAAATGAAAAACCCTTATCATGGACAGCCCAGGATGAAACAGTCAGGTCAGGGAGCAATAAAGGGACGGAATCAGAGACGAACGCCATTGACAGAGCAATCGCAAACCTCATGTTTAATGAGATGGACTTTAATGCACAGGACCTTTCTGGAGGCGCATCATTAGACTCCAAACAAGAAGTCAATGGAGCGACTGGGCAACAAGCTGACGACGCTTACCGGCCACAAATCTCTCATTCACATATCCCCTCACTTGTACCACAAGATGCTGAAGTTCCAACACTAGGTGAAATAAACATGGATGTGGCGACAGATGATGAAATGCACATCAGTTTCTCGTCATGTAATGCTGGTGGGAAGCGAAAAGCTCCAATGAGGGAGTTCGCGTCTACAACTGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

37.9

Weight (kDa)

4.41

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 302
AccI GTMKAC 1 cut(s) 1037
AccII CGCG 1 cut(s) 1034
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 3 cut(s) 313, 415, 561
AcuI CTGAAG 3 cut(s) 59, 660, 939
AdeI CACNNNGTG 1 cut(s) 935
AfaI GTAC 2 cut(s) 584, 906
AfiI CCNNNNNNNGG 4 cut(s) 353, 634, 669, 932
AgsI TTSAA 6 cut(s) 42, 119, 318, 329, 503, 547
AjnI CCWGG 2 cut(s) 181, 678
AjuI GAANNNNNNNTTGG 2 cut(s) 340, 372
AluBI AGCT 2 cut(s) 854, 1016
AluI AGCT 2 cut(s) 854, 1016
Alw26I GTCTC 1 cut(s) 719
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 2 cut(s) 66, 869
ApoI RAATTY 3 cut(s) 313, 415, 561
ArsI GACNNNNNNTTYG 2 cut(s) 813, 845
AspLEI GCGC 2 cut(s) 303, 806
AspS9I GGNCC 2 cut(s) 476, 790
AsuHPI GGTGA 4 cut(s) 336, 497, 544, 947
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 2 cut(s) 476, 790
BauI CACGAG 1 cut(s) 265
BbsI GAAGAC 2 cut(s) 126, 560
BccI CCATC 3 cut(s) 136, 626, 766
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 2 cut(s) 183, 680
BcoDI GTCTC 1 cut(s) 719
BfaI CTAG 2 cut(s) 125, 932
BglII AGATCT 1 cut(s) 25
Bme1390I CCNGG 2 cut(s) 183, 680
Bme18I GGWCC 2 cut(s) 476, 790
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 2 cut(s) 476, 790
BmiI GGNNCC 1 cut(s) 478
BmrFI CCNGG 2 cut(s) 183, 680
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 287, 361, 815, 904
BmuI ACTGGG 1 cut(s) 852
BpiI GAAGAC 2 cut(s) 126, 560
BpmI CTGGAG 1 cut(s) 819
Bpu10I CCTNAGC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 63, 182, 678
BsaXI ACNNNNNCTCC 2 cut(s) 1019, 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 353, 634, 669, 932
Bse118I RCCGGY 1 cut(s) 867
Bse1I ACTGG 3 cut(s) 425, 528, 847
BseBI CCWGG 2 cut(s) 183, 680
BseDI CCNNGG 3 cut(s) 63, 182, 678
BseGI GGATG 3 cut(s) 151, 688, 955
BseLI CCNNNNNNNGG 4 cut(s) 353, 634, 669, 932
BseMII CTCAG 3 cut(s) 149, 447, 1035
BseNI ACTGG 3 cut(s) 425, 528, 847
BseRI GAGGAG 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 327
Bsh1236I CGCG 1 cut(s) 1034
BshFI GGCC 2 cut(s) 68, 871
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 1 cut(s) 868
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 4 cut(s) 353, 634, 669, 932
BsmAI GTCTC 1 cut(s) 719
BsmBI CGTCTC 1 cut(s) 719
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 2 cut(s) 68, 871
BsoBI CYCGRG 1 cut(s) 289
Bsp143I GATC 2 cut(s) 25, 175
BspANI GGCC 2 cut(s) 68, 871
BspCNI CTCAG 3 cut(s) 150, 448, 1036
BspFNI CGCG 1 cut(s) 1034
BspLI GGNNCC 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 867
BsrI ACTGG 3 cut(s) 425, 528, 847
BssAI RCCGGY 1 cut(s) 867
BssECI CCNNGG 3 cut(s) 63, 182, 678
BssMI GATC 2 cut(s) 25, 175
BssSI CACGAG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 2 cut(s) 183, 680
Bst4CI ACNGT 5 cut(s) 352, 387, 543, 587, 691
Bst6I CTCTTC 2 cut(s) 78, 498
BstDEI CTNAG 3 cut(s) 158, 456, 1044
BstF5I GGATG 3 cut(s) 151, 688, 955
BstFNI CGCG 1 cut(s) 1034
BstHHI GCGC 2 cut(s) 303, 806
BstKTI GATC 2 cut(s) 28, 178
BstMAI GTCTC 1 cut(s) 719
BstMBI GATC 2 cut(s) 25, 175
BstMWI GCNNNNNNNGC 3 cut(s) 194, 300, 860
BstNI CCWGG 2 cut(s) 183, 680
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 181, 678
BstUI CGCG 1 cut(s) 1034
BstV2I GAAGAC 2 cut(s) 126, 560
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BsuRI GGCC 2 cut(s) 68, 871
BtsCI GGATG 3 cut(s) 151, 688, 955
BtsI GCAGTG 2 cut(s) 301, 310
BtsIMutI CAGTG 4 cut(s) 243, 301, 310, 401
CfoI GCGC 2 cut(s) 303, 806
Cfr10I RCCGGY 1 cut(s) 867
Cfr13I GGNCC 2 cut(s) 476, 790
CseI GACGC 2 cut(s) 869, 1023
Csp6I GTAC 2 cut(s) 583, 905
CviAII CATG 7 cut(s) 48, 335, 518, 669, 760, 946, 990
CviJI RGCY 9 cut(s) 68, 104, 234, 627, 638, 677, 854, 871, 1016
CviKI_1 RGCY 9 cut(s) 68, 104, 234, 627, 638, 677, 854, 871, 1016
CviQI GTAC 2 cut(s) 583, 905
DdeI CTNAG 3 cut(s) 158, 456, 1044
DpnI GATC 2 cut(s) 27, 177
DpnII GATC 2 cut(s) 25, 175
DraIII CACNNNGTG 1 cut(s) 935
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 2 cut(s) 78, 498
EarI CTCTTC 2 cut(s) 78, 498
Eco147I AGGCCT 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 476, 790
Eco57I CTGAAG 3 cut(s) 59, 660, 939
Eco88I CYCGRG 1 cut(s) 289
EcoO109I RGGNCCY 1 cut(s) 790
EcoRII CCWGG 2 cut(s) 181, 678
EcoT22I ATGCAT 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 719
FaeI CATG 7 cut(s) 51, 338, 521, 672, 763, 949, 993
FaiI YATR 9 cut(s) 49, 94, 336, 519, 670, 761, 891, 947, 991
FaqI GGGAC 1 cut(s) 727
FatI CATG 7 cut(s) 47, 334, 517, 668, 759, 945, 989
FblI GTMKAC 1 cut(s) 1037
FokI GGATG 3 cut(s) 158, 695, 962
FspBI CTAG 2 cut(s) 125, 932
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 2 cut(s) 302, 805
GsuI CTGGAG 1 cut(s) 819
HaeIII GGCC 2 cut(s) 68, 871
HapII CCGG 1 cut(s) 868
HgaI GACGC 2 cut(s) 869, 1023
HhaI GCGC 2 cut(s) 303, 806
Hin1II CATG 7 cut(s) 51, 338, 521, 672, 763, 949, 993
Hin6I GCGC 2 cut(s) 301, 804
HinP1I GCGC 2 cut(s) 301, 804
HinfI GANTC 7 cut(s) 132, 224, 491, 506, 575, 719, 815
HpaII CCGG 1 cut(s) 868
HphI GGTGA 4 cut(s) 336, 497, 544, 947
Hpy166II GTNNAC 1 cut(s) 1038
Hpy188I TCNGA 5 cut(s) 64, 223, 361, 406, 724
Hpy188III TCNNGA 5 cut(s) 157, 256, 289, 572, 798
Hpy8I GTNNAC 1 cut(s) 1038
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 2 cut(s) 47, 431
HpyCH4III ACNGT 5 cut(s) 352, 387, 543, 587, 691
HpyCH4V TGCA 7 cut(s) 47, 308, 338, 399, 593, 785, 973
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 300, 860
HpyF3I CTNAG 3 cut(s) 158, 456, 1044
Hsp92II CATG 7 cut(s) 51, 338, 521, 672, 763, 949, 993
HspAI GCGC 2 cut(s) 301, 804
Kzo9I GATC 2 cut(s) 25, 175
LmnI GCTCC 3 cut(s) 702, 836, 1021
LweI GCATC 4 cut(s) 287, 361, 815, 904
MaeI CTAG 2 cut(s) 125, 932
MaeIII GTNAC 1 cut(s) 237
MalI GATC 2 cut(s) 27, 177
MboI GATC 2 cut(s) 25, 175
MboII GAAGA 6 cut(s) 65, 131, 170, 317, 515, 565
MflI RGATCY 1 cut(s) 25
MluCI AATT 3 cut(s) 313, 415, 561
MlyI GAGTC 4 cut(s) 126, 500, 515, 809
MmeI TCCRAC 1 cut(s) 950
MnlI CCTC 7 cut(s) 58, 79, 481, 767, 794, 907, 1017
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 4 cut(s) 15, 390, 765, 780
MslI CAYNNNNRTG 1 cut(s) 950
MspI CCGG 1 cut(s) 868
MspR9I CCNGG 2 cut(s) 183, 680
MvaI CCWGG 2 cut(s) 183, 680
MvnI CGCG 1 cut(s) 1034
MwoI GCNNNNNNNGC 3 cut(s) 194, 300, 860
NdeII GATC 2 cut(s) 25, 175
NlaIII CATG 7 cut(s) 51, 338, 521, 672, 763, 949, 993
NlaIV GGNNCC 1 cut(s) 478
NsbI TGCGCA 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 289
PceI AGGCCT 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PfeI GAWTC 3 cut(s) 224, 575, 719
PleI GAGTC 4 cut(s) 126, 499, 514, 809
PpsI GAGTC 4 cut(s) 126, 499, 514, 809
PpuMI RGGWCCY 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 181, 678
PspGI CCWGG 2 cut(s) 181, 678
PspN4I GGNNCC 1 cut(s) 478
PspPI GGNCC 2 cut(s) 476, 790
PspPPI RGGWCCY 1 cut(s) 790
PsuI RGATCY 1 cut(s) 25
RsaI GTAC 2 cut(s) 584, 906
RsaNI GTAC 2 cut(s) 583, 905
RseI CAYNNNNRTG 1 cut(s) 950
SaqAI TTAA 4 cut(s) 15, 390, 765, 780
Sau3AI GATC 2 cut(s) 25, 175
Sau96I GGNCC 2 cut(s) 476, 790
SchI GAGTC 4 cut(s) 126, 500, 515, 809
ScrFI CCNGG 2 cut(s) 183, 680
SetI ASST 8 cut(s) 126, 457, 698, 759, 795, 856, 937, 1018
SfaNI GCATC 4 cut(s) 287, 361, 815, 904
Sfr274I CTCGAG 1 cut(s) 289
SinI GGWCC 2 cut(s) 476, 790
SlaI CTCGAG 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 3 cut(s) 313, 415, 561
SseBI AGGCCT 1 cut(s) 68
SspMI CTAG 2 cut(s) 125, 932
StuI AGGCCT 1 cut(s) 68
StyD4I CCNGG 2 cut(s) 181, 678
TaaI ACNGT 5 cut(s) 352, 387, 543, 587, 691
TaqI TCGA 6 cut(s) 32, 135, 257, 290, 571, 648
TasI AATT 3 cut(s) 313, 415, 561
TfiI GAWTC 3 cut(s) 224, 575, 719
Tru1I TTAA 4 cut(s) 15, 390, 765, 780
Tru9I TTAA 4 cut(s) 15, 390, 765, 780
TscAI CASTG 4 cut(s) 250, 301, 310, 401
TspDTI ATGAA 7 cut(s) 33, 81, 534, 551, 669, 699, 981
TspGWI ACGGA 2 cut(s) 621, 731
TspRI CASTG 4 cut(s) 250, 301, 310, 401
VpaK11BI GGWCC 2 cut(s) 476, 790
XapI RAATTY 3 cut(s) 313, 415, 561
XceI RCATGY 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 1037
XspI CTAG 2 cut(s) 125, 932
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.