Rorug01G0066400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005747
Physical Location & Seq
Forward (+)
10747567 .. 10748166
600 bp
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UTR
Exon/CDS
Intron
Rorug01G0066400.1

Sequence Viewer

Length: 402 bp
ATGCTCGATTCTACCCGAATCACCTGCTCCGAAATCCGTCACATTCTCACCCGGTGGGCTATTGTTCAAACCCGGAGGAAGATGATCGACTTCACCAAAGGACAGGTTGACTATATTCTAGGAAGCAACCCATTGGGAATGTCATACATGGTGGGATATGGTTCAAAGTTTCCAAAGAGAATACACCACCGCGCTTCAGTGCTACCTTCTATGGATCAGCAGCCGGGCAACATTGAATGTCACGCAGGAACGCCATATTTCGAGACTCAAGCTCCAAATCAGAACTTGCTAATAGGCGCGATTGTGGGAGGACCTGCTGAGAACGATTCTTTCGAAGACTCTCGGTACAATGTGTACAATGACGAGAATGGGCCTGGTTTAGCCGGAAAACTAGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

133

Amino Acids

14.84

Weight (kDa)

6.9

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 23 - 122 4.5e-29 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 32
Acc36I ACCTGC 2 cut(s) 32, 322
AccII CGCG 2 cut(s) 192, 299
AciI CCGC 1 cut(s) 190
AclWI GGATC 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 54
AfaI GTAC 2 cut(s) 347, 356
AgsI TTSAA 3 cut(s) 68, 165, 236
AjnI CCWGG 1 cut(s) 373
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 220
AspLEI GCGC 2 cut(s) 194, 299
AspS9I GGNCC 2 cut(s) 311, 371
AsuC2I CCSGG 3 cut(s) 52, 73, 225
AsuHPI GGTGA 3 cut(s) 13, 40, 85
AsuII TTCGAA 1 cut(s) 333
AvaII GGWCC 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 232
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 375
BcnI CCSGG 3 cut(s) 52, 73, 225
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 2 cut(s) 119, 392
BfuAI ACCTGC 2 cut(s) 32, 322
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
Bme1390I CCNGG 4 cut(s) 52, 73, 225, 375
Bme18I GGWCC 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 311, 371
BmrFI CCNGG 4 cut(s) 52, 73, 225, 375
BpiI GAAGAC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 252
BpuMI CCSGG 3 cut(s) 52, 73, 225
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
BseBI CCWGG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 232
Bsh1236I CGCG 2 cut(s) 192, 299
BshFI GGCC 1 cut(s) 373
BsiSI CCGG 4 cut(s) 52, 73, 224, 384
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 1 cut(s) 373
Bsp119I TTCGAA 1 cut(s) 333
Bsp1407I TGTACA 1 cut(s) 354
Bsp143I GATC 2 cut(s) 84, 214
BspACI CCGC 1 cut(s) 190
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 2 cut(s) 192, 299
BspMI ACCTGC 2 cut(s) 32, 322
BspPI GGATC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 333
BsrGI TGTACA 1 cut(s) 354
BssMI GATC 2 cut(s) 84, 214
Bst2UI CCWGG 1 cut(s) 375
BstAUI TGTACA 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 318
BstFNI CGCG 2 cut(s) 192, 299
BstHHI GCGC 2 cut(s) 194, 299
BstKTI GATC 2 cut(s) 87, 217
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 2 cut(s) 84, 214
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 4 cut(s) 50, 71, 223, 373
BstUI CGCG 2 cut(s) 192, 299
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 1 cut(s) 342
BsuRI GGCC 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 204
BveI ACCTGC 2 cut(s) 32, 322
CfoI GCGC 2 cut(s) 194, 299
Cfr13I GGNCC 2 cut(s) 311, 371
Csp6I GTAC 2 cut(s) 346, 355
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 59, 223, 272, 373, 383
CviKI_1 RGCY 5 cut(s) 59, 223, 272, 373, 383
CviQI GTAC 2 cut(s) 346, 355
DdeI CTNAG 1 cut(s) 318
DpnI GATC 2 cut(s) 86, 216
DpnII GATC 2 cut(s) 84, 214
DraIII CACNNNGTG 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 373
FaeI CATG 1 cut(s) 151
FaiI YATR 6 cut(s) 114, 145, 149, 159, 212, 256
FatI CATG 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 221
FspBI CTAG 2 cut(s) 119, 392
GlaI GCGC 2 cut(s) 193, 298
GluI GCNGC 1 cut(s) 221
HaeIII GGCC 1 cut(s) 373
HapII CCGG 4 cut(s) 52, 73, 224, 384
HhaI GCGC 2 cut(s) 194, 299
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 192, 297
HinP1I GCGC 2 cut(s) 192, 297
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 5 cut(s) 8, 18, 265, 326, 338
HpaII CCGG 4 cut(s) 52, 73, 224, 384
HphI GGTGA 3 cut(s) 13, 40, 85
Hpy166II GTNNAC 2 cut(s) 109, 355
Hpy188I TCNGA 2 cut(s) 31, 282
Hpy188III TCNNGA 1 cut(s) 262
Hpy8I GTNNAC 2 cut(s) 109, 355
HpyAV CCTTC 1 cut(s) 216
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 192, 297
Kzo9I GATC 2 cut(s) 84, 214
LmnI GCTCC 2 cut(s) 32, 277
Lsp1109I GCAGC 1 cut(s) 232
MaeI CTAG 2 cut(s) 119, 392
MaeIII GTNAC 2 cut(s) 38, 239
MalI GATC 2 cut(s) 86, 216
MboI GATC 2 cut(s) 84, 214
MboII GAAGA 2 cut(s) 91, 347
MlyI GAGTC 2 cut(s) 259, 332
MnlI CCTC 2 cut(s) 69, 302
MspI CCGG 4 cut(s) 52, 73, 224, 384
MspR9I CCNGG 4 cut(s) 52, 73, 225, 375
MvaI CCWGG 1 cut(s) 375
MvnI CGCG 2 cut(s) 192, 299
NciI CCSGG 3 cut(s) 52, 73, 225
NdeII GATC 2 cut(s) 84, 214
NlaIII CATG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 38, 239
NspV TTCGAA 1 cut(s) 333
PaqCI CACCTGC 1 cut(s) 32
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 3 cut(s) 8, 18, 326
PkrI GCNGC 1 cut(s) 222
PleI GAGTC 2 cut(s) 259, 332
PpsI GAGTC 2 cut(s) 259, 332
PpuMI RGGWCCY 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspPI GGNCC 2 cut(s) 311, 371
PspPPI RGGWCCY 1 cut(s) 311
RsaI GTAC 2 cut(s) 347, 356
RsaNI GTAC 2 cut(s) 346, 355
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 2 cut(s) 84, 214
Sau96I GGNCC 2 cut(s) 311, 371
SchI GAGTC 2 cut(s) 259, 332
ScrFI CCNGG 4 cut(s) 52, 73, 225, 375
SetI ASST 5 cut(s) 26, 108, 208, 274, 316
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SsiI CCGC 1 cut(s) 190
SspMI CTAG 2 cut(s) 119, 392
StyD4I CCNGG 4 cut(s) 50, 71, 223, 373
TaqI TCGA 4 cut(s) 6, 87, 261, 333
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 3 cut(s) 8, 18, 326
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 2 cut(s) 38, 239
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 2 cut(s) 38, 239
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 311
XspI CTAG 2 cut(s) 119, 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.