pycom07g03150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005747
Physical Location & Seq
Reverse (-)
2599508 .. 2601654
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03150.2

Sequence Viewer

Length: 552 bp
ATGTATCCACAATTCATTAGAGAGCTCAACTTCAGTGGTAACAGTTTGTGTGGTGAAGCCATGATGGAAACTTCTGTTAATGAAAAAGATCCGTCGAGCAGTTCAAGTGCATGTGAAGGATTGTCCGAGGCCTCTCTTCAGCAGACTACTCTTTCAGACAATCGGGCCTATAATCAACTTGAAGACCTAGCAGGACTCGACCAGATGGATGACATTTTCTTGAGTTCCTTACTTGGTGATCTTCCTGGGTCAGAAAATGCACACAAATCATTCTATTTTTGTTCTGATTCCAATGGCATGTTACCTACTGATTGTATCTCTACAAGCATGAGTTTGGAATCACCAAGTATCTCGAGCAGTGTGCACAGTGCAGGAAATTCAATGTGTCTTCAAACTCATGCCTTGTCACCAACTGTGGGTTCAGATAAAGGGGATTTCACCACTTCACAGTTTATTCAATGCAACTCGGAGCACAAAGATTGTCTTCCAGTAAAGTTAACAAGAACTTTTTTTCCCTCCCTCAGAAAGCAGCATGGATGGACTTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.05

Weight (kDa)

4.57

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 83
AcsI RAATTY 1 cut(s) 376
AcuI CTGAAG 2 cut(s) 16, 122
AfiI CCNNNNNNNGG 1 cut(s) 416
AgsI TTSAA 5 cut(s) 105, 182, 381, 392, 458
AjnI CCWGG 1 cut(s) 244
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AloI GAACNNNNNNTCC 2 cut(s) 496, 528
AluBI AGCT 1 cut(s) 25
AluI AGCT 1 cut(s) 25
Alw21I GWGCWC 3 cut(s) 27, 366, 474
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 2 cut(s) 129, 165
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 1 cut(s) 529
ApoI RAATTY 1 cut(s) 376
AspS9I GGNCC 1 cut(s) 165
AsuHPI GGTGA 5 cut(s) 65, 248, 333, 399, 430
AvaI CYCGRG 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 366
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 3 cut(s) 189, 380, 476
Bbv12I GWGCWC 3 cut(s) 27, 366, 474
BbvI GCAGC 1 cut(s) 541
BccI CCATC 3 cut(s) 58, 199, 531
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 1 cut(s) 246
BciVI GTATCC 1 cut(s) 15
BfaI CTAG 1 cut(s) 188
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 246
BpiI GAAGAC 3 cut(s) 189, 380, 476
BpuEI CTTGAG 1 cut(s) 241
BsaJI CCNNGG 2 cut(s) 126, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 416
Bse1I ACTGG 1 cut(s) 488
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 126, 245
BseGI GGATG 2 cut(s) 214, 542
BseLI CCNNNNNNNGG 1 cut(s) 416
BseMII CTCAG 1 cut(s) 535
BseNI ACTGG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 1 cut(s) 541
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 2 cut(s) 131, 167
BsiHKAI GWGCWC 3 cut(s) 27, 366, 474
BsiHKCI CYCGRG 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 416
BsnI GGCC 2 cut(s) 131, 167
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 27, 366, 474
Bsp143I GATC 2 cut(s) 88, 238
BspANI GGCC 2 cut(s) 131, 167
BspCNI CTCAG 1 cut(s) 534
BspPI GGATC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 2 cut(s) 126, 245
BssMI GATC 2 cut(s) 88, 238
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 4 cut(s) 44, 368, 415, 450
Bst6I CTCTTC 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 521
BstF5I GGATG 2 cut(s) 214, 542
BstKTI GATC 2 cut(s) 91, 241
BstMBI GATC 2 cut(s) 88, 238
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 114, 301
BstSCI CCNGG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 3 cut(s) 189, 380, 476
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 2 cut(s) 131, 167
BtsCI GGATG 2 cut(s) 214, 542
BtsI GCAGTG 1 cut(s) 364
BtsIMutI CAGTG 3 cut(s) 40, 364, 373
Cfr13I GGNCC 1 cut(s) 165
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 6 cut(s) 61, 111, 298, 328, 398, 533
CviJI RGCY 4 cut(s) 25, 59, 131, 167
CviKI_1 RGCY 4 cut(s) 25, 59, 131, 167
DdeI CTNAG 1 cut(s) 521
DpnI GATC 2 cut(s) 90, 240
DpnII GATC 2 cut(s) 88, 238
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 2 cut(s) 16, 122
Eco88I CYCGRG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 6 cut(s) 64, 114, 301, 331, 401, 536
FaiI YATR 7 cut(s) 62, 112, 171, 299, 329, 399, 534
FalI AAGNNNNNCTT 2 cut(s) 468, 500
FatI CATG 6 cut(s) 60, 110, 297, 327, 397, 532
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 1 cut(s) 188
GluI GCNGC 1 cut(s) 530
HaeIII GGCC 2 cut(s) 131, 167
Hin1II CATG 6 cut(s) 64, 114, 301, 331, 401, 536
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 3 cut(s) 195, 287, 338
HpaI GTTAAC 1 cut(s) 498
HphI GGTGA 5 cut(s) 65, 248, 333, 399, 430
Hpy166II GTNNAC 2 cut(s) 364, 498
Hpy188I TCNGA 7 cut(s) 127, 157, 253, 286, 424, 469, 524
Hpy188III TCNNGA 2 cut(s) 220, 352
Hpy8I GTNNAC 2 cut(s) 364, 498
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 4 cut(s) 44, 368, 415, 450
HpyCH4V TGCA 5 cut(s) 110, 260, 364, 371, 462
HpyF3I CTNAG 1 cut(s) 521
Hsp92II CATG 6 cut(s) 64, 114, 301, 331, 401, 536
KspAI GTTAAC 1 cut(s) 498
Kzo9I GATC 2 cut(s) 88, 238
LmnI GCTCC 1 cut(s) 469
LpnPI CCDG 6 cut(s) 177, 215, 231, 258, 357, 501
Lsp1109I GCAGC 1 cut(s) 541
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 3 cut(s) 38, 300, 405
MalI GATC 2 cut(s) 90, 240
MboI GATC 2 cut(s) 88, 238
MboII GAAGA 5 cut(s) 128, 194, 233, 380, 476
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 3 cut(s) 27, 366, 474
MluCI AATT 2 cut(s) 11, 376
MlyI GAGTC 1 cut(s) 189
MnlI CCTC 4 cut(s) 121, 142, 526, 530
MseI TTAA 2 cut(s) 78, 497
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NdeII GATC 2 cut(s) 88, 238
NlaIII CATG 6 cut(s) 64, 114, 301, 331, 401, 536
NmuCI GTSAC 1 cut(s) 405
NspI RCATGY 2 cut(s) 114, 301
PaeR7I CTCGAG 1 cut(s) 352
PceI AGGCCT 1 cut(s) 131
PfeI GAWTC 2 cut(s) 287, 338
PkrI GCNGC 1 cut(s) 531
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspPI GGNCC 1 cut(s) 165
PsuI RGATCY 1 cut(s) 88
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 78, 497
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 2 cut(s) 88, 238
Sau96I GGNCC 1 cut(s) 165
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 3 cut(s) 27, 366, 474
SetI ASST 3 cut(s) 27, 189, 307
Sfr274I CTCGAG 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 220, 352
SmoI CTYRAG 2 cut(s) 220, 352
Sse9I AATT 2 cut(s) 11, 376
SseBI AGGCCT 1 cut(s) 131
SspMI CTAG 1 cut(s) 188
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 244
TaaI ACNGT 4 cut(s) 44, 368, 415, 450
TaqI TCGA 3 cut(s) 95, 198, 353
TasI AATT 2 cut(s) 11, 376
TfiI GAWTC 2 cut(s) 287, 338
Tru1I TTAA 2 cut(s) 78, 497
Tru9I TTAA 2 cut(s) 78, 497
TscAI CASTG 3 cut(s) 40, 364, 373
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 2 cut(s) 4, 96
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 3 cut(s) 40, 364, 373
VneI GTGCAC 1 cut(s) 362
XapI RAATTY 1 cut(s) 376
XceI RCATGY 2 cut(s) 114, 301
XhoI CTCGAG 1 cut(s) 352
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.