AT3G12600

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006776
Physical Location & Seq
Forward (+)
4004469 .. 4006330
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12600.2

Sequence Viewer

Length: 543 bp
ATGTGTGATTTGGTCGCGCGTACGGGTCGTCTCCAGCAGCGGTACGAGGATGGCTCTCGTCTCGTCGCCGGGTGTATTCCGTTTAGGTATGTAAATTCTGACAAAGATGGTAATTCTGAATCTGGGAAAGTGATTCAAGTGTTGATGATCAGCTCGTCTAGTGGACCTGGACTTTTGTTTCCCAAGGGAGGATGGGAGAATGATGAGACAGTCAGGGAGGCTGCAGCGAGGGAAGCAGTGGAAGAAGCAGGAGTCCGTGGGATTCTTATGGATTTTTTGGGGAATTATGAGTTCAAGAGCAAATCACACCAAGATGAGTTTAGCCCAGAAGGTTTATGTAAAGCGGCAATGTACGCGTTGTATGTGAAAGAAGAGCTAGCGACGTGGCCGGAACATGAGACGAGAACACGTAAATGGCTGACAATTGAAGAAGCGGTAGAGAGTTGCAGGCACCCTTGGATGAAGGACGCATTGGTGGAAGGATTCTGTAAATGGCATAAGGAGAAGATGGTTAAAGGAGAGGAGATAACTGGTGAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

180

Amino Acids

20.39

Weight (kDa)

5.35

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 37 - 146 6.2e-15 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 450
AccII CGCG 3 cut(s) 17, 19, 356
AciI CCGC 3 cut(s) 40, 344, 434
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 1 cut(s) 94
AfaI GTAC 3 cut(s) 22, 44, 353
AfiI CCNNNNNNNGG 1 cut(s) 188
AflIII ACRYGT 2 cut(s) 354, 407
AgsI TTSAA 3 cut(s) 137, 295, 428
AjiI CACGTC 1 cut(s) 384
AjnI CCWGG 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AluBI AGCT 2 cut(s) 153, 376
AluI AGCT 2 cut(s) 153, 376
Alw26I GTCTC 4 cut(s) 35, 65, 200, 392
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 3 cut(s) 37, 221, 224
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 70
AsuNHI GCTAGC 1 cut(s) 376
AvaII GGWCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 49, 208, 236
BccI CCATC 4 cut(s) 44, 101, 186, 502
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 4 cut(s) 35, 65, 200, 392
BfaI CTAG 2 cut(s) 159, 377
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 4 cut(s) 38, 222, 225, 345
BlsI GCNGC 4 cut(s) 39, 223, 226, 346
Bme1390I CCNGG 2 cut(s) 70, 168
Bme18I GGWCC 1 cut(s) 164
BmgBI CACGTC 1 cut(s) 384
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 2 cut(s) 70, 168
BmtI GCTAGC 1 cut(s) 380
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 183, 256, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 535
Bse3DI GCAATG 1 cut(s) 354
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 183, 256, 455
BseGI GGATG 3 cut(s) 55, 197, 465
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 3 cut(s) 49, 208, 236
Bsh1236I CGCG 3 cut(s) 17, 19, 356
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 1 cut(s) 450
BsiSI CCGG 2 cut(s) 69, 389
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 4 cut(s) 35, 65, 200, 392
BsmBI CGTCTC 3 cut(s) 35, 65, 392
BsnI GGCC 1 cut(s) 388
Bsp143I GATC 1 cut(s) 147
BspACI CCGC 3 cut(s) 40, 344, 434
BspANI GGCC 1 cut(s) 388
BspFNI CGCG 3 cut(s) 17, 19, 356
BspLI GGNNCC 1 cut(s) 452
BspMAI CTGCAG 1 cut(s) 226
BspOI GCTAGC 1 cut(s) 380
BspQI GCTCTTC 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 354
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 3 cut(s) 183, 256, 455
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 183, 455
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 378, 449
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 3 cut(s) 55, 197, 465
BstFNI CGCG 3 cut(s) 17, 19, 356
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 4 cut(s) 35, 65, 200, 392
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 233, 353
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 68, 166
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 3 cut(s) 17, 19, 356
BstV1I GCAGC 3 cut(s) 49, 208, 236
BsuRI GGCC 1 cut(s) 388
BtgI CCRYGG 1 cut(s) 256
BtrI CACGTC 1 cut(s) 384
BtsCI GGATG 3 cut(s) 55, 197, 465
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 378, 449
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 476
Csp6I GTAC 3 cut(s) 21, 43, 352
CviAII CATG 1 cut(s) 395
CviJI RGCY 7 cut(s) 54, 153, 221, 324, 376, 388, 418
CviKI_1 RGCY 7 cut(s) 54, 153, 221, 324, 376, 388, 418
CviQI GTAC 3 cut(s) 21, 43, 352
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Eco130I CCWWGG 2 cut(s) 183, 455
Eco47I GGWCC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 2 cut(s) 183, 455
ErhI CCWWGG 2 cut(s) 183, 455
Esp3I CGTCTC 3 cut(s) 35, 65, 392
FaeI CATG 1 cut(s) 398
FaiI YATR 7 cut(s) 90, 269, 288, 337, 363, 396, 498
FatI CATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 38, 222, 225, 345
FokI GGATG 3 cut(s) 62, 204, 472
Fsp4HI GCNGC 4 cut(s) 38, 222, 225, 345
FspBI CTAG 2 cut(s) 159, 377
GlaI GCGC 1 cut(s) 18
GluI GCNGC 4 cut(s) 38, 222, 225, 345
GsuI CTGGAG 1 cut(s) 17
HaeIII GGCC 1 cut(s) 388
HapII CCGG 2 cut(s) 69, 389
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 19
Hin1II CATG 1 cut(s) 398
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 5 cut(s) 119, 133, 252, 262, 483
HpaII CCGG 2 cut(s) 69, 389
Hpy166II GTNNAC 2 cut(s) 164, 536
Hpy188I TCNGA 2 cut(s) 100, 118
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 2 cut(s) 164, 536
Hpy99I CGWCG 2 cut(s) 68, 385
HpyAV CCTTC 3 cut(s) 323, 457, 473
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 2 cut(s) 383, 409
HpyCH4V TGCA 2 cut(s) 224, 447
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 353
HpySE526I ACGT 2 cut(s) 383, 409
Hsp92II CATG 1 cut(s) 398
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 366
Lsp1109I GCAGC 3 cut(s) 49, 208, 236
MaeI CTAG 2 cut(s) 159, 377
MaeII ACGT 2 cut(s) 383, 409
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 4 cut(s) 254, 383, 440, 517
MfeI CAATTG 1 cut(s) 423
MluCI AATT 4 cut(s) 94, 112, 283, 423
MluI ACGCGT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 261
MnlI CCTC 5 cut(s) 40, 182, 211, 222, 514
MseI TTAA 1 cut(s) 513
MslI CAYNNNNRTG 2 cut(s) 312, 412
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 2 cut(s) 69, 389
MspR9I CCNGG 2 cut(s) 70, 168
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 3 cut(s) 17, 19, 356
MwoI GCNNNNNNNGC 2 cut(s) 233, 353
NciI CCSGG 1 cut(s) 70
NdeII GATC 1 cut(s) 147
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 4 cut(s) 119, 133, 262, 483
Pfl23II CGTACG 1 cut(s) 20
PkrI GCNGC 4 cut(s) 39, 223, 226, 346
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspLI CGTACG 1 cut(s) 20
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 1 cut(s) 164
PstI CTGCAG 1 cut(s) 226
RsaI GTAC 3 cut(s) 22, 44, 353
RsaNI GTAC 3 cut(s) 21, 43, 352
RseI CAYNNNNRTG 2 cut(s) 312, 412
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 38, 222, 225, 345
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 2 cut(s) 70, 168
SetI ASST 7 cut(s) 89, 155, 169, 334, 378, 386, 412
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 2 cut(s) 312, 412
Sse9I AATT 4 cut(s) 94, 112, 283, 423
SsiI CCGC 3 cut(s) 40, 344, 434
SspMI CTAG 2 cut(s) 159, 377
StyD4I CCNGG 2 cut(s) 68, 166
StyI CCWWGG 2 cut(s) 183, 455
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 2 cut(s) 386, 412
TasI AATT 4 cut(s) 94, 112, 283, 423
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 4 cut(s) 119, 133, 262, 483
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 1 cut(s) 243
TseI GCWGC 3 cut(s) 37, 221, 224
TspDTI ATGAA 1 cut(s) 476
TspGWI ACGGA 2 cut(s) 69, 245
TspRI CASTG 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 94
XspI CTAG 2 cut(s) 159, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.