MD07G1048400.v1.1

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006776
Physical Location & Seq
Reverse (-)
4143090 .. 4147447
4358 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1048400.v1.1.491

Sequence Viewer

Length: 549 bp
ATGTGTGATATAGTGGCCCGTACGGGTCGGCATCAGCAACGCTACGAGGCCGGATGTCGCCTTGTTGCCGGGTGTATTCCGTTTAGATACAAAAGCTCTGATGAGACTGATGATGCCAATTCTGAGAAGATCATTGAGGTGCTTATGATTAACTCACCTAGTGGACCTGGTCTTTTGTTTCCAAAGGGAGGCTGGGAGAATGATGAAACTGTTGAGCAGGCTGCCGTAAGGGAAGCTGTTGAAGAAGCTGGAGTTCGGGGAGAGCTAATGGATTTCCTGGGGTATTATTATTTTAAGAGCAAAACACACCAGGACGAGTTCAGTCCGGAAGGTGTATGTAGAGCTTCTATGTTTGCTTTGTTTGTGAAAGAGGAGCTTGAGTCATGGCCTGAACAGAGCACCCGAGACAGAACATGGCTGACTGTAGCAGAGGCTATCGTGAGTTGCCGGCATGAATGGATGAGGGATGCCCTGGAGGGAGGCTTCTCAAAGTGGCATGCAGAAAAGAAGATAGGTACAGGGGAGGAGGATGTAACATTGACTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

183

Amino Acids

20.61

Weight (kDa)

4.83

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 150 3.7e-14 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 2 cut(s) 22, 517
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 242
AjnI CCWGG 4 cut(s) 166, 276, 309, 471
AluBI AGCT 6 cut(s) 96, 236, 248, 265, 344, 376
AluI AGCT 6 cut(s) 96, 236, 248, 265, 344, 376
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 98, 399
Ama87I CYCGRG 1 cut(s) 402
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 3 cut(s) 15, 48, 386
ApeKI GCWGC 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 16, 164
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 208
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 4 cut(s) 168, 278, 311, 473
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 98, 399
BfaI CTAG 2 cut(s) 159, 547
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 5 cut(s) 70, 168, 278, 311, 473
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 402
BmgT120I GGNCC 2 cut(s) 16, 164
BmrFI CCNGG 5 cut(s) 70, 168, 278, 311, 473
BmsI GCATC 3 cut(s) 40, 103, 457
BpmI CTGGAG 2 cut(s) 270, 494
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 70
BsaJI CCNNGG 2 cut(s) 277, 471
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 4 cut(s) 168, 278, 311, 473
BseDI CCNNGG 2 cut(s) 277, 471
BseGI GGATG 4 cut(s) 59, 465, 472, 535
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 2 cut(s) 386, 539
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 3 cut(s) 17, 50, 388
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 4 cut(s) 51, 69, 326, 448
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 2 cut(s) 98, 399
BsnI GGCC 3 cut(s) 17, 50, 388
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 401
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 1 cut(s) 129
BspANI GGCC 3 cut(s) 17, 50, 388
BspCNI CTCAG 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 447
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 2 cut(s) 277, 471
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 4 cut(s) 168, 278, 311, 473
Bst4CI ACNGT 2 cut(s) 211, 424
BstC8I GCNNGC 3 cut(s) 219, 449, 498
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 4 cut(s) 59, 465, 472, 535
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 2 cut(s) 98, 399
BstMBI GATC 1 cut(s) 129
BstNI CCWGG 4 cut(s) 168, 278, 311, 473
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 5 cut(s) 68, 166, 276, 309, 471
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 208
BsuRI GGCC 3 cut(s) 17, 50, 388
BtsCI GGATG 4 cut(s) 59, 465, 472, 535
Cac8I GCNNGC 3 cut(s) 219, 449, 498
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 2 cut(s) 16, 164
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 21, 516
CviAII CATG 4 cut(s) 384, 414, 452, 497
CviQI GTAC 2 cut(s) 21, 516
DdeI CTNAG 1 cut(s) 123
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
DraIII CACNNNGTG 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 402
EcoRII CCWGG 4 cut(s) 166, 276, 309, 471
FaeI CATG 4 cut(s) 387, 417, 455, 500
FaiI YATR 8 cut(s) 11, 146, 337, 350, 385, 415, 453, 498
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 4 cut(s) 383, 413, 451, 496
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 4 cut(s) 66, 472, 479, 542
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 2 cut(s) 159, 547
GluI GCNGC 1 cut(s) 222
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 2 cut(s) 270, 494
HaeIII GGCC 3 cut(s) 17, 50, 388
HapII CCGG 4 cut(s) 51, 69, 326, 448
Hin1II CATG 4 cut(s) 387, 417, 455, 500
HinfI GANTC 1 cut(s) 380
HpaII CCGG 4 cut(s) 51, 69, 326, 448
HphI GGTGA 1 cut(s) 147
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 2 cut(s) 100, 124
Hpy188III TCNNGA 2 cut(s) 326, 439
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 2 cut(s) 211, 424
HpyCH4V TGCA 1 cut(s) 500
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 4 cut(s) 387, 417, 455, 500
Kpn2I TCCGGA 1 cut(s) 325
KroI GCCGGC 1 cut(s) 447
KroNI GCCGGC 1 cut(s) 449
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 3 cut(s) 40, 103, 457
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 2 cut(s) 159, 547
MaeIII GTNAC 1 cut(s) 532
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 3 cut(s) 139, 254, 520
MhlI GDGCHC 1 cut(s) 401
MluCI AATT 1 cut(s) 118
MlyI GAGTC 1 cut(s) 389
MroI TCCGGA 1 cut(s) 325
MroNI GCCGGC 1 cut(s) 447
MseI TTAA 2 cut(s) 150, 294
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 4 cut(s) 51, 69, 326, 448
MspR9I CCNGG 5 cut(s) 70, 168, 278, 311, 473
MvaI CCWGG 4 cut(s) 168, 278, 311, 473
NaeI GCCGGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 70
NdeII GATC 1 cut(s) 129
NgoMIV GCCGGC 1 cut(s) 447
NlaIII CATG 4 cut(s) 387, 417, 455, 500
NspI RCATGY 1 cut(s) 500
PaeI GCATGC 1 cut(s) 500
PdiI GCCGGC 1 cut(s) 449
Pfl23II CGTACG 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 4 cut(s) 166, 276, 309, 471
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 4 cut(s) 166, 276, 309, 471
PspLI CGTACG 1 cut(s) 20
PspPI GGNCC 2 cut(s) 16, 164
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 2 cut(s) 22, 517
RsaNI GTAC 2 cut(s) 21, 516
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 2 cut(s) 150, 294
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 2 cut(s) 16, 164
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 5 cut(s) 70, 168, 278, 311, 473
SduI GDGCHC 1 cut(s) 401
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 3 cut(s) 40, 103, 457
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 1 cut(s) 500
Sse9I AATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 159, 547
StyD4I CCNGG 5 cut(s) 68, 166, 276, 309, 471
TaaI ACNGT 2 cut(s) 211, 424
TasI AATT 1 cut(s) 118
Tru1I TTAA 2 cut(s) 150, 294
Tru9I TTAA 2 cut(s) 150, 294
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 219, 468
TspGWI ACGGA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 164
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XspI CTAG 2 cut(s) 159, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.