MD02G1282400.v1.1

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006776
Physical Location & Seq
Reverse (-)
33841916 .. 33847278
5363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1282400.v1.1.491

Sequence Viewer

Length: 525 bp
ATGTGTGATATAGTGGCCCGTACGGGTCGGCATCAGCAACGCTACGAGGCCGGTTGTCGCCTTGTTGCCGGGTGTATTCCATTTAGATACAAAAATTCTGATGAGACCGATGAGGCGAATTCTGAGAAGATCATTGAGGTGCTTATGATTAACTCACCTAGTGGACCAGGTCTTTTGTTTCCAAAGGGAGGCTGGGAGAATGATGAAACTGTTGAGGAGGCAGCCGCAAGGGAAGCTGTTGAAGAAGCTGGAGTTCGAGGAGAGCTAATGGGTTTCCTGGGGTATTATTATTTTAAGAGCAAGACACACCAGGATGAGTTCAGTCCGGAAGGTGTATGTAAAGCTGCTATGTTTGCTTTGTTTGTCAAGGAGGAACTTGAGTCATGGCCCGAACAGAGCACCCGAAACAGAACATGGCTGACTGTAGCAGAGGCTATCGTGAGTTGCCGGCATGCATGGATGAGGGATGCCCTGGTGGAAGGCTTCTCAAAGTGGCATGCAGAAAAGATGATAGGTACGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

175

Amino Acids

19.62

Weight (kDa)

5.11

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 146 1.7e-13 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 1 cut(s) 225
AcsI RAATTY 2 cut(s) 94, 118
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 2 cut(s) 22, 517
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 242
AjnI CCWGG 4 cut(s) 166, 276, 309, 471
AluBI AGCT 4 cut(s) 236, 248, 265, 344
AluI AGCT 4 cut(s) 236, 248, 265, 344
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 3 cut(s) 15, 48, 386
ApeKI GCWGC 2 cut(s) 221, 344
ApoI RAATTY 2 cut(s) 94, 118
AspS9I GGNCC 3 cut(s) 16, 164, 387
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 147
AvaII GGWCC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 233, 331
BciT130I CCWGG 4 cut(s) 168, 278, 311, 473
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 3 cut(s) 222, 225, 345
BlsI GCNGC 3 cut(s) 223, 226, 346
Bme1390I CCNGG 5 cut(s) 70, 168, 278, 311, 473
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 3 cut(s) 16, 164, 387
BmrFI CCNGG 5 cut(s) 70, 168, 278, 311, 473
BmsI GCATC 2 cut(s) 40, 457
BpmI CTGGAG 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 277, 471
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 2 cut(s) 50, 447
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 4 cut(s) 168, 278, 311, 473
BseDI CCNNGG 2 cut(s) 277, 471
BseGI GGATG 3 cut(s) 319, 465, 472
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 2 cut(s) 230, 273
BseXI GCAGC 2 cut(s) 233, 331
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 3 cut(s) 17, 50, 388
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 4 cut(s) 51, 69, 326, 448
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 3 cut(s) 17, 50, 388
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 401
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 1 cut(s) 129
BspACI CCGC 1 cut(s) 225
BspANI GGCC 3 cut(s) 17, 50, 388
BspCNI CTCAG 1 cut(s) 115
BspEI TCCGGA 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 98
BsrFI RCCGGY 2 cut(s) 50, 447
BssAI RCCGGY 2 cut(s) 50, 447
BssECI CCNNGG 2 cut(s) 277, 471
BssMI GATC 1 cut(s) 129
Bst2UI CCWGG 4 cut(s) 168, 278, 311, 473
Bst4CI ACNGT 2 cut(s) 211, 424
BstC8I GCNNGC 3 cut(s) 449, 453, 498
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 319, 465, 472
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 233, 353
BstNI CCWGG 4 cut(s) 168, 278, 311, 473
BstNSI RCATGY 2 cut(s) 455, 500
BstSCI CCNGG 5 cut(s) 68, 166, 276, 309, 471
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 233, 331
BsuRI GGCC 3 cut(s) 17, 50, 388
BtsCI GGATG 3 cut(s) 319, 465, 472
Cac8I GCNNGC 3 cut(s) 449, 453, 498
Cfr10I RCCGGY 2 cut(s) 50, 447
Cfr13I GGNCC 3 cut(s) 16, 164, 387
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 21, 516
CviAII CATG 5 cut(s) 384, 414, 452, 456, 497
CviQI GTAC 2 cut(s) 21, 516
DdeI CTNAG 1 cut(s) 123
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
DraIII CACNNNGTG 1 cut(s) 161
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 4 cut(s) 166, 276, 309, 471
EcoT22I ATGCAT 1 cut(s) 457
FaeI CATG 5 cut(s) 387, 417, 455, 459, 500
FaiI YATR 9 cut(s) 11, 146, 337, 350, 385, 415, 453, 457, 498
FatI CATG 5 cut(s) 383, 413, 451, 455, 496
Fnu4HI GCNGC 3 cut(s) 222, 225, 345
FokI GGATG 3 cut(s) 326, 472, 479
Fsp4HI GCNGC 3 cut(s) 222, 225, 345
FspBI CTAG 1 cut(s) 159
GluI GCNGC 3 cut(s) 222, 225, 345
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 3 cut(s) 17, 50, 388
HapII CCGG 4 cut(s) 51, 69, 326, 448
Hin1II CATG 5 cut(s) 387, 417, 455, 459, 500
HinfI GANTC 1 cut(s) 380
HpaII CCGG 4 cut(s) 51, 69, 326, 448
HphI GGTGA 1 cut(s) 147
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 2 cut(s) 100, 124
Hpy188III TCNNGA 2 cut(s) 326, 439
Hpy8I GTNNAC 1 cut(s) 164
HpyAV CCTTC 2 cut(s) 323, 473
HpyCH4III ACNGT 2 cut(s) 211, 424
HpyCH4V TGCA 2 cut(s) 455, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 353
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 5 cut(s) 387, 417, 455, 459, 500
Kpn2I TCCGGA 1 cut(s) 325
KroI GCCGGC 1 cut(s) 447
KroNI GCCGGC 1 cut(s) 449
Kzo9I GATC 1 cut(s) 129
Lsp1109I GCAGC 2 cut(s) 233, 331
LweI GCATC 2 cut(s) 40, 457
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 1 cut(s) 159
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 2 cut(s) 139, 254
MhlI GDGCHC 1 cut(s) 401
MluCI AATT 2 cut(s) 94, 118
MlyI GAGTC 1 cut(s) 389
Mph1103I ATGCAT 1 cut(s) 457
MroI TCCGGA 1 cut(s) 325
MroNI GCCGGC 1 cut(s) 447
MseI TTAA 2 cut(s) 150, 294
MslI CAYNNNNRTG 2 cut(s) 137, 312
MspI CCGG 4 cut(s) 51, 69, 326, 448
MspR9I CCNGG 5 cut(s) 70, 168, 278, 311, 473
MvaI CCWGG 4 cut(s) 168, 278, 311, 473
MwoI GCNNNNNNNGC 2 cut(s) 233, 353
NaeI GCCGGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 70
NdeII GATC 1 cut(s) 129
NgoMIV GCCGGC 1 cut(s) 447
NlaIII CATG 5 cut(s) 387, 417, 455, 459, 500
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 2 cut(s) 455, 500
PaeI GCATGC 2 cut(s) 455, 500
PdiI GCCGGC 1 cut(s) 449
Pfl23II CGTACG 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 3 cut(s) 223, 226, 346
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Psp6I CCWGG 4 cut(s) 166, 276, 309, 471
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 4 cut(s) 166, 276, 309, 471
PspLI CGTACG 1 cut(s) 20
PspPI GGNCC 3 cut(s) 16, 164, 387
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 2 cut(s) 22, 517
RsaNI GTAC 2 cut(s) 21, 516
RseI CAYNNNNRTG 2 cut(s) 137, 312
SaqAI TTAA 2 cut(s) 150, 294
SatI GCNGC 3 cut(s) 222, 225, 345
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 3 cut(s) 16, 164, 387
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 5 cut(s) 70, 168, 278, 311, 473
SduI GDGCHC 1 cut(s) 401
SetI ASST 9 cut(s) 141, 160, 172, 238, 250, 267, 334, 346, 517
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 2 cut(s) 40, 457
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 2 cut(s) 137, 312
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 2 cut(s) 455, 500
Sse9I AATT 2 cut(s) 94, 118
SsiI CCGC 1 cut(s) 225
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 5 cut(s) 68, 166, 276, 309, 471
TaaI ACNGT 2 cut(s) 211, 424
TaqI TCGA 1 cut(s) 256
TaqII GACCGA 1 cut(s) 122
TasI AATT 2 cut(s) 94, 118
TauI GCSGC 1 cut(s) 227
Tru1I TTAA 2 cut(s) 150, 294
Tru9I TTAA 2 cut(s) 150, 294
TseI GCWGC 2 cut(s) 221, 344
TspDTI ATGAA 1 cut(s) 219
Tth111I GACNNNGTC 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 94, 118
XceI RCATGY 2 cut(s) 455, 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XspI CTAG 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.