Rh1BG058200

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006776
Physical Location & Seq
Forward (+)
8556004 .. 8558810
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG058200.1

Sequence Viewer

Length: 486 bp
ATGAAGAAACTAACTCAGGTGATCTTTAACAGGTGTATTCCATTCAGGTACAGAAATTCTGATGAGAGTGGCGATGCTGATTCTGAGAAGATTGTTGAGGTCCTTATGATTAACTCATCCAGTGGACCAGGTCTTTTATTTCCTAAGGGAGGCTGGGAGAATGATGAAACTGTGCAGGAAGCTGCTGTAAGAGAAGCTATTGAAGAAGCTGGGGTTCGAGGAGAACTAATGGATTTAGTGGGGTATTACCAATTTAAGAGTAAAACTCACCAGGATGAGTTAAACCCAGAAGGTATATGTAGAGCTGCTATGTTTGCATTGTTTGTCAAGGAGGAGCTTGAGTCATGGCCAGAACAGAGCACTCGAAACAGAACTTGGCTGACTGTTCAAGAGGCTACTGGTAGATGCCGGCATGCATGGATGAGTGAAGCTCTGGTGGAGGGCTTCTCAAAGTGGCATGCAGAAAAGATGATAGGTGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

161

Amino Acids

18.26

Weight (kDa)

5.08

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 34 - 132 2.1e-13 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 347
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 2 cut(s) 203, 389
AjnI CCWGG 2 cut(s) 127, 270
AjuI GAANNNNNNNTTGG 2 cut(s) 358, 390
AluBI AGCT 6 cut(s) 182, 197, 209, 305, 337, 431
AluI AGCT 6 cut(s) 182, 197, 209, 305, 337, 431
Alw21I GWGCWC 1 cut(s) 362
AoxI GGCC 1 cut(s) 347
ApeKI GCWGC 2 cut(s) 182, 305
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 100, 125
AsuHPI GGTGA 2 cut(s) 31, 260
AvaII GGWCC 2 cut(s) 100, 125
AxyI CCTNAGG 1 cut(s) 144
BalI TGGCCA 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 169, 292
BciT130I CCWGG 2 cut(s) 129, 272
BisI GCNGC 2 cut(s) 183, 306
BlsI GCNGC 2 cut(s) 184, 307
Bme1390I CCNGG 2 cut(s) 129, 272
Bme18I GGWCC 2 cut(s) 100, 125
BmgT120I GGNCC 2 cut(s) 100, 125
BmrFI CCNGG 2 cut(s) 129, 272
BmsI GCATC 2 cut(s) 64, 395
BplI GAGNNNNNCTC 6 cut(s) 250, 282, 415, 447, 431, 463
BpuEI CTTGAG 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 120, 403
Bse21I CCTNAGG 1 cut(s) 144
BseBI CCWGG 2 cut(s) 129, 272
BseGI GGATG 3 cut(s) 116, 280, 426
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 2 cut(s) 29, 75
BseNI ACTGG 2 cut(s) 120, 403
BseRI GAGGAG 2 cut(s) 234, 347
BseXI GCAGC 2 cut(s) 169, 292
BseYI CCCAGC 2 cut(s) 153, 209
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 362
BsiSI CCGG 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 149
BsnI GGCC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 1 cut(s) 21
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 2 cut(s) 28, 76
BsrFI RCCGGY 1 cut(s) 408
BsrI ACTGG 2 cut(s) 120, 403
BssAI RCCGGY 1 cut(s) 408
BssMI GATC 1 cut(s) 21
Bst2UI CCWGG 2 cut(s) 129, 272
Bst4CI ACNGT 2 cut(s) 172, 385
BstC8I GCNNGC 3 cut(s) 410, 414, 459
BstDEI CTNAG 3 cut(s) 15, 84, 144
BstENI CCTNNNNNAGG 1 cut(s) 147
BstF5I GGATG 3 cut(s) 116, 280, 426
BstKTI GATC 1 cut(s) 24
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstNI CCWGG 2 cut(s) 129, 272
BstNSI RCATGY 2 cut(s) 416, 461
BstSCI CCNGG 2 cut(s) 127, 270
BstV1I GCAGC 2 cut(s) 169, 292
Bsu36I CCTNAGG 1 cut(s) 144
BsuRI GGCC 1 cut(s) 349
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 116, 280, 426
BtsIMutI CAGTG 1 cut(s) 127
Cac8I GCNNGC 3 cut(s) 410, 414, 459
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 100, 125
CsiI ACCWGGT 1 cut(s) 127
Csp6I GTAC 1 cut(s) 49
CviAII CATG 4 cut(s) 345, 413, 417, 458
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 15, 84, 144
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
EaeI YGGCCR 1 cut(s) 347
Eco47I GGWCC 2 cut(s) 100, 125
Eco81I CCTNAGG 1 cut(s) 144
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 127, 270
EcoT22I ATGCAT 1 cut(s) 418
FaeI CATG 4 cut(s) 348, 416, 420, 461
FaiI YATR 8 cut(s) 107, 296, 298, 311, 346, 414, 418, 459
FatI CATG 4 cut(s) 344, 412, 416, 457
Fnu4HI GCNGC 2 cut(s) 183, 306
FokI GGATG 3 cut(s) 103, 287, 433
Fsp4HI GCNGC 2 cut(s) 183, 306
GluI GCNGC 2 cut(s) 183, 306
GsaI CCCAGC 2 cut(s) 157, 213
HaeIII GGCC 1 cut(s) 349
HapII CCGG 1 cut(s) 409
Hin1II CATG 4 cut(s) 348, 416, 420, 461
HinfI GANTC 2 cut(s) 80, 341
HpaII CCGG 1 cut(s) 409
HphI GGTGA 2 cut(s) 31, 260
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 2 cut(s) 61, 85
Hpy188III TCNNGA 1 cut(s) 389
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 2 cut(s) 172, 385
HpyCH4V TGCA 5 cut(s) 175, 317, 416, 461, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 3 cut(s) 15, 84, 144
Hsp92II CATG 4 cut(s) 348, 416, 420, 461
KroI GCCGGC 1 cut(s) 408
KroNI GCCGGC 1 cut(s) 410
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 2 cut(s) 169, 292
LweI GCATC 2 cut(s) 64, 395
MabI ACCWGGT 1 cut(s) 127
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 3 cut(s) 16, 100, 215
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 1 cut(s) 349
MluCI AATT 2 cut(s) 55, 251
MluNI TGGCCA 1 cut(s) 349
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 6 cut(s) 91, 143, 212, 325, 385, 433
Mox20I TGGCCA 1 cut(s) 349
Mph1103I ATGCAT 1 cut(s) 418
MroNI GCCGGC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 349
MseI TTAA 4 cut(s) 27, 111, 255, 281
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 349
MspI CCGG 1 cut(s) 409
MspR9I CCNGG 2 cut(s) 129, 272
MvaI CCWGG 2 cut(s) 129, 272
MwoI GCNNNNNNNGC 1 cut(s) 314
NaeI GCCGGC 1 cut(s) 410
NdeII GATC 1 cut(s) 21
NgoMIV GCCGGC 1 cut(s) 408
NlaIII CATG 4 cut(s) 348, 416, 420, 461
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 2 cut(s) 416, 461
PaeI GCATGC 2 cut(s) 416, 461
PdiI GCCGGC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 80
PflFI GACNNNGTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 184, 307
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 127, 270
PspFI CCCAGC 2 cut(s) 153, 209
PspGI CCWGG 2 cut(s) 127, 270
PspPI GGNCC 2 cut(s) 100, 125
PspPPI RGGWCCY 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 129
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 4 cut(s) 27, 111, 255, 281
SatI GCNGC 2 cut(s) 183, 306
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 2 cut(s) 100, 125
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 129, 272
SduI GDGCHC 1 cut(s) 362
SexAI ACCWGGT 1 cut(s) 127
SfaNI GCATC 2 cut(s) 64, 395
SinI GGWCC 2 cut(s) 100, 125
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SphI GCATGC 2 cut(s) 416, 461
Sse9I AATT 2 cut(s) 55, 251
StyD4I CCNGG 2 cut(s) 127, 270
TaaI ACNGT 2 cut(s) 172, 385
TaqI TCGA 2 cut(s) 217, 364
TasI AATT 2 cut(s) 55, 251
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 4 cut(s) 27, 111, 255, 281
Tru9I TTAA 4 cut(s) 27, 111, 255, 281
TscAI CASTG 1 cut(s) 127
TseI GCWGC 2 cut(s) 182, 305
TspDTI ATGAA 2 cut(s) 17, 180
TspRI CASTG 1 cut(s) 127
Tth111I GACNNNGTC 1 cut(s) 129
VpaK11BI GGWCC 2 cut(s) 100, 125
XagI CCTNNNNNAGG 1 cut(s) 147
XapI RAATTY 1 cut(s) 55
XceI RCATGY 2 cut(s) 416, 461
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.