MD14G1088400.v1.1

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006776
Physical Location & Seq
Forward (+)
11063228 .. 11068969
5742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1088400.v1.1.491

Sequence Viewer

Length: 570 bp
ATGTCCGACTTGGTGGCTCGCACCGGCCGCCACCAGCAGCGCTACGAGGCCGGCCACCGCCTTGTCGCCGGGTGTATTCCTTTCAAATATAGAAATACTGGAGAAAATAGTGATGGCACATATGAGAAGGTTGTTGAGGTACTAATGATCAGCACAACTAGTGGACCTGGGCTTGTGTTTCCAAAGGGTGGATGGGAAAATGATGAAACCGTTCAGGAAGCAGCAATACGTGAAGCTGTGGAAGAGGCTGGAGTACAAGGGGATATAATGGATTGTTTGGGGCACTATTTTTTTAAGAGCAAGACCCTGCAAGATGAACTTAGTCCCGAGGGATTGTGCAAAGCTGCCATGTTTCCTTTGCTTGTGAAGGAAGAGCTCGAGTTTTGGCCAGAACAAAACAATCGTCGGCGAAGTTGGCATTCCATCCCTGAAGCTATAGAACTTTGCCGGCATCAATGGATGAAAGCGGCCCTTGAGGAAGGTTTCTCAAGGTGGTTTGCGGAACAGATGATAAGCACTCAAAAAGAGAACCACTTCATTTCACCTGATTCATCTGCAGACCAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

190

Amino Acids

21.56

Weight (kDa)

5.05

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 149 5.1e-12 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 188
AciI CCGC 4 cut(s) 28, 58, 467, 500
AcoI YGGCCR 3 cut(s) 25, 52, 386
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 2 cut(s) 141, 255
AfeI AGCGCT 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 85
AhlI ACTAGT 1 cut(s) 158
AjnI CCWGG 2 cut(s) 166, 561
AluBI AGCT 4 cut(s) 236, 344, 376, 434
AluI AGCT 4 cut(s) 236, 344, 376, 434
Alw21I GWGCWC 1 cut(s) 378
Ama87I CYCGRG 2 cut(s) 326, 377
Aor51HI AGCGCT 1 cut(s) 41
AoxI GGCC 5 cut(s) 25, 48, 52, 386, 468
ApeKI GCWGC 3 cut(s) 37, 221, 344
ArsI GACNNNNNNTTYG 2 cut(s) 388, 420
Asp700I GAANNNNTTC 2 cut(s) 210, 533
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 164, 469
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 534
AvaI CYCGRG 2 cut(s) 326, 377
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 285
BalI TGGCCA 1 cut(s) 388
BanII GRGCYC 1 cut(s) 378
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 49, 233, 331
BccI CCATC 3 cut(s) 107, 186, 431
BciT130I CCWGG 2 cut(s) 168, 563
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 70
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 2 cut(s) 435, 555
BfoI RGCGCY 1 cut(s) 43
BisI GCNGC 5 cut(s) 28, 38, 222, 345, 468
BlsI GCNGC 5 cut(s) 29, 39, 223, 346, 469
Bme1390I CCNGG 3 cut(s) 70, 168, 563
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 2 cut(s) 326, 377
BmgT120I GGNCC 2 cut(s) 164, 469
BmrFI CCNGG 3 cut(s) 70, 168, 563
BmsI GCATC 1 cut(s) 460
BpmI CTGGAG 2 cut(s) 120, 270
BpuEI CTTGAG 2 cut(s) 472, 494
BpuMI CCSGG 1 cut(s) 70
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 167, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 3 cut(s) 23, 50, 447
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 2 cut(s) 168, 563
BseDI CCNNGG 2 cut(s) 167, 327
BseGI GGATG 3 cut(s) 197, 423, 465
BseLI CCNNNNNNNGG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 285
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 49, 233, 331
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 5 cut(s) 27, 50, 54, 388, 470
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 2 cut(s) 326, 377
BsiSI CCGG 4 cut(s) 24, 51, 69, 448
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 5 cut(s) 27, 50, 54, 388, 470
BsoBI CYCGRG 2 cut(s) 326, 377
Bsp1286I GDGCHC 2 cut(s) 285, 378
Bsp143I GATC 1 cut(s) 147
BspACI CCGC 4 cut(s) 28, 58, 467, 500
BspANI GGCC 5 cut(s) 27, 50, 54, 388, 470
BspMAI CTGCAG 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 366
BsrFI RCCGGY 3 cut(s) 23, 50, 447
BsrI ACTGG 1 cut(s) 103
BssAI RCCGGY 3 cut(s) 23, 50, 447
BssECI CCNNGG 2 cut(s) 167, 327
BssMI GATC 1 cut(s) 147
Bst2UI CCWGG 2 cut(s) 168, 563
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 2 cut(s) 237, 366
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 3 cut(s) 19, 52, 449
BstDEI CTNAG 1 cut(s) 320
BstF5I GGATG 3 cut(s) 197, 423, 465
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 1 cut(s) 150
BstMBI GATC 1 cut(s) 147
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 2 cut(s) 27, 415
BstNI CCWGG 2 cut(s) 168, 563
BstSCI CCNGG 3 cut(s) 68, 166, 561
BstSFI CTRYAG 2 cut(s) 435, 555
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 49, 233, 331
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 5 cut(s) 27, 50, 54, 388, 470
BtsCI GGATG 3 cut(s) 197, 423, 465
Cac8I GCNNGC 3 cut(s) 19, 52, 449
CfoI GCGC 1 cut(s) 42
Cfr10I RCCGGY 3 cut(s) 23, 50, 447
Cfr13I GGNCC 2 cut(s) 164, 469
Csp6I GTAC 2 cut(s) 140, 254
CviAII CATG 1 cut(s) 349
CviQI GTAC 2 cut(s) 140, 254
DdeI CTNAG 1 cut(s) 320
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
EaeI YGGCCR 3 cut(s) 25, 52, 386
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 2 cut(s) 237, 366
EarI CTCTTC 2 cut(s) 237, 366
Ecl136II GAGCTC 1 cut(s) 376
EclXI CGGCCG 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 164
Eco47III AGCGCT 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 2 cut(s) 326, 377
EcoICRI GAGCTC 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 166, 561
EcoT38I GRGCYC 1 cut(s) 378
FaeI CATG 1 cut(s) 352
FaiI YATR 6 cut(s) 90, 121, 123, 266, 350, 437
FalI AAGNNNNNCTT 4 cut(s) 303, 335, 456, 488
FaqI GGGAC 1 cut(s) 309
FatI CATG 1 cut(s) 348
FauNDI CATATG 1 cut(s) 121
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 28, 38, 222, 345, 468
FokI GGATG 3 cut(s) 204, 410, 472
FriOI GRGCYC 1 cut(s) 378
FseI GGCCGGCC 1 cut(s) 54
Fsp4HI GCNGC 5 cut(s) 28, 38, 222, 345, 468
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 41
GluI GCNGC 5 cut(s) 28, 38, 222, 345, 468
GsuI CTGGAG 2 cut(s) 120, 270
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 5 cut(s) 27, 50, 54, 388, 470
HapII CCGG 4 cut(s) 24, 51, 69, 448
HhaI GCGC 1 cut(s) 42
Hin1II CATG 1 cut(s) 352
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 1 cut(s) 548
HpaII CCGG 4 cut(s) 24, 51, 69, 448
HphI GGTGA 1 cut(s) 534
Hpy166II GTNNAC 1 cut(s) 164
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 2 cut(s) 215, 326
Hpy8I GTNNAC 1 cut(s) 164
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 3 cut(s) 121, 361, 473
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 3 cut(s) 310, 339, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 415
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 1 cut(s) 352
HspAI GCGC 1 cut(s) 40
KroI GCCGGC 2 cut(s) 50, 447
KroNI GCCGGC 2 cut(s) 52, 449
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 366
Lsp1109I GCAGC 3 cut(s) 49, 233, 331
LweI GCATC 1 cut(s) 460
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 229
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 2 cut(s) 254, 383
MhlI GDGCHC 2 cut(s) 285, 378
MlsI TGGCCA 1 cut(s) 388
MluNI TGGCCA 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 5 cut(s) 40, 130, 238, 322, 469
Mox20I TGGCCA 1 cut(s) 388
MroNI GCCGGC 2 cut(s) 50, 447
MroXI GAANNNNTTC 2 cut(s) 210, 533
MscI TGGCCA 1 cut(s) 388
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 388
MspI CCGG 4 cut(s) 24, 51, 69, 448
MspR9I CCNGG 3 cut(s) 70, 168, 563
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 2 cut(s) 168, 563
MwoI GCNNNNNNNGC 2 cut(s) 27, 415
NaeI GCCGGC 2 cut(s) 52, 449
NciI CCSGG 1 cut(s) 70
NdeI CATATG 1 cut(s) 121
NdeII GATC 1 cut(s) 147
NgoMIV GCCGGC 2 cut(s) 50, 447
NlaIII CATG 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 366
PctI GAATGC 1 cut(s) 418
PdiI GCCGGC 2 cut(s) 52, 449
PdmI GAANNNNTTC 2 cut(s) 210, 533
PfeI GAWTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 5 cut(s) 29, 39, 223, 346, 469
Ppu21I YACGTR 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 166, 561
PspGI CCWGG 2 cut(s) 166, 561
PspPI GGNCC 2 cut(s) 164, 469
PspXI VCTCGAGB 1 cut(s) 377
PstI CTGCAG 1 cut(s) 559
RigI GGCCGGCC 1 cut(s) 54
RsaI GTAC 2 cut(s) 141, 255
RsaNI GTAC 2 cut(s) 140, 254
SacI GAGCTC 1 cut(s) 378
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 5 cut(s) 28, 38, 222, 345, 468
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 2 cut(s) 164, 469
ScrFI CCNGG 3 cut(s) 70, 168, 563
SduI GDGCHC 2 cut(s) 285, 378
SfaNI GCATC 1 cut(s) 460
SfcI CTRYAG 2 cut(s) 435, 555
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 377, 473, 487
SmoI CTYRAG 3 cut(s) 377, 473, 487
SpeI ACTAGT 1 cut(s) 158
SsiI CCGC 4 cut(s) 28, 58, 467, 500
SspMI CTAG 1 cut(s) 159
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 68, 166, 561
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 232
TaqI TCGA 1 cut(s) 378
TatI WGTACW 1 cut(s) 253
TauI GCSGC 2 cut(s) 30, 470
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 3 cut(s) 37, 221, 344
TspDTI ATGAA 5 cut(s) 219, 330, 476, 526, 540
Van91I CCANNNNNTGG 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 164
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XhoI CTCGAG 1 cut(s) 377
XmnI GAANNNNTTC 2 cut(s) 210, 533
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.