Rroxscaffold_4G00324320

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006776
Physical Location & Seq
Reverse (-)
55717384 .. 55721652
4269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324320.1

Sequence Viewer

Length: 594 bp
ATGCGAGAGAACGCCGGTCGCCGCCGTGGAAGCCCAGAGAACCAGCGGTGGGCGAGTTATCCCGTCCGGGCAAAAGCCATGTCACACGGGGGTGTTGCTTTTGAAAGTGATTCCTTCTTGAAGGTGGTGGGGTGTTGCTTGTTCAATATGCATATGTACAGAAATTCTGATGAGAGTGGCGATGCTGATTCTGAGAAGATTGTTGAGGTCCTTATGATTAACTCATCCAGTGGACCAGGTCTTTTATTTCCTAAGGGAGGCTGGGAGAATGATGAAACTGTGCAGGAAGCTGCCGTAAGAGAAGCTATTGAAGAAGCTGGGGTTCGAGGAGAATTAATGGATTTAGTGGGGTATTACCAATTTAAGAGTAAAACTCACCAGGATGAGTTAAACCCAGAAGGTATATGTAGAGCTGCTATGTTTGCATTGTTCGTCAAGGAGGAGCTTGAGTCATGGCCAGAACAGAGCACTCGAAACAGAACTTGGCTGACTGTTCAAGAGGCTACTGGTAGATGCCGGCATGCATGGATGAGTGAGGCCCTGGTGGAGGGCTTCTCAAAGTGGCATGCAGAGAAGATGATAGGTGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

197

Amino Acids

22.14

Weight (kDa)

5.34

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 70 - 168 3.9e-13 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 22, 46
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 49, 257, 547
AgsI TTSAA 5 cut(s) 104, 121, 145, 311, 497
AjnI CCWGG 3 cut(s) 235, 378, 540
AjuI GAANNNNNNNTTGG 2 cut(s) 466, 498
AleI CACNNNNGTG 1 cut(s) 90
AluBI AGCT 5 cut(s) 290, 305, 317, 413, 445
AluI AGCT 5 cut(s) 290, 305, 317, 413, 445
Alw21I GWGCWC 1 cut(s) 470
AoxI GGCC 2 cut(s) 455, 537
ApeKI GCWGC 2 cut(s) 290, 413
ApoI RAATTY 1 cut(s) 163
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AseI ATTAAT 1 cut(s) 335
AspS9I GGNCC 3 cut(s) 208, 233, 538
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 368
AvaII GGWCC 2 cut(s) 208, 233
AxyI CCTNAGG 1 cut(s) 252
BalI TGGCCA 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 2 cut(s) 277, 400
BceAI ACGGC 2 cut(s) 9, 278
BciT130I CCWGG 3 cut(s) 237, 380, 542
BcnI CCSGG 1 cut(s) 68
BisI GCNGC 3 cut(s) 22, 291, 414
BlsI GCNGC 3 cut(s) 23, 292, 415
Bme1390I CCNGG 4 cut(s) 68, 237, 380, 542
Bme18I GGWCC 2 cut(s) 208, 233
BmgT120I GGNCC 3 cut(s) 208, 233, 538
BmrFI CCNGG 4 cut(s) 68, 237, 380, 542
BmsI GCATC 2 cut(s) 172, 503
BplI GAGNNNNNCTC 4 cut(s) 358, 390, 539, 571
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 25, 540
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 257, 547
Bse118I RCCGGY 2 cut(s) 14, 516
Bse1I ACTGG 2 cut(s) 228, 511
Bse21I CCTNAGG 1 cut(s) 252
BseBI CCWGG 3 cut(s) 237, 380, 542
BseDI CCNNGG 2 cut(s) 25, 540
BseGI GGATG 3 cut(s) 224, 388, 534
BseLI CCNNNNNNNGG 3 cut(s) 49, 257, 547
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 2 cut(s) 228, 511
BseRI GAGGAG 2 cut(s) 342, 455
BseXI GCAGC 2 cut(s) 277, 400
BseYI CCCAGC 2 cut(s) 261, 317
BsgI GTGCAG 1 cut(s) 302
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 2 cut(s) 457, 539
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 470
BsiSI CCGG 3 cut(s) 15, 67, 517
BslI CCNNNNNNNGG 3 cut(s) 49, 257, 547
BsnI GGCC 2 cut(s) 457, 539
Bsp1286I GDGCHC 1 cut(s) 470
Bsp1407I TGTACA 1 cut(s) 156
BspACI CCGC 2 cut(s) 22, 46
BspANI GGCC 2 cut(s) 457, 539
BspCNI CTCAG 1 cut(s) 184
BsrFI RCCGGY 2 cut(s) 14, 516
BsrGI TGTACA 1 cut(s) 156
BsrI ACTGG 2 cut(s) 228, 511
BssAI RCCGGY 2 cut(s) 14, 516
BssECI CCNNGG 2 cut(s) 25, 540
Bst2UI CCWGG 3 cut(s) 237, 380, 542
Bst4CI ACNGT 2 cut(s) 280, 493
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 518, 522, 567
BstDEI CTNAG 2 cut(s) 192, 252
BstDSI CCRYGG 1 cut(s) 25
BstENI CCTNNNNNAGG 2 cut(s) 255, 545
BstF5I GGATG 3 cut(s) 224, 388, 534
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 2 cut(s) 30, 422
BstNI CCWGG 3 cut(s) 237, 380, 542
BstNSI RCATGY 2 cut(s) 524, 569
BstSCI CCNGG 4 cut(s) 66, 235, 378, 540
BstV1I GCAGC 2 cut(s) 277, 400
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 2 cut(s) 457, 539
BtgI CCRYGG 1 cut(s) 25
BtgZI GCGATG 1 cut(s) 195
BtsCI GGATG 3 cut(s) 224, 388, 534
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 3 cut(s) 518, 522, 567
Cfr10I RCCGGY 2 cut(s) 14, 516
Cfr13I GGNCC 3 cut(s) 208, 233, 538
CsiI ACCWGGT 1 cut(s) 235
Csp6I GTAC 1 cut(s) 157
CviAII CATG 5 cut(s) 79, 453, 521, 525, 566
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 2 cut(s) 192, 252
EaeI YGGCCR 1 cut(s) 455
Eco47I GGWCC 2 cut(s) 208, 233
Eco81I CCTNAGG 1 cut(s) 252
EcoNI CCTNNNNNAGG 2 cut(s) 255, 545
EcoO109I RGGNCCY 2 cut(s) 208, 538
EcoRII CCWGG 3 cut(s) 235, 378, 540
EcoT22I ATGCAT 2 cut(s) 153, 526
FaeI CATG 5 cut(s) 82, 456, 524, 528, 569
FatI CATG 5 cut(s) 78, 452, 520, 524, 565
FauNDI CATATG 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 22, 291, 414
FokI GGATG 3 cut(s) 211, 395, 541
Fsp4HI GCNGC 3 cut(s) 22, 291, 414
GluI GCNGC 3 cut(s) 22, 291, 414
GsaI CCCAGC 2 cut(s) 265, 321
HaeIII GGCC 2 cut(s) 457, 539
HapII CCGG 3 cut(s) 15, 67, 517
Hin1II CATG 5 cut(s) 82, 456, 524, 528, 569
HinfI GANTC 3 cut(s) 110, 188, 449
HpaII CCGG 3 cut(s) 15, 67, 517
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 1 cut(s) 233
Hpy188I TCNGA 2 cut(s) 169, 193
Hpy188III TCNNGA 2 cut(s) 118, 497
Hpy8I GTNNAC 1 cut(s) 233
HpyAV CCTTC 3 cut(s) 115, 124, 392
HpyCH4III ACNGT 2 cut(s) 280, 493
HpyCH4V TGCA 6 cut(s) 151, 283, 425, 524, 569, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 422
HpyF3I CTNAG 2 cut(s) 192, 252
Hsp92II CATG 5 cut(s) 82, 456, 524, 528, 569
KroI GCCGGC 1 cut(s) 516
KroNI GCCGGC 1 cut(s) 518
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 277, 400
LweI GCATC 2 cut(s) 172, 503
MabI ACCWGGT 1 cut(s) 235
MaeIII GTNAC 1 cut(s) 81
MboII GAAGA 3 cut(s) 208, 323, 586
MhlI GDGCHC 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 457
MluCI AATT 3 cut(s) 163, 332, 359
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 7 cut(s) 199, 251, 320, 433, 493, 529, 541
Mox20I TGGCCA 1 cut(s) 457
Mph1103I ATGCAT 2 cut(s) 153, 526
MroNI GCCGGC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 457
MseI TTAA 4 cut(s) 219, 335, 363, 389
MslI CAYNNNNRTG 2 cut(s) 90, 381
Msp20I TGGCCA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 46
MspI CCGG 3 cut(s) 15, 67, 517
MspR9I CCNGG 4 cut(s) 68, 237, 380, 542
MvaI CCWGG 3 cut(s) 237, 380, 542
MwoI GCNNNNNNNGC 2 cut(s) 30, 422
NaeI GCCGGC 1 cut(s) 518
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 153
NgoMIV GCCGGC 1 cut(s) 516
NlaIII CATG 5 cut(s) 82, 456, 524, 528, 569
NmuCI GTSAC 1 cut(s) 81
NsiI ATGCAT 2 cut(s) 153, 526
NspI RCATGY 2 cut(s) 524, 569
OliI CACNNNNGTG 1 cut(s) 90
PaeI GCATGC 2 cut(s) 524, 569
PdiI GCCGGC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 110, 188
PflFI GACNNNGTC 1 cut(s) 237
PkrI GCNGC 3 cut(s) 23, 292, 415
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 208
PshBI ATTAAT 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 3 cut(s) 235, 378, 540
PspFI CCCAGC 2 cut(s) 261, 317
PspGI CCWGG 3 cut(s) 235, 378, 540
PspPI GGNCC 3 cut(s) 208, 233, 538
PspPPI RGGWCCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 237
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 2 cut(s) 90, 381
SaqAI TTAA 4 cut(s) 219, 335, 363, 389
SatI GCNGC 3 cut(s) 22, 291, 414
Sau96I GGNCC 3 cut(s) 208, 233, 538
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 4 cut(s) 68, 237, 380, 542
SduI GDGCHC 1 cut(s) 470
SexAI ACCWGGT 1 cut(s) 235
SfaNI GCATC 2 cut(s) 172, 503
SinI GGWCC 2 cut(s) 208, 233
SmiMI CAYNNNNRTG 2 cut(s) 90, 381
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SphI GCATGC 2 cut(s) 524, 569
Sse9I AATT 3 cut(s) 163, 332, 359
SsiI CCGC 2 cut(s) 22, 46
StyD4I CCNGG 4 cut(s) 66, 235, 378, 540
TaaI ACNGT 2 cut(s) 280, 493
TaqI TCGA 2 cut(s) 325, 472
TasI AATT 3 cut(s) 163, 332, 359
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 2 cut(s) 110, 188
Tru1I TTAA 4 cut(s) 219, 335, 363, 389
Tru9I TTAA 4 cut(s) 219, 335, 363, 389
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 81
TseI GCWGC 2 cut(s) 290, 413
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 288
TspRI CASTG 1 cut(s) 235
Tth111I GACNNNGTC 1 cut(s) 237
VpaK11BI GGWCC 2 cut(s) 208, 233
VspI ATTAAT 1 cut(s) 335
XagI CCTNNNNNAGG 2 cut(s) 255, 545
XapI RAATTY 1 cut(s) 163
XceI RCATGY 2 cut(s) 524, 569
Zsp2I ATGCAT 2 cut(s) 153, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.