FvH4_7g03460

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006776
Physical Location & Seq
Reverse (-)
3812624 .. 3816116
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03460.t1

Sequence Viewer

Length: 525 bp
ATGTGTGATCTAGTGGCCCGTACGGGTCGACATCAGCAACGTTACGAGGCTGGCTGTCGTCTTGTCGCCGGGTGTATTCCATTCAGGTACAGAAATTCTGATGAGAGTGGCAATGCTGATTCTGAGAAGGTTGTTGAGGTCCTTATGATAAATTCATCTAGTGGACCAGGTCTTTTATTTCCTAAGGGAGGCTGGGAGAATGATGAAACTGTTCAGGAAGCTGCAGTAAGAGAAGCTATGGAAGAAGCTGGAGTTCGAGGAGAATTAATGGATTTAGTGGGGTATTATCAATTTAAGAGTAAAACTCACCAGGATGAGGTGAACCCAGAAGGTATATGTAGAGCTGCAATGTTTGCTTTGTTAGTCAAGGAGGAGCTTGAGTCGTGGCCGGAACAGAGCACTCGAAACCGAACTTGGCTGACTGTTGAAGAGGCTGCTGGTAGATGCAGGCATGCATGGATGAGTGAGGCCCTGGTGGACGGCTTTTCGAAGTGGCATGCAGAGAAGATGCTAGGTGCAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

175

Amino Acids

19.49

Weight (kDa)

5.1

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 47 - 145 1.6e-14 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 28
AclI AACGTT 1 cut(s) 40
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 2 cut(s) 94, 151
AfaI GTAC 2 cut(s) 22, 89
AfiI CCNNNNNNNGG 2 cut(s) 188, 316
AgsI TTSAA 1 cut(s) 428
AjnI CCWGG 3 cut(s) 166, 309, 471
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
AluBI AGCT 5 cut(s) 221, 236, 248, 344, 376
AluI AGCT 5 cut(s) 221, 236, 248, 344, 376
Alw21I GWGCWC 1 cut(s) 401
AoxI GGCC 3 cut(s) 15, 386, 468
ApeKI GCWGC 3 cut(s) 221, 344, 434
ApoI RAATTY 2 cut(s) 94, 151
AseI ATTAAT 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 210
AspS9I GGNCC 4 cut(s) 16, 139, 164, 469
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 299, 331
AsuII TTCGAA 1 cut(s) 488
AvaII GGWCC 2 cut(s) 139, 164
AxyI CCTNAGG 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 208, 331, 421
BceAI ACGGC 1 cut(s) 496
BciT130I CCWGG 3 cut(s) 168, 311, 473
BcnI CCSGG 1 cut(s) 70
BfaI CTAG 3 cut(s) 11, 159, 512
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 3 cut(s) 222, 345, 435
BlsI GCNGC 3 cut(s) 223, 346, 436
Bme1390I CCNGG 4 cut(s) 70, 168, 311, 473
Bme18I GGWCC 2 cut(s) 139, 164
BmgT120I GGNCC 4 cut(s) 16, 139, 164, 469
BmrFI CCNGG 4 cut(s) 70, 168, 311, 473
BmsI GCATC 2 cut(s) 434, 498
BplI GAGNNNNNCTC 2 cut(s) 289, 321
BpmI CTGGAG 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 70
BsaJI CCNNGG 1 cut(s) 471
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 316
Bse21I CCTNAGG 1 cut(s) 183
Bse3DI GCAATG 2 cut(s) 118, 354
BseBI CCWGG 3 cut(s) 168, 311, 473
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 2 cut(s) 319, 465
BseLI CCNNNNNNNGG 2 cut(s) 188, 316
BseMI GCAATG 2 cut(s) 118, 354
BseMII CTCAG 1 cut(s) 114
BseRI GAGGAG 2 cut(s) 273, 386
BseXI GCAGC 3 cut(s) 208, 331, 421
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 3 cut(s) 17, 388, 470
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 2 cut(s) 69, 389
BsiWI CGTACG 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 188, 316
BsnI GGCC 3 cut(s) 17, 388, 470
Bsp119I TTCGAA 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 401
Bsp143I GATC 1 cut(s) 7
BspANI GGCC 3 cut(s) 17, 388, 470
BspCNI CTCAG 1 cut(s) 115
BspMAI CTGCAG 1 cut(s) 226
BspT104I TTCGAA 1 cut(s) 488
BsrDI GCAATG 2 cut(s) 118, 354
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 1 cut(s) 7
Bst2UI CCWGG 3 cut(s) 168, 311, 473
Bst4CI ACNGT 2 cut(s) 211, 424
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 488
BstC8I GCNNGC 4 cut(s) 52, 449, 453, 498
BstDEI CTNAG 2 cut(s) 123, 183
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 319, 465
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 3 cut(s) 168, 311, 473
BstNSI RCATGY 2 cut(s) 455, 500
BstSCI CCNGG 4 cut(s) 68, 166, 309, 471
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 3 cut(s) 208, 331, 421
Bsu36I CCTNAGG 1 cut(s) 183
BsuRI GGCC 3 cut(s) 17, 388, 470
BtsCI GGATG 2 cut(s) 319, 465
Cac8I GCNNGC 4 cut(s) 52, 449, 453, 498
Cfr13I GGNCC 4 cut(s) 16, 139, 164, 469
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 2 cut(s) 21, 88
CviAII CATG 3 cut(s) 452, 456, 497
CviQI GTAC 2 cut(s) 21, 88
DdeI CTNAG 2 cut(s) 123, 183
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 139, 164
Eco81I CCTNAGG 1 cut(s) 183
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoO109I RGGNCCY 2 cut(s) 139, 469
EcoRII CCWGG 3 cut(s) 166, 309, 471
EcoT22I ATGCAT 1 cut(s) 457
FaeI CATG 3 cut(s) 455, 459, 500
FaiI YATR 7 cut(s) 146, 239, 335, 337, 453, 457, 498
FatI CATG 3 cut(s) 451, 455, 496
FblI GTMKAC 1 cut(s) 28
Fnu4HI GCNGC 3 cut(s) 222, 345, 435
FokI GGATG 2 cut(s) 326, 472
Fsp4HI GCNGC 3 cut(s) 222, 345, 435
FspBI CTAG 3 cut(s) 11, 159, 512
GluI GCNGC 3 cut(s) 222, 345, 435
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 3 cut(s) 17, 388, 470
HapII CCGG 2 cut(s) 69, 389
Hin1II CATG 3 cut(s) 455, 459, 500
HincII GTYRAC 1 cut(s) 29
HindII GTYRAC 1 cut(s) 29
HinfI GANTC 2 cut(s) 119, 380
HpaII CCGG 2 cut(s) 69, 389
HphI GGTGA 2 cut(s) 299, 331
Hpy166II GTNNAC 4 cut(s) 29, 164, 322, 478
Hpy188I TCNGA 2 cut(s) 100, 124
Hpy188III TCNNGA 1 cut(s) 215
Hpy8I GTNNAC 4 cut(s) 29, 164, 322, 478
HpyAV CCTTC 2 cut(s) 121, 323
HpyCH4III ACNGT 2 cut(s) 211, 424
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 6 cut(s) 224, 347, 447, 455, 500, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 2 cut(s) 123, 183
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 3 cut(s) 455, 459, 500
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 3 cut(s) 208, 331, 421
LweI GCATC 2 cut(s) 434, 498
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 3 cut(s) 11, 159, 512
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 1 cut(s) 41
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 3 cut(s) 254, 440, 517
MhlI GDGCHC 1 cut(s) 401
MluCI AATT 4 cut(s) 94, 151, 263, 290
MlyI GAGTC 1 cut(s) 389
MnlI CCTC 8 cut(s) 40, 130, 182, 251, 310, 364, 424, 460
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 2 cut(s) 266, 294
MslI CAYNNNNRTG 1 cut(s) 312
MspI CCGG 2 cut(s) 69, 389
MspR9I CCNGG 4 cut(s) 70, 168, 311, 473
MvaI CCWGG 3 cut(s) 168, 311, 473
MwoI GCNNNNNNNGC 1 cut(s) 353
NciI CCSGG 1 cut(s) 70
NdeII GATC 1 cut(s) 7
NlaIII CATG 3 cut(s) 455, 459, 500
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 2 cut(s) 455, 500
NspV TTCGAA 1 cut(s) 488
PaeI GCATGC 2 cut(s) 455, 500
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 119
Pfl23II CGTACG 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 3 cut(s) 223, 346, 436
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PpuMI RGGWCCY 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 266
Psp1406I AACGTT 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 166, 309, 471
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 3 cut(s) 166, 309, 471
PspLI CGTACG 1 cut(s) 20
PspPI GGNCC 4 cut(s) 16, 139, 164, 469
PspPPI RGGWCCY 1 cut(s) 139
PstI CTGCAG 1 cut(s) 226
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 2 cut(s) 22, 89
RsaNI GTAC 2 cut(s) 21, 88
RseI CAYNNNNRTG 1 cut(s) 312
SalI GTCGAC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 266, 294
SatI GCNGC 3 cut(s) 222, 345, 435
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 4 cut(s) 16, 139, 164, 469
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 4 cut(s) 70, 168, 311, 473
SduI GDGCHC 1 cut(s) 401
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 2 cut(s) 434, 498
SfcI CTRYAG 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 488
SinI GGWCC 2 cut(s) 139, 164
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 2 cut(s) 455, 500
Sse9I AATT 4 cut(s) 94, 151, 263, 290
SspMI CTAG 3 cut(s) 11, 159, 512
StyD4I CCNGG 4 cut(s) 68, 166, 309, 471
TaaI ACNGT 2 cut(s) 211, 424
TaiI ACGT 1 cut(s) 43
TaqI TCGA 4 cut(s) 28, 256, 403, 488
TasI AATT 4 cut(s) 94, 151, 263, 290
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 2 cut(s) 266, 294
Tru9I TTAA 2 cut(s) 266, 294
TseI GCWGC 3 cut(s) 221, 344, 434
TspDTI ATGAA 2 cut(s) 144, 219
Tth111I GACNNNGTC 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 139, 164
VspI ATTAAT 1 cut(s) 266
XagI CCTNNNNNAGG 1 cut(s) 186
XapI RAATTY 2 cut(s) 94, 151
XceI RCATGY 2 cut(s) 455, 500
XmiI GTMKAC 1 cut(s) 28
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 3 cut(s) 11, 159, 512
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.