Rorug01G0054800

Nudix hydrolase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006776
Physical Location & Seq
Forward (+)
9026336 .. 9026599
264 bp
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UTR
Exon/CDS
Intron
Rorug01G0054800.1

Sequence Viewer

Length: 264 bp
ATGATTCATCTGAGTCGCCTCGAGTACCACATCAGAGAAGAGCTCAACAAAACAGCTCCTCGCACAATGGTTGTGCTTGATCCTGTAAAGGTCGTCATCACCAACTGGAAAGATGACCTCATAAAGGACTGTGAAGCAAGAAAGTGGCCCGATTCTGAGGCAGAGTCCTACAAGGTTCCAATTTCCAAGGTGTTTGATGCGGGAAGCGTGAACACAATAGTACGAGGCTCCGTCAAGCGTCGAAAACCATATGAGATGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

87

Amino Acids

10.12

Weight (kDa)

9.13

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c_C PF03950 19 - 63 7.1e-08 tRNA synthetases class I (E and Q), anti-codon binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 200
AclWI GGATC 1 cut(s) 74
AfaI GTAC 2 cut(s) 26, 222
AfiI CCNNNNNNNGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 43, 56, 261
AluI AGCT 3 cut(s) 43, 56, 261
Alw21I GWGCWC 1 cut(s) 45
AlwI GGATC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 20
BanII GRGCYC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 45
BfaI CTAG 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 2 cut(s) 177, 229
BmsI GCATC 1 cut(s) 187
BplI GAGNNNNNCTC 2 cut(s) 27, 59
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 186
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 1 cut(s) 48
BshFI GGCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 124
BsnI GGCC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 1 cut(s) 200
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 1 cut(s) 148
BspLI GGNNCC 2 cut(s) 177, 229
BspPI GGATC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 33
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 33
BstDEI CTNAG 2 cut(s) 11, 156
BstENI CCTNNNNNAGG 1 cut(s) 122
BstKTI GATC 1 cut(s) 82
BstMBI GATC 1 cut(s) 79
BsuRI GGCC 1 cut(s) 148
Cfr13I GGNCC 1 cut(s) 147
CseI GACGC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 25, 221
CviJI RGCY 5 cut(s) 43, 56, 148, 228, 261
CviKI_1 RGCY 5 cut(s) 43, 56, 148, 228, 261
CviQI GTAC 2 cut(s) 25, 221
DdeI CTNAG 2 cut(s) 11, 156
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 186
FaiI YATR 3 cut(s) 122, 250, 252
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 1 cut(s) 250
FriOI GRGCYC 1 cut(s) 45
FspBI CTAG 1 cut(s) 262
HaeIII GGCC 1 cut(s) 148
HgaI GACGC 1 cut(s) 227
HinfI GANTC 4 cut(s) 4, 13, 152, 164
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 12, 35, 157
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 243
HpyCH4III ACNGT 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 11, 156
Kzo9I GATC 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 61, 233
LpnPI CCDG 2 cut(s) 91, 96
LweI GCATC 1 cut(s) 187
MaeI CTAG 1 cut(s) 262
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 1 cut(s) 180
MlyI GAGTC 2 cut(s) 22, 173
MnlI CCTC 5 cut(s) 29, 69, 128, 151, 218
NdeI CATATG 1 cut(s) 250
NdeII GATC 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 177, 229
PaeR7I CTCGAG 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 4, 152
PleI GAGTC 2 cut(s) 21, 172
PpsI GAGTC 2 cut(s) 21, 172
Psp124BI GAGCTC 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 177, 229
PspPI GGNCC 1 cut(s) 147
PspXI VCTCGAGB 1 cut(s) 20
RsaI GTAC 2 cut(s) 26, 222
RsaNI GTAC 2 cut(s) 25, 221
SacI GAGCTC 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 33
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 2 cut(s) 22, 173
SduI GDGCHC 1 cut(s) 45
SetI ASST 7 cut(s) 45, 58, 93, 120, 177, 192, 263
SfaNI GCATC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 1 cut(s) 180
SsiI CCGC 1 cut(s) 200
SspMI CTAG 1 cut(s) 262
SstI GAGCTC 1 cut(s) 45
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 131
TaqI TCGA 2 cut(s) 21, 241
TasI AATT 1 cut(s) 180
TfiI GAWTC 2 cut(s) 4, 152
TspGWI ACGGA 1 cut(s) 220
XagI CCTNNNNNAGG 1 cut(s) 122
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.