AT3G12880
ERF Family

Plant invertase pectin methylesterase inhibitor superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013280
Physical Location & Seq
Forward (+)
4095414 .. 4096236
823 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12880.1

Sequence Viewer

Length: 540 bp
ATGAACAAAAAAATGGCTTTGCTTGCAAAACTTCTTATCCTCACCACACTTGTAACAACAATATCAGTAATATCCAGAGCCAATGAAGAGCTAATGATGCAACAATGTCACAACTCTGACAATCCCACCTTGTGTCTTCGCTGTCTAAGCTCCGACCCTGGTTCTCATGAGGCCGACAAAGTAGGACTGGCTCGTATCATCCTCAAATGCATCAACTCACACCTCCTTGTTCTCACCAAGAACGCTTCCACTTTGGGCTCGCAGCACTACCAGAATCCAAATACTGCAGCAGCGTTGAAGCAGTGCGGTTTGGGTTTCTCAACGGCTAAGCATGGCGTGGGAGAGGCCGACACTCAACTTATAACCGGAGATTACGACAAGGCTGCGTATGACGTCAGCGTGAAGGTGGTGAATCCTCCGGTATCGTGCCGCAACAGTCTCGAGGCACTCAACATTCAAGTCCCTTCAAGTTTCCGTTACCATATGGATGTATACTTGGCGTTGACTCAAGCTCTCCTCAGGATCGTTGATCGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

179

Amino Acids

19.54

Weight (kDa)

8.54

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 34 - 154 4.9e-09 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AatII GACGTC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 492
AciI CCGC 2 cut(s) 306, 430
AclWI GGATC 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 132
AgsI TTSAA 3 cut(s) 298, 458, 468
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 3 cut(s) 91, 150, 512
AluI AGCT 3 cut(s) 91, 150, 512
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 2 cut(s) 171, 345
ApeKI GCWGC 4 cut(s) 262, 287, 290, 383
AsuHPI GGTGA 3 cut(s) 34, 226, 421
AvaI CYCGRG 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 518
BanII GRGCYC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 128
BbvI GCAGC 4 cut(s) 274, 299, 302, 370
BceAI ACGGC 1 cut(s) 339
BcgI CGANNNNNNTGC 4 cut(s) 365, 399, 421, 455
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 1 cut(s) 538
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 5 cut(s) 263, 288, 291, 384, 430
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 5 cut(s) 264, 289, 292, 385, 431
Bme1390I CCNGG 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 440
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 87, 219
BpiI GAAGAC 1 cut(s) 128
Bpu1102I GCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 2 cut(s) 365, 418
Bse1I ACTGG 1 cut(s) 192
Bse21I CCTNAGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 2 cut(s) 198, 493
BseMII CTCAG 1 cut(s) 532
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 4 cut(s) 274, 299, 302, 370
BshFI GGCC 2 cut(s) 173, 347
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 2 cut(s) 366, 419
BslFI GGGAC 1 cut(s) 446
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 2 cut(s) 173, 347
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 2 cut(s) 522, 529
Bsp1720I GCTNAGC 1 cut(s) 327
BspACI CCGC 2 cut(s) 306, 430
BspANI GGCC 2 cut(s) 173, 347
BspCNI CTCAG 1 cut(s) 531
BspHI TCATGA 1 cut(s) 166
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 81
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 2 cut(s) 522, 529
BssNAI GTATAC 1 cut(s) 493
BssNI GRCGYC 1 cut(s) 393
Bst1107I GTATAC 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 437
Bst6I CTCTTC 1 cut(s) 81
BstACI GRCGYC 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 24, 260
BstDEI CTNAG 3 cut(s) 146, 327, 518
BstF5I GGATG 2 cut(s) 198, 493
BstKTI GATC 2 cut(s) 525, 532
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 522, 529
BstMWI GCNNNNNNNGC 3 cut(s) 23, 97, 147
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 4 cut(s) 274, 299, 302, 370
BstV2I GAAGAC 1 cut(s) 128
BstZ17I GTATAC 1 cut(s) 493
Bsu36I CCTNAGG 1 cut(s) 518
BsuRI GGCC 2 cut(s) 173, 347
BtsCI GGATG 2 cut(s) 198, 493
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 2 cut(s) 24, 260
CciI TCATGA 1 cut(s) 166
CviAII CATG 2 cut(s) 167, 332
DdeI CTNAG 3 cut(s) 146, 327, 518
DpnI GATC 2 cut(s) 524, 531
DpnII GATC 2 cut(s) 522, 529
DraIII CACNNNGTG 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 260
Eco81I CCTNAGG 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 157
EcoT22I ATGCAT 1 cut(s) 212
EcoT38I GRGCYC 1 cut(s) 260
FaeI CATG 2 cut(s) 170, 335
FaiI YATR 7 cut(s) 168, 333, 362, 390, 483, 485, 493
FaqI GGGAC 1 cut(s) 446
FatI CATG 2 cut(s) 166, 331
FauNDI CATATG 1 cut(s) 483
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 5 cut(s) 263, 288, 291, 384, 430
FokI GGATG 2 cut(s) 185, 500
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 5 cut(s) 263, 288, 291, 384, 430
FspBI CTAG 1 cut(s) 538
GluI GCNGC 5 cut(s) 263, 288, 291, 384, 430
HaeIII GGCC 2 cut(s) 173, 347
HapII CCGG 2 cut(s) 366, 419
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 2 cut(s) 170, 335
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 3 cut(s) 274, 412, 505
HpaII CCGG 2 cut(s) 366, 419
HphI GGTGA 3 cut(s) 34, 226, 421
Hpy166II GTNNAC 2 cut(s) 493, 504
Hpy188I TCNGA 2 cut(s) 118, 154
Hpy188III TCNNGA 4 cut(s) 75, 167, 440, 520
Hpy8I GTNNAC 2 cut(s) 493, 504
HpyAV CCTTC 2 cut(s) 397, 474
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 4 cut(s) 26, 100, 210, 287
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 97, 147
HpyF3I CTNAG 3 cut(s) 146, 327, 518
HpySE526I ACGT 1 cut(s) 393
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 2 cut(s) 170, 335
Kzo9I GATC 2 cut(s) 522, 529
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 155
LpnPI CCDG 8 cut(s) 88, 144, 171, 173, 284, 379, 432, 505
Lsp1109I GCAGC 4 cut(s) 274, 299, 302, 370
LweI GCATC 2 cut(s) 87, 219
MaeI CTAG 1 cut(s) 538
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 52, 107, 476
MalI GATC 2 cut(s) 524, 531
MboI GATC 2 cut(s) 522, 529
MboII GAAGA 2 cut(s) 98, 128
MhlI GDGCHC 1 cut(s) 260
MlyI GAGTC 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 8 cut(s) 50, 163, 212, 233, 337, 426, 436, 527
Mph1103I ATGCAT 1 cut(s) 212
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 2 cut(s) 366, 419
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 23, 97, 147
NdeI CATATG 1 cut(s) 483
NdeII GATC 2 cut(s) 522, 529
NlaIII CATG 2 cut(s) 170, 335
NmuCI GTSAC 1 cut(s) 107
NsiI ATGCAT 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 440
PagI TCATGA 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 274, 412
PkrI GCNGC 5 cut(s) 264, 289, 292, 385, 431
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PsiI TTATAA 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PstI CTGCAG 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 486
SapI GCTCTTC 1 cut(s) 81
SatI GCNGC 5 cut(s) 263, 288, 291, 384, 430
Sau3AI GATC 2 cut(s) 522, 529
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 260
SetI ASST 7 cut(s) 93, 131, 152, 225, 396, 408, 514
SfaNI GCATC 2 cut(s) 87, 219
SfcI CTRYAG 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 2 cut(s) 440, 507
SmoI CTYRAG 2 cut(s) 440, 507
SsiI CCGC 2 cut(s) 306, 430
SspMI CTAG 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 441
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 2 cut(s) 274, 412
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 4 cut(s) 262, 287, 290, 383
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 2 cut(s) 17, 99
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 1 cut(s) 308
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 1 cut(s) 538
ZraI GACGTC 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.