RLG00000032898
ERF Family

Plant invertase pectin methylesterase inhibitor superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013280
Physical Location & Seq
Forward (+)
20589559 .. 20590035
477 bp
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UTR
Exon/CDS
Intron
RLM00000032898

Sequence Viewer

Length: 477 bp
ATGGCTTCTTCTTTCACAATTGTTCTTCTAGTTCTTGCTATAATCCCGTGTTTTATTGTCATTGCAGCTGACCAACTAATCCAAATCGAGTGTCACAACGCCGAGGTACCAACAACCTGCATCGAGTGCCTGAAATCCGACCCACGCAGCGCGAAGGCTAACAAAGCTGGGATTGCCGTCATTATCCTAAGTTCTCTAAGCAACAATGCCAAGACTTTGGCCACCAACATGTCAGAAATAGCTTCTGGAATCAAAGATAAAAATGTAGCAAAGGGTTTTACTCGTCCACACAGGACTTTGGCTTCGGCAACCCTCAAGTTGAAGAGTGGCAAGTATGGTGGTGCTGAACATTCTGTGAGTCGAGCTGTCGTGTATGTGGCTGATTGTCGTCATGAGATTGAGGTTTACAATGGGAAAGTAGTTCCTAGAGACATGATTTATGAAATGTTGATATATGAAGAGCTATCAGGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

159

Amino Acids

17.06

Weight (kDa)

8.21

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 26 - 133 7.5e-06 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 1 cut(s) 106
AccII CGCG 1 cut(s) 152
AcoI YGGCCR 1 cut(s) 219
AfaI GTAC 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 5 cut(s) 68, 167, 242, 365, 463
AluI AGCT 5 cut(s) 68, 167, 242, 365, 463
Alw26I GTCTC 1 cut(s) 423
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 3 cut(s) 65, 147, 471
ArsI GACNNNNNNTTYG 4 cut(s) 76, 108, 286, 318
Asp718I GGTACC 1 cut(s) 106
AspLEI GCGC 1 cut(s) 152
BalI TGGCCA 1 cut(s) 221
BanI GGYRCC 1 cut(s) 106
BbvI GCAGC 3 cut(s) 77, 159, 458
BceAI ACGGC 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 2 cut(s) 29, 426
BfuAI ACCTGC 1 cut(s) 125
BisI GCNGC 3 cut(s) 66, 148, 472
BlsI GCNGC 3 cut(s) 67, 149, 473
BmiI GGNNCC 1 cut(s) 108
BmsI GCATC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 102
BseMI GCAATG 1 cut(s) 60
BseXI GCAGC 3 cut(s) 77, 159, 458
BseYI CCCAGC 1 cut(s) 167
Bsh1236I CGCG 1 cut(s) 152
BshFI GGCC 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 423
BsnI GGCC 1 cut(s) 221
BspANI GGCC 1 cut(s) 221
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 108
BspMI ACCTGC 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 102
Bst6I CTCTTC 2 cut(s) 317, 453
BstAPI GCANNNNNTGC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 188, 197
BstFNI CGCG 1 cut(s) 152
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 1 cut(s) 423
BstMWI GCNNNNNNNGC 3 cut(s) 126, 164, 173
BstNSI RCATGY 1 cut(s) 232
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 3 cut(s) 77, 159, 458
BstXI CCANNNNNNTGG 1 cut(s) 217
BsuRI GGCC 1 cut(s) 221
BveI ACCTGC 1 cut(s) 125
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 152
Csp6I GTAC 1 cut(s) 107
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 3 cut(s) 229, 392, 433
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 188, 197
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 317, 453
EarI CTCTTC 2 cut(s) 317, 453
FaeI CATG 3 cut(s) 232, 395, 436
FaiI YATR 9 cut(s) 41, 230, 336, 375, 393, 434, 441, 454, 456
FatI CATG 3 cut(s) 228, 391, 432
Fnu4HI GCNGC 3 cut(s) 66, 148, 472
Fsp4HI GCNGC 3 cut(s) 66, 148, 472
FspBI CTAG 2 cut(s) 29, 426
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 66, 148, 472
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 1 cut(s) 221
HhaI GCGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 232, 395, 436
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 2 cut(s) 249, 358
Hpy166II GTNNAC 2 cut(s) 287, 406
Hpy188I TCNGA 2 cut(s) 139, 235
Hpy188III TCNNGA 2 cut(s) 246, 392
Hpy8I GTNNAC 2 cut(s) 287, 406
HpyAV CCTTC 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 65, 120
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 164, 173
HpyF3I CTNAG 2 cut(s) 188, 197
Hsp92II CATG 3 cut(s) 232, 395, 436
HspAI GCGC 1 cut(s) 150
KpnI GGTACC 1 cut(s) 110
LguI GCTCTTC 1 cut(s) 453
LpnPI CCDG 6 cut(s) 130, 143, 153, 231, 277, 453
Lsp1109I GCAGC 3 cut(s) 77, 159, 458
LweI GCATC 1 cut(s) 129
MaeI CTAG 2 cut(s) 29, 426
MaeIII GTNAC 1 cut(s) 92
MboII GAAGA 3 cut(s) 17, 334, 470
MfeI CAATTG 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 1 cut(s) 18
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 3 cut(s) 97, 323, 394
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 227
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 68
MunI CAATTG 1 cut(s) 18
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 126, 164, 173
NlaIII CATG 3 cut(s) 232, 395, 436
NlaIV GGNNCC 1 cut(s) 108
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 92
NspI RCATGY 1 cut(s) 232
PagI TCATGA 1 cut(s) 391
PciI ACATGT 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 3 cut(s) 67, 149, 473
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PscI ACATGT 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 453
SatI GCNGC 3 cut(s) 66, 148, 472
SchI GAGTC 1 cut(s) 367
SetI ASST 8 cut(s) 70, 108, 119, 169, 244, 367, 405, 465
SfaNI GCATC 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 1 cut(s) 18
SspMI CTAG 2 cut(s) 29, 426
TaqI TCGA 3 cut(s) 87, 123, 361
TasI AATT 1 cut(s) 18
TfiI GAWTC 1 cut(s) 249
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 3 cut(s) 65, 147, 471
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 456, 471
XceI RCATGY 1 cut(s) 232
XspI CTAG 2 cut(s) 29, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.