Rh5CG198500
ERF Family

Plant invertase pectin methylesterase inhibitor superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013280
Physical Location & Seq
Forward (+)
19862328 .. 19863321
994 bp
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UTR
Exon/CDS
Intron
Rh5CG198500.1

Sequence Viewer

Length: 453 bp
ATGGCAACTGACCGACTAATCCAAATCGAGTGTCACAACGCCGAGGTACCAACATCCTGCATCGAATGCCTGAAATCTGACCCACGCAGCGCGAAGGCTAACAAAGCTGGGATTGCCGTCATTATCCTGAGTTCTCTAAGCAACAATGCCAAGACAGTCAAGACTTTGGCCATCAACATGTCAGAAATAGCTTCTGGAATCAAAGATAAAAATGTCACAAAAGTTTTGAAAACATGTAGCAAAGAGTTTTACTCATCCACACAGGACTTGGCTTCGGCAACCCTCAAGTTGAAAAGTGGCAGGTATGGTGGTGCTGAACATTCTGTGAGTCGAGCTGTCGTGTATGTGGCTTATTGTCGTCATGAGATTGATGGGAAAGTAGTTCCTAGAGACATGATTTATGAAATGTTGATATTATCCGAGGCTGCTAATAGAATAATTGAAGGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

150

Amino Acids

16.29

Weight (kDa)

8.8

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 6 - 127 2.9e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 291
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccII CGCG 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 168
AfaI GTAC 1 cut(s) 48
AflIII ACRYGT 2 cut(s) 177, 233
AgsI TTSAA 3 cut(s) 229, 292, 443
AluBI AGCT 3 cut(s) 107, 191, 335
AluI AGCT 3 cut(s) 107, 191, 335
Alw26I GTCTC 1 cut(s) 384
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 87, 425
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
Asp718I GGTACC 1 cut(s) 46
AspLEI GCGC 1 cut(s) 92
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 1 cut(s) 46
BbvI GCAGC 2 cut(s) 99, 412
BccI CCATC 2 cut(s) 179, 365
BceAI ACGGC 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 2 cut(s) 387, 451
BfuAI ACCTGC 1 cut(s) 291
BisI GCNGC 2 cut(s) 88, 426
BlsI GCNGC 2 cut(s) 89, 427
BmiI GGNNCC 1 cut(s) 48
BmsI GCATC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpuEI CTTGAG 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 42, 420
BseDI CCNNGG 2 cut(s) 42, 420
BseGI GGATG 2 cut(s) 53, 254
BseMII CTCAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 99, 412
BseYI CCCAGC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 1 cut(s) 170
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 291
BspT107I GGYRCC 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 42, 420
Bst4CI ACNGT 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 66
BstDEI CTNAG 2 cut(s) 128, 137
BstF5I GGATG 2 cut(s) 53, 254
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 92
BstMAI GTCTC 1 cut(s) 384
BstMWI GCNNNNNNNGC 3 cut(s) 66, 104, 113
BstNSI RCATGY 2 cut(s) 181, 237
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 99, 412
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 2 cut(s) 53, 254
BveI ACCTGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 361
CfoI GCGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 47
CviAII CATG 4 cut(s) 178, 234, 362, 394
CviJI RGCY 8 cut(s) 98, 107, 170, 191, 272, 335, 350, 425
CviKI_1 RGCY 8 cut(s) 98, 107, 170, 191, 272, 335, 350, 425
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 2 cut(s) 128, 137
EaeI YGGCCR 1 cut(s) 168
FaeI CATG 4 cut(s) 181, 237, 365, 397
FaiI YATR 7 cut(s) 179, 235, 306, 345, 363, 395, 402
FatI CATG 4 cut(s) 177, 233, 361, 393
Fnu4HI GCNGC 2 cut(s) 88, 426
FokI GGATG 2 cut(s) 40, 241
Fsp4HI GCNGC 2 cut(s) 88, 426
FspBI CTAG 2 cut(s) 387, 451
GlaI GCGC 1 cut(s) 91
GluI GCNGC 2 cut(s) 88, 426
GsaI CCCAGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 170
HhaI GCGC 1 cut(s) 92
Hin1II CATG 4 cut(s) 181, 237, 365, 397
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 3 cut(s) 198, 328, 447
Hpy188I TCNGA 3 cut(s) 79, 184, 421
Hpy188III TCNNGA 4 cut(s) 127, 160, 195, 362
HpyAV CCTTC 2 cut(s) 88, 437
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4V TGCA 1 cut(s) 60
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 104, 113
HpyF3I CTNAG 2 cut(s) 128, 137
Hsp92II CATG 4 cut(s) 181, 237, 365, 397
HspAI GCGC 1 cut(s) 90
KpnI GGTACC 1 cut(s) 50
LpnPI CCDG 7 cut(s) 70, 83, 93, 140, 180, 248, 286
Lsp1109I GCAGC 2 cut(s) 99, 412
LweI GCATC 1 cut(s) 69
MaeI CTAG 2 cut(s) 387, 451
MaeIII GTNAC 2 cut(s) 32, 214
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 170
MlyI GAGTC 2 cut(s) 337, 441
MnlI CCTC 3 cut(s) 37, 293, 415
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 71
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 66, 104, 113
NlaIII CATG 4 cut(s) 181, 237, 365, 397
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 32, 214
NspI RCATGY 2 cut(s) 181, 237
PagI TCATGA 1 cut(s) 361
PciI ACATGT 2 cut(s) 177, 233
PctI GAATGC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 2 cut(s) 89, 427
PleI GAGTC 2 cut(s) 336, 441
PpsI GAGTC 2 cut(s) 336, 441
PscI ACATGT 2 cut(s) 177, 233
PspFI CCCAGC 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 48
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 176
SatI GCNGC 2 cut(s) 88, 426
SchI GAGTC 2 cut(s) 337, 441
SetI ASST 5 cut(s) 48, 109, 193, 305, 337
SfaNI GCATC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 1 cut(s) 438
SspMI CTAG 2 cut(s) 387, 451
TaaI ACNGT 1 cut(s) 157
TaqI TCGA 3 cut(s) 27, 63, 331
TaqII GACCGA 1 cut(s) 27
TasI AATT 1 cut(s) 438
TfiI GAWTC 1 cut(s) 198
TseFI GTSAC 2 cut(s) 32, 214
TseI GCWGC 2 cut(s) 87, 425
Tsp45I GTSAC 2 cut(s) 32, 214
TspDTI ATGAA 1 cut(s) 417
XceI RCATGY 2 cut(s) 181, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 2 cut(s) 387, 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.