AT4G15750

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013280
Physical Location & Seq
Reverse (-)
8967519 .. 8968615
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G15750.1

Sequence Viewer

Length: 516 bp
ATGACTAATCCCATGATCATGGTTATGCTGTTGTTGTTTCTTGTGATGTCGACTAGAGCAGACGAAGAGCTGATTAAGACAGAGTGTAATCACACAGAATACCAAAACGTATGCCTCTTCTGTCTTGAAGCCGATCCAATCTCCTTCAATATCGACCGTGCTGGACTTGTCAACATCATTATACACTGTCTCGGATCTCAACTTGATGTTCTTATCAACACCGTCACGAGTCTAAAGTTGATGAAAGGAGAGGGTGAAGCAAATGAGAATGTTCTGAAAGATTGCGTCACAGGCTTTGCGATTGCACAATTACGACTTCAAGGAGCCAACATCGATTTGATAACCCTTAATTACGATAAAGCGTACGAATTGGTGAAAACTGCGTTAAACTATCCTCGGACTTGCGAAGAAAATCTCCAAAAACTCAAGTTCAAAGATTCATCTGATGTTTATGACGATATCTTGGCATATAGCCAACTCACCTCTGTTGCTAAGACGTTGATCCACCGTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

171

Amino Acids

19.27

Weight (kDa)

4.91

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 24 - 164 1.8e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 50
AclWI GGATC 3 cut(s) 128, 202, 496
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 4 cut(s) 128, 148, 320, 433
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 3 cut(s) 128, 202, 496
AsuHPI GGTGA 3 cut(s) 266, 385, 472
BauI CACGAG 1 cut(s) 226
BclI TGATCA 1 cut(s) 15
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 2 cut(s) 54, 514
BmiI GGNNCC 1 cut(s) 325
BpuEI CTTGAG 1 cut(s) 410
Bsa29I ATCGAT 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 395
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 395
Bsh1285I CGRYCG 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 157
BsiWI CGTACG 1 cut(s) 363
BsmAI GTCTC 1 cut(s) 194
Bsp143I GATC 4 cut(s) 15, 133, 194, 501
BspDI ATCGAT 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 3 cut(s) 128, 202, 496
BspQI GCTCTTC 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 4 cut(s) 15, 133, 194, 501
BssSI CACGAG 1 cut(s) 226
Bst2BI CACGAG 1 cut(s) 226
Bst4CI ACNGT 4 cut(s) 158, 188, 223, 509
Bst6I CTCTTC 2 cut(s) 60, 122
BstDEI CTNAG 1 cut(s) 492
BstKTI GATC 4 cut(s) 18, 136, 197, 504
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 4 cut(s) 15, 133, 194, 501
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstX2I RGATCY 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 19
BstYI RGATCY 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 333
BsuTUI ATCGAT 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 184
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 1 cut(s) 274
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 13, 19
CviJI RGCY 5 cut(s) 70, 131, 294, 326, 474
CviKI_1 RGCY 5 cut(s) 70, 131, 294, 326, 474
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 492
DpnI GATC 4 cut(s) 17, 135, 196, 503
DpnII GATC 4 cut(s) 15, 133, 194, 501
Eam1104I CTCTTC 2 cut(s) 60, 122
EarI CTCTTC 2 cut(s) 60, 122
Eco32I GATATC 1 cut(s) 460
EcoRV GATATC 1 cut(s) 460
FaeI CATG 2 cut(s) 16, 22
FaiI YATR 8 cut(s) 14, 20, 26, 112, 182, 453, 469, 471
FatI CATG 2 cut(s) 12, 18
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 50
FspBI CTAG 2 cut(s) 54, 514
HgaI GACGC 1 cut(s) 274
Hin1II CATG 2 cut(s) 16, 22
HincII GTYRAC 2 cut(s) 51, 172
HindII GTYRAC 2 cut(s) 51, 172
HinfI GANTC 2 cut(s) 229, 437
HphI GGTGA 3 cut(s) 266, 385, 472
Hpy166II GTNNAC 2 cut(s) 51, 172
Hpy188I TCNGA 4 cut(s) 194, 276, 399, 445
Hpy188III TCNNGA 2 cut(s) 125, 226
Hpy8I GTNNAC 2 cut(s) 51, 172
HpyAV CCTTC 1 cut(s) 154
HpyCH4III ACNGT 4 cut(s) 158, 188, 223, 509
HpyCH4IV ACGT 2 cut(s) 108, 497
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 2 cut(s) 108, 497
Hsp92II CATG 2 cut(s) 16, 22
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 4 cut(s) 15, 133, 194, 501
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 2 cut(s) 147, 276
MaeI CTAG 2 cut(s) 54, 514
MaeII ACGT 2 cut(s) 108, 497
MaeIII GTNAC 2 cut(s) 223, 286
MalI GATC 4 cut(s) 17, 135, 196, 503
MboI GATC 4 cut(s) 15, 133, 194, 501
MboII GAAGA 3 cut(s) 77, 109, 419
MflI RGATCY 1 cut(s) 194
MluCI AATT 3 cut(s) 308, 349, 368
MlyI GAGTC 1 cut(s) 238
MnlI CCTC 4 cut(s) 125, 244, 405, 493
MseI TTAA 3 cut(s) 75, 348, 386
MslI CAYNNNNRTG 2 cut(s) 17, 23
MwoI GCNNNNNNNGC 1 cut(s) 291
NdeII GATC 4 cut(s) 15, 133, 194, 501
NlaIII CATG 2 cut(s) 16, 22
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 2 cut(s) 223, 286
PciSI GCTCTTC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 437
Pfl23II CGTACG 1 cut(s) 363
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PspLI CGTACG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 325
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 2 cut(s) 17, 23
SalI GTCGAC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 75, 348, 386
Sau3AI GATC 4 cut(s) 15, 133, 194, 501
SchI GAGTC 1 cut(s) 238
SetI ASST 4 cut(s) 72, 111, 485, 500
SmiMI CAYNNNNRTG 2 cut(s) 17, 23
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 3 cut(s) 308, 349, 368
SspMI CTAG 2 cut(s) 54, 514
TaaI ACNGT 4 cut(s) 158, 188, 223, 509
TaiI ACGT 2 cut(s) 111, 500
TaqI TCGA 3 cut(s) 50, 153, 333
TasI AATT 3 cut(s) 308, 349, 368
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 3 cut(s) 75, 348, 386
Tru9I TTAA 3 cut(s) 75, 348, 386
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 2 cut(s) 223, 286
Tsp45I GTSAC 2 cut(s) 223, 286
TspDTI ATGAA 2 cut(s) 257, 429
TspRI CASTG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 2 cut(s) 54, 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.