AT4G00872

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013280
Physical Location & Seq
Reverse (-)
364027 .. 364757
731 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00872.1

Sequence Viewer

Length: 516 bp
ATGAAAACGATGATCAAGTTTCTGCTGTTAATACTGGTGATCTCTCCTATGTCGATATGCGCCGAGAAGGACATGATGATAAATGAATGCCACAATGCAGACGTCCCAGCCATATGCATGCAATGTCTTGAATCCGACCCAAACTCCATTCACGCCGACCATATAGGCATAGCTGGCATCGTCATAAACTGTCTTGAATCCCACCTCCATATCATCACCAATAACATAACGAATTTATCGTCAAAGAAAGATAAAGGTGAAGTGAAAACAGCTCTGGAGATTTGCAAAAAAGACTTGTCGACGAATGCATCTACAATACTGTCGGAAGCCAAAACAAGTTTGAAAACCGGTGATTATGATAAAACCAACAAATCGATAAAGCTTGCGCTTGGCTTTCCTCTGAGATGTATGTTCAATCTCAAACGTGTCAAGTTCACGTCTCCCCAACTATTTAGTCAAATCAATATCTATGCTCAACTCTCTGACGCTGCTATGAGGATCATTGATCGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

171

Amino Acids

19.03

Weight (kDa)

8.45

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 30 - 162 2.4e-09 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 299
AclWI GGATC 1 cut(s) 506
AcsI RAATTY 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 102
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 4 cut(s) 131, 197, 343, 415
AjiI CACGTC 1 cut(s) 438
AluBI AGCT 3 cut(s) 173, 272, 382
AluI AGCT 3 cut(s) 173, 272, 382
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 1 cut(s) 506
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 232
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AsiGI ACCGGT 1 cut(s) 347
AspLEI GCGC 2 cut(s) 62, 388
AsuHPI GGTGA 4 cut(s) 49, 208, 269, 362
BbvI GCAGC 1 cut(s) 475
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 444
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 438
BmsI GCATC 2 cut(s) 186, 317
BpmI CTGGAG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 102
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 39
Bse3DI GCAATG 1 cut(s) 128
BseCI ATCGAT 1 cut(s) 374
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 1 cut(s) 392
BseNI ACTGG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 475
BseYI CCCAGC 1 cut(s) 106
BshTI ACCGGT 1 cut(s) 347
BshVI ATCGAT 1 cut(s) 374
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 89
BsmAI GTCTC 1 cut(s) 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 2 cut(s) 92, 310
Bsp143I GATC 4 cut(s) 12, 39, 498, 505
BspCNI CTCAG 1 cut(s) 393
BspDI ATCGAT 1 cut(s) 374
BspPI GGATC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 39
BssAI RCCGGY 1 cut(s) 347
BssMI GATC 4 cut(s) 12, 39, 498, 505
BssNI GRCGYC 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 191, 321
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 119, 175, 384
BstDEI CTNAG 1 cut(s) 401
BstHHI GCGC 2 cut(s) 62, 388
BstKTI GATC 4 cut(s) 15, 42, 501, 508
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 4 cut(s) 12, 39, 498, 505
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNSI RCATGY 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 374
BsuTUI ATCGAT 1 cut(s) 374
BtrI CACGTC 1 cut(s) 438
Cac8I GCNNGC 3 cut(s) 119, 175, 384
CfoI GCGC 2 cut(s) 62, 388
Cfr10I RCCGGY 1 cut(s) 347
ClaI ATCGAT 1 cut(s) 374
CseI GACGC 1 cut(s) 494
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 2 cut(s) 73, 118
CviJI RGCY 6 cut(s) 110, 173, 272, 329, 382, 393
CviKI_1 RGCY 6 cut(s) 110, 173, 272, 329, 382, 393
DdeI CTNAG 1 cut(s) 401
DpnI GATC 4 cut(s) 14, 41, 500, 507
DpnII GATC 4 cut(s) 12, 39, 498, 505
EcoT22I ATGCAT 2 cut(s) 119, 310
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 2 cut(s) 76, 121
FaqI GGGAC 1 cut(s) 89
FatI CATG 2 cut(s) 72, 117
FauNDI CATATG 1 cut(s) 113
FbaI TGATCA 1 cut(s) 12
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 1 cut(s) 489
Fsp4HI GCNGC 1 cut(s) 489
GlaI GCGC 2 cut(s) 61, 387
GluI GCNGC 1 cut(s) 489
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 1 cut(s) 296
HapII CCGG 1 cut(s) 348
HgaI GACGC 1 cut(s) 494
HhaI GCGC 2 cut(s) 62, 388
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 76, 121
Hin6I GCGC 2 cut(s) 60, 386
HinP1I GCGC 2 cut(s) 60, 386
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 2 cut(s) 131, 197
HpaII CCGG 1 cut(s) 348
HphI GGTGA 4 cut(s) 49, 208, 269, 362
Hpy166II GTNNAC 2 cut(s) 300, 435
Hpy188I TCNGA 4 cut(s) 136, 325, 402, 484
Hpy188III TCNNGA 3 cut(s) 128, 194, 275
Hpy8I GTNNAC 2 cut(s) 300, 435
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 191, 321
HpyCH4IV ACGT 3 cut(s) 102, 424, 437
HpyCH4V TGCA 5 cut(s) 98, 117, 121, 285, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 3 cut(s) 102, 424, 437
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 76, 121
HspAI GCGC 2 cut(s) 60, 386
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 4 cut(s) 12, 39, 498, 505
LpnPI CCDG 5 cut(s) 20, 120, 159, 260, 361
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 2 cut(s) 186, 317
MaeII ACGT 3 cut(s) 102, 424, 437
MalI GATC 4 cut(s) 14, 41, 500, 507
MboI GATC 4 cut(s) 12, 39, 498, 505
MluCI AATT 1 cut(s) 232
MmeI TCCRAC 2 cut(s) 159, 303
MnlI CCTC 3 cut(s) 215, 408, 489
Mph1103I ATGCAT 2 cut(s) 119, 310
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 116
MspI CCGG 1 cut(s) 348
Mva1269I GAATGC 2 cut(s) 92, 310
MwoI GCNNNNNNNGC 1 cut(s) 174
NdeI CATATG 1 cut(s) 113
NdeII GATC 4 cut(s) 12, 39, 498, 505
NlaIII CATG 2 cut(s) 76, 121
NmeAIII GCCGAG 1 cut(s) 88
NsiI ATGCAT 2 cut(s) 119, 310
NspI RCATGY 1 cut(s) 121
PaeI GCATGC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 2 cut(s) 92, 310
PfeI GAWTC 2 cut(s) 131, 197
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 106
RseI CAYNNNNRTG 1 cut(s) 116
SalI GTCGAC 1 cut(s) 298
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 4 cut(s) 12, 39, 498, 505
SetI ASST 8 cut(s) 105, 175, 207, 259, 274, 384, 427, 440
SfaNI GCATC 2 cut(s) 186, 317
SmiMI CAYNNNNRTG 1 cut(s) 116
SphI GCATGC 1 cut(s) 121
Sse9I AATT 1 cut(s) 232
TaaI ACNGT 2 cut(s) 191, 321
TaiI ACGT 3 cut(s) 105, 427, 440
TaqI TCGA 3 cut(s) 53, 299, 374
TasI AATT 1 cut(s) 232
TfiI GAWTC 2 cut(s) 131, 197
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 17, 99
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 299
ZraI GACGTC 1 cut(s) 103
Zsp2I ATGCAT 2 cut(s) 119, 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.