AT3G16190

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006367
Physical Location & Seq
Reverse (-)
5489701 .. 5491332
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16190.1

Sequence Viewer

Length: 591 bp
ATGGCCGAGAGATGGAGGAACACGGCTCTACTCGTCATCGACATGCAGAACGATTTCATAGAGGAAGGTGCTGTGACGCAAGTGAAAGGAGGAAAATCTATAGTTCCTAATGTTATCAGAGTCGTCGAACTCGCGAGGCAGCGTGGTATTCTCGTAATTTGGGTTGTTCGAGAACATGATCGTCAAGGAAGAGATGTTGAATTATTCAGGCGCCATAACTACAGTTCTGAGAAAGTCGGGCCAGTTATTAAAGGCACCGTAGGAGCAGAATTGGTTGATGGATTGATGATCAACGAAGAAGATGACTATAAGATTGTGAAAACTCGTTTCAGTGCTTTCTTTAGTACCAATCTTCATTCCTTCTTGCAAACTTCAGGGGTTACCAAGTTAGTGATTGCTGGTGTGCAAACGCCGAACTGTATCCGGCAAACGGTGTTTGATGCAGTGGCGCTGGATTATCCCAATGTGACTGTTATTACAGATGCCACAGCTGCTGCAACACCAGAGATCCATACTGCGAATATTCTTGACATGAAGAATATTGGAGTCAAGACTCCTACATTACACGAGTGGTCCGAAGAACTTGCTTGA

Protein Analysis

196

Amino Acids

21.86

Weight (kDa)

5.75

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 8 - 185 1.5e-42 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 210, 254
AccII CGCG 1 cut(s) 134
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 357
AcyI GRCGYC 1 cut(s) 211
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 12, 430
AgsI TTSAA 1 cut(s) 200
AluBI AGCT 1 cut(s) 491
AluI AGCT 1 cut(s) 491
AlwI GGATC 1 cut(s) 502
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 2 cut(s) 3, 239
ApeKI GCWGC 3 cut(s) 139, 491, 494
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 213, 451
AspS9I GGNCC 2 cut(s) 239, 573
AvaII GGWCC 1 cut(s) 573
BanI GGYRCC 2 cut(s) 210, 254
BauI CACGAG 1 cut(s) 566
BbvI GCAGC 3 cut(s) 151, 478, 481
BccI CCATC 2 cut(s) 6, 272
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 1 cut(s) 566
BciVI GTATCC 1 cut(s) 431
BclI TGATCA 1 cut(s) 288
BfmI CTRYAG 2 cut(s) 99, 220
BfoI RGCGCY 2 cut(s) 214, 452
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 3 cut(s) 140, 492, 495
BlsI GCNGC 3 cut(s) 141, 493, 496
Bme18I GGWCC 1 cut(s) 573
BmgT120I GGNCC 2 cut(s) 239, 573
BmiI GGNNCC 2 cut(s) 212, 256
BmsI GCATC 2 cut(s) 430, 472
BsaHI GRCGYC 1 cut(s) 211
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 430
Bse1I ACTGG 1 cut(s) 242
BseLI CCNNNNNNNGG 2 cut(s) 12, 430
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 242
BseXI GCAGC 3 cut(s) 151, 478, 481
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 2 cut(s) 5, 241
BshNI GGYRCC 2 cut(s) 210, 254
BsiSI CCGG 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 12, 430
BsnI GGCC 2 cut(s) 5, 241
Bsp143I GATC 3 cut(s) 178, 288, 507
Bsp68I TCGCGA 1 cut(s) 134
BspANI GGCC 2 cut(s) 5, 241
BspCNI CTCAG 1 cut(s) 220
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 2 cut(s) 212, 256
BspPI GGATC 1 cut(s) 502
BspT107I GGYRCC 2 cut(s) 210, 254
BsrI ACTGG 1 cut(s) 242
BssMI GATC 3 cut(s) 178, 288, 507
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 566
Bst4CI ACNGT 5 cut(s) 224, 259, 419, 433, 472
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 379
BstFNI CGCG 1 cut(s) 134
BstH2I RGCGCY 2 cut(s) 214, 452
BstHHI GCGC 2 cut(s) 213, 451
BstKTI GATC 3 cut(s) 181, 291, 510
BstMBI GATC 3 cut(s) 178, 288, 507
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNSI RCATGY 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 379
BstSFI CTRYAG 2 cut(s) 99, 220
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 3 cut(s) 151, 478, 481
BstX2I RGATCY 1 cut(s) 507
BstYI RGATCY 1 cut(s) 507
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 2 cut(s) 5, 241
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 337, 450
BtuMI TCGCGA 1 cut(s) 134
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 2 cut(s) 213, 451
Cfr13I GGNCC 2 cut(s) 239, 573
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 345
CviAII CATG 3 cut(s) 43, 176, 532
CviJI RGCY 4 cut(s) 5, 26, 241, 491
CviKI_1 RGCY 4 cut(s) 5, 26, 241, 491
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 1 cut(s) 228
DinI GGCGCC 1 cut(s) 212
DpnI GATC 3 cut(s) 180, 290, 509
DpnII GATC 3 cut(s) 178, 288, 507
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 573
Eco57I CTGAAG 1 cut(s) 357
Eco91I GGTNACC 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 379
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
FaeI CATG 3 cut(s) 46, 179, 535
FaiI YATR 8 cut(s) 44, 59, 101, 177, 216, 309, 513, 533
FatI CATG 3 cut(s) 42, 175, 531
FbaI TGATCA 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 140, 492, 495
Fsp4HI GCNGC 3 cut(s) 140, 492, 495
GlaI GCGC 2 cut(s) 212, 450
GluI GCNGC 3 cut(s) 140, 492, 495
HaeII RGCGCY 2 cut(s) 214, 452
HaeIII GGCC 2 cut(s) 5, 241
HapII CCGG 1 cut(s) 424
HgaI GACGC 1 cut(s) 85
HhaI GCGC 2 cut(s) 213, 451
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 3 cut(s) 46, 179, 535
Hin6I GCGC 2 cut(s) 211, 449
HinP1I GCGC 2 cut(s) 211, 449
HinfI GANTC 3 cut(s) 120, 546, 553
HpaII CCGG 1 cut(s) 424
Hpy188I TCNGA 3 cut(s) 119, 229, 577
Hpy188III TCNNGA 4 cut(s) 133, 170, 527, 550
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 59, 370
HpyCH4III ACNGT 5 cut(s) 224, 259, 419, 433, 472
HpyCH4V TGCA 5 cut(s) 46, 367, 406, 443, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 3 cut(s) 46, 179, 535
HspAI GCGC 2 cut(s) 211, 449
KasI GGCGCC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 178, 288, 507
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 7 cut(s) 193, 255, 360, 384, 437, 437, 516
Lsp1109I GCAGC 3 cut(s) 151, 478, 481
LweI GCATC 2 cut(s) 430, 472
MaeIII GTNAC 3 cut(s) 73, 379, 466
MalI GATC 3 cut(s) 180, 290, 509
MboI GATC 3 cut(s) 178, 288, 507
MboII GAAGA 6 cut(s) 201, 308, 311, 344, 547, 590
MflI RGATCY 1 cut(s) 507
MluCI AATT 3 cut(s) 156, 200, 269
Mly113I GGCGCC 1 cut(s) 211
MlyI GAGTC 3 cut(s) 129, 547, 555
MnlI CCTC 4 cut(s) 9, 55, 83, 129
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 491
NarI GGCGCC 1 cut(s) 211
NdeII GATC 3 cut(s) 178, 288, 507
NlaIII CATG 3 cut(s) 46, 179, 535
NlaIV GGNNCC 2 cut(s) 212, 256
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 73, 466
NruI TCGCGA 1 cut(s) 134
NspI RCATGY 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PdmI GAANNNNTTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 141, 493, 496
PleI GAGTC 3 cut(s) 128, 547, 554
PluTI GGCGCC 1 cut(s) 214
PpsI GAGTC 3 cut(s) 128, 547, 554
PspEI GGTNACC 1 cut(s) 379
PspN4I GGNNCC 2 cut(s) 212, 256
PspPI GGNCC 2 cut(s) 239, 573
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 507
PvuII CAGCTG 1 cut(s) 491
RruI TCGCGA 1 cut(s) 134
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 3 cut(s) 140, 492, 495
Sau3AI GATC 3 cut(s) 178, 288, 507
Sau96I GGNCC 2 cut(s) 239, 573
SchI GAGTC 3 cut(s) 129, 547, 555
SetI ASST 2 cut(s) 70, 493
SfaNI GCATC 2 cut(s) 430, 472
SfcI CTRYAG 2 cut(s) 99, 220
SfoI GGCGCC 1 cut(s) 212
SinI GGWCC 1 cut(s) 573
SmiMI CAYNNNNRTG 1 cut(s) 41
Sse9I AATT 3 cut(s) 156, 200, 269
SspDI GGCGCC 1 cut(s) 210
SspI AATATT 2 cut(s) 523, 541
TaaI ACNGT 5 cut(s) 224, 259, 419, 433, 472
TaqI TCGA 3 cut(s) 39, 126, 169
TasI AATT 3 cut(s) 156, 200, 269
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 2 cut(s) 337, 450
TseFI GTSAC 2 cut(s) 73, 466
TseI GCWGC 3 cut(s) 139, 491, 494
Tsp45I GTSAC 2 cut(s) 73, 466
TspDTI ATGAA 3 cut(s) 46, 344, 548
TspRI CASTG 2 cut(s) 337, 450
VpaK11BI GGWCC 1 cut(s) 573
XceI RCATGY 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.