Rw2G033160

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006367
Physical Location & Seq
Reverse (-)
54334786 .. 54336894
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033160.1

Sequence Viewer

Length: 570 bp
ATGGGAGACAAATGGAAGCACACAGCTCTCCTCGTCGTTGACATGCAGAAAGATTTCATAGATGGTCCTGTGCTAGGGAAAGCAGGCAAAGCCATAGTCCCAAATGTAATAGAGGCAGTTCAAATCGCTAGGCAGCGTGGCATTCTCGTAGTTTGGGTTGTGCGTGAACATGATCCACTAGGAAGAGATGTTGAGCTATTTCGCCGGCATTTCTATTCTGCTGGGAAGGAGGGTCTAGCGGTTAAGGGTCAGATAGGTGCTGAACTTGTTGATGGGCTTGTGATTGAAGAAGGGGACTATAAATTAGTCAAGACCCGGTTTAGTGCATTCTTTGCTACACACCTTGATTCGTATCTCAAGACTAATGGAGTTGACAGTTTGGTTATCATTGGTGTTCAGACTCCAAACTGCATTAGGCAGACTGTGTTTGATGGCGTAGCTTTGGATTACAAATCTATTACAGTTATTGTTGATGCCACAGCTGCTGCTACAGCTGATGTACATGCCGCCAATATATTTGACATGAAGAATGTTGGAGTTGCAACACCAACCTTACAAGAATGGGTTTGA

Protein Analysis

189

Amino Acids

20.65

Weight (kDa)

5.93

Isoelectric Point (pI)

20.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 8 - 182 2.4e-37 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 239, 507
AclWI GGATC 1 cut(s) 167
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 1 cut(s) 74
AgsI TTSAA 2 cut(s) 122, 287
AluBI AGCT 5 cut(s) 26, 196, 440, 482, 494
AluI AGCT 5 cut(s) 26, 196, 440, 482, 494
AlwI GGATC 1 cut(s) 167
AlwNI CAGNNNCTG 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 133, 482, 485
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 65
AsuC2I CCSGG 1 cut(s) 316
AvaII GGWCC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 145, 469, 472
BccI CCATC 3 cut(s) 56, 266, 425
BcnI CCSGG 1 cut(s) 316
BfaI CTAG 4 cut(s) 74, 129, 179, 236
BfmI CTRYAG 1 cut(s) 489
BisI GCNGC 4 cut(s) 134, 483, 486, 507
BlsI GCNGC 4 cut(s) 135, 484, 487, 508
Bme1390I CCNGG 1 cut(s) 316
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 316
BmsI GCATC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 316
BsaBI GATNNNNATC 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse118I RCCGGY 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 351
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 145, 469, 472
BseYI CCCAGC 1 cut(s) 221
BsiSI CCGG 2 cut(s) 205, 316
BslFI GGGAC 2 cut(s) 83, 308
BslI CCNNNNNNNGG 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 83, 308
BsmI GAATGC 2 cut(s) 141, 326
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 2 cut(s) 239, 507
BspPI GGATC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 499
BssAI RCCGGY 1 cut(s) 204
BssMI GATC 1 cut(s) 172
Bst4CI ACNGT 3 cut(s) 377, 424, 463
Bst6I CTCTTC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 2 cut(s) 85, 206
BstENI CCTNNNNNAGG 1 cut(s) 72
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 4 cut(s) 89, 332, 482, 491
BstNSI RCATGY 2 cut(s) 46, 506
BstSCI CCNGG 1 cut(s) 314
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 3 cut(s) 145, 469, 472
Cac8I GCNNGC 2 cut(s) 85, 206
CaiI CAGNNNCTG 1 cut(s) 485
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 500
CviAII CATG 4 cut(s) 43, 170, 503, 523
CviJI RGCY 7 cut(s) 26, 92, 196, 277, 440, 482, 494
CviKI_1 RGCY 7 cut(s) 26, 92, 196, 277, 440, 482, 494
CviQI GTAC 1 cut(s) 500
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 65
EcoNI CCTNNNNNAGG 1 cut(s) 72
FaeI CATG 4 cut(s) 46, 173, 506, 526
FaiI YATR 8 cut(s) 44, 59, 95, 171, 300, 504, 515, 524
FaqI GGGAC 2 cut(s) 83, 308
FatI CATG 4 cut(s) 42, 169, 502, 522
Fnu4HI GCNGC 4 cut(s) 134, 483, 486, 507
Fsp4HI GCNGC 4 cut(s) 134, 483, 486, 507
FspBI CTAG 4 cut(s) 74, 129, 179, 236
GluI GCNGC 4 cut(s) 134, 483, 486, 507
GsaI CCCAGC 1 cut(s) 225
HapII CCGG 2 cut(s) 205, 316
Hin1II CATG 4 cut(s) 46, 173, 506, 526
HincII GTYRAC 2 cut(s) 40, 373
HindII GTYRAC 2 cut(s) 40, 373
HinfI GANTC 2 cut(s) 347, 400
HpaII CCGG 2 cut(s) 205, 316
Hpy166II GTNNAC 3 cut(s) 40, 167, 373
Hpy188I TCNGA 2 cut(s) 252, 399
Hpy188III TCNNGA 2 cut(s) 310, 358
Hpy8I GTNNAC 3 cut(s) 40, 167, 373
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 220, 284
HpyCH4III ACNGT 3 cut(s) 377, 424, 463
HpyCH4V TGCA 4 cut(s) 46, 326, 411, 542
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 332, 482, 491
Hsp92II CATG 4 cut(s) 46, 173, 506, 526
KroI GCCGGC 1 cut(s) 204
KroNI GCCGGC 1 cut(s) 206
Kzo9I GATC 1 cut(s) 172
LpnPI CCDG 5 cut(s) 69, 81, 207, 218, 329
Lsp1109I GCAGC 3 cut(s) 145, 469, 472
LweI GCATC 1 cut(s) 463
MaeI CTAG 4 cut(s) 74, 129, 179, 236
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 3 cut(s) 195, 299, 538
MluCI AATT 1 cut(s) 302
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 3 cut(s) 41, 106, 223
MroNI GCCGGC 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 1 cut(s) 243
MspA1I CMGCKG 2 cut(s) 482, 494
MspI CCGG 2 cut(s) 205, 316
MspR9I CCNGG 1 cut(s) 316
Mva1269I GAATGC 2 cut(s) 141, 326
MwoI GCNNNNNNNGC 4 cut(s) 89, 332, 482, 491
NaeI GCCGGC 1 cut(s) 206
NciI CCSGG 1 cut(s) 316
NdeII GATC 1 cut(s) 172
NgoMIV GCCGGC 1 cut(s) 204
NlaIII CATG 4 cut(s) 46, 173, 506, 526
NspI RCATGY 2 cut(s) 46, 506
PctI GAATGC 2 cut(s) 141, 326
PdiI GCCGGC 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 4 cut(s) 135, 484, 487, 508
PleI GAGTC 1 cut(s) 394
PpsI GAGTC 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 65
PstNI CAGNNNCTG 1 cut(s) 485
PvuII CAGCTG 2 cut(s) 482, 494
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 4 cut(s) 134, 483, 486, 507
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 1 cut(s) 65
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 316
SetI ASST 8 cut(s) 28, 198, 259, 345, 442, 484, 496, 554
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 302
SsiI CCGC 2 cut(s) 239, 507
SspMI CTAG 4 cut(s) 74, 129, 179, 236
StyD4I CCNGG 1 cut(s) 314
TaaI ACNGT 3 cut(s) 377, 424, 463
TasI AATT 1 cut(s) 302
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseI GCWGC 3 cut(s) 133, 482, 485
TspDTI ATGAA 2 cut(s) 46, 539
VpaK11BI GGWCC 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 72
XceI RCATGY 2 cut(s) 46, 506
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 4 cut(s) 74, 129, 179, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.