RLG00000019564

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006367
Physical Location & Seq
Forward (+)
53632756 .. 53634905
2150 bp
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UTR
Exon/CDS
Intron
RLM00000019564

Sequence Viewer

Length: 474 bp
ATGGGAGACAAATGGAAGCACACAGCTCTCCTCGTCATTGACATGCAGAAAGATTTTATAGATAGGGATGGTCCCATGCTAGTGAAAGGAGGCAAAGCCATGCTATTTCGCCGGCATTTGTATTCTGCTGGGAAAGAGGGTCCACCGGTTAAGGGTCAGATAGGTGCTGAACTTGTTGATGGGCTTACCTGGTTTAGTGCATTCTTTGCTACACACCTTGATTCCTATCTTAAGAGTAATGGAGTTGGAGTAATGGAGTTGACAGTTTGGTTGTCATTGGTAAGTGTTCAGACTCCAAACTGCATTAGGCAGACTGTGTTTCATGGTGTAGCTTTGGATTACAAATCTATTACAGTTATTGTTGATGCCACAGCTGCTGCTACTGCTGACGTACATGCTGCCAATATATTTGACACGAAGAACGTTGGAGTTGCAACACCAACCTTACAAGAATGGTCTGAATCGGGCACTTGA

Protein Analysis

158

Amino Acids

17.05

Weight (kDa)

6.17

Isoelectric Point (pI)

14.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 64 - 145 4.7e-09 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 423
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 152
AflII CTTAAG 1 cut(s) 230
AgeI ACCGGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 3 cut(s) 26, 332, 374
AluI AGCT 3 cut(s) 26, 332, 374
AlwNI CAGNNNCTG 1 cut(s) 377
ApeKI GCWGC 3 cut(s) 374, 377, 398
AsiGI ACCGGT 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 71, 140
AvaII GGWCC 2 cut(s) 71, 140
BaeGI GKGCMC 1 cut(s) 470
BbvI GCAGC 3 cut(s) 361, 364, 385
BccI CCATC 2 cut(s) 62, 173
BciT130I CCWGG 1 cut(s) 190
BfaI CTAG 1 cut(s) 80
BfrI CTTAAG 1 cut(s) 230
BisI GCNGC 3 cut(s) 375, 378, 399
BlsI GCNGC 3 cut(s) 376, 379, 400
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 2 cut(s) 71, 140
BmgT120I GGNCC 2 cut(s) 71, 140
BmiI GGNNCC 2 cut(s) 73, 141
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse118I RCCGGY 2 cut(s) 111, 145
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 73
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 152
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 470
BseXI GCAGC 3 cut(s) 361, 364, 385
BseYI CCCAGC 1 cut(s) 128
BshTI ACCGGT 1 cut(s) 145
BsiSI CCGG 2 cut(s) 112, 146
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 470
BspLI GGNNCC 2 cut(s) 73, 141
BspTI CTTAAG 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 111, 145
BssAI RCCGGY 2 cut(s) 111, 145
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 265, 316, 355
BstAFI CTTAAG 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 1 cut(s) 113
BstF5I GGATG 1 cut(s) 73
BstMWI GCNNNNNNNGC 3 cut(s) 206, 374, 383
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 2 cut(s) 46, 398
BstSCI CCNGG 1 cut(s) 188
BstSLI GKGCMC 1 cut(s) 470
BstV1I GCAGC 3 cut(s) 361, 364, 385
BtsCI GGATG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 113
CaiI CAGNNNCTG 1 cut(s) 377
Cfr10I RCCGGY 2 cut(s) 111, 145
Cfr13I GGNCC 2 cut(s) 71, 140
CsiI ACCWGGT 1 cut(s) 188
Csp6I GTAC 1 cut(s) 392
CspAI ACCGGT 1 cut(s) 145
CviAII CATG 5 cut(s) 43, 76, 100, 323, 395
CviJI RGCY 5 cut(s) 26, 98, 184, 332, 374
CviKI_1 RGCY 5 cut(s) 26, 98, 184, 332, 374
CviQI GTAC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 71, 140
EcoRII CCWGG 1 cut(s) 188
FaeI CATG 5 cut(s) 46, 79, 103, 326, 398
FaiI YATR 7 cut(s) 44, 59, 77, 101, 324, 396, 407
FaqI GGGAC 1 cut(s) 57
FatI CATG 5 cut(s) 42, 75, 99, 322, 394
Fnu4HI GCNGC 3 cut(s) 375, 378, 399
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 3 cut(s) 375, 378, 399
FspBI CTAG 1 cut(s) 80
GluI GCNGC 3 cut(s) 375, 378, 399
GsaI CCCAGC 1 cut(s) 132
HapII CCGG 2 cut(s) 112, 146
Hin1II CATG 5 cut(s) 46, 79, 103, 326, 398
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 3 cut(s) 221, 292, 461
HpaII CCGG 2 cut(s) 112, 146
Hpy166II GTNNAC 2 cut(s) 143, 261
Hpy188I TCNGA 3 cut(s) 159, 291, 460
Hpy8I GTNNAC 2 cut(s) 143, 261
HpyCH4III ACNGT 3 cut(s) 265, 316, 355
HpyCH4IV ACGT 2 cut(s) 390, 423
HpyCH4V TGCA 4 cut(s) 46, 200, 303, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 374, 383
HpySE526I ACGT 2 cut(s) 390, 423
Hsp92II CATG 5 cut(s) 46, 79, 103, 326, 398
KroI GCCGGC 1 cut(s) 111
KroNI GCCGGC 1 cut(s) 113
LpnPI CCDG 5 cut(s) 114, 125, 159, 175, 202
Lsp1109I GCAGC 3 cut(s) 361, 364, 385
LweI GCATC 1 cut(s) 355
MabI ACCWGGT 1 cut(s) 188
MaeI CTAG 1 cut(s) 80
MaeII ACGT 2 cut(s) 390, 423
MboII GAAGA 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 470
MlyI GAGTC 1 cut(s) 286
MmeI TCCRAC 2 cut(s) 226, 406
MnlI CCTC 3 cut(s) 41, 83, 130
MroNI GCCGGC 1 cut(s) 111
MseI TTAA 2 cut(s) 150, 231
MslI CAYNNNNRTG 2 cut(s) 41, 80
MspA1I CMGCKG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 2 cut(s) 112, 146
MspR9I CCNGG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 200
MvaI CCWGG 1 cut(s) 190
MwoI GCNNNNNNNGC 3 cut(s) 206, 374, 383
NaeI GCCGGC 1 cut(s) 113
NgoMIV GCCGGC 1 cut(s) 111
NlaIII CATG 5 cut(s) 46, 79, 103, 326, 398
NlaIV GGNNCC 2 cut(s) 73, 141
NspI RCATGY 2 cut(s) 46, 398
PctI GAATGC 1 cut(s) 200
PdiI GCCGGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 221, 461
PinAI ACCGGT 1 cut(s) 145
PkrI GCNGC 3 cut(s) 376, 379, 400
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 73, 141
PspPI GGNCC 2 cut(s) 71, 140
PstNI CAGNNNCTG 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
RseI CAYNNNNRTG 2 cut(s) 41, 80
SaqAI TTAA 2 cut(s) 150, 231
SatI GCNGC 3 cut(s) 375, 378, 399
Sau96I GGNCC 2 cut(s) 71, 140
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 470
SetI ASST 9 cut(s) 28, 166, 191, 219, 334, 376, 393, 426, 446
SexAI ACCWGGT 1 cut(s) 188
SfaNI GCATC 1 cut(s) 355
SinI GGWCC 2 cut(s) 71, 140
SmiMI CAYNNNNRTG 2 cut(s) 41, 80
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 265, 316, 355
TaiI ACGT 2 cut(s) 393, 426
TfiI GAWTC 2 cut(s) 221, 461
Tru1I TTAA 2 cut(s) 150, 231
Tru9I TTAA 2 cut(s) 150, 231
TseI GCWGC 3 cut(s) 374, 377, 398
TspDTI ATGAA 1 cut(s) 311
Vha464I CTTAAG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 71, 140
XceI RCATGY 2 cut(s) 46, 398
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.