Rh2BG416600

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006367
Physical Location & Seq
Reverse (-)
58783216 .. 58785979
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG416600.1

Sequence Viewer

Length: 615 bp
ATGGGAGACAGATGGAAGCACACAGCTCTCCTTGTCGTTGACATGCAGAAAGATTTTATAGATGGGGATGGTCCTATGCTAGTGAAAGGAGGCAAAGCCATAGTCCCAAATGTAATAGAGGCAGTTCAAATGGCTAGGCAGCGTGGCATTCTCGTAGTTTGGGTTGTGCGTGAACATGATCCATTAGGAAGAGATGTTGAGCTATTTCGCCGGCATTTGTATTCTGCTGGGAAAAAGGGTCCAACGGTTAAGGGTCAGATAGGTGCTGAACTTGTTGATGGGCTTGTGATTGAAGAAGGGGACTATAAATTAGTCAAGACCCGGTTTAGTGCATTCTTTGCTACACACCTTGATTCTTATCTTAAGAGTAATGGGGTTGACAGTTTGGTTGTCATTGGTGTTCAGACTCCAAACTGCATTAGGCAGACTGTGTTTGATGGCGTAGCTTTGGATTACAAATCTATTACAGTTATTGTTGATGCCACAGCTGCTGCTACAGCTGACGTACACGCTGGTATGTGCTTTCTGTATTTTGTGTTGGATTTCCAAAAACTTATGCTTCACCACTTCGGTAACCATCTCGATAGGATCTTCCCTTCAAAATTTGATAATTAA

Protein Analysis

204

Amino Acids

22.67

Weight (kDa)

6.7

Isoelectric Point (pI)

18.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 8 - 171 1.2e-35 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 173, 596
AcsI RAATTY 1 cut(s) 602
AfaI GTAC 1 cut(s) 507
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 128, 293, 600
AluBI AGCT 5 cut(s) 26, 202, 446, 488, 500
AluI AGCT 5 cut(s) 26, 202, 446, 488, 500
AlwI GGATC 2 cut(s) 173, 596
AlwNI CAGNNNCTG 1 cut(s) 491
ApeKI GCWGC 3 cut(s) 139, 488, 491
ApoI RAATTY 1 cut(s) 602
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspS9I GGNCC 2 cut(s) 71, 239
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 554
AvaII GGWCC 2 cut(s) 71, 239
BbvI GCAGC 3 cut(s) 151, 475, 478
BccI CCATC 6 cut(s) 6, 56, 62, 272, 431, 585
BcnI CCSGG 1 cut(s) 322
BfaI CTAG 2 cut(s) 80, 135
BfmI CTRYAG 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 362
BisI GCNGC 3 cut(s) 140, 489, 492
BlsI GCNGC 3 cut(s) 141, 490, 493
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 2 cut(s) 71, 239
BmgT120I GGNCC 2 cut(s) 71, 239
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 357
Bse118I RCCGGY 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 357
BseGI GGATG 1 cut(s) 73
BseJI GATNNNNATC 1 cut(s) 357
BseXI GCAGC 3 cut(s) 151, 475, 478
BseYI CCCAGC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 211, 322
BslFI GGGAC 2 cut(s) 89, 314
BsmFI GGGAC 2 cut(s) 89, 314
BsmI GAATGC 2 cut(s) 147, 332
Bsp143I GATC 2 cut(s) 178, 588
BspLI GGNNCC 1 cut(s) 240
BspPI GGATC 2 cut(s) 173, 596
BspTI CTTAAG 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 210
BssMI GATC 2 cut(s) 178, 588
Bst4CI ACNGT 4 cut(s) 247, 383, 430, 469
Bst6I CTCTTC 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 212
BstEII GGTNACC 1 cut(s) 572
BstF5I GGATG 1 cut(s) 73
BstKTI GATC 2 cut(s) 181, 591
BstMBI GATC 2 cut(s) 178, 588
BstMWI GCNNNNNNNGC 3 cut(s) 338, 488, 497
BstNSI RCATGY 1 cut(s) 46
BstPI GGTNACC 1 cut(s) 572
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 151, 475, 478
BstX2I RGATCY 1 cut(s) 588
BstYI RGATCY 1 cut(s) 588
BtsCI GGATG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 212
CaiI CAGNNNCTG 1 cut(s) 491
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 71, 239
Csp6I GTAC 1 cut(s) 506
CviAII CATG 2 cut(s) 43, 176
CviJI RGCY 8 cut(s) 26, 98, 134, 202, 283, 446, 488, 500
CviKI_1 RGCY 8 cut(s) 26, 98, 134, 202, 283, 446, 488, 500
CviQI GTAC 1 cut(s) 506
DpnI GATC 2 cut(s) 180, 590
DpnII GATC 2 cut(s) 178, 588
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 71, 239
Eco91I GGTNACC 1 cut(s) 572
EcoO65I GGTNACC 1 cut(s) 572
FaeI CATG 2 cut(s) 46, 179
FaiI YATR 8 cut(s) 44, 59, 77, 101, 177, 306, 518, 557
FaqI GGGAC 2 cut(s) 89, 314
FatI CATG 2 cut(s) 42, 175
Fnu4HI GCNGC 3 cut(s) 140, 489, 492
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 3 cut(s) 140, 489, 492
FspBI CTAG 2 cut(s) 80, 135
GluI GCNGC 3 cut(s) 140, 489, 492
GsaI CCCAGC 1 cut(s) 231
HapII CCGG 2 cut(s) 211, 322
Hin1II CATG 2 cut(s) 46, 179
HincII GTYRAC 2 cut(s) 40, 379
HindII GTYRAC 2 cut(s) 40, 379
HinfI GANTC 2 cut(s) 353, 406
HpaII CCGG 2 cut(s) 211, 322
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 4 cut(s) 40, 173, 379, 508
Hpy188I TCNGA 2 cut(s) 258, 405
Hpy188III TCNNGA 2 cut(s) 316, 581
Hpy8I GTNNAC 4 cut(s) 40, 173, 379, 508
HpyAV CCTTC 2 cut(s) 290, 606
HpyCH4III ACNGT 4 cut(s) 247, 383, 430, 469
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 3 cut(s) 46, 332, 417
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 488, 497
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 2 cut(s) 46, 179
KroI GCCGGC 1 cut(s) 210
KroNI GCCGGC 1 cut(s) 212
Kzo9I GATC 2 cut(s) 178, 588
LpnPI CCDG 4 cut(s) 213, 224, 335, 498
Lsp1109I GCAGC 3 cut(s) 151, 475, 478
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 80, 135
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 1 cut(s) 572
MalI GATC 2 cut(s) 180, 590
MboI GATC 2 cut(s) 178, 588
MboII GAAGA 3 cut(s) 201, 305, 583
MflI RGATCY 1 cut(s) 588
MluCI AATT 3 cut(s) 308, 602, 610
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 2 cut(s) 266, 519
MnlI CCTC 2 cut(s) 83, 112
MroNI GCCGGC 1 cut(s) 210
MseI TTAA 3 cut(s) 249, 363, 613
MspA1I CMGCKG 2 cut(s) 488, 500
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 2 cut(s) 211, 322
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 2 cut(s) 147, 332
MwoI GCNNNNNNNGC 3 cut(s) 338, 488, 497
NaeI GCCGGC 1 cut(s) 212
NciI CCSGG 1 cut(s) 322
NdeII GATC 2 cut(s) 178, 588
NgoMIV GCCGGC 1 cut(s) 210
NlaIII CATG 2 cut(s) 46, 179
NlaIV GGNNCC 1 cut(s) 240
NspI RCATGY 1 cut(s) 46
PctI GAATGC 2 cut(s) 147, 332
PdiI GCCGGC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 3 cut(s) 141, 490, 493
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 572
PspFI CCCAGC 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 2 cut(s) 71, 239
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 588
PvuII CAGCTG 2 cut(s) 488, 500
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SaqAI TTAA 3 cut(s) 249, 363, 613
SatI GCNGC 3 cut(s) 140, 489, 492
Sau3AI GATC 2 cut(s) 178, 588
Sau96I GGNCC 2 cut(s) 71, 239
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 322
SetI ASST 8 cut(s) 28, 204, 265, 351, 448, 490, 502, 507
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 2 cut(s) 71, 239
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 308, 602, 610
SspMI CTAG 2 cut(s) 80, 135
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 4 cut(s) 247, 383, 430, 469
TaiI ACGT 1 cut(s) 507
TaqI TCGA 1 cut(s) 582
TasI AATT 3 cut(s) 308, 602, 610
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 3 cut(s) 249, 363, 613
Tru9I TTAA 3 cut(s) 249, 363, 613
TseI GCWGC 3 cut(s) 139, 488, 491
Vha464I CTTAAG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 71, 239
XapI RAATTY 1 cut(s) 602
XceI RCATGY 1 cut(s) 46
XspI CTAG 2 cut(s) 80, 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.