RchiOBHm_Chr2g0139251

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006367
Physical Location & Seq
Reverse (-)
56862052 .. 56864880
2829 bp
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UTR
Exon/CDS
Intron
PRQ50983

Sequence Viewer

Length: 588 bp
ATGGGAGACAGATGGAAGCACACAGCTCTCCTTGTCGTTGACATGCAGAAAGATTTTATAGATGGGGATGGTCCTATGCTAGTGAAAGGAGGCAAAGCCATGGTCCCAAATGTAATAGAGGCAGTTCAAATGGCTAGGCAGCGTGGCATTCTCGTAGTTTGGGTTGTGCGTGAACATGATCCATTAGGAAGAGATGTTGAGCTATTTCGCCGGCATTTGTATTCTTCTGGGAAAAAGGGTCCAACGGTTAAGGGTCAGATAGGTGCTGAACTTGTTGATGGGCTTGTGATTGAAGAAGGGGACTATAAATTAGTCAAGACCCGGTTTAGTGCATTCTTTGCTACACACCTTGATTCTTATCTTAAGAGTAATGGGGTTGACAGTTTGGTTGTCATTGGTGTTCAGACTCCAAACTGCATTAGGCAGACTGTGTTTGATGGCGTAGCTTTGGATTACAAATCTATTACAGTTATTGTTGATGCCACAGCTGCTGCTACAGCTGACGTACACGCTGCCAATATATTTGACATGAAAAACATTGGAGTTGCAACACCAACCTTACAAGAATGGTCTGAATTGGGAGCTTGA

Protein Analysis

195

Amino Acids

21.28

Weight (kDa)

5.94

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isochorismatase PF00857 8 - 184 3.7e-38 Isochorismatase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 173
AfaI GTAC 1 cut(s) 507
AflII CTTAAG 1 cut(s) 362
AgsI TTSAA 2 cut(s) 128, 293
AluBI AGCT 6 cut(s) 26, 202, 446, 488, 500, 584
AluI AGCT 6 cut(s) 26, 202, 446, 488, 500, 584
AlwI GGATC 1 cut(s) 173
AlwNI CAGNNNCTG 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 139, 488, 491, 512
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspS9I GGNCC 3 cut(s) 71, 103, 239
AsuC2I CCSGG 1 cut(s) 322
AvaII GGWCC 3 cut(s) 71, 103, 239
BbvI GCAGC 4 cut(s) 151, 475, 478, 499
BccI CCATC 5 cut(s) 6, 56, 62, 272, 431
BcnI CCSGG 1 cut(s) 322
BfaI CTAG 2 cut(s) 80, 135
BfmI CTRYAG 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 362
BisI GCNGC 4 cut(s) 140, 489, 492, 513
BlsI GCNGC 4 cut(s) 141, 490, 493, 514
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 3 cut(s) 71, 103, 239
BmgT120I GGNCC 3 cut(s) 71, 103, 239
BmiI GGNNCC 2 cut(s) 105, 240
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 469
BpuMI CCSGG 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 73
BseJI GATNNNNATC 1 cut(s) 357
BseXI GCAGC 4 cut(s) 151, 475, 478, 499
BsiSI CCGG 2 cut(s) 211, 322
BslFI GGGAC 2 cut(s) 89, 314
BsmFI GGGAC 2 cut(s) 89, 314
BsmI GAATGC 2 cut(s) 147, 332
Bsp143I GATC 1 cut(s) 178
Bsp19I CCATGG 1 cut(s) 99
BspLI GGNNCC 2 cut(s) 105, 240
BspPI GGATC 1 cut(s) 173
BspTI CTTAAG 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 247, 383, 430, 469
Bst6I CTCTTC 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 212
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 73
BstKTI GATC 1 cut(s) 181
BstMBI GATC 1 cut(s) 178
BstMWI GCNNNNNNNGC 3 cut(s) 338, 488, 497
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 151, 475, 478, 499
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 212
CaiI CAGNNNCTG 1 cut(s) 491
Cfr10I RCCGGY 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 71, 103, 239
Csp6I GTAC 1 cut(s) 506
CviAII CATG 4 cut(s) 43, 100, 176, 529
CviJI RGCY 9 cut(s) 26, 98, 134, 202, 283, 446, 488, 500, 584
CviKI_1 RGCY 9 cut(s) 26, 98, 134, 202, 283, 446, 488, 500, 584
CviQI GTAC 1 cut(s) 506
DpnI GATC 1 cut(s) 180
DpnII GATC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 1 cut(s) 99
Eco47I GGWCC 3 cut(s) 71, 103, 239
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 4 cut(s) 46, 103, 179, 532
FaiI YATR 8 cut(s) 44, 59, 77, 101, 177, 306, 521, 530
FaqI GGGAC 2 cut(s) 89, 314
FatI CATG 4 cut(s) 42, 99, 175, 528
Fnu4HI GCNGC 4 cut(s) 140, 489, 492, 513
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 4 cut(s) 140, 489, 492, 513
FspBI CTAG 2 cut(s) 80, 135
GluI GCNGC 4 cut(s) 140, 489, 492, 513
HapII CCGG 2 cut(s) 211, 322
Hin1II CATG 4 cut(s) 46, 103, 179, 532
HincII GTYRAC 2 cut(s) 40, 379
HindII GTYRAC 2 cut(s) 40, 379
HinfI GANTC 2 cut(s) 353, 406
HpaII CCGG 2 cut(s) 211, 322
Hpy166II GTNNAC 4 cut(s) 40, 173, 379, 508
Hpy188I TCNGA 3 cut(s) 258, 405, 574
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 4 cut(s) 40, 173, 379, 508
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 4 cut(s) 247, 383, 430, 469
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 4 cut(s) 46, 332, 417, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 488, 497
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 4 cut(s) 46, 103, 179, 532
KroI GCCGGC 1 cut(s) 210
KroNI GCCGGC 1 cut(s) 212
Kzo9I GATC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 581
LpnPI CCDG 3 cut(s) 213, 224, 335
Lsp1109I GCAGC 4 cut(s) 151, 475, 478, 499
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 80, 135
MaeII ACGT 1 cut(s) 504
MalI GATC 1 cut(s) 180
MboI GATC 1 cut(s) 178
MboII GAAGA 3 cut(s) 201, 216, 305
MluCI AATT 2 cut(s) 308, 575
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 2 cut(s) 83, 112
MroNI GCCGGC 1 cut(s) 210
MseI TTAA 2 cut(s) 249, 363
MspA1I CMGCKG 2 cut(s) 488, 500
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 2 cut(s) 211, 322
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 2 cut(s) 147, 332
MwoI GCNNNNNNNGC 3 cut(s) 338, 488, 497
NaeI GCCGGC 1 cut(s) 212
NciI CCSGG 1 cut(s) 322
NcoI CCATGG 1 cut(s) 99
NdeII GATC 1 cut(s) 178
NgoMIV GCCGGC 1 cut(s) 210
NlaIII CATG 4 cut(s) 46, 103, 179, 532
NlaIV GGNNCC 2 cut(s) 105, 240
NspI RCATGY 1 cut(s) 46
PctI GAATGC 2 cut(s) 147, 332
PdiI GCCGGC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 353
PkrI GCNGC 4 cut(s) 141, 490, 493, 514
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 105, 240
PspPI GGNCC 3 cut(s) 71, 103, 239
PstNI CAGNNNCTG 1 cut(s) 491
PvuII CAGCTG 2 cut(s) 488, 500
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 249, 363
SatI GCNGC 4 cut(s) 140, 489, 492, 513
Sau3AI GATC 1 cut(s) 178
Sau96I GGNCC 3 cut(s) 71, 103, 239
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 322
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 3 cut(s) 71, 103, 239
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 2 cut(s) 308, 575
SspMI CTAG 2 cut(s) 80, 135
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 4 cut(s) 247, 383, 430, 469
TaiI ACGT 1 cut(s) 507
TasI AATT 2 cut(s) 308, 575
TfiI GAWTC 1 cut(s) 353
Tru1I TTAA 2 cut(s) 249, 363
Tru9I TTAA 2 cut(s) 249, 363
TseI GCWGC 4 cut(s) 139, 488, 491, 512
TspDTI ATGAA 1 cut(s) 545
Vha464I CTTAAG 1 cut(s) 362
VpaK11BI GGWCC 3 cut(s) 71, 103, 239
XceI RCATGY 1 cut(s) 46
XspI CTAG 2 cut(s) 80, 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.