Rorug02G0355900

Isochorismatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006367
Physical Location & Seq
Reverse (-)
44953544 .. 44957058
3515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0355900.1

Sequence Viewer

Length: 642 bp
ATGGGCCTCTCCATCTCAGCCCCTCTCTTCGACCATCGAGGCATCGACGGCCCTCGCTCTCCTCCTTTTGAGCCCTACAATGTCAGCAAAGTGGATCTTCTTGCCCAAAGTTGCTCTTTATGGCTCTTAATTTGGGGGTGCTCGGCACTTGGTGTCTGGAAGCTTAACACATTGGGGTTCCTTCTTACACATGCATCCAATTGGGTTTCATCCTTACCCCCTGCTCAGGTTAAGAGCAAGCTTAAAGGTCACTCTAAAAGGATAACTGGCCTAGCATTCTCTCATGTATTAAATGTGCTAGTTTCATCAGGAGCAGATGCTCAGGTTTGTGTGTGGAATTCTGATGGATGGGAAAAACAGAAGAGCAGATTCTTGCAGCTTCCAGCTGGGAGAACACCATCTTCAGTGTCAGATACTTGCGTTCAGTTTCATCAGGATCAAGCACATTTCTTGGTTGTGCATGAGACTCAGCTGGCCATATTTGAAACAACAAAACTAGAATGTGTAAAGCAGTGGCTCCCATGTGAGTCTGCTGCAGAAAAACAAGAAGGGGCAAGAGACCTAGAAAGGGAGGCAACCGATTCTGGAAGAGTATTGGGCTGCGCACCAAGCCCCACAGGGATGCCTCTTGGGAAAATCTAA

Protein Analysis

213

Amino Acids

23.23

Weight (kDa)

7.64

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 75 - 171 1.7e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 76 - 171 2.8e-06 WDR5 beta-propeller domain
WD40 PF00400 78 - 113 5.1e-06 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 78 - 113 8.1e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 79 - 168 5.3e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 604
AclWI GGATC 2 cut(s) 102, 444
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 387
AdeI CACNNNGTG 1 cut(s) 152
AfiI CCNNNNNNNGG 2 cut(s) 226, 568
AgsI TTSAA 1 cut(s) 485
AluBI AGCT 5 cut(s) 163, 241, 379, 386, 472
AluI AGCT 5 cut(s) 163, 241, 379, 386, 472
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 2 cut(s) 458, 552
AlwI GGATC 2 cut(s) 102, 444
AoxI GGCC 4 cut(s) 4, 49, 268, 474
ApeKI GCWGC 3 cut(s) 376, 533, 600
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 1 cut(s) 605
AspS9I GGNCC 2 cut(s) 4, 50
BaeI ACNNNNGTAYC 2 cut(s) 405, 438
BalI TGGCCA 1 cut(s) 476
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 3 cut(s) 388, 520, 587
BccI CCATC 5 cut(s) 20, 42, 338, 342, 406
BceAI ACGGC 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 458, 552
BfaI CTAG 4 cut(s) 272, 299, 497, 563
BfmI CTRYAG 1 cut(s) 534
BisI GCNGC 3 cut(s) 377, 534, 601
BlsI GCNGC 3 cut(s) 378, 535, 602
BmgT120I GGNCC 2 cut(s) 4, 50
BmiI GGNNCC 2 cut(s) 179, 518
BmsI GCATC 4 cut(s) 51, 203, 307, 612
Bpu10I CCTNAGC 2 cut(s) 225, 321
BsaI GGTCTC 1 cut(s) 552
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 568
Bse1I ACTGG 1 cut(s) 271
BseGI GGATG 4 cut(s) 194, 209, 353, 627
BseLI CCNNNNNNNGG 2 cut(s) 226, 568
BseMII CTCAG 4 cut(s) 30, 239, 335, 482
BseNI ACTGG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 388, 520, 587
BseYI CCCAGC 1 cut(s) 386
BshFI GGCC 4 cut(s) 6, 51, 270, 476
BsiHKAI GWGCWC 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 226, 568
BsmAI GTCTC 2 cut(s) 458, 552
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 4 cut(s) 6, 51, 270, 476
Bso31I GGTCTC 1 cut(s) 552
Bsp1286I GDGCHC 2 cut(s) 75, 143
Bsp143I GATC 2 cut(s) 94, 436
BspANI GGCC 4 cut(s) 6, 51, 270, 476
BspCNI CTCAG 4 cut(s) 29, 238, 334, 481
BspLI GGNNCC 2 cut(s) 179, 518
BspMAI CTGCAG 1 cut(s) 538
BspPI GGATC 2 cut(s) 102, 444
BspQI GCTCTTC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 552
BsrI ACTGG 1 cut(s) 271
BssMI GATC 2 cut(s) 94, 436
Bst6I CTCTTC 3 cut(s) 32, 356, 583
BstC8I GCNNGC 2 cut(s) 239, 474
BstDEI CTNAG 4 cut(s) 16, 225, 321, 468
BstF5I GGATG 4 cut(s) 194, 209, 353, 627
BstHHI GCGC 1 cut(s) 605
BstKTI GATC 2 cut(s) 97, 439
BstMAI GTCTC 2 cut(s) 458, 552
BstMBI GATC 2 cut(s) 94, 436
BstMWI GCNNNNNNNGC 2 cut(s) 48, 609
BstNSI RCATGY 1 cut(s) 194
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 3 cut(s) 388, 520, 587
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 4 cut(s) 6, 51, 270, 476
BtsCI GGATG 4 cut(s) 194, 209, 353, 627
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 2 cut(s) 411, 518
Cac8I GCNNGC 2 cut(s) 239, 474
CfoI GCGC 1 cut(s) 605
Cfr13I GGNCC 2 cut(s) 4, 50
CviAII CATG 4 cut(s) 191, 284, 461, 522
DdeI CTNAG 4 cut(s) 16, 225, 321, 468
DpnI GATC 2 cut(s) 96, 438
DpnII GATC 2 cut(s) 94, 436
DraIII CACNNNGTG 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 3 cut(s) 32, 356, 583
EarI CTCTTC 3 cut(s) 32, 356, 583
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 552
Eco57I CTGAAG 1 cut(s) 387
EcoRI GAATTC 1 cut(s) 337
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 75
FaeI CATG 4 cut(s) 194, 287, 464, 525
FaiI YATR 6 cut(s) 121, 192, 285, 462, 479, 523
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 100, 132
FatI CATG 4 cut(s) 190, 283, 460, 521
Fnu4HI GCNGC 3 cut(s) 377, 534, 601
FokI GGATG 4 cut(s) 181, 196, 360, 634
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 377, 534, 601
FspBI CTAG 4 cut(s) 272, 299, 497, 563
FspI TGCGCA 1 cut(s) 604
GlaI GCGC 1 cut(s) 604
GluI GCNGC 3 cut(s) 377, 534, 601
GsaI CCCAGC 1 cut(s) 390
HaeIII GGCC 4 cut(s) 6, 51, 270, 476
HhaI GCGC 1 cut(s) 605
Hin1II CATG 4 cut(s) 194, 287, 464, 525
Hin6I GCGC 1 cut(s) 603
HinP1I GCGC 1 cut(s) 603
HindIII AAGCTT 2 cut(s) 161, 239
HinfI GANTC 4 cut(s) 369, 466, 527, 581
Hpy188I TCNGA 2 cut(s) 343, 412
Hpy188III TCNNGA 4 cut(s) 157, 309, 434, 585
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 191, 542
HpyCH4V TGCA 4 cut(s) 194, 376, 460, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 609
HpyF3I CTNAG 4 cut(s) 16, 225, 321, 468
Hsp92II CATG 4 cut(s) 194, 287, 464, 525
HspAI GCGC 1 cut(s) 603
Kzo9I GATC 2 cut(s) 94, 436
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 2 cut(s) 311, 522
Lsp1109I GCAGC 3 cut(s) 388, 520, 587
LweI GCATC 4 cut(s) 51, 203, 307, 612
MaeI CTAG 4 cut(s) 272, 299, 497, 563
MaeIII GTNAC 1 cut(s) 248
MalI GATC 2 cut(s) 96, 438
MboI GATC 2 cut(s) 94, 436
MboII GAAGA 5 cut(s) 19, 89, 373, 393, 600
MfeI CAATTG 1 cut(s) 199
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 2 cut(s) 75, 143
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 3 cut(s) 129, 199, 337
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 2 cut(s) 460, 536
MnlI CCTC 7 cut(s) 17, 32, 33, 63, 72, 565, 636
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 1 cut(s) 196
MscI TGGCCA 1 cut(s) 476
MseI TTAA 5 cut(s) 128, 165, 231, 243, 290
MslI CAYNNNNRTG 1 cut(s) 620
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 386, 472
MunI CAATTG 1 cut(s) 199
Mva1269I GAATGC 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 48, 609
NdeII GATC 2 cut(s) 94, 436
NlaIII CATG 4 cut(s) 194, 287, 464, 525
NlaIV GGNNCC 2 cut(s) 179, 518
NmeAIII GCCGAG 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 248
NsbI TGCGCA 1 cut(s) 604
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 356
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 2 cut(s) 369, 581
PkrI GCNGC 3 cut(s) 378, 535, 602
PleI GAGTC 2 cut(s) 460, 535
PpsI GAGTC 2 cut(s) 460, 535
PspFI CCCAGC 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 179, 518
PspPI GGNCC 2 cut(s) 4, 50
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 94
PvuII CAGCTG 2 cut(s) 386, 472
RseI CAYNNNNRTG 1 cut(s) 620
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 5 cut(s) 128, 165, 231, 243, 290
SatI GCNGC 3 cut(s) 377, 534, 601
Sau3AI GATC 2 cut(s) 94, 436
Sau96I GGNCC 2 cut(s) 4, 50
SchI GAGTC 2 cut(s) 460, 536
SduI GDGCHC 2 cut(s) 75, 143
SetI ASST 9 cut(s) 165, 231, 243, 250, 327, 381, 388, 474, 564
SfaNI GCATC 4 cut(s) 51, 203, 307, 612
SfcI CTRYAG 1 cut(s) 534
SmiMI CAYNNNNRTG 1 cut(s) 620
Sse9I AATT 3 cut(s) 129, 199, 337
SspMI CTAG 4 cut(s) 272, 299, 497, 563
TaqI TCGA 3 cut(s) 30, 37, 45
TasI AATT 3 cut(s) 129, 199, 337
TfiI GAWTC 2 cut(s) 369, 581
Tru1I TTAA 5 cut(s) 128, 165, 231, 243, 290
Tru9I TTAA 5 cut(s) 128, 165, 231, 243, 290
TscAI CASTG 2 cut(s) 411, 518
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 3 cut(s) 376, 533, 600
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 198, 294, 419
TspRI CASTG 2 cut(s) 411, 518
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 194
XspI CTAG 4 cut(s) 272, 299, 497, 563
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.