AT3G16500

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010805
Physical Location & Seq
Reverse (-)
5612203 .. 5614803
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16500.1

Sequence Viewer

Length: 810 bp
ATGGAAGGTTGTCCAAGAAACAGAGAAATCGGTCCAAAACTTCTTGATTTGATTCCACAAGGAAGGAAATGGTACCAAGAAGACAAGAACAACACAGATCAGGAGAAGAAACTTGAGCTAAGGCTTGGACCACCCGGTGGTGATGAAGAAGATCATTCAGCAATCAAGAAGAAGAACACAGAGATAAGAAACATCAAGAAGGAAACAGAAGACAAATCTTTCCACTGTTTTAATGGCAATCACTTCTCTCCTTCCAACAAAACCACTTCTGTTCCTCACATCTCACAGAAAAGAACTGCTCCTGGTCCAGTGGTGGGTTGGCCTCCGGTTCGTTCGTTCAGAAAGAATCTAGCGAGCACAAGCTCTTCAAAGCTAGGTAACGAATCCTCTCATGGAGGTCAAATCAACAAGAGTGATGATGGTGAAAAACAAGTTGAAACCAAGAAGGAAGGAATGTTTGTCAAGATCAACATGGATGGTGTTCCTATTGGTCGTAAAGTTGATCTCAATGCTTACAACAGCTACGAACAGCTCTCTTTTGTCGTTGACAAACTCTTCAGAGGTCTACTCGCAGCTCAAAGAGATATCTCTGATGGTCAAGGAGAAGAGAAACCTATCATTGGATTATTAGATGGGAAAGGAGAATTTACTTTAACCTATGAAGACAATGAAGGGGACAAGATGCTTGTTGGGGATGTTCCATGGCAAATGTTTGTTTCATCTGTGAAGAGACTGCGTGTGATTAAAAGCTCTGAGATTTCATCTGCCTTGACATTTGGATGCAGTAAGCAAGAGAAGATGATGCACTGA

Protein Analysis

269

Amino Acids

30.12

Weight (kDa)

8.58

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 38 - 254 4.2e-64 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51950 AT3G16500 AT3G16500
fragaria_vesca FvH4_6g32450 FvH4_6g32450
malus_domestica MD09G1202300.v1.1 MD17G1183500.v1.1
prunus_persica Prupe.3G058600_v2.0.a1
pyrus_communis pycom09g11930 pycom17g19260
rosa_chinensis RchiOBHm_Chr2g0140681
rosa_laevigata RLG00000019891
rosa_multiflora Rmu_sc0003342.1_g000011
rosa_roxburghii Rroxscaffold_2G00104590
rosa_rugosa Rorug02G0364000
rosa_samantha Rh2AG415700 Rh2BG425200 Rh2CG401700 Rh2DG435200
rosa_wichuraiana Rw2G034010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 137
AccI GTMKAC 1 cut(s) 565
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 1 cut(s) 541
AdeI CACNNNGTG 1 cut(s) 137
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 3 cut(s) 137, 314, 620
AgsI TTSAA 2 cut(s) 369, 437
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 7 cut(s) 118, 363, 373, 522, 532, 575, 750
AluI AGCT 7 cut(s) 118, 363, 373, 522, 532, 575, 750
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 724
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 1 cut(s) 644
Asp718I GGTACC 1 cut(s) 72
AspS9I GGNCC 3 cut(s) 32, 128, 305
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 152, 434
AvaII GGWCC 3 cut(s) 32, 128, 305
BanI GGYRCC 1 cut(s) 72
BbsI GAAGAC 3 cut(s) 87, 216, 669
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 584
BccI CCATC 4 cut(s) 413, 470, 587, 626
BciT130I CCWGG 1 cut(s) 303
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 724
BfaI CTAG 2 cut(s) 350, 374
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 2 cut(s) 135, 303
Bme18I GGWCC 3 cut(s) 32, 128, 305
BmgT120I GGNCC 3 cut(s) 32, 128, 305
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 135, 303
BmsI GCATC 3 cut(s) 672, 770, 792
BpiI GAAGAC 3 cut(s) 87, 216, 669
BplI GAGNNNNNCTC 2 cut(s) 552, 584
Bpu10I CCTNAGC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 314, 620
Bse1I ACTGG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 3 cut(s) 481, 700, 785
BseLI CCNNNNNNNGG 3 cut(s) 137, 314, 620
BseMII CTCAG 1 cut(s) 744
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 584
BshFI GGCC 1 cut(s) 322
BshNI GGYRCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 135, 326
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 3 cut(s) 137, 314, 620
BsmAI GTCTC 1 cut(s) 724
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 4 cut(s) 97, 151, 465, 502
Bsp19I CCATGG 1 cut(s) 701
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 72
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 701
BssMI GATC 4 cut(s) 97, 151, 465, 502
BssT1I CCWWGG 1 cut(s) 701
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 4 cut(s) 370, 560, 600, 722
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 119, 753
BstDSI CCRYGG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 481, 700, 785
BstKTI GATC 4 cut(s) 100, 154, 468, 505
BstMAI GTCTC 1 cut(s) 724
BstMBI GATC 4 cut(s) 97, 151, 465, 502
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 133, 301
BstV1I GCAGC 1 cut(s) 584
BstV2I GAAGAC 3 cut(s) 87, 216, 669
BsuRI GGCC 1 cut(s) 322
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 3 cut(s) 481, 700, 785
BtsIMutI CAGTG 3 cut(s) 223, 315, 805
Cac8I GCNNGC 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 32, 128, 305
Csp6I GTAC 1 cut(s) 73
CviAII CATG 3 cut(s) 392, 472, 702
CviJI RGCY 9 cut(s) 118, 124, 322, 363, 373, 522, 532, 575, 750
CviKI_1 RGCY 9 cut(s) 118, 124, 322, 363, 373, 522, 532, 575, 750
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 2 cut(s) 119, 753
DpnI GATC 4 cut(s) 99, 153, 467, 504
DpnII GATC 4 cut(s) 97, 151, 465, 502
DraIII CACNNNGTG 1 cut(s) 137
Eam1104I CTCTTC 4 cut(s) 370, 560, 600, 722
EarI CTCTTC 4 cut(s) 370, 560, 600, 722
Eco130I CCWWGG 1 cut(s) 701
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 3 cut(s) 32, 128, 305
Eco57I CTGAAG 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 301
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 701
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 3 cut(s) 395, 475, 705
FaiI YATR 4 cut(s) 393, 473, 660, 703
FaqI GGGAC 1 cut(s) 689
FatI CATG 3 cut(s) 391, 471, 701
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 3 cut(s) 488, 707, 792
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 2 cut(s) 350, 374
GluI GCNGC 1 cut(s) 573
HaeIII GGCC 1 cut(s) 322
HapII CCGG 2 cut(s) 135, 326
Hin1II CATG 3 cut(s) 395, 475, 705
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 3 cut(s) 52, 346, 383
HpaII CCGG 2 cut(s) 135, 326
HphI GGTGA 2 cut(s) 152, 434
Hpy166II GTNNAC 2 cut(s) 547, 566
Hpy188I TCNGA 4 cut(s) 341, 560, 592, 754
Hpy188III TCNNGA 5 cut(s) 44, 101, 166, 196, 463
Hpy8I GTNNAC 2 cut(s) 547, 566
HpyAV CCTTC 6 cut(s) 57, 193, 261, 439, 443, 665
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4V TGCA 2 cut(s) 783, 805
HpyF3I CTNAG 2 cut(s) 119, 753
Hsp92II CATG 3 cut(s) 395, 475, 705
KpnI GGTACC 1 cut(s) 76
Kzo9I GATC 4 cut(s) 97, 151, 465, 502
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 6 cut(s) 86, 148, 288, 315, 321, 339
Lsp1109I GCAGC 1 cut(s) 584
LweI GCATC 3 cut(s) 672, 770, 792
MaeI CTAG 2 cut(s) 350, 374
MaeIII GTNAC 1 cut(s) 377
MalI GATC 4 cut(s) 99, 153, 467, 504
MboI GATC 4 cut(s) 97, 151, 465, 502
MhlI GDGCHC 1 cut(s) 359
MluCI AATT 1 cut(s) 644
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 5 cut(s) 285, 333, 389, 397, 554
MseI TTAA 3 cut(s) 231, 653, 744
MslI CAYNNNNRTG 1 cut(s) 778
MspI CCGG 2 cut(s) 135, 326
MspR9I CCNGG 2 cut(s) 135, 303
MvaI CCWGG 1 cut(s) 303
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 701
NdeII GATC 4 cut(s) 97, 151, 465, 502
NlaIII CATG 3 cut(s) 395, 475, 705
NlaIV GGNNCC 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 370
PfeI GAWTC 3 cut(s) 52, 346, 383
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 3 cut(s) 32, 128, 305
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 778
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 3 cut(s) 231, 653, 744
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 4 cut(s) 97, 151, 465, 502
Sau96I GGNCC 3 cut(s) 32, 128, 305
ScrFI CCNGG 2 cut(s) 135, 303
SduI GDGCHC 1 cut(s) 359
SfaNI GCATC 3 cut(s) 672, 770, 792
SinI GGWCC 3 cut(s) 32, 128, 305
SmiMI CAYNNNNRTG 1 cut(s) 778
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 1 cut(s) 644
SspMI CTAG 2 cut(s) 350, 374
StyD4I CCNGG 2 cut(s) 133, 301
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 1 cut(s) 227
TaqII GACCGA 1 cut(s) 20
TasI AATT 1 cut(s) 644
TfiI GAWTC 3 cut(s) 52, 346, 383
Tru1I TTAA 3 cut(s) 231, 653, 744
Tru9I TTAA 3 cut(s) 231, 653, 744
TscAI CASTG 2 cut(s) 230, 315
TseI GCWGC 1 cut(s) 572
TspDTI ATGAA 5 cut(s) 159, 675, 684, 708, 750
TspRI CASTG 2 cut(s) 230, 315
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 32, 128, 305
XapI RAATTY 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 2 cut(s) 230, 315
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 2 cut(s) 350, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.