Rh2DG435200

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0010805
Physical Location & Seq
Forward (+)
62974685 .. 62979753
5069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG435200.1

Sequence Viewer

Length: 912 bp
ATGCAAAAGATGAAGGAGGCATGTCCTCAACTTCTGGATTTGATTCCCAAAGAAAGAGAGTGGCTTGTGAGCAAAGATGAGAGCCATGGCTCTTCAGAAGAGAAGAAATTGGAGCTAAGGCTTGGTCCTCCAGGTCAAGACTGGTCAAATCTGAGAGACAACACCTCCAGAGGCCACAAAGAAAGAGAAGAGTCTCTTCTTTCTCTTGGGTACTTCTACAACAGCAGCAGCAATGGAAACCAAACCCACCATTTTGCTTCCTCAGAGAGAAAGACTGTTCTGCCCTCTCCATGGTCTTCTTCTCCCTCAGGGTACCACCACAACCAGACAAAAGGTCCTTCCTTTCTTCAGTTCTCACCAAGCTCTCAGAGCCTGCCTGTCACCCAGAAGGCGTCACATCCCTGTTGCACTAAAGCGGTAGACTTGCAGAGCGCAGAGAAGAAGAAAGCTTCTGCAAATCCAGCTGTGACCAACAACACTTCTCAGAAAAGAACTGCTCCTGCTCCAGTCGTGGGTTGGCCTCCAATTCGATCTTTTCGGAAGAATCTTGCGAGCACCAGCTCTTCAAAACCAGCACCTGAGTCACAAAATGTAGCCCCTAGCAAGGTTCCTAATGTTAAACCAGTTGAAACCAGCGGAAAAGGCATGTTTGTGAAAATCAATATGGATGGAGTCCCCATTGGACGAAAAGTAGACCTCAGTGCTTATGCCAGCTACGAAAAGCTATCATGTGCTGTTGATGAACTCTTCCGTGGGCTCCTTGCAGGTAATGATCATAGTTCTATAGTTACTGTAGTGACTTTACCTATTGCAAATTGCTCCTGTTCTTTTTGGTTAGTAAAGAGAATTATTCAATTCATAGGTCATGTTGGTTATATTCAACTTCTAGTTCATGTTTCAACTATTAATTGA

Protein Analysis

303

Amino Acids

33.21

Weight (kDa)

9.38

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 37 - 259 4.3e-37 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51950 AT3G16500 AT3G16500
fragaria_vesca FvH4_6g32450 FvH4_6g32450
malus_domestica MD09G1202300.v1.1 MD17G1183500.v1.1
prunus_persica Prupe.3G058600_v2.0.a1
pyrus_communis pycom09g11930 pycom17g19260
rosa_chinensis RchiOBHm_Chr2g0140681
rosa_laevigata RLG00000019891
rosa_multiflora Rmu_sc0003342.1_g000011
rosa_roxburghii Rroxscaffold_2G00104590
rosa_rugosa Rorug02G0364000
rosa_samantha Rh2AG415700 Rh2BG425200 Rh2CG401700 Rh2DG435200
rosa_wichuraiana Rw2G034010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 755
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 420, 693
AciI CCGC 2 cut(s) 416, 636
AcuI CTGAAG 2 cut(s) 78, 332
AcyI GRCGYC 1 cut(s) 392
AfaI GTAC 2 cut(s) 212, 314
AfiI CCNNNNNNNGG 3 cut(s) 291, 512, 604
AgsI TTSAA 5 cut(s) 567, 629, 854, 881, 900
AhdI GACNNNNNGTC 1 cut(s) 333
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 7 cut(s) 115, 363, 449, 464, 561, 714, 724
AluI AGCT 7 cut(s) 115, 363, 449, 464, 561, 714, 724
Alw21I GWGCWC 1 cut(s) 557
Alw26I GTCTC 2 cut(s) 150, 198
AlwNI CAGNNNCTG 2 cut(s) 373, 578
AoxI GGCC 2 cut(s) 172, 518
ApeKI GCWGC 2 cut(s) 225, 228
AseI ATTAAT 1 cut(s) 906
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 125, 335
AsuHPI GGTGA 2 cut(s) 348, 373
AvaII GGWCC 2 cut(s) 125, 335
AxyI CCTNAGG 1 cut(s) 307
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 759
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 557
BbvI GCAGC 2 cut(s) 237, 240
BccI CCATC 1 cut(s) 662
BciT130I CCWGG 1 cut(s) 132
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 2 cut(s) 150, 198
BfaI CTAG 2 cut(s) 600, 887
BfmI CTRYAG 2 cut(s) 783, 792
BfuAI ACCTGC 1 cut(s) 755
BisI GCNGC 2 cut(s) 226, 229
BlsI GCNGC 2 cut(s) 227, 230
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 125, 335
BmeRI GACNNNNNGTC 1 cut(s) 333
BmgT120I GGNCC 2 cut(s) 125, 335
BmiI GGNNCC 3 cut(s) 314, 609, 758
BmrFI CCNGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 3 cut(s) 114, 151, 489
Bpu10I CCTNAGC 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 392
BsaJI CCNNGG 3 cut(s) 85, 290, 751
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 512, 604
Bse1I ACTGG 3 cut(s) 146, 506, 623
Bse21I CCTNAGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 3 cut(s) 85, 290, 751
BseGI GGATG 2 cut(s) 397, 673
BseLI CCNNNNNNNGG 3 cut(s) 291, 512, 604
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 7 cut(s) 143, 276, 321, 380, 497, 570, 712
BseNI ACTGG 3 cut(s) 146, 506, 623
BseXI GCAGC 2 cut(s) 237, 240
BshFI GGCC 2 cut(s) 174, 520
BshNI GGYRCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 557
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 3 cut(s) 291, 512, 604
BsmAI GTCTC 2 cut(s) 150, 198
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 2 cut(s) 174, 520
Bsp1286I GDGCHC 2 cut(s) 557, 759
Bsp143I GATC 2 cut(s) 530, 772
Bsp19I CCATGG 2 cut(s) 85, 290
BspACI CCGC 2 cut(s) 416, 636
BspANI GGCC 2 cut(s) 174, 520
BspCNI CTCAG 7 cut(s) 144, 275, 320, 379, 496, 571, 711
BspLI GGNNCC 3 cut(s) 314, 609, 758
BspMI ACCTGC 1 cut(s) 755
BspQI GCTCTTC 2 cut(s) 97, 568
BspT107I GGYRCC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 238
BsrI ACTGG 3 cut(s) 146, 506, 623
BssECI CCNNGG 3 cut(s) 85, 290, 751
BssMI GATC 2 cut(s) 530, 772
BssNI GRCGYC 1 cut(s) 392
BssT1I CCWWGG 2 cut(s) 85, 290
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 277, 793
Bst6I CTCTTC 6 cut(s) 93, 97, 183, 201, 568, 752
BstACI GRCGYC 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 374, 553, 712
BstDEI CTNAG 8 cut(s) 116, 152, 262, 307, 366, 483, 579, 698
BstDSI CCRYGG 3 cut(s) 85, 290, 751
BstF5I GGATG 2 cut(s) 397, 673
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 2 cut(s) 533, 775
BstMAI GTCTC 2 cut(s) 150, 198
BstMBI GATC 2 cut(s) 530, 772
BstMWI GCNNNNNNNGC 3 cut(s) 369, 461, 642
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 2 cut(s) 24, 649
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 2 cut(s) 783, 792
BstV1I GCAGC 2 cut(s) 237, 240
BstV2I GAAGAC 1 cut(s) 288
Bsu36I CCTNAGG 1 cut(s) 307
BsuRI GGCC 2 cut(s) 174, 520
BtgI CCRYGG 3 cut(s) 85, 290, 751
BtsCI GGATG 2 cut(s) 397, 673
BtsIMutI CAGTG 1 cut(s) 706
BveI ACCTGC 1 cut(s) 755
Cac8I GCNNGC 3 cut(s) 374, 553, 712
CaiI CAGNNNCTG 2 cut(s) 373, 578
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 125, 335
CseI GACGC 1 cut(s) 381
Csp6I GTAC 2 cut(s) 211, 313
CviAII CATG 7 cut(s) 21, 86, 291, 646, 729, 866, 893
CviQI GTAC 2 cut(s) 211, 313
DdeI CTNAG 8 cut(s) 116, 152, 262, 307, 366, 483, 579, 698
DpnI GATC 2 cut(s) 532, 774
DpnII GATC 2 cut(s) 530, 772
DriI GACNNNNNGTC 1 cut(s) 333
Eam1104I CTCTTC 6 cut(s) 93, 97, 183, 201, 568, 752
Eam1105I GACNNNNNGTC 1 cut(s) 333
EarI CTCTTC 6 cut(s) 93, 97, 183, 201, 568, 752
Eco130I CCWWGG 2 cut(s) 85, 290
Eco24I GRGCYC 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 125, 335
Eco57I CTGAAG 2 cut(s) 78, 332
Eco81I CCTNAGG 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 85, 290
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 2 cut(s) 85, 290
FaeI CATG 7 cut(s) 24, 89, 294, 649, 732, 869, 896
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 659
FatI CATG 7 cut(s) 20, 85, 290, 645, 728, 865, 892
FbaI TGATCA 1 cut(s) 772
FblI GTMKAC 2 cut(s) 420, 693
Fnu4HI GCNGC 2 cut(s) 226, 229
FokI GGATG 2 cut(s) 384, 680
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 2 cut(s) 226, 229
FspBI CTAG 2 cut(s) 600, 887
GlaI GCGC 1 cut(s) 433
GluI GCNGC 2 cut(s) 226, 229
GsuI CTGGAG 3 cut(s) 114, 151, 489
HaeIII GGCC 2 cut(s) 174, 520
HgaI GACGC 1 cut(s) 381
HhaI GCGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 392
Hin1II CATG 7 cut(s) 24, 89, 294, 649, 732, 869, 896
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 5 cut(s) 43, 191, 544, 581, 672
HphI GGTGA 2 cut(s) 348, 373
Hpy166II GTNNAC 2 cut(s) 421, 694
Hpy188I TCNGA 6 cut(s) 97, 153, 265, 369, 486, 540
Hpy188III TCNNGA 3 cut(s) 35, 137, 168
Hpy8I GTNNAC 2 cut(s) 421, 694
HpyAV CCTTC 3 cut(s) 7, 348, 382
HpyCH4III ACNGT 2 cut(s) 277, 793
HpyCH4V TGCA 6 cut(s) 4, 408, 427, 455, 764, 812
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 461, 642
HpyF3I CTNAG 8 cut(s) 116, 152, 262, 307, 366, 483, 579, 698
Hsp92I GRCGYC 1 cut(s) 392
Hsp92II CATG 7 cut(s) 24, 89, 294, 649, 732, 869, 896
HspAI GCGC 1 cut(s) 432
KpnI GGTACC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 2 cut(s) 530, 772
LguI GCTCTTC 2 cut(s) 97, 568
LmnI GCTCC 5 cut(s) 112, 502, 508, 762, 824
Lsp1109I GCAGC 2 cut(s) 237, 240
MaeI CTAG 2 cut(s) 600, 887
MaeIII GTNAC 6 cut(s) 379, 393, 466, 582, 787, 796
MalI GATC 2 cut(s) 532, 774
MboI GATC 2 cut(s) 530, 772
MhlI GDGCHC 2 cut(s) 557, 759
MluCI AATT 6 cut(s) 107, 525, 814, 846, 854, 907
MlyI GAGTC 3 cut(s) 200, 590, 681
MseI TTAA 2 cut(s) 618, 906
MslI CAYNNNNRTG 1 cut(s) 650
MspA1I CMGCKG 2 cut(s) 464, 636
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 369, 461, 642
NcoI CCATGG 2 cut(s) 85, 290
NdeII GATC 2 cut(s) 530, 772
NlaIII CATG 7 cut(s) 24, 89, 294, 649, 732, 869, 896
NlaIV GGNNCC 3 cut(s) 314, 609, 758
NmuCI GTSAC 5 cut(s) 379, 393, 466, 582, 796
NspI RCATGY 2 cut(s) 24, 649
PciSI GCTCTTC 2 cut(s) 97, 568
PfeI GAWTC 2 cut(s) 43, 544
PkrI GCNGC 2 cut(s) 227, 230
PleI GAGTC 3 cut(s) 199, 589, 680
PpsI GAGTC 3 cut(s) 199, 589, 680
PpuMI RGGWCCY 1 cut(s) 335
PshBI ATTAAT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 3 cut(s) 314, 609, 758
PspPI GGNCC 2 cut(s) 125, 335
PspPPI RGGWCCY 1 cut(s) 335
PstNI CAGNNNCTG 2 cut(s) 373, 578
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 212, 314
RsaNI GTAC 2 cut(s) 211, 313
RseI CAYNNNNRTG 1 cut(s) 650
SapI GCTCTTC 2 cut(s) 97, 568
SaqAI TTAA 2 cut(s) 618, 906
SatI GCNGC 2 cut(s) 226, 229
Sau3AI GATC 2 cut(s) 530, 772
Sau96I GGNCC 2 cut(s) 125, 335
SchI GAGTC 3 cut(s) 200, 590, 681
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 2 cut(s) 557, 759
SfcI CTRYAG 2 cut(s) 783, 792
SinI GGWCC 2 cut(s) 125, 335
SmiMI CAYNNNNRTG 1 cut(s) 650
Sse9I AATT 6 cut(s) 107, 525, 814, 846, 854, 907
SsiI CCGC 2 cut(s) 416, 636
SspMI CTAG 2 cut(s) 600, 887
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 2 cut(s) 85, 290
TaaI ACNGT 2 cut(s) 277, 793
TaqI TCGA 1 cut(s) 529
TasI AATT 6 cut(s) 107, 525, 814, 846, 854, 907
TfiI GAWTC 2 cut(s) 43, 544
Tru1I TTAA 2 cut(s) 618, 906
Tru9I TTAA 2 cut(s) 618, 906
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 5 cut(s) 379, 393, 466, 582, 796
TseI GCWGC 2 cut(s) 225, 228
Tsp45I GTSAC 5 cut(s) 379, 393, 466, 582, 796
TspDTI ATGAA 4 cut(s) 26, 756, 847, 881
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 1 cut(s) 706
VpaK11BI GGWCC 2 cut(s) 125, 335
VspI ATTAAT 1 cut(s) 906
XceI RCATGY 2 cut(s) 24, 649
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 513
XmiI GTMKAC 2 cut(s) 420, 693
XspI CTAG 2 cut(s) 600, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.