Rroxscaffold_2G00104590

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010805
Physical Location & Seq
Reverse (-)
27733265 .. 27737906
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104590.1

Sequence Viewer

Length: 1008 bp
ATGCAAAAGATGGAGGAGGCATGTCCTCAACTTCTGGATTTGATTCCCAAAGAAAGGGAGTGGCTTGTGAGCAAAGATGACAGCCATGGCTCTTCAGAAGAGAAGAAATTGGAGCTAAGGCTTGGTCCTCCAGGTGAAGACTGGTCAAATCTGAGAGACAACACCTCCAGAGGCCACAAAGAAAGAGATGAGTCTCTTCTTTCTCTTGGGTACTTCTACAACAACAGCAGCAATGGAAACCAAACCCACCATTTTGCTTCCTCAGAGAGAAAGACTGTTCTGCCCTCTCCATGGTCTTCTTATCCCTCAGGGTACCACCACAACCAGACAAAAGGTCCTTCCTTTCTTCAGTTCTCACCAAGCTCTCAGAGCCTGCCTGTCACCCAGAAGGCGTCACATCCCTGTTGCACTAAAGCGGTAGACTTGCAGAGCGCAGAGAAGAAGAAAGCTTCTGCAAATCCAGCTGTGACCAACAACACTTCTCAGAAAAGAACTGCTCCTGCTCCAGTTGTGGGTTGGCCTCCAATTCGATCTTTTCGGAAGAATCTTGCGAGCACCAGCTCTTCAAAACCAGCACTTGAGTCACAAAAGGTAGCCCCTAGCAAGGTTCCTAATGTTAAACCAGTCGAAACCAGCGGAAAAGGCATGTTTGTGAAAATCAATATGGATGGAGTCCCCATTGGACGAAAAGTAGACCTCAGTGCTTATGCCAGCTACGAAAAGCTCTCCTCTGCTGTTGATGAACTCTTCCGTGGGCTCCTTGCAGCTCAAAGAGATTCTGGTGCTGGTGGAATCAAGAACAAGATAGAAGACGAGAAAGAAATTACAGGTTTATTGGATGGAAGTGGAGAATATACTCTCGTATATGAAGATAATGAGGGTGACAGGATGCTTGTTGGGGATGTCCCATGGCACATGTTTGTGTCTACTGTGAAGAGGTTGCGTGTGCTCAAGAGCTCTGAACTTTCTGCACTTAATATTCGCAGCGGTAAAGAACTGAAGAACTGA

Protein Analysis

335

Amino Acids

36.87

Weight (kDa)

9.01

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 37 - 326 1.9e-68 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0010805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51950 AT3G16500 AT3G16500
fragaria_vesca FvH4_6g32450 FvH4_6g32450
malus_domestica MD09G1202300.v1.1 MD17G1183500.v1.1
prunus_persica Prupe.3G058600_v2.0.a1
pyrus_communis pycom09g11930 pycom17g19260
rosa_chinensis RchiOBHm_Chr2g0140681
rosa_laevigata RLG00000019891
rosa_multiflora Rmu_sc0003342.1_g000011
rosa_roxburghii Rroxscaffold_2G00104590
rosa_rugosa Rorug02G0364000
rosa_samantha Rh2AG415700 Rh2BG425200 Rh2CG401700 Rh2DG435200
rosa_wichuraiana Rw2G034010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 3 cut(s) 420, 693, 926
AciI CCGC 3 cut(s) 416, 636, 987
AcuI CTGAAG 2 cut(s) 78, 332
AcyI GRCGYC 1 cut(s) 392
AfaI GTAC 2 cut(s) 212, 314
AfiI CCNNNNNNNGG 4 cut(s) 54, 291, 512, 604
AflIII ACRYGT 1 cut(s) 915
AgsI TTSAA 1 cut(s) 567
AhdI GACNNNNNGTC 1 cut(s) 333
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 9 cut(s) 115, 363, 449, 464, 561, 714, 724, 767, 957
AluI AGCT 9 cut(s) 115, 363, 449, 464, 561, 714, 724, 767, 957
Alw21I GWGCWC 3 cut(s) 557, 951, 959
Alw26I GTCTC 2 cut(s) 150, 198
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 2 cut(s) 172, 518
ApeKI GCWGC 3 cut(s) 228, 764, 984
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 2 cut(s) 125, 335
AsuHPI GGTGA 4 cut(s) 146, 348, 373, 893
AvaII GGWCC 2 cut(s) 125, 335
AxyI CCTNAGG 1 cut(s) 307
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 2 cut(s) 759, 959
BbsI GAAGAC 3 cut(s) 144, 288, 816
Bbv12I GWGCWC 3 cut(s) 557, 951, 959
BbvI GCAGC 3 cut(s) 240, 776, 996
BccI CCATC 3 cut(s) 4, 662, 833
BciT130I CCWGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 150, 198
BfaI CTAG 1 cut(s) 600
BisI GCNGC 3 cut(s) 229, 765, 985
BlsI GCNGC 3 cut(s) 230, 766, 986
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 125, 335
BmeRI GACNNNNNGTC 1 cut(s) 333
BmgT120I GGNCC 2 cut(s) 125, 335
BmiI GGNNCC 3 cut(s) 314, 609, 758
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 879
BpiI GAAGAC 3 cut(s) 144, 288, 816
BpmI CTGGAG 3 cut(s) 114, 151, 489
Bpu10I CCTNAGC 1 cut(s) 116
BpuEI CTTGAG 2 cut(s) 599, 935
BsaHI GRCGYC 1 cut(s) 392
BsaJI CCNNGG 4 cut(s) 85, 290, 751, 908
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 291, 512, 604
Bse1I ACTGG 3 cut(s) 146, 506, 623
Bse21I CCTNAGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 4 cut(s) 85, 290, 751, 908
BseGI GGATG 5 cut(s) 397, 673, 844, 894, 907
BseLI CCNNNNNNNGG 4 cut(s) 54, 291, 512, 604
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 6 cut(s) 143, 276, 321, 380, 497, 712
BseNI ACTGG 3 cut(s) 146, 506, 623
BseRI GAGGAG 2 cut(s) 29, 718
BseXI GCAGC 3 cut(s) 240, 776, 996
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 2 cut(s) 174, 520
BshNI GGYRCC 1 cut(s) 312
BsiHKAI GWGCWC 3 cut(s) 557, 951, 959
BslFI GGGAC 2 cut(s) 659, 890
BslI CCNNNNNNNGG 4 cut(s) 54, 291, 512, 604
BsmAI GTCTC 2 cut(s) 150, 198
BsmFI GGGAC 2 cut(s) 659, 890
BsnI GGCC 2 cut(s) 174, 520
Bsp1286I GDGCHC 4 cut(s) 557, 759, 951, 959
Bsp143I GATC 1 cut(s) 530
Bsp19I CCATGG 3 cut(s) 85, 290, 908
BspACI CCGC 3 cut(s) 416, 636, 987
BspANI GGCC 2 cut(s) 174, 520
BspCNI CTCAG 6 cut(s) 144, 275, 320, 379, 496, 711
BspLI GGNNCC 3 cut(s) 314, 609, 758
BspQI GCTCTTC 2 cut(s) 97, 568
BspT107I GGYRCC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 238
BsrI ACTGG 3 cut(s) 146, 506, 623
BssECI CCNNGG 4 cut(s) 85, 290, 751, 908
BssMI GATC 1 cut(s) 530
BssNI GRCGYC 1 cut(s) 392
BssT1I CCWWGG 3 cut(s) 85, 290, 908
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 277, 931
Bst6I CTCTTC 6 cut(s) 93, 97, 201, 568, 752, 929
BstACI GRCGYC 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 374, 553, 712
BstDEI CTNAG 7 cut(s) 116, 152, 262, 307, 366, 483, 698
BstDSI CCRYGG 4 cut(s) 85, 290, 751, 908
BstF5I GGATG 5 cut(s) 397, 673, 844, 894, 907
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 1 cut(s) 533
BstMAI GTCTC 2 cut(s) 150, 198
BstMBI GATC 1 cut(s) 530
BstMWI GCNNNNNNNGC 3 cut(s) 369, 461, 642
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 3 cut(s) 24, 649, 919
BstSCI CCNGG 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 240, 776, 996
BstV2I GAAGAC 3 cut(s) 144, 288, 816
Bsu36I CCTNAGG 1 cut(s) 307
BsuRI GGCC 2 cut(s) 174, 520
BtgI CCRYGG 4 cut(s) 85, 290, 751, 908
BtsCI GGATG 5 cut(s) 397, 673, 844, 894, 907
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 3 cut(s) 374, 553, 712
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 125, 335
CseI GACGC 1 cut(s) 381
Csp6I GTAC 2 cut(s) 211, 313
CviAII CATG 6 cut(s) 21, 86, 291, 646, 909, 916
CviQI GTAC 2 cut(s) 211, 313
DdeI CTNAG 7 cut(s) 116, 152, 262, 307, 366, 483, 698
DpnI GATC 1 cut(s) 532
DpnII GATC 1 cut(s) 530
DriI GACNNNNNGTC 1 cut(s) 333
Eam1104I CTCTTC 6 cut(s) 93, 97, 201, 568, 752, 929
Eam1105I GACNNNNNGTC 1 cut(s) 333
EarI CTCTTC 6 cut(s) 93, 97, 201, 568, 752, 929
Ecl136II GAGCTC 1 cut(s) 957
Eco130I CCWWGG 3 cut(s) 85, 290, 908
Eco24I GRGCYC 2 cut(s) 759, 959
Eco47I GGWCC 2 cut(s) 125, 335
Eco53kI GAGCTC 1 cut(s) 957
Eco57I CTGAAG 2 cut(s) 78, 332
Eco81I CCTNAGG 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 3 cut(s) 85, 290, 908
EcoT38I GRGCYC 2 cut(s) 759, 959
ErhI CCWWGG 3 cut(s) 85, 290, 908
FaeI CATG 6 cut(s) 24, 89, 294, 649, 912, 919
FaqI GGGAC 2 cut(s) 659, 890
FatI CATG 6 cut(s) 20, 85, 290, 645, 908, 915
FblI GTMKAC 3 cut(s) 420, 693, 926
Fnu4HI GCNGC 3 cut(s) 229, 765, 985
FokI GGATG 5 cut(s) 384, 680, 851, 901, 914
FriOI GRGCYC 2 cut(s) 759, 959
Fsp4HI GCNGC 3 cut(s) 229, 765, 985
FspBI CTAG 1 cut(s) 600
GlaI GCGC 1 cut(s) 433
GluI GCNGC 3 cut(s) 229, 765, 985
GsuI CTGGAG 3 cut(s) 114, 151, 489
HaeIII GGCC 2 cut(s) 174, 520
HgaI GACGC 1 cut(s) 381
HhaI GCGC 1 cut(s) 434
Hin1I GRCGYC 1 cut(s) 392
Hin1II CATG 6 cut(s) 24, 89, 294, 649, 912, 919
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 7 cut(s) 43, 191, 544, 581, 672, 776, 792
HphI GGTGA 4 cut(s) 146, 348, 373, 893
Hpy166II GTNNAC 3 cut(s) 421, 694, 927
Hpy188I TCNGA 7 cut(s) 97, 153, 265, 369, 486, 540, 961
Hpy188III TCNNGA 4 cut(s) 35, 168, 796, 952
Hpy8I GTNNAC 3 cut(s) 421, 694, 927
HpyAV CCTTC 2 cut(s) 348, 382
HpyCH4III ACNGT 2 cut(s) 277, 931
HpyCH4V TGCA 6 cut(s) 4, 408, 427, 455, 764, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 461, 642
HpyF3I CTNAG 7 cut(s) 116, 152, 262, 307, 366, 483, 698
Hsp92I GRCGYC 1 cut(s) 392
Hsp92II CATG 6 cut(s) 24, 89, 294, 649, 912, 919
HspAI GCGC 1 cut(s) 432
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 1 cut(s) 530
LguI GCTCTTC 2 cut(s) 97, 568
LmnI GCTCC 4 cut(s) 112, 502, 508, 762
Lsp1109I GCAGC 3 cut(s) 240, 776, 996
LweI GCATC 1 cut(s) 879
MaeI CTAG 1 cut(s) 600
MaeIII GTNAC 5 cut(s) 379, 393, 466, 582, 881
MalI GATC 1 cut(s) 532
MboI GATC 1 cut(s) 530
MhlI GDGCHC 4 cut(s) 557, 759, 951, 959
MluCI AATT 3 cut(s) 107, 525, 822
MlyI GAGTC 3 cut(s) 200, 590, 681
MseI TTAA 2 cut(s) 618, 975
MslI CAYNNNNRTG 2 cut(s) 650, 920
MspA1I CMGCKG 3 cut(s) 464, 636, 987
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 369, 461, 642
NcoI CCATGG 3 cut(s) 85, 290, 908
NdeII GATC 1 cut(s) 530
NlaIII CATG 6 cut(s) 24, 89, 294, 649, 912, 919
NlaIV GGNNCC 3 cut(s) 314, 609, 758
NmuCI GTSAC 5 cut(s) 379, 393, 466, 582, 881
NspI RCATGY 3 cut(s) 24, 649, 919
PciI ACATGT 1 cut(s) 915
PciSI GCTCTTC 2 cut(s) 97, 568
PfeI GAWTC 4 cut(s) 43, 544, 776, 792
PkrI GCNGC 3 cut(s) 230, 766, 986
PleI GAGTC 3 cut(s) 199, 589, 680
PpsI GAGTC 3 cut(s) 199, 589, 680
PpuMI RGGWCCY 1 cut(s) 335
PscI ACATGT 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 3 cut(s) 314, 609, 758
PspPI GGNCC 2 cut(s) 125, 335
PspPPI RGGWCCY 1 cut(s) 335
PstNI CAGNNNCTG 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 212, 314
RsaNI GTAC 2 cut(s) 211, 313
RseI CAYNNNNRTG 2 cut(s) 650, 920
SacI GAGCTC 1 cut(s) 959
SapI GCTCTTC 2 cut(s) 97, 568
SaqAI TTAA 2 cut(s) 618, 975
SatI GCNGC 3 cut(s) 229, 765, 985
Sau3AI GATC 1 cut(s) 530
Sau96I GGNCC 2 cut(s) 125, 335
SchI GAGTC 3 cut(s) 200, 590, 681
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 4 cut(s) 557, 759, 951, 959
SfaNI GCATC 1 cut(s) 879
SinI GGWCC 2 cut(s) 125, 335
SmiMI CAYNNNNRTG 2 cut(s) 650, 920
SmlI CTYRAG 2 cut(s) 578, 950
SmoI CTYRAG 2 cut(s) 578, 950
Sse9I AATT 3 cut(s) 107, 525, 822
SsiI CCGC 3 cut(s) 416, 636, 987
SspI AATATT 1 cut(s) 979
SspMI CTAG 1 cut(s) 600
SstI GAGCTC 1 cut(s) 959
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 3 cut(s) 85, 290, 908
TaaI ACNGT 2 cut(s) 277, 931
TaqI TCGA 2 cut(s) 529, 627
TasI AATT 3 cut(s) 107, 525, 822
TfiI GAWTC 4 cut(s) 43, 544, 776, 792
Tru1I TTAA 2 cut(s) 618, 975
Tru9I TTAA 2 cut(s) 618, 975
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 5 cut(s) 379, 393, 466, 582, 881
TseI GCWGC 3 cut(s) 228, 764, 984
Tsp45I GTSAC 5 cut(s) 379, 393, 466, 582, 881
TspDTI ATGAA 2 cut(s) 756, 882
TspGWI ACGGA 1 cut(s) 740
TspRI CASTG 1 cut(s) 706
VpaK11BI GGWCC 2 cut(s) 125, 335
XceI RCATGY 3 cut(s) 24, 649, 919
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 513
XmiI GTMKAC 3 cut(s) 420, 693, 926
XspI CTAG 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.