AT3G16510

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009797
Physical Location & Seq
Reverse (-)
5616801 .. 5618322
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16510.1

Sequence Viewer

Length: 1083 bp
ATGGCGAATCTTACCTTAGAGCTCAACGTTTACTCAGCAAAAGATCTTGAAAATGTCAACCTCATCACAAAAATGGACGTATACGCCGTCGTTTGGATCACCGGCGATGATTCTCGGAAGAATCATAAGGAGAAAACCCCCATCGATCGCACCGGAGAATCTGAGCCTACTTGGAATCACACCGTCAAATTTTCCGTTGATCAAAGGTTAGCACACGAAGGTCGTTTAACCCTCGTCGTGAAATTGGTCTGCGATCGGATCTTCGGCGACAAAGATCTCGGAGAGGTCCAAGTCCCGGTTCTTGAGCTTCTTCATGGTTCTTCGTCGCCGTCATCTAACGGTGACGGTCAGGGGATGATGAGATTTGTTACGTATCAGGTCAGAACTCCGTTTGGAAAAGGTCAAGGCTCGTTGACTTTTTCGTACCGGTTCGATTCACCGACATTTAAACCGGATCAACCGGTTTCATCACACGTTTTCCACCAGGATCCACCGGTATCATCATCACACGTTTACACGAATCCGATGGATATACCGTCTGATTTCTCGTCGGCGACGACAAATTATCCTCCGCCGCAATCGTCTGAAGCAAATTTCTATCCACCATTATCATCGATTGGATATCCTCCGTCGTCTCCGCCGCAACATTATTCCTCACCGCCGTACCCGTACCCGAACCCGTATCAGTACCATTCTCATTACCCGGAACAGCCAGTCGCTGTTTATCCACCTCCGCCACCCTCTGCATCGAATTTATATCCTCCGCCGTATTACAGTACTTCTCCGCCGCAGCATCAATCCTATCCGCCGCCTCCCGGCCATTCGTTCCACCAAACGCAACCGTCACAGTCGTTTCACGGGTTCGCGCCTTCGTCTCCGCAGAATCAGCACGGGTATGGTTATCCGCCGCCGACGTCACCGGGATATGGATACGGTTGTCCGACTACACAAGTGCCGCCGAAGAATAATAATAATAAACCAGGGCTTGGGCTTGGGCTTGGAGTGGGAGCTGGGCTTCTGGGAGGAGCATTGGGTGGGCTCTTAATCAGTGATATAGTATCCGACATTGGTTTTGATTTTTAA

Protein Analysis

360

Amino Acids

39.29

Weight (kDa)

5.97

Isoelectric Point (pI)

56.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 107 1.9e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 917
AccB7I CCANNNNNTGG 1 cut(s) 986
AccI GTMKAC 1 cut(s) 81
AccII CGCG 1 cut(s) 866
AclI AACGTT 1 cut(s) 27
AclWI GGATC 5 cut(s) 104, 266, 462, 482, 495
AcoI YGGCCR 1 cut(s) 817
AcsI RAATTY 3 cut(s) 188, 592, 751
AcuI CTGAAG 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 914
AfaI GTAC 5 cut(s) 425, 665, 671, 689, 778
AfiI CCNNNNNNNGG 5 cut(s) 93, 295, 815, 926, 986
AflIII ACRYGT 2 cut(s) 472, 508
AgeI ACCGGT 3 cut(s) 426, 460, 493
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 2 cut(s) 483, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 3 cut(s) 22, 307, 1010
AluI AGCT 3 cut(s) 22, 307, 1010
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 639, 879
AlwI GGATC 5 cut(s) 104, 266, 462, 482, 495
AlwNI CAGNNNCTG 1 cut(s) 719
AoxI GGCC 1 cut(s) 817
ApeKI GCWGC 1 cut(s) 790
ApoI RAATTY 3 cut(s) 188, 592, 751
ArsI GACNNNNNNTTYG 1 cut(s) 1055
AsiGI ACCGGT 3 cut(s) 426, 460, 493
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 1 cut(s) 286
AsuC2I CCSGG 4 cut(s) 296, 704, 816, 921
AsuHPI GGTGA 5 cut(s) 91, 353, 429, 648, 909
AvaII GGWCC 1 cut(s) 286
BaeI ACNNNNGTAYC 4 cut(s) 647, 680, 922, 955
BamHI GGATCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 24, 1041
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 802
BccI CCATC 2 cut(s) 149, 520
BceAI ACGGC 4 cut(s) 71, 313, 646, 751
BciT130I CCWGG 2 cut(s) 485, 981
BciVI GTATCC 2 cut(s) 923, 1069
BclI TGATCA 1 cut(s) 199
BcnI CCSGG 4 cut(s) 296, 704, 816, 921
BcoDI GTCTC 2 cut(s) 639, 879
BfuI GTATCC 2 cut(s) 923, 1069
BglII AGATCT 2 cut(s) 43, 274
BisI GCNGC 7 cut(s) 575, 641, 788, 791, 809, 908, 956
BlsI GCNGC 7 cut(s) 576, 642, 789, 792, 810, 909, 957
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 6 cut(s) 296, 485, 704, 816, 921, 981
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 6 cut(s) 296, 485, 704, 816, 921, 981
BmsI GCATC 2 cut(s) 755, 802
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 4 cut(s) 296, 704, 816, 921
Bsa29I ATCGAT 2 cut(s) 144, 614
BsaAI YACGTR 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 914
BsaJI CCNNGG 1 cut(s) 980
BsaWI WCCGGW 5 cut(s) 152, 426, 451, 460, 493
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 295, 815, 926, 986
Bse118I RCCGGY 4 cut(s) 101, 426, 460, 493
Bse1I ACTGG 1 cut(s) 713
BseBI CCWGG 2 cut(s) 485, 981
BseCI ATCGAT 2 cut(s) 144, 614
BseDI CCNNGG 1 cut(s) 980
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 5 cut(s) 93, 295, 815, 926, 986
BseMII CTCAG 2 cut(s) 48, 153
BseNI ACTGG 1 cut(s) 713
BseRI GAGGAG 1 cut(s) 1038
BseXI GCAGC 1 cut(s) 802
BseYI CCCAGC 1 cut(s) 1010
Bsh1236I CGCG 1 cut(s) 866
Bsh1285I CGRYCG 2 cut(s) 148, 256
BshFI GGCC 1 cut(s) 819
BshTI ACCGGT 3 cut(s) 426, 460, 493
BshVI ATCGAT 2 cut(s) 144, 614
BsiEI CGRYCG 2 cut(s) 148, 256
BsiHKAI GWGCWC 1 cut(s) 24
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 5 cut(s) 93, 295, 815, 926, 986
BsmAI GTCTC 2 cut(s) 639, 879
BsmBI CGTCTC 2 cut(s) 639, 879
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 819
Bsp1286I GDGCHC 2 cut(s) 24, 1041
Bsp143I GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
BspANI GGCC 1 cut(s) 819
BspCNI CTCAG 2 cut(s) 47, 154
BspDI ATCGAT 2 cut(s) 144, 614
BspFNI CGCG 1 cut(s) 866
BspLI GGNNCC 1 cut(s) 489
BspPI GGATC 5 cut(s) 104, 266, 462, 482, 495
BsrFI RCCGGY 4 cut(s) 101, 426, 460, 493
BsrI ACTGG 1 cut(s) 713
BssAI RCCGGY 4 cut(s) 101, 426, 460, 493
BssECI CCNNGG 1 cut(s) 980
BssMI GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
BssNAI GTATAC 1 cut(s) 82
BssNI GRCGYC 1 cut(s) 914
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 485, 981
Bst4CI ACNGT 8 cut(s) 184, 341, 347, 537, 776, 843, 849, 935
BstACI GRCGYC 1 cut(s) 914
BstBAI YACGTR 1 cut(s) 372
BstDEI CTNAG 3 cut(s) 16, 34, 162
BstF5I GGATG 1 cut(s) 360
BstFNI CGCG 1 cut(s) 866
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 9 cut(s) 46, 99, 148, 202, 256, 261, 277, 457, 490
BstMAI GTCTC 2 cut(s) 639, 879
BstMBI GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
BstMCI CGRYCG 2 cut(s) 148, 256
BstMWI GCNNNNNNNGC 1 cut(s) 886
BstNI CCWGG 2 cut(s) 485, 981
BstSCI CCNGG 6 cut(s) 294, 483, 702, 814, 919, 979
BstSNI TACGTA 1 cut(s) 372
BstUI CGCG 1 cut(s) 866
BstV1I GCAGC 1 cut(s) 802
BstX2I RGATCY 4 cut(s) 43, 258, 274, 487
BstYI RGATCY 4 cut(s) 43, 258, 274, 487
BstZ17I GTATAC 1 cut(s) 82
Bsu15I ATCGAT 2 cut(s) 144, 614
BsuI GTATCC 2 cut(s) 923, 1069
BsuRI GGCC 1 cut(s) 819
BsuTUI ATCGAT 2 cut(s) 144, 614
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 1054
CaiI CAGNNNCTG 1 cut(s) 719
CfoI GCGC 1 cut(s) 868
Cfr10I RCCGGY 4 cut(s) 101, 426, 460, 493
Cfr13I GGNCC 1 cut(s) 286
ClaI ATCGAT 2 cut(s) 144, 614
Csp6I GTAC 5 cut(s) 424, 664, 670, 688, 777
CspAI ACCGGT 3 cut(s) 426, 460, 493
CviAII CATG 1 cut(s) 314
CviQI GTAC 5 cut(s) 424, 664, 670, 688, 777
DdeI CTNAG 3 cut(s) 16, 34, 162
DpnI GATC 9 cut(s) 45, 98, 147, 201, 255, 260, 276, 456, 489
DpnII GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
DraI TTTAAA 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 817
EciI GGCGGA 7 cut(s) 561, 627, 723, 753, 774, 795, 894
Ecl136II GAGCTC 1 cut(s) 22
Eco105I TACGTA 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 24, 1041
Eco32I GATATC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 606
EcoICRI GAGCTC 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 483, 979
EcoRV GATATC 1 cut(s) 623
EcoT38I GRGCYC 2 cut(s) 24, 1041
Esp3I CGTCTC 2 cut(s) 639, 879
FaeI CATG 1 cut(s) 317
FaiI YATR 8 cut(s) 82, 126, 315, 533, 757, 897, 927, 1055
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 199
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 7 cut(s) 575, 641, 788, 791, 809, 908, 956
FokI GGATG 1 cut(s) 367
FriOI GRGCYC 2 cut(s) 24, 1041
Fsp4HI GCNGC 7 cut(s) 575, 641, 788, 791, 809, 908, 956
GlaI GCGC 1 cut(s) 867
GluI GCNGC 7 cut(s) 575, 641, 788, 791, 809, 908, 956
GsaI CCCAGC 1 cut(s) 1014
HaeIII GGCC 1 cut(s) 819
HhaI GCGC 1 cut(s) 868
Hin1I GRCGYC 1 cut(s) 914
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HincII GTYRAC 2 cut(s) 58, 414
HindII GTYRAC 2 cut(s) 58, 414
HinfI GANTC 8 cut(s) 7, 110, 121, 158, 175, 434, 520, 883
HphI GGTGA 5 cut(s) 91, 353, 429, 648, 909
Hpy166II GTNNAC 5 cut(s) 31, 58, 82, 414, 514
Hpy188III TCNNGA 3 cut(s) 47, 238, 302
Hpy8I GTNNAC 5 cut(s) 31, 58, 82, 414, 514
Hpy99I CGWCG 7 cut(s) 92, 239, 328, 553, 559, 634, 916
HpyAV CCTTC 2 cut(s) 212, 879
HpyCH4III ACNGT 8 cut(s) 184, 341, 347, 537, 776, 843, 849, 935
HpyCH4IV ACGT 6 cut(s) 27, 78, 371, 474, 510, 914
HpyCH4V TGCA 1 cut(s) 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 886
HpyF3I CTNAG 3 cut(s) 16, 34, 162
HpySE526I ACGT 6 cut(s) 27, 78, 371, 474, 510, 914
Hsp92I GRCGYC 1 cut(s) 914
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 1 cut(s) 866
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
LmnI GCTCC 2 cut(s) 1007, 1025
Lsp1109I GCAGC 1 cut(s) 802
LweI GCATC 2 cut(s) 755, 802
MaeII ACGT 6 cut(s) 27, 78, 371, 474, 510, 914
MaeIII GTNAC 4 cut(s) 341, 367, 843, 915
MalI GATC 9 cut(s) 45, 98, 147, 201, 255, 260, 276, 456, 489
MboI GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
MboII GAAGA 5 cut(s) 130, 253, 302, 312, 973
MflI RGATCY 4 cut(s) 43, 258, 274, 487
MhlI GDGCHC 2 cut(s) 24, 1041
MluCI AATT 5 cut(s) 188, 242, 562, 592, 751
MmeI TCCRAC 1 cut(s) 965
MseI TTAA 4 cut(s) 227, 447, 1043, 1081
MslI CAYNNNNRTG 2 cut(s) 71, 894
MspR9I CCNGG 6 cut(s) 296, 485, 704, 816, 921, 981
MvaI CCWGG 2 cut(s) 485, 981
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 1 cut(s) 886
NciI CCSGG 4 cut(s) 296, 704, 816, 921
NdeII GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 1 cut(s) 489
NmuCI GTSAC 3 cut(s) 341, 843, 915
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 554
PfeI GAWTC 8 cut(s) 7, 110, 121, 158, 175, 434, 520, 883
PflMI CCANNNNNTGG 1 cut(s) 986
PinAI ACCGGT 3 cut(s) 426, 460, 493
PkrI GCNGC 7 cut(s) 576, 642, 789, 792, 810, 909, 957
Ple19I CGATCG 2 cut(s) 148, 256
Ppu21I YACGTR 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 24
Psp1406I AACGTT 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 483, 979
PspFI CCCAGC 1 cut(s) 1010
PspGI CCWGG 2 cut(s) 483, 979
PspN4I GGNNCC 1 cut(s) 489
PspPI GGNCC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 719
PsuI RGATCY 4 cut(s) 43, 258, 274, 487
PvuI CGATCG 2 cut(s) 148, 256
RsaI GTAC 5 cut(s) 425, 665, 671, 689, 778
RsaNI GTAC 5 cut(s) 424, 664, 670, 688, 777
RseI CAYNNNNRTG 2 cut(s) 71, 894
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 4 cut(s) 227, 447, 1043, 1081
SatI GCNGC 7 cut(s) 575, 641, 788, 791, 809, 908, 956
Sau3AI GATC 9 cut(s) 43, 96, 145, 199, 253, 258, 274, 454, 487
Sau96I GGNCC 1 cut(s) 286
ScaI AGTACT 1 cut(s) 778
ScrFI CCNGG 6 cut(s) 296, 485, 704, 816, 921, 981
SduI GDGCHC 2 cut(s) 24, 1041
SfaNI GCATC 2 cut(s) 755, 802
SgrAI CRCCGGYG 1 cut(s) 101
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 71, 894
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SnaBI TACGTA 1 cut(s) 372
Sse9I AATT 5 cut(s) 188, 242, 562, 592, 751
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 6 cut(s) 294, 483, 702, 814, 919, 979
TaaI ACNGT 8 cut(s) 184, 341, 347, 537, 776, 843, 849, 935
TaiI ACGT 6 cut(s) 30, 81, 374, 477, 513, 917
TaqI TCGA 4 cut(s) 144, 432, 614, 749
TasI AATT 5 cut(s) 188, 242, 562, 592, 751
TatI WGTACW 1 cut(s) 776
TauI GCSGC 6 cut(s) 577, 643, 790, 811, 910, 958
TfiI GAWTC 8 cut(s) 7, 110, 121, 158, 175, 434, 520, 883
Tru1I TTAA 4 cut(s) 227, 447, 1043, 1081
Tru9I TTAA 4 cut(s) 227, 447, 1043, 1081
TscAI CASTG 1 cut(s) 1054
TseFI GTSAC 3 cut(s) 341, 843, 915
TseI GCWGC 1 cut(s) 790
Tsp45I GTSAC 3 cut(s) 341, 843, 915
TspDTI ATGAA 2 cut(s) 302, 456
TspGWI ACGGA 3 cut(s) 184, 378, 618
TspRI CASTG 1 cut(s) 1054
Van91I CCANNNNNTGG 1 cut(s) 986
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 3 cut(s) 188, 592, 751
XmiI GTMKAC 1 cut(s) 81
ZraI GACGTC 1 cut(s) 915
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.