MD13G1169800.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009797
Physical Location & Seq
Reverse (-)
13760662 .. 13761543
882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1169800.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGAGCAGAGGATCCTAGAACTCGAACTCATCTCAGCCAAAGACATCAAAGACGTCAACCTCTTATCCAAGATGGACGTTTACGCCGTCGTATCCCTCCAGGGCGACGACGTATCTCGCGCCGGCAAGCAGAAAACCAAGACCAAAGTCTCCCAGGGATGCGGGACCCATCCCACCTGGAACTTCCACATGAAGTTCGTTCTCGACGAGTACCTAATTCAGCAGAACCGCATCTCCCTCGTCTTCAAGCTCGTCTGCCAGCGCAGCCTCGGCGACAAAGACATCGGCCAGGTCGTCGTCCCCGTTAAGGAGCTCTTGGATTCCGTAAAGGCTGACGCCGCCTCCATGAAGTTTGTCGCTTACCAAGTCAGAAAGCCCTCAGGGAAGCCGAAAGGTGAGCTCGAGTTCTCATACAAATTTGGTGACAAAGTGGCGGCTTCAGAGGCGACGCTGACGGCGGCTTCGTCAAAGGGTAATGAGCCGGTAACGGCCTATCCGGTGGTTGGACCGTCAGTCCAGCCGGTGGTGGGGTATGGTGGATACCCACCTCCAGTTGGCAGCTCTGCTGGGCCTCCGCCACCTGGATACGAGTACCCAGCGCCACCGCAGGGATACGGGTACCCGCCGCCGCCGCCGCCACCAGGGTATGGGTACCCACCACCGCCACCAGGGTATGGGTACCCGCCACCGCCACCAGGGTATGGGTACCCTGGGCCGCCATCTGCCGGGTATGGATACCCACCGATGCAGCAACCCCAGCCGGAAGCAAAGAAGAAGAACTTTGGGATGGGGCTGGGAGCTGGAGTGTTAGGAGGGTTACTAGGTGGGATGCTAATTGGAGATATGGTATCTGATGGCGGTGGATGTGGTGGCGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

294

Amino Acids

30.8

Weight (kDa)

8.51

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 108 8.3e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 4 cut(s) 618, 651, 678, 705
AccB1I GGYRCC 4 cut(s) 618, 651, 678, 705
AccB7I CCANNNNNTGG 4 cut(s) 523, 647, 674, 701
AccII CGCG 1 cut(s) 120
AclWI GGATC 2 cut(s) 7, 20
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 2 cut(s) 54, 336
AfaI GTAC 6 cut(s) 212, 593, 620, 653, 680, 707
AgsI TTSAA 1 cut(s) 247
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 9 cut(s) 99, 153, 176, 288, 580, 640, 667, 694, 709
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 5 cut(s) 250, 313, 400, 561, 800
AluI AGCT 5 cut(s) 250, 313, 400, 561, 800
Alw21I GWGCWC 2 cut(s) 315, 402
Alw26I GTCTC 1 cut(s) 154
AlwI GGATC 2 cut(s) 7, 20
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 4 cut(s) 286, 489, 569, 713
ApeKI GCWGC 3 cut(s) 264, 558, 748
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
Asp718I GGTACC 4 cut(s) 618, 651, 678, 705
AspLEI GCGC 3 cut(s) 122, 264, 601
AspS9I GGNCC 4 cut(s) 165, 506, 569, 713
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 2 cut(s) 407, 434
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 2 cut(s) 165, 506
AxyI CCTNAGG 1 cut(s) 379
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 4 cut(s) 618, 651, 678, 705
BanII GRGCYC 2 cut(s) 315, 402
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 315, 402
BbvI GCAGC 3 cut(s) 276, 570, 760
BccI CCATC 5 cut(s) 67, 177, 727, 781, 848
BceAI ACGGC 3 cut(s) 71, 471, 504
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 9 cut(s) 101, 155, 178, 290, 582, 642, 669, 696, 711
BciVI GTATCC 5 cut(s) 103, 533, 578, 605, 728
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 1 cut(s) 154
BfaI CTAG 2 cut(s) 17, 821
BfoI RGCGCY 1 cut(s) 602
BfuI GTATCC 5 cut(s) 103, 533, 578, 605, 728
Bme18I GGWCC 2 cut(s) 165, 506
BmeRI GACNNNNNGTC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 4 cut(s) 165, 506, 569, 713
BmiI GGNNCC 7 cut(s) 14, 166, 167, 620, 653, 680, 707
BmsI GCATC 4 cut(s) 149, 240, 735, 819
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 3 cut(s) 83, 534, 822
BpuMI CCSGG 1 cut(s) 727
BsaHI GRCGYC 2 cut(s) 54, 336
BsaJI CCNNGG 9 cut(s) 100, 153, 154, 268, 641, 668, 695, 709, 710
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 8 cut(s) 218, 248, 326, 356, 789, 819, 831, 861
Bse118I RCCGGY 3 cut(s) 122, 481, 520
Bse1I ACTGG 1 cut(s) 551
Bse21I CCTNAGG 1 cut(s) 379
BseBI CCWGG 9 cut(s) 101, 155, 178, 290, 582, 642, 669, 696, 711
BseDI CCNNGG 9 cut(s) 100, 153, 154, 268, 641, 668, 695, 709, 710
BseGI GGATG 5 cut(s) 164, 169, 792, 834, 869
BseMII CTCAG 2 cut(s) 48, 393
BseNI ACTGG 1 cut(s) 551
BseXI GCAGC 3 cut(s) 276, 570, 760
BseYI CCCAGC 4 cut(s) 566, 595, 756, 793
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 288, 491, 571, 715
BshNI GGYRCC 4 cut(s) 618, 651, 678, 705
BsiHKAI GWGCWC 2 cut(s) 315, 402
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 6 cut(s) 123, 482, 497, 521, 726, 761
BslFI GGGAC 2 cut(s) 178, 284
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 178, 284
BsnI GGCC 4 cut(s) 288, 491, 571, 715
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 315, 402
Bsp143I GATC 1 cut(s) 12
BspANI GGCC 4 cut(s) 288, 491, 571, 715
BspCNI CTCAG 2 cut(s) 47, 392
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 7 cut(s) 14, 166, 167, 620, 653, 680, 707
BspPI GGATC 2 cut(s) 7, 20
BspT107I GGYRCC 4 cut(s) 618, 651, 678, 705
BsrFI RCCGGY 3 cut(s) 122, 481, 520
BsrI ACTGG 1 cut(s) 551
BssAI RCCGGY 3 cut(s) 122, 481, 520
BssECI CCNNGG 9 cut(s) 100, 153, 154, 268, 641, 668, 695, 709, 710
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 2 cut(s) 54, 336
Bst2UI CCWGG 9 cut(s) 101, 155, 178, 290, 582, 642, 669, 696, 711
Bst4CI ACNGT 1 cut(s) 510
BstACI GRCGYC 2 cut(s) 54, 336
BstC8I GCNNGC 3 cut(s) 124, 128, 260
BstDEI CTNAG 2 cut(s) 34, 379
BstF5I GGATG 5 cut(s) 164, 169, 792, 834, 869
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 3 cut(s) 122, 264, 601
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 7 cut(s) 264, 270, 338, 443, 631, 634, 757
BstNI CCWGG 9 cut(s) 101, 155, 178, 290, 582, 642, 669, 696, 711
BstPAI GACNNNNGTC 1 cut(s) 146
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 276, 570, 760
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 379
BsuI GTATCC 5 cut(s) 103, 533, 578, 605, 728
BsuRI GGCC 4 cut(s) 288, 491, 571, 715
BtsCI GGATG 5 cut(s) 164, 169, 792, 834, 869
Cac8I GCNNGC 3 cut(s) 124, 128, 260
CfoI GCGC 3 cut(s) 122, 264, 601
Cfr10I RCCGGY 3 cut(s) 122, 481, 520
Cfr13I GGNCC 4 cut(s) 165, 506, 569, 713
CseI GACGC 2 cut(s) 344, 457
Csp6I GTAC 6 cut(s) 211, 592, 619, 652, 679, 706
CviAII CATG 2 cut(s) 190, 346
CviQI GTAC 6 cut(s) 211, 592, 619, 652, 679, 706
DdeI CTNAG 2 cut(s) 34, 379
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 313, 400
Eco24I GRGCYC 2 cut(s) 315, 402
Eco47I GGWCC 2 cut(s) 165, 506
Eco53kI GAGCTC 2 cut(s) 313, 400
Eco57I CTGAAG 1 cut(s) 423
Eco81I CCTNAGG 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 313, 400
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 9 cut(s) 99, 153, 176, 288, 580, 640, 667, 694, 709
EcoT38I GRGCYC 2 cut(s) 315, 402
FaeI CATG 2 cut(s) 193, 349
FaiI YATR 9 cut(s) 191, 347, 412, 534, 648, 675, 702, 732, 845
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 764, 796
FaqI GGGAC 2 cut(s) 178, 284
FatI CATG 2 cut(s) 189, 345
FauI CCCGC 3 cut(s) 155, 630, 690
FokI GGATG 5 cut(s) 156, 171, 799, 841, 876
FriOI GRGCYC 2 cut(s) 315, 402
FspBI CTAG 2 cut(s) 17, 821
GlaI GCGC 3 cut(s) 121, 263, 600
GsaI CCCAGC 4 cut(s) 570, 599, 760, 797
GsuI CTGGAG 3 cut(s) 83, 534, 822
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 4 cut(s) 288, 491, 571, 715
HapII CCGG 6 cut(s) 123, 482, 497, 521, 726, 761
HgaI GACGC 2 cut(s) 344, 457
HhaI GCGC 3 cut(s) 122, 264, 601
Hin1I GRCGYC 2 cut(s) 54, 336
Hin1II CATG 2 cut(s) 193, 349
Hin6I GCGC 3 cut(s) 120, 262, 599
HinP1I GCGC 3 cut(s) 120, 262, 599
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 1 cut(s) 320
HpaII CCGG 6 cut(s) 123, 482, 497, 521, 726, 761
HphI GGTGA 2 cut(s) 407, 434
Hpy166II GTNNAC 2 cut(s) 58, 82
Hpy188I TCNGA 3 cut(s) 371, 442, 853
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 2 cut(s) 58, 82
Hpy99I CGWCG 6 cut(s) 92, 110, 113, 209, 299, 451
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 3 cut(s) 54, 78, 111
HpyCH4V TGCA 1 cut(s) 748
HpyF10VI GCNNNNNNNGC 7 cut(s) 264, 270, 338, 443, 631, 634, 757
HpyF3I CTNAG 2 cut(s) 34, 379
HpySE526I ACGT 3 cut(s) 54, 78, 111
Hsp92I GRCGYC 2 cut(s) 54, 336
Hsp92II CATG 2 cut(s) 193, 349
HspAI GCGC 3 cut(s) 120, 262, 599
KflI GGGWCCC 1 cut(s) 165
KpnI GGTACC 4 cut(s) 622, 655, 682, 709
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 4, 310, 797
Lsp1109I GCAGC 3 cut(s) 276, 570, 760
LweI GCATC 4 cut(s) 149, 240, 735, 819
MaeI CTAG 2 cut(s) 17, 821
MaeII ACGT 3 cut(s) 54, 78, 111
MaeIII GTNAC 3 cut(s) 422, 484, 816
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 3 cut(s) 235, 784, 787
MflI RGATCY 1 cut(s) 12
MhlI GDGCHC 2 cut(s) 315, 402
MluCI AATT 3 cut(s) 216, 416, 834
MmeI TCCRAC 1 cut(s) 484
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 1 cut(s) 306
MspI CCGG 6 cut(s) 123, 482, 497, 521, 726, 761
MvaI CCWGG 9 cut(s) 101, 155, 178, 290, 582, 642, 669, 696, 711
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 7 cut(s) 264, 270, 338, 443, 631, 634, 757
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 727
NdeII GATC 1 cut(s) 12
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 2 cut(s) 193, 349
NlaIV GGNNCC 7 cut(s) 14, 166, 167, 620, 653, 680, 707
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 401
PasI CCCWGGG 2 cut(s) 154, 710
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 204, 291, 300, 461
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 320
PflMI CCANNNNNTGG 4 cut(s) 523, 647, 674, 701
PpuMI RGGWCCY 1 cut(s) 165
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 315, 402
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 9 cut(s) 99, 153, 176, 288, 580, 640, 667, 694, 709
PspFI CCCAGC 4 cut(s) 566, 595, 756, 793
PspGI CCWGG 9 cut(s) 99, 153, 176, 288, 580, 640, 667, 694, 709
PspN4I GGNNCC 7 cut(s) 14, 166, 167, 620, 653, 680, 707
PspPI GGNCC 4 cut(s) 165, 506, 569, 713
PspPPI RGGWCCY 1 cut(s) 165
PspXI VCTCGAGB 1 cut(s) 401
PsuI RGATCY 1 cut(s) 12
RsaI GTAC 6 cut(s) 212, 593, 620, 653, 680, 707
RsaNI GTAC 6 cut(s) 211, 592, 619, 652, 679, 706
SacI GAGCTC 2 cut(s) 315, 402
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 4 cut(s) 165, 506, 569, 713
SduI GDGCHC 2 cut(s) 315, 402
SfaNI GCATC 4 cut(s) 149, 240, 735, 819
Sfr274I CTCGAG 1 cut(s) 401
SinI GGWCC 2 cut(s) 165, 506
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 216, 416, 834
SspMI CTAG 2 cut(s) 17, 821
SstI GAGCTC 2 cut(s) 315, 402
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 3 cut(s) 57, 81, 114
TaqI TCGA 3 cut(s) 24, 204, 402
TasI AATT 3 cut(s) 216, 416, 834
TauI GCSGC 8 cut(s) 341, 437, 461, 628, 631, 634, 637, 718
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 3 cut(s) 264, 558, 748
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 206, 362
TspGWI ACGGA 1 cut(s) 313
Van91I CCANNNNNTGG 4 cut(s) 523, 647, 674, 701
VpaK11BI GGWCC 2 cut(s) 165, 506
XapI RAATTY 1 cut(s) 416
XhoI CTCGAG 1 cut(s) 401
XspI CTAG 2 cut(s) 17, 821
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.