Rh4BG239100

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0009797
Physical Location & Seq
Forward (+)
40544022 .. 40545853
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG239100.1

Sequence Viewer

Length: 888 bp
ATGGAGCAAAGGACTCTGGAGCTGGAGCTGGTCTCCGCCAAGGACCTGAAGGACGTGAACCTCATCTCCAAGATGGACGTCTACGCCGTCGTCTCCCTCGACGGCGACGCCTACTACGGCAAGCAGAAGACCAAGACCAAAGTCGTCCGCTCCTGCGGCACCAACCCCACCTTCAATTACCCCATGAGGTTCACCGTCGACGATACTCTCACCCAACAGAACCGCCTCTCCCTCCAAGTCAAGCTCGTCTGCGAGCGCAGCCTCGGCGACAAGGACATCGGCGTCGTTTACGTCCCCGTCAAGGAGCTCTTCGACTCCTCCGACCCCAAGAACATGAAGTTCATATCGTACCAGGTCCGACGACCCTCCGGTAAGCCCAAGGGCGAGCTCAGCTTTTCTTATAAATTTGGGGAGAAGGTCACCGCGCCGGTGCCGGAGGTGGCCAAGAAAGGGGAGCCGGTGACTGCTTATCCGGTTGGAGGACCGTCCGCGCCGGCGTACGGGGCGTACCCACTTCAGGCGACGAGTTCGTCCGGGTCGGGTTACCCGCCGCCTCAACCCGGGTATGGCTACCCGCCGCCGCCGCCTGGGGCTGGATATCCGGAAGCGCCTCCACCGGGTTACGGGTACCCACCGCAGCAACCGGGCTACGCTTACCCGCCTCAGCAGCCCGGGTACGGGTACCCACCGCCGCCGCCGGGTTACGGGTACCCGCCGGTGCAGCAAGTGCAGCAACCGGCAAAGAAGAAGAGCAAGTTGGGAATGGGATTGGGCGCTGGGTTGCTAGGAGGCGCCCTTGGTGGGTTGTTGATTGGAGATATGATTTCCGATGGAGGCGGTGGATGCGGTGGCGGCGGCTGCGGCGGCGGCGGTTGCGGTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

295

Amino Acids

30.91

Weight (kDa)

8.85

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 109 6.9e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AasI GACNNNNNNGTC 2 cut(s) 281, 529
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 3 cut(s) 627, 681, 708
AccB1I GGYRCC 6 cut(s) 158, 430, 627, 681, 708, 791
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 81, 198
AccII CGCG 2 cut(s) 425, 491
AccIII TCCGGA 1 cut(s) 601
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 68, 500
AcyI GRCGYC 4 cut(s) 78, 108, 282, 792
AfaI GTAC 7 cut(s) 350, 500, 509, 629, 677, 683, 710
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 351, 586
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 740, 772
AloI GAACNNNNNNTCC 4 cut(s) 50, 82, 212, 244
AluBI AGCT 6 cut(s) 22, 28, 244, 307, 388, 393
AluI AGCT 6 cut(s) 22, 28, 244, 307, 388, 393
Alw21I GWGCWC 2 cut(s) 309, 390
Alw26I GTCTC 2 cut(s) 37, 97
Ama87I CYCGRG 2 cut(s) 560, 671
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 6 cut(s) 258, 637, 667, 721, 730, 858
ApoI RAATTY 1 cut(s) 404
Asp718I GGTACC 3 cut(s) 627, 681, 708
AspLEI GCGC 6 cut(s) 258, 427, 493, 610, 776, 794
AspS9I GGNCC 3 cut(s) 43, 355, 482
AsuC2I CCSGG 8 cut(s) 535, 561, 562, 618, 645, 672, 673, 699
AsuHPI GGTGA 4 cut(s) 184, 202, 412, 472
AvaI CYCGRG 2 cut(s) 560, 671
AvaII GGWCC 3 cut(s) 43, 355, 482
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 6 cut(s) 158, 430, 627, 681, 708, 791
BanII GRGCYC 2 cut(s) 309, 390
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 309, 390
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 6 cut(s) 270, 649, 679, 733, 742, 845
BccI CCATC 2 cut(s) 67, 824
BceAI ACGGC 3 cut(s) 71, 118, 133
BciT130I CCWGG 2 cut(s) 353, 588
BcnI CCSGG 8 cut(s) 535, 561, 562, 618, 645, 672, 673, 699
BcoDI GTCTC 2 cut(s) 37, 97
BfaI CTAG 1 cut(s) 785
BfoI RGCGCY 3 cut(s) 611, 777, 795
BlpI GCTNAGC 1 cut(s) 389
Bme18I GGWCC 3 cut(s) 43, 355, 482
BmeT110I CYCGRG 2 cut(s) 560, 671
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 43, 355, 482
BmiI GGNNCC 7 cut(s) 160, 432, 456, 629, 683, 710, 793
BmsI GCATC 1 cut(s) 833
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 2 cut(s) 38, 44
Bpu10I CCTNAGC 1 cut(s) 663
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 8 cut(s) 535, 561, 562, 618, 645, 672, 673, 699
BsaHI GRCGYC 4 cut(s) 78, 108, 282, 792
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 7 cut(s) 39, 262, 378, 560, 587, 671, 796
BsaWI WCCGGW 3 cut(s) 368, 472, 601
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 212, 242
Bse118I RCCGGY 5 cut(s) 427, 457, 493, 715, 736
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 2 cut(s) 353, 588
BseDI CCNNGG 7 cut(s) 39, 262, 378, 560, 587, 671, 796
BseGI GGATG 1 cut(s) 848
BseMII CTCAG 2 cut(s) 403, 677
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 6 cut(s) 270, 649, 679, 733, 742, 845
BseYI CCCAGC 1 cut(s) 776
BsgI GTGCAG 2 cut(s) 740, 749
Bsh1236I CGCG 2 cut(s) 425, 491
BshFI GGCC 1 cut(s) 443
BshNI GGYRCC 6 cut(s) 158, 430, 627, 681, 708, 791
BsiHKAI GWGCWC 2 cut(s) 309, 390
BsiHKCI CYCGRG 2 cut(s) 560, 671
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 37, 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 443
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 560, 671
Bsp1286I GDGCHC 2 cut(s) 309, 390
Bsp13I TCCGGA 1 cut(s) 601
Bsp1720I GCTNAGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 2 cut(s) 402, 676
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 2 cut(s) 425, 491
BspLI GGNNCC 7 cut(s) 160, 432, 456, 629, 683, 710, 793
BspQI GCTCTTC 2 cut(s) 314, 743
BspT107I GGYRCC 6 cut(s) 158, 430, 627, 681, 708, 791
BspTNI GGTCTC 1 cut(s) 37
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 5 cut(s) 427, 457, 493, 715, 736
BssAI RCCGGY 5 cut(s) 427, 457, 493, 715, 736
BssECI CCNNGG 7 cut(s) 39, 262, 378, 560, 587, 671, 796
BssNI GRCGYC 4 cut(s) 78, 108, 282, 792
BssT1I CCWWGG 3 cut(s) 39, 378, 796
Bst2UI CCWGG 2 cut(s) 353, 588
Bst4CI ACNGT 2 cut(s) 196, 486
Bst6I CTCTTC 2 cut(s) 314, 743
BstACI GRCGYC 4 cut(s) 78, 108, 282, 792
BstAPI GCANNNNNTGC 1 cut(s) 727
BstC8I GCNNGC 4 cut(s) 122, 254, 386, 495
BstDEI CTNAG 2 cut(s) 389, 663
BstEII GGTNACC 2 cut(s) 418, 542
BstF5I GGATG 1 cut(s) 848
BstFNI CGCG 2 cut(s) 425, 491
BstH2I RGCGCY 3 cut(s) 611, 777, 795
BstHHI GCGC 6 cut(s) 258, 427, 493, 610, 776, 794
BstMAI GTCTC 2 cut(s) 37, 97
BstNI CCWGG 2 cut(s) 353, 588
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 2 cut(s) 418, 542
BstUI CGCG 2 cut(s) 425, 491
BstV1I GCAGC 6 cut(s) 270, 649, 679, 733, 742, 845
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 443
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 1 cut(s) 848
Cac8I GCNNGC 4 cut(s) 122, 254, 386, 495
CfoI GCGC 6 cut(s) 258, 427, 493, 610, 776, 794
Cfr10I RCCGGY 5 cut(s) 427, 457, 493, 715, 736
Cfr13I GGNCC 3 cut(s) 43, 355, 482
Cfr9I CCCGGG 2 cut(s) 560, 671
CseI GACGC 2 cut(s) 116, 271
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 7 cut(s) 349, 499, 508, 628, 676, 682, 709
CviAII CATG 2 cut(s) 184, 334
CviQI GTAC 7 cut(s) 349, 499, 508, 628, 676, 682, 709
DdeI CTNAG 2 cut(s) 389, 663
DinI GGCGCC 1 cut(s) 793
DrdI GACNNNNNNGTC 2 cut(s) 281, 529
DseDI GACNNNNNNGTC 2 cut(s) 281, 529
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 314, 743
EarI CTCTTC 2 cut(s) 314, 743
EciI GGCGGA 1 cut(s) 25
Ecl136II GAGCTC 2 cut(s) 307, 388
Eco130I CCWWGG 3 cut(s) 39, 378, 796
Eco24I GRGCYC 2 cut(s) 309, 390
Eco31I GGTCTC 1 cut(s) 37
Eco32I GATATC 1 cut(s) 599
Eco47I GGWCC 3 cut(s) 43, 355, 482
Eco53kI GAGCTC 2 cut(s) 307, 388
Eco57I CTGAAG 2 cut(s) 68, 500
Eco88I CYCGRG 2 cut(s) 560, 671
Eco91I GGTNACC 2 cut(s) 418, 542
EcoICRI GAGCTC 2 cut(s) 307, 388
EcoO109I RGGNCCY 1 cut(s) 43
EcoO65I GGTNACC 2 cut(s) 418, 542
EcoRII CCWGG 2 cut(s) 351, 586
EcoRV GATATC 1 cut(s) 599
EcoT14I CCWWGG 3 cut(s) 39, 378, 796
EcoT38I GRGCYC 2 cut(s) 309, 390
EgeI GGCGCC 1 cut(s) 793
EheI GGCGCC 1 cut(s) 793
ErhI CCWWGG 3 cut(s) 39, 378, 796
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 187, 337
FaiI YATR 6 cut(s) 185, 335, 344, 402, 567, 821
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 183, 333
FauI CCCGC 4 cut(s) 555, 582, 666, 720
FblI GTMKAC 2 cut(s) 81, 198
FokI GGATG 1 cut(s) 855
FriOI GRGCYC 2 cut(s) 309, 390
FspBI CTAG 1 cut(s) 785
GlaI GCGC 6 cut(s) 257, 426, 492, 609, 775, 793
GsaI CCCAGC 1 cut(s) 780
GsuI CTGGAG 2 cut(s) 38, 44
HaeII RGCGCY 3 cut(s) 611, 777, 795
HaeIII GGCC 1 cut(s) 443
HgaI GACGC 2 cut(s) 116, 271
HhaI GCGC 6 cut(s) 258, 427, 493, 610, 776, 794
Hin1I GRCGYC 4 cut(s) 78, 108, 282, 792
Hin1II CATG 2 cut(s) 187, 337
Hin6I GCGC 6 cut(s) 256, 425, 491, 608, 774, 792
HinP1I GCGC 6 cut(s) 256, 425, 491, 608, 774, 792
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 2 cut(s) 13, 314
HphI GGTGA 4 cut(s) 184, 202, 412, 472
Hpy166II GTNNAC 5 cut(s) 58, 82, 192, 199, 289
Hpy188I TCNGA 3 cut(s) 322, 359, 829
Hpy188III TCNNGA 2 cut(s) 17, 602
Hpy8I GTNNAC 5 cut(s) 58, 82, 192, 199, 289
Hpy99I CGWCG 8 cut(s) 92, 104, 110, 200, 203, 287, 363, 526
HpyAV CCTTC 3 cut(s) 43, 181, 409
HpyCH4III ACNGT 2 cut(s) 196, 486
HpyCH4IV ACGT 3 cut(s) 54, 78, 291
HpyCH4V TGCA 2 cut(s) 721, 730
HpyF3I CTNAG 2 cut(s) 389, 663
HpySE526I ACGT 3 cut(s) 54, 78, 291
Hsp92I GRCGYC 4 cut(s) 78, 108, 282, 792
Hsp92II CATG 2 cut(s) 187, 337
HspAI GCGC 6 cut(s) 256, 425, 491, 608, 774, 792
KasI GGCGCC 1 cut(s) 791
Kpn2I TCCGGA 1 cut(s) 601
KpnI GGTACC 3 cut(s) 631, 685, 712
KroI GCCGGC 1 cut(s) 493
KroNI GCCGGC 1 cut(s) 495
LguI GCTCTTC 2 cut(s) 314, 743
LmnI GCTCC 6 cut(s) 4, 19, 25, 155, 304, 454
Lsp1109I GCAGC 6 cut(s) 270, 649, 679, 733, 742, 845
LweI GCATC 1 cut(s) 833
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 1 cut(s) 785
MaeII ACGT 3 cut(s) 54, 78, 291
MaeIII GTNAC 5 cut(s) 418, 460, 542, 620, 701
MbiI CCGCTC 1 cut(s) 150
MboII GAAGA 4 cut(s) 139, 301, 757, 760
MhlI GDGCHC 2 cut(s) 309, 390
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 2 cut(s) 175, 404
MluNI TGGCCA 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 792
MlyI GAGTC 2 cut(s) 7, 308
MmeI TCCRAC 3 cut(s) 345, 382, 457
Mox20I TGGCCA 1 cut(s) 443
MreI CGCCGGCG 1 cut(s) 493
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 493
MscI TGGCCA 1 cut(s) 443
Msp20I TGGCCA 1 cut(s) 443
MvaI CCWGG 2 cut(s) 353, 588
MvnI CGCG 2 cut(s) 425, 491
NaeI GCCGGC 1 cut(s) 495
NarI GGCGCC 1 cut(s) 792
NciI CCSGG 8 cut(s) 535, 561, 562, 618, 645, 672, 673, 699
NgoMIV GCCGGC 1 cut(s) 493
NlaIII CATG 2 cut(s) 187, 337
NlaIV GGNNCC 7 cut(s) 160, 432, 456, 629, 683, 710, 793
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 2 cut(s) 418, 460
PciSI GCTCTTC 2 cut(s) 314, 743
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 96, 318
PdiI GCCGGC 1 cut(s) 495
Pfl23II CGTACG 1 cut(s) 498
PleI GAGTC 2 cut(s) 7, 308
PluTI GGCGCC 1 cut(s) 795
PpsI GAGTC 2 cut(s) 7, 308
PpuMI RGGWCCY 1 cut(s) 43
PshAI GACNNNNGTC 1 cut(s) 140
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 309, 390
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 351, 586
PspEI GGTNACC 2 cut(s) 418, 542
PspFI CCCAGC 1 cut(s) 776
PspGI CCWGG 2 cut(s) 351, 586
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 7 cut(s) 160, 432, 456, 629, 683, 710, 793
PspPI GGNCC 3 cut(s) 43, 355, 482
PspPPI RGGWCCY 1 cut(s) 43
RsaI GTAC 7 cut(s) 350, 500, 509, 629, 677, 683, 710
RsaNI GTAC 7 cut(s) 349, 499, 508, 628, 676, 682, 709
SacI GAGCTC 2 cut(s) 309, 390
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 2 cut(s) 314, 743
Sau96I GGNCC 3 cut(s) 43, 355, 482
SchI GAGTC 2 cut(s) 7, 308
SduI GDGCHC 2 cut(s) 309, 390
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 1 cut(s) 833
SfoI GGCGCC 1 cut(s) 793
SgrAI CRCCGGYG 3 cut(s) 427, 493, 715
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 3 cut(s) 43, 355, 482
SmaI CCCGGG 2 cut(s) 562, 673
Sse9I AATT 2 cut(s) 175, 404
SspDI GGCGCC 1 cut(s) 791
SspMI CTAG 1 cut(s) 785
SstI GAGCTC 2 cut(s) 309, 390
StyI CCWWGG 3 cut(s) 39, 378, 796
TaaI ACNGT 2 cut(s) 196, 486
TaiI ACGT 3 cut(s) 57, 81, 294
TaqI TCGA 3 cut(s) 99, 198, 312
TasI AATT 2 cut(s) 175, 404
TseFI GTSAC 2 cut(s) 418, 460
TseI GCWGC 6 cut(s) 258, 637, 667, 721, 730, 858
Tsp45I GTSAC 2 cut(s) 418, 460
TspDTI ATGAA 2 cut(s) 331, 350
TspMI CCCGGG 2 cut(s) 560, 671
VpaK11BI GGWCC 3 cut(s) 43, 355, 482
XapI RAATTY 1 cut(s) 404
XmaI CCCGGG 2 cut(s) 560, 671
XmiI GTMKAC 2 cut(s) 81, 198
XspI CTAG 1 cut(s) 785
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.