pycom16g14350

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009797
Physical Location & Seq
Reverse (-)
10398281 .. 10399165
885 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g14350.1

Sequence Viewer

Length: 885 bp
ATGGATCAGAGGACCCTAGAGCTCGAACTCATCTCAGCCAAAGACATCAAAGACGTCAACCTCATATCCAAGATGGACGTATACGCCGTCGTCTCCCTCCAAGGCGACGACGACTCTCGCGGCAAGCAGCAGAAAACCAAGACCGAAGTCGCCCGGGGATGCGGGACCCATCCCACCTGGAACTTCCACACGAAGTTCGTTCTCGACGAGTTCCTAACTCAGCAGAACCGCCTCTTCATTGTCTTCAAGCTCGTCTGCCAGCGCAGCCTCGGCGACAAAGACATCGGCCAGGTCGTCGTCCCCGTTAAGGAGCTTTTTGACTCCGTCAAGGCTGACGCCGCCTCCATGAAGTTCGTCGCTTACCAAGTCAGAAAGCCCTCTGGGAAGCCGAAAGGTGAGCTCAATTTTTCCTACAAATTTGGTGACAAAGTGGCGGCTCCACAGGCAGCGGCGACTGCGGTACCATCGAAGGGTAACGAGCCGGTAACAGCCTATCCGGTCGTTGGACCGTCAGTCCAGCCGATGGTGGGGTACGGTGGATACCCCCCTCCAGTTGGCACCTCTGCTGGGCCTCCGCCACCTGGATACGAGTACCCACCACCGCCGCCTGGATATGGATACCCGCCACCGCCACCGGGATACGGCTACCCGCCACCGCCACCAGGGTATGGGTACCCGGGGCCGCCACCTCCGGGGTATGGGTACCCCGCGGTGCAGCAAGCCCAACCGGAAGCAAAGAAGAAGAACAAGTTTGGGATGGGACTGGGGGCTGGATTGTTAGGGGGAATGCTTGGTGGTTTGTTGATTGGAGATATGGTATCTGGTGGCGGTGGCTGCGGTGGCGGTGGCGGTGGCTGCGGCGGTGGCGGTGGATGTGGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

295

Amino Acids

30.45

Weight (kDa)

8.78

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 109 9.6e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 3 cut(s) 460, 672, 702
AccB1I GGYRCC 4 cut(s) 460, 557, 672, 702
AccB7I CCANNNNNTGG 2 cut(s) 523, 668
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 120, 710
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 416
AcyI GRCGYC 2 cut(s) 54, 336
AfaI GTAC 5 cut(s) 462, 533, 593, 674, 704
AgsI TTSAA 1 cut(s) 247
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 5 cut(s) 176, 288, 580, 607, 661
AluBI AGCT 4 cut(s) 22, 250, 313, 400
AluI AGCT 4 cut(s) 22, 250, 313, 400
Alw21I GWGCWC 2 cut(s) 24, 402
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 2 cut(s) 153, 676
AoxI GGCC 3 cut(s) 286, 569, 680
ApeKI GCWGC 6 cut(s) 127, 264, 446, 715, 834, 855
ApoI RAATTY 1 cut(s) 416
Asp718I GGTACC 3 cut(s) 460, 672, 702
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 5 cut(s) 12, 165, 506, 569, 680
AsuC2I CCSGG 6 cut(s) 154, 155, 636, 677, 678, 693
AsuHPI GGTGA 2 cut(s) 407, 434
AvaI CYCGRG 2 cut(s) 153, 676
AvaII GGWCC 3 cut(s) 12, 165, 506
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BanI GGYRCC 4 cut(s) 460, 557, 672, 702
BanII GRGCYC 2 cut(s) 24, 402
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 24, 402
BbvI GCAGC 6 cut(s) 139, 276, 458, 727, 821, 842
BccI CCATC 5 cut(s) 67, 177, 472, 517, 751
BceAI ACGGC 2 cut(s) 71, 658
BciT130I CCWGG 5 cut(s) 178, 290, 582, 609, 663
BciVI GTATCC 4 cut(s) 533, 578, 611, 632
BcnI CCSGG 6 cut(s) 154, 155, 636, 677, 678, 693
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 17
BfuI GTATCC 4 cut(s) 533, 578, 611, 632
Bme18I GGWCC 3 cut(s) 12, 165, 506
BmeRI GACNNNNNGTC 1 cut(s) 512
BmeT110I CYCGRG 2 cut(s) 153, 676
BmgT120I GGNCC 5 cut(s) 12, 165, 506, 569, 680
BmiI GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 674, 681, 704
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 773
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 1 cut(s) 534
BpuMI CCSGG 6 cut(s) 154, 155, 636, 677, 678, 693
BsaHI GRCGYC 2 cut(s) 54, 336
BsaJI CCNNGG 9 cut(s) 100, 153, 154, 268, 662, 676, 677, 692, 708
BsaWI WCCGGW 2 cut(s) 496, 727
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 801, 831
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 2 cut(s) 551, 768
BseBI CCWGG 5 cut(s) 178, 290, 582, 609, 663
BseDI CCNNGG 9 cut(s) 100, 153, 154, 268, 662, 676, 677, 692, 708
BseGI GGATG 4 cut(s) 164, 169, 762, 878
BseMII CTCAG 2 cut(s) 48, 233
BseNI ACTGG 2 cut(s) 551, 768
BseXI GCAGC 6 cut(s) 139, 276, 458, 727, 821, 842
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 2 cut(s) 120, 710
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 3 cut(s) 288, 571, 682
BshNI GGYRCC 4 cut(s) 460, 557, 672, 702
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 24, 402
BsiHKCI CYCGRG 2 cut(s) 153, 676
BsiSI CCGG 7 cut(s) 154, 482, 497, 635, 677, 692, 728
BslFI GGGAC 3 cut(s) 178, 284, 774
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 3 cut(s) 178, 284, 774
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 3 cut(s) 288, 571, 682
BsoBI CYCGRG 2 cut(s) 153, 676
Bsp1286I GDGCHC 2 cut(s) 24, 402
Bsp143I GATC 1 cut(s) 4
BspANI GGCC 3 cut(s) 288, 571, 682
BspCNI CTCAG 2 cut(s) 47, 232
BspFNI CGCG 2 cut(s) 120, 710
BspLI GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 674, 681, 704
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 4 cut(s) 460, 557, 672, 702
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 2 cut(s) 551, 768
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 9 cut(s) 100, 153, 154, 268, 662, 676, 677, 692, 708
BssMI GATC 1 cut(s) 4
BssNAI GTATAC 1 cut(s) 82
BssNI GRCGYC 2 cut(s) 54, 336
BssT1I CCWWGG 1 cut(s) 100
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 5 cut(s) 178, 290, 582, 609, 663
Bst4CI ACNGT 2 cut(s) 510, 536
Bst6I CTCTTC 1 cut(s) 239
BstACI GRCGYC 2 cut(s) 54, 336
BstC8I GCNNGC 3 cut(s) 125, 260, 720
BstDEI CTNAG 2 cut(s) 34, 219
BstDSI CCRYGG 1 cut(s) 708
BstF5I GGATG 4 cut(s) 164, 169, 762, 878
BstFNI CGCG 2 cut(s) 120, 710
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 1 cut(s) 4
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 9 cut(s) 264, 270, 338, 443, 455, 834, 840, 855, 864
BstNI CCWGG 5 cut(s) 178, 290, 582, 609, 663
BstPAI GACNNNNGTC 1 cut(s) 146
BstUI CGCG 2 cut(s) 120, 710
BstV1I GCAGC 6 cut(s) 139, 276, 458, 727, 821, 842
BstV2I GAAGAC 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 82
BsuI GTATCC 4 cut(s) 533, 578, 611, 632
BsuRI GGCC 3 cut(s) 288, 571, 682
BtgI CCRYGG 1 cut(s) 708
BtsCI GGATG 4 cut(s) 164, 169, 762, 878
Cac8I GCNNGC 3 cut(s) 125, 260, 720
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 5 cut(s) 12, 165, 506, 569, 680
Cfr42I CCGCGG 1 cut(s) 711
Cfr9I CCCGGG 2 cut(s) 153, 676
CseI GACGC 1 cut(s) 344
Csp6I GTAC 5 cut(s) 461, 532, 592, 673, 703
CviAII CATG 1 cut(s) 346
CviQI GTAC 5 cut(s) 461, 532, 592, 673, 703
DdeI CTNAG 2 cut(s) 34, 219
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 239
Eam1105I GACNNNNNGTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 22, 400
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 2 cut(s) 24, 402
Eco47I GGWCC 3 cut(s) 12, 165, 506
Eco53kI GAGCTC 2 cut(s) 22, 400
Eco88I CYCGRG 2 cut(s) 153, 676
EcoICRI GAGCTC 2 cut(s) 22, 400
EcoO109I RGGNCCY 2 cut(s) 12, 165
EcoRII CCWGG 5 cut(s) 176, 288, 580, 607, 661
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 2 cut(s) 24, 402
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 1 cut(s) 349
FaiI YATR 7 cut(s) 65, 82, 347, 615, 669, 699, 815
FaqI GGGAC 3 cut(s) 178, 284, 774
FatI CATG 1 cut(s) 345
FauI CCCGC 4 cut(s) 155, 630, 657, 715
FblI GTMKAC 1 cut(s) 81
FokI GGATG 3 cut(s) 156, 171, 769
FriOI GRGCYC 2 cut(s) 24, 402
FspBI CTAG 1 cut(s) 17
GlaI GCGC 1 cut(s) 263
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 288, 571, 682
HapII CCGG 7 cut(s) 154, 482, 497, 635, 677, 692, 728
HgaI GACGC 1 cut(s) 344
HhaI GCGC 1 cut(s) 264
Hin1I GRCGYC 2 cut(s) 54, 336
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 2 cut(s) 113, 320
HpaII CCGG 7 cut(s) 154, 482, 497, 635, 677, 692, 728
HphI GGTGA 2 cut(s) 407, 434
Hpy166II GTNNAC 2 cut(s) 58, 82
Hpy188I TCNGA 2 cut(s) 9, 371
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 2 cut(s) 58, 82
Hpy99I CGWCG 6 cut(s) 92, 110, 113, 209, 299, 359
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 510, 536
HpyCH4IV ACGT 2 cut(s) 54, 78
HpyCH4V TGCA 1 cut(s) 715
HpyF10VI GCNNNNNNNGC 9 cut(s) 264, 270, 338, 443, 455, 834, 840, 855, 864
HpyF3I CTNAG 2 cut(s) 34, 219
HpySE526I ACGT 2 cut(s) 54, 78
Hsp92I GRCGYC 2 cut(s) 54, 336
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 1 cut(s) 262
KflI GGGWCCC 1 cut(s) 165
KpnI GGTACC 3 cut(s) 464, 676, 706
KspI CCGCGG 1 cut(s) 711
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 2 cut(s) 310, 442
Lsp1109I GCAGC 6 cut(s) 139, 276, 458, 727, 821, 842
LweI GCATC 1 cut(s) 149
MaeI CTAG 1 cut(s) 17
MaeII ACGT 2 cut(s) 54, 78
MaeIII GTNAC 3 cut(s) 422, 473, 484
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 4 cut(s) 226, 235, 751, 754
MhlI GDGCHC 2 cut(s) 24, 402
MluCI AATT 2 cut(s) 403, 416
MlyI GAGTC 2 cut(s) 107, 314
MmeI TCCRAC 1 cut(s) 484
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 2 cut(s) 449, 710
MspI CCGG 7 cut(s) 154, 482, 497, 635, 677, 692, 728
Mva1269I GAATGC 1 cut(s) 792
MvaI CCWGG 5 cut(s) 178, 290, 582, 609, 663
MvnI CGCG 2 cut(s) 120, 710
MwoI GCNNNNNNNGC 9 cut(s) 264, 270, 338, 443, 455, 834, 840, 855, 864
NciI CCSGG 6 cut(s) 154, 155, 636, 677, 678, 693
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 674, 681, 704
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 204, 291, 300
PctI GAATGC 1 cut(s) 792
PflFI GACNNNGTC 1 cut(s) 323
PflMI CCANNNNNTGG 2 cut(s) 523, 668
PleI GAGTC 2 cut(s) 107, 314
PpsI GAGTC 2 cut(s) 107, 314
PpuMI RGGWCCY 2 cut(s) 12, 165
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 24, 402
Psp5II RGGWCCY 2 cut(s) 12, 165
Psp6I CCWGG 5 cut(s) 176, 288, 580, 607, 661
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 5 cut(s) 176, 288, 580, 607, 661
PspN4I GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 674, 681, 704
PspPI GGNCC 5 cut(s) 12, 165, 506, 569, 680
PspPPI RGGWCCY 2 cut(s) 12, 165
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 5 cut(s) 462, 533, 593, 674, 704
RsaNI GTAC 5 cut(s) 461, 532, 592, 673, 703
SacI GAGCTC 2 cut(s) 24, 402
SacII CCGCGG 1 cut(s) 711
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 5 cut(s) 12, 165, 506, 569, 680
SchI GAGTC 2 cut(s) 107, 314
SduI GDGCHC 2 cut(s) 24, 402
SfaNI GCATC 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 711
SgrBI CCGCGG 1 cut(s) 711
SinI GGWCC 3 cut(s) 12, 165, 506
SmaI CCCGGG 2 cut(s) 155, 678
Sse9I AATT 2 cut(s) 403, 416
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 2 cut(s) 24, 402
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 2 cut(s) 510, 536
TaiI ACGT 2 cut(s) 57, 81
TaqI TCGA 3 cut(s) 24, 204, 467
TaqII GACCGA 1 cut(s) 158
TasI AATT 2 cut(s) 403, 416
TauI GCSGC 7 cut(s) 123, 341, 437, 452, 607, 685, 861
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 6 cut(s) 127, 264, 446, 715, 834, 855
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 226, 362
TspGWI ACGGA 1 cut(s) 313
TspMI CCCGGG 2 cut(s) 153, 676
Tth111I GACNNNGTC 1 cut(s) 323
Van91I CCANNNNNTGG 2 cut(s) 523, 668
VpaK11BI GGWCC 3 cut(s) 12, 165, 506
XapI RAATTY 1 cut(s) 416
XmaI CCCGGG 2 cut(s) 153, 676
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.