FvH4_4g17880

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009797
Physical Location & Seq
Forward (+)
21849491 .. 21850898
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17880.t1

Sequence Viewer

Length: 876 bp
ATGGAGCAAAGGACTCTGGAGCTGGAGCTGGTCTCCGCCAAGGACCTAAAAGATGTGAACCTCATCTCCAAGATGGACGTCTACGCCGTCGTCTCCCTCGACGGCGACGCCTACAACGGCAAGCAGAAGACCAAAACCAAACTCGTCCCAAACTGCGGCACCAACCCCACCTTCAACTTCCCCATGCGCTTCACCCTCGACGACTCTCTCACCCAACAGAACCGCCTCTCCCTCGAAGTCAAGCTCGTCTGCGAGCGCAGCCTCGGCGACAAGGACATCGGCGCCGTCCACGTCCCCGTCAAGGAGCTCTTCGACTCCGCCGACCCCAAGAACATGAAGTTCATTTCGTATCAGGTCCGGCGACCCTCCGGCAGGCCCAAGGGCGAGCTGAGTTTCTCTTACAAGTTTGGGGAAAAGGTGGCCGTGCCGGCGCCGGAAGTTGCGAAGAAAGGTGAGCCGGTGACGGCGTACCCGGTTGGTGCGCCGTCGTACGGGGCGTACGCGCAACAGGCCACGAGTTCGTCCGGGTCGGGTTACCCGCCGCCTCAACCCGGGTATGGATACCCGCCGCCGCCTCCTGGGGCGGGATACCCGGAAGCGCCTCCACCGGGATACGGGTACCCACCACCGCAACCGGGTTACGCGTACCCGCCGCAGCAGCCGGGGTATGGATATCCGCCGCCGCCGCCGGGATACGGATACCCGCCGGTGCAGCAGCAGCAACCGGAGAAGAAGAAGAACAAGTTTGGGATGGGATTGGGCGCAGGGTTGCTAGGAGGTGTGCTTGGTGGGATGTTGATTGGAGATATGGTGTCTGACGGCGGCGGTGGATGCGGTGGCGGAGGATGCGGCGGTGGCGGTTGTGGTGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

292

Amino Acids

30.54

Weight (kDa)

8.41

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 107 2.8e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 618
AccB1I GGYRCC 4 cut(s) 158, 281, 430, 618
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 503, 644
AcoI YGGCCR 1 cut(s) 420
AcyI GRCGYC 4 cut(s) 78, 108, 282, 431
AfaI GTAC 5 cut(s) 470, 491, 500, 620, 647
AflIII ACRYGT 1 cut(s) 642
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 577
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AloI GAACNNNNNNTCC 4 cut(s) 50, 82, 212, 244
AluBI AGCT 5 cut(s) 22, 28, 244, 307, 388
AluI AGCT 5 cut(s) 22, 28, 244, 307, 388
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 2 cut(s) 37, 97
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 3 cut(s) 374, 420, 510
ApeKI GCWGC 6 cut(s) 258, 655, 658, 712, 715, 718
Asp718I GGTACC 1 cut(s) 618
AspLEI GCGC 8 cut(s) 189, 258, 284, 433, 484, 505, 601, 764
AspS9I GGNCC 3 cut(s) 43, 355, 375
AsuC2I CCSGG 9 cut(s) 473, 526, 552, 553, 593, 609, 636, 663, 690
AsuHPI GGTGA 4 cut(s) 184, 202, 464, 472
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 43, 355
BanI GGYRCC 4 cut(s) 158, 281, 430, 618
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 6 cut(s) 270, 667, 670, 724, 727, 730
BccI CCATC 2 cut(s) 67, 745
BceAI ACGGC 8 cut(s) 71, 118, 133, 269, 407, 469, 480, 835
BciT130I CCWGG 1 cut(s) 579
BciVI GTATCC 5 cut(s) 554, 581, 605, 686, 692
BcnI CCSGG 9 cut(s) 473, 526, 552, 553, 593, 609, 636, 663, 690
BcoDI GTCTC 2 cut(s) 37, 97
BfaI CTAG 1 cut(s) 773
BfoI RGCGCY 3 cut(s) 285, 434, 602
BfuI GTATCC 5 cut(s) 554, 581, 605, 686, 692
BglI GCCNNNNNGGC 1 cut(s) 428
Bme18I GGWCC 2 cut(s) 43, 355
BmeT110I CYCGRG 1 cut(s) 551
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 43, 355, 375
BmiI GGNNCC 4 cut(s) 160, 283, 432, 620
BmsI GCATC 2 cut(s) 821, 836
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 2 cut(s) 38, 44
BpuMI CCSGG 9 cut(s) 473, 526, 552, 553, 593, 609, 636, 663, 690
BsaHI GRCGYC 4 cut(s) 78, 108, 282, 431
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 6 cut(s) 39, 262, 378, 551, 578, 662
BsaWI WCCGGW 1 cut(s) 724
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 212, 242, 795, 825
Bse118I RCCGGY 3 cut(s) 427, 457, 706
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 6 cut(s) 39, 262, 378, 551, 578, 662
BseGI GGATG 4 cut(s) 756, 798, 836, 851
BseMII CTCAG 1 cut(s) 380
BseXI GCAGC 6 cut(s) 270, 667, 670, 724, 727, 730
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 503, 644
BshFI GGCC 3 cut(s) 376, 422, 512
BshNI GGYRCC 4 cut(s) 158, 281, 430, 618
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 551
BsiWI CGTACG 2 cut(s) 489, 498
BslFI GGGAC 2 cut(s) 131, 278
BsmAI GTCTC 2 cut(s) 37, 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 131, 278
BsnI GGCC 3 cut(s) 376, 422, 512
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 309
BspANI GGCC 3 cut(s) 376, 422, 512
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 2 cut(s) 503, 644
BspLI GGNNCC 4 cut(s) 160, 283, 432, 620
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 4 cut(s) 158, 281, 430, 618
BspTNI GGTCTC 1 cut(s) 37
BsrFI RCCGGY 3 cut(s) 427, 457, 706
BssAI RCCGGY 3 cut(s) 427, 457, 706
BssECI CCNNGG 6 cut(s) 39, 262, 378, 551, 578, 662
BssNI GRCGYC 4 cut(s) 78, 108, 282, 431
BssSI CACGAG 1 cut(s) 514
BssT1I CCWWGG 2 cut(s) 39, 378
Bst2BI CACGAG 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 579
Bst6I CTCTTC 1 cut(s) 314
BstACI GRCGYC 4 cut(s) 78, 108, 282, 431
BstC8I GCNNGC 5 cut(s) 122, 254, 374, 386, 429
BstDEI CTNAG 1 cut(s) 389
BstEII GGTNACC 1 cut(s) 533
BstENI CCTNNNNNAGG 1 cut(s) 370
BstF5I GGATG 4 cut(s) 756, 798, 836, 851
BstFNI CGCG 2 cut(s) 503, 644
BstH2I RGCGCY 3 cut(s) 285, 434, 602
BstHHI GCGC 8 cut(s) 189, 258, 284, 433, 484, 505, 601, 764
BstMAI GTCTC 2 cut(s) 37, 97
BstNI CCWGG 1 cut(s) 579
BstPI GGTNACC 1 cut(s) 533
BstUI CGCG 2 cut(s) 503, 644
BstV1I GCAGC 6 cut(s) 270, 667, 670, 724, 727, 730
BstV2I GAAGAC 1 cut(s) 134
BsuI GTATCC 5 cut(s) 554, 581, 605, 686, 692
BsuRI GGCC 3 cut(s) 376, 422, 512
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 4 cut(s) 756, 798, 836, 851
Cac8I GCNNGC 5 cut(s) 122, 254, 374, 386, 429
CfoI GCGC 8 cut(s) 189, 258, 284, 433, 484, 505, 601, 764
Cfr10I RCCGGY 3 cut(s) 427, 457, 706
Cfr13I GGNCC 3 cut(s) 43, 355, 375
Cfr9I CCCGGG 1 cut(s) 551
CseI GACGC 1 cut(s) 116
Csp6I GTAC 5 cut(s) 469, 490, 499, 619, 646
CviAII CATG 2 cut(s) 184, 334
CviQI GTAC 5 cut(s) 469, 490, 499, 619, 646
DdeI CTNAG 1 cut(s) 389
DinI GGCGCC 2 cut(s) 283, 432
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
EciI GGCGGA 4 cut(s) 25, 307, 666, 855
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 39, 378
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 37
Eco32I GATATC 1 cut(s) 674
Eco47I GGWCC 2 cut(s) 43, 355
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 551
Eco91I GGTNACC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 307
EcoNI CCTNNNNNAGG 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 577
EcoRV GATATC 1 cut(s) 674
EcoT14I CCWWGG 2 cut(s) 39, 378
EcoT38I GRGCYC 1 cut(s) 309
EgeI GGCGCC 2 cut(s) 283, 432
EheI GGCGCC 2 cut(s) 283, 432
ErhI CCWWGG 2 cut(s) 39, 378
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 187, 337
FaiI YATR 5 cut(s) 185, 335, 558, 669, 809
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 2 cut(s) 131, 278
FatI CATG 2 cut(s) 183, 333
FauI CCCGC 5 cut(s) 546, 573, 577, 657, 711
FblI GTMKAC 1 cut(s) 81
FokI GGATG 4 cut(s) 763, 805, 843, 858
FriOI GRGCYC 1 cut(s) 309
FspBI CTAG 1 cut(s) 773
GlaI GCGC 8 cut(s) 188, 257, 283, 432, 483, 504, 600, 763
GsuI CTGGAG 2 cut(s) 38, 44
HaeII RGCGCY 3 cut(s) 285, 434, 602
HaeIII GGCC 3 cut(s) 376, 422, 512
HgaI GACGC 1 cut(s) 116
HhaI GCGC 8 cut(s) 189, 258, 284, 433, 484, 505, 601, 764
Hin1I GRCGYC 4 cut(s) 78, 108, 282, 431
Hin1II CATG 2 cut(s) 187, 337
Hin6I GCGC 8 cut(s) 187, 256, 282, 431, 482, 503, 599, 762
HinP1I GCGC 8 cut(s) 187, 256, 282, 431, 482, 503, 599, 762
HinfI GANTC 3 cut(s) 13, 203, 314
HphI GGTGA 4 cut(s) 184, 202, 464, 472
Hpy166II GTNNAC 3 cut(s) 58, 82, 289
Hpy188I TCNGA 1 cut(s) 817
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 3 cut(s) 58, 82, 289
Hpy99I CGWCG 5 cut(s) 92, 104, 110, 203, 490
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 2 cut(s) 78, 291
HpyCH4V TGCA 1 cut(s) 712
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 2 cut(s) 78, 291
Hsp92I GRCGYC 4 cut(s) 78, 108, 282, 431
Hsp92II CATG 2 cut(s) 187, 337
HspAI GCGC 8 cut(s) 187, 256, 282, 431, 482, 503, 599, 762
KasI GGCGCC 2 cut(s) 281, 430
KpnI GGTACC 1 cut(s) 622
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 4 cut(s) 4, 19, 25, 304
Lsp1109I GCAGC 6 cut(s) 270, 667, 670, 724, 727, 730
LweI GCATC 2 cut(s) 821, 836
MaeI CTAG 1 cut(s) 773
MaeII ACGT 2 cut(s) 78, 291
MaeIII GTNAC 3 cut(s) 460, 533, 638
MboII GAAGA 6 cut(s) 139, 301, 457, 742, 745, 748
MhlI GDGCHC 1 cut(s) 309
MluI ACGCGT 1 cut(s) 642
Mly113I GGCGCC 2 cut(s) 282, 431
MlyI GAGTC 3 cut(s) 7, 197, 308
MroNI GCCGGC 1 cut(s) 427
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 2 cut(s) 503, 644
NaeI GCCGGC 1 cut(s) 429
NarI GGCGCC 2 cut(s) 282, 431
NciI CCSGG 9 cut(s) 473, 526, 552, 553, 593, 609, 636, 663, 690
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 2 cut(s) 187, 337
NlaIV GGNNCC 4 cut(s) 160, 283, 432, 620
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 96, 318, 494
PdiI GCCGGC 1 cut(s) 429
Pfl23II CGTACG 2 cut(s) 489, 498
PleI GAGTC 3 cut(s) 7, 197, 308
PluTI GGCGCC 2 cut(s) 285, 434
PpsI GAGTC 3 cut(s) 7, 197, 308
PpuMI RGGWCCY 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 309
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 577
PspEI GGTNACC 1 cut(s) 533
PspGI CCWGG 1 cut(s) 577
PspLI CGTACG 2 cut(s) 489, 498
PspN4I GGNNCC 4 cut(s) 160, 283, 432, 620
PspPI GGNCC 3 cut(s) 43, 355, 375
PspPPI RGGWCCY 1 cut(s) 43
RsaI GTAC 5 cut(s) 470, 491, 500, 620, 647
RsaNI GTAC 5 cut(s) 469, 490, 499, 619, 646
SacI GAGCTC 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 314
Sau96I GGNCC 3 cut(s) 43, 355, 375
SchI GAGTC 3 cut(s) 7, 197, 308
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 821, 836
SfoI GGCGCC 2 cut(s) 283, 432
SgrAI CRCCGGYG 1 cut(s) 706
SinI GGWCC 2 cut(s) 43, 355
SmaI CCCGGG 1 cut(s) 553
SspDI GGCGCC 2 cut(s) 281, 430
SspMI CTAG 1 cut(s) 773
SstI GAGCTC 1 cut(s) 309
StyI CCWWGG 2 cut(s) 39, 378
TaiI ACGT 2 cut(s) 81, 294
TaqI TCGA 4 cut(s) 99, 198, 234, 312
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 6 cut(s) 258, 655, 658, 712, 715, 718
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 2 cut(s) 331, 350
TspGWI ACGGA 1 cut(s) 711
TspMI CCCGGG 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 43, 355
XagI CCTNNNNNAGG 1 cut(s) 370
XmaI CCCGGG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 773
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.