MD16G1170900.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009797
Physical Location & Seq
Reverse (-)
14319259 .. 14320164
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1170900.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGAGCAGAGGACCCTGGAGCTCGAACTCATCTCAGCCAAGGACATCAAAGACGTCAACCTCATGTCCAAGATGGACGTATACGCCGTCGTCTCCCTCCAAGGCGACGACGACTCTGGCGGCAAGCAGCAGAAAACCAAGACCAAAGTCGTCCGGGGATGCGGGACCCATCCCACCTGGAACTTCCACACGAAGTTCGTTCTCGACGAGTACCTAACTCAGCAGAACCGCCTCTTCATCGTCTTCAAGCTCGTCTGCCAGCGCAGCCTCGGCGACAAAGACATCGGCCAGGTCGTCGTCCCCGTTAAGGAGCTTTTTGACTCCGTCAAGGCTGACGCCGCCTCCATGAAGTTCGTCGTTTACCAAGTCAGAAAGCCCTCTGGGAAGCCGAAAGGTGAGCTCAATTTTTCCTACAAATTTGGTGACAAAGTGGTGGCTCCACAGGCTGCGGCGACTGCGGCACCATCGAAGGGTAACGAGCCGGTAACAGCCTATCCGGCGGTTGGACCGTCAGTCCAGCCGATGGTGGGGTACGGTGGATACACCCCTCCAGTTGGCACCTCTGCTGGGCCTCCGCCATCTGGATACGAGTACCAACCGCCGCCGCCTGGATATGGTTACCCGCCACCGCCACCAGGATACGGCTACCCGCCACCACCACAGGGATACGGCTACCCGCCACCACCACCGGGGTATGGGTACCCGGGGCCGCCACCTCCGGGGTATGGGTACCCCGCGGTGCAGCAAACCCAACCGGAAGCAAAGAAGAAGAACAAGTTTGGGATGGGGCTGGGGGCTGGATTGTTAGGGGGAATGCTTGGTGGTTTGTTGATTGGAGATATGGTATCTGGTGGTGGTGGCTGCGGTGGCGGTGGCTGCGGCGGTGGCGGTGGATGTGGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

302

Amino Acids

31.32

Weight (kDa)

8.87

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 109 1.4e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 2 cut(s) 699, 729
AccB1I GGYRCC 4 cut(s) 460, 557, 699, 729
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 1 cut(s) 81
AccII CGCG 1 cut(s) 737
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 416
AcyI GRCGYC 2 cut(s) 54, 336
AfaI GTAC 5 cut(s) 212, 533, 593, 701, 731
AgsI TTSAA 1 cut(s) 247
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 5 cut(s) 15, 176, 288, 607, 634
AluBI AGCT 4 cut(s) 22, 250, 313, 400
AluI AGCT 4 cut(s) 22, 250, 313, 400
Alw21I GWGCWC 2 cut(s) 24, 402
Alw26I GTCTC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 703
AoxI GGCC 3 cut(s) 286, 569, 707
ApeKI GCWGC 6 cut(s) 127, 264, 446, 742, 861, 876
ApoI RAATTY 1 cut(s) 416
Asp718I GGTACC 2 cut(s) 699, 729
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 5 cut(s) 12, 165, 506, 569, 707
AsuC2I CCSGG 5 cut(s) 155, 690, 704, 705, 720
AsuHPI GGTGA 2 cut(s) 407, 434
AvaI CYCGRG 1 cut(s) 703
AvaII GGWCC 3 cut(s) 12, 165, 506
BanI GGYRCC 4 cut(s) 460, 557, 699, 729
BanII GRGCYC 2 cut(s) 24, 402
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 24, 402
BbvI GCAGC 6 cut(s) 139, 276, 433, 754, 848, 863
BccI CCATC 6 cut(s) 67, 177, 472, 517, 586, 778
BceAI ACGGC 3 cut(s) 71, 658, 685
BciT130I CCWGG 5 cut(s) 17, 178, 290, 609, 636
BciVI GTATCC 4 cut(s) 533, 578, 632, 659
BcnI CCSGG 5 cut(s) 155, 690, 704, 705, 720
BcoDI GTCTC 1 cut(s) 97
BfuI GTATCC 4 cut(s) 533, 578, 632, 659
BglI GCCNNNNNGGC 1 cut(s) 497
Bme18I GGWCC 3 cut(s) 12, 165, 506
BmeRI GACNNNNNGTC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 703
BmgT120I GGNCC 5 cut(s) 12, 165, 506, 569, 707
BmiI GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 701, 708, 731
BmsI GCATC 1 cut(s) 149
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 2 cut(s) 38, 534
BpuMI CCSGG 5 cut(s) 155, 690, 704, 705, 720
BsaHI GRCGYC 2 cut(s) 54, 336
BsaWI WCCGGW 1 cut(s) 754
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 828, 858
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 551
BseBI CCWGG 5 cut(s) 17, 178, 290, 609, 636
BseGI GGATG 4 cut(s) 164, 169, 789, 899
BseMII CTCAG 2 cut(s) 48, 233
BseNI ACTGG 1 cut(s) 551
BseXI GCAGC 6 cut(s) 139, 276, 433, 754, 848, 863
BseYI CCCAGC 2 cut(s) 566, 790
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 1 cut(s) 737
BshFI GGCC 3 cut(s) 288, 571, 709
BshNI GGYRCC 4 cut(s) 460, 557, 699, 729
BsiHKAI GWGCWC 2 cut(s) 24, 402
BsiHKCI CYCGRG 1 cut(s) 703
BsiSI CCGG 7 cut(s) 154, 482, 497, 689, 704, 719, 755
BslFI GGGAC 2 cut(s) 178, 284
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 178, 284
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 3 cut(s) 288, 571, 709
BsoBI CYCGRG 1 cut(s) 703
Bsp1286I GDGCHC 2 cut(s) 24, 402
BspANI GGCC 3 cut(s) 288, 571, 709
BspCNI CTCAG 2 cut(s) 47, 232
BspFNI CGCG 1 cut(s) 737
BspLI GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 701, 708, 731
BspT107I GGYRCC 4 cut(s) 460, 557, 699, 729
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 481
BssNAI GTATAC 1 cut(s) 82
BssNI GRCGYC 2 cut(s) 54, 336
BssT1I CCWWGG 2 cut(s) 39, 100
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 5 cut(s) 17, 178, 290, 609, 636
Bst4CI ACNGT 2 cut(s) 510, 536
Bst6I CTCTTC 1 cut(s) 239
BstACI GRCGYC 2 cut(s) 54, 336
BstC8I GCNNGC 2 cut(s) 125, 260
BstDEI CTNAG 2 cut(s) 34, 219
BstDSI CCRYGG 1 cut(s) 735
BstEII GGTNACC 1 cut(s) 617
BstF5I GGATG 4 cut(s) 164, 169, 789, 899
BstFNI CGCG 1 cut(s) 737
BstHHI GCGC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 97
BstNI CCWGG 5 cut(s) 17, 178, 290, 609, 636
BstPAI GACNNNNGTC 1 cut(s) 146
BstPI GGTNACC 1 cut(s) 617
BstUI CGCG 1 cut(s) 737
BstV1I GCAGC 6 cut(s) 139, 276, 433, 754, 848, 863
BstV2I GAAGAC 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 82
BsuI GTATCC 4 cut(s) 533, 578, 632, 659
BsuRI GGCC 3 cut(s) 288, 571, 709
BtgI CCRYGG 1 cut(s) 735
BtsCI GGATG 4 cut(s) 164, 169, 789, 899
Cac8I GCNNGC 2 cut(s) 125, 260
CfoI GCGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 5 cut(s) 12, 165, 506, 569, 707
Cfr42I CCGCGG 1 cut(s) 738
Cfr9I CCCGGG 1 cut(s) 703
CseI GACGC 1 cut(s) 344
Csp6I GTAC 5 cut(s) 211, 532, 592, 700, 730
CviAII CATG 2 cut(s) 64, 346
CviQI GTAC 5 cut(s) 211, 532, 592, 700, 730
DdeI CTNAG 2 cut(s) 34, 219
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 239
Eam1105I GACNNNNNGTC 1 cut(s) 512
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 22, 400
Eco130I CCWWGG 2 cut(s) 39, 100
Eco24I GRGCYC 2 cut(s) 24, 402
Eco47I GGWCC 3 cut(s) 12, 165, 506
Eco53kI GAGCTC 2 cut(s) 22, 400
Eco88I CYCGRG 1 cut(s) 703
Eco91I GGTNACC 1 cut(s) 617
EcoICRI GAGCTC 2 cut(s) 22, 400
EcoO109I RGGNCCY 2 cut(s) 12, 165
EcoO65I GGTNACC 1 cut(s) 617
EcoRII CCWGG 5 cut(s) 15, 176, 288, 607, 634
EcoT14I CCWWGG 2 cut(s) 39, 100
EcoT38I GRGCYC 2 cut(s) 24, 402
ErhI CCWWGG 2 cut(s) 39, 100
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 67, 349
FaiI YATR 7 cut(s) 65, 82, 347, 615, 696, 726, 842
FaqI GGGAC 2 cut(s) 178, 284
FatI CATG 2 cut(s) 63, 345
FauI CCCGC 5 cut(s) 155, 630, 657, 684, 742
FblI GTMKAC 1 cut(s) 81
FokI GGATG 3 cut(s) 156, 171, 796
FriOI GRGCYC 2 cut(s) 24, 402
GlaI GCGC 1 cut(s) 263
GsaI CCCAGC 2 cut(s) 570, 794
GsuI CTGGAG 2 cut(s) 38, 534
HaeIII GGCC 3 cut(s) 288, 571, 709
HapII CCGG 7 cut(s) 154, 482, 497, 689, 704, 719, 755
HgaI GACGC 1 cut(s) 344
HhaI GCGC 1 cut(s) 264
Hin1I GRCGYC 2 cut(s) 54, 336
Hin1II CATG 2 cut(s) 67, 349
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 2 cut(s) 113, 320
HpaII CCGG 7 cut(s) 154, 482, 497, 689, 704, 719, 755
HphI GGTGA 2 cut(s) 407, 434
Hpy166II GTNNAC 3 cut(s) 58, 82, 361
Hpy188I TCNGA 1 cut(s) 371
Hpy188III TCNNGA 2 cut(s) 203, 582
Hpy8I GTNNAC 3 cut(s) 58, 82, 361
Hpy99I CGWCG 6 cut(s) 92, 110, 113, 209, 299, 359
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 510, 536
HpyCH4IV ACGT 2 cut(s) 54, 78
HpyCH4V TGCA 1 cut(s) 742
HpyF3I CTNAG 2 cut(s) 34, 219
HpySE526I ACGT 2 cut(s) 54, 78
Hsp92I GRCGYC 2 cut(s) 54, 336
Hsp92II CATG 2 cut(s) 67, 349
HspAI GCGC 1 cut(s) 262
KflI GGGWCCC 1 cut(s) 165
KpnI GGTACC 2 cut(s) 703, 733
KspI CCGCGG 1 cut(s) 738
LmnI GCTCC 4 cut(s) 4, 19, 310, 442
Lsp1109I GCAGC 6 cut(s) 139, 276, 433, 754, 848, 863
LweI GCATC 1 cut(s) 149
MaeII ACGT 2 cut(s) 54, 78
MaeIII GTNAC 4 cut(s) 422, 473, 484, 617
MboII GAAGA 4 cut(s) 226, 235, 778, 781
MhlI GDGCHC 2 cut(s) 24, 402
MluCI AATT 2 cut(s) 403, 416
MlyI GAGTC 2 cut(s) 107, 314
MmeI TCCRAC 1 cut(s) 484
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 7 cut(s) 154, 482, 497, 689, 704, 719, 755
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 5 cut(s) 17, 178, 290, 609, 636
MvnI CGCG 1 cut(s) 737
NciI CCSGG 5 cut(s) 155, 690, 704, 705, 720
NlaIII CATG 2 cut(s) 67, 349
NlaIV GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 701, 708, 731
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 204, 291, 300
PctI GAATGC 1 cut(s) 819
PflFI GACNNNGTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 523
PleI GAGTC 2 cut(s) 107, 314
PpsI GAGTC 2 cut(s) 107, 314
PpuMI RGGWCCY 2 cut(s) 12, 165
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 24, 402
Psp5II RGGWCCY 2 cut(s) 12, 165
Psp6I CCWGG 5 cut(s) 15, 176, 288, 607, 634
PspEI GGTNACC 1 cut(s) 617
PspFI CCCAGC 2 cut(s) 566, 790
PspGI CCWGG 5 cut(s) 15, 176, 288, 607, 634
PspN4I GGNNCC 9 cut(s) 14, 166, 167, 438, 462, 559, 701, 708, 731
PspPI GGNCC 5 cut(s) 12, 165, 506, 569, 707
PspPPI RGGWCCY 2 cut(s) 12, 165
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 5 cut(s) 212, 533, 593, 701, 731
RsaNI GTAC 5 cut(s) 211, 532, 592, 700, 730
SacI GAGCTC 2 cut(s) 24, 402
SacII CCGCGG 1 cut(s) 738
SaqAI TTAA 1 cut(s) 306
Sau96I GGNCC 5 cut(s) 12, 165, 506, 569, 707
SchI GAGTC 2 cut(s) 107, 314
SduI GDGCHC 2 cut(s) 24, 402
SfaNI GCATC 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 738
SgrBI CCGCGG 1 cut(s) 738
SinI GGWCC 3 cut(s) 12, 165, 506
SmaI CCCGGG 1 cut(s) 705
Sse9I AATT 2 cut(s) 403, 416
SstI GAGCTC 2 cut(s) 24, 402
StyI CCWWGG 2 cut(s) 39, 100
TaaI ACNGT 2 cut(s) 510, 536
TaiI ACGT 2 cut(s) 57, 81
TaqI TCGA 3 cut(s) 24, 204, 467
TasI AATT 2 cut(s) 403, 416
TauI GCSGC 8 cut(s) 123, 341, 452, 461, 604, 607, 712, 882
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 6 cut(s) 127, 264, 446, 742, 861, 876
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 226, 362
TspGWI ACGGA 1 cut(s) 313
TspMI CCCGGG 1 cut(s) 703
Tth111I GACNNNGTC 1 cut(s) 323
Van91I CCANNNNNTGG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 12, 165, 506
XapI RAATTY 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 81
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.