FvH4_4g17890

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009797
Physical Location & Seq
Forward (+)
21853198 .. 21854448
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17890.t1

Sequence Viewer

Length: 717 bp
ATGGCTCTCTCTGAAACTTTAATGGAGCAACGGACTCTGGAACTACAGCTGGTCTCCGCCAAGAACCTGAAAAACGTGAACCTCCTCACCAAGATGGACGTCTACGCCGTGGTCTCCCTCAACGGCGACGCCTACAACGGCAATCAGAAGACCAAGACCAAACCCGTCCCAAACTGCGGCACCGACCCCACCTTCAACTTCACCCTGCACTTCAGCCTCGATGACTCCCTACTCATTAAACACAACGTACTCTCCCTCGAGGTCAAGCTCGTCAGCCAGCGCCTCCTTGGTGACAAGGTCATCGGCGCCGTCCACGTCCCCCTCAAGGAGCTCTTCGACTCCGCCGACCCCAGGGACATGAAGTTTATATCATACCAGGTCCGGCGACCTTCCGGCAAGCCCAAGGGCGAGCTCACCTTCTCTTATAAGTTCGGGGAAAAGGTCACGGTGCCGAAGCCGGCCAAGAAGAGTGAGCCGGTGATGGCGTACCCGGTTGGTGCAACAAAGTCGACGGGTTACCCGCCACAGGGTTACCCGACGGTGCAGCAGAAACCAGCAAAGAAGAAAAGTTCAGGGATGGGATTCGGTACTGGGTTAATTGGTGGGCTGTTGGTTGGAGATATGTTGTCCCACATTGGCGGTGGCGGATGCGGCGGCGGCGGCGGATGCGGCGGAGGATGCGGTGGAGGATGCGGCGGTGGGGGTTGTGGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

239

Amino Acids

24.87

Weight (kDa)

9.3

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 114 6.9e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AatII GACGTC 1 cut(s) 102
AbsI CCTCGAGG 1 cut(s) 257
AccB1I GGYRCC 3 cut(s) 179, 305, 448
AccI GTMKAC 2 cut(s) 102, 509
AcoI YGGCCR 1 cut(s) 459
AcuI CTGAAG 1 cut(s) 196
AcyI GRCGYC 3 cut(s) 99, 129, 306
AfaI GTAC 3 cut(s) 249, 488, 589
AfiI CCNNNNNNNGG 5 cut(s) 176, 325, 351, 526, 527
AgsI TTSAA 1 cut(s) 196
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 350, 375
AluBI AGCT 4 cut(s) 49, 268, 331, 412
AluI AGCT 4 cut(s) 49, 268, 331, 412
Alw21I GWGCWC 2 cut(s) 333, 414
Alw26I GTCTC 2 cut(s) 58, 118
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 544
AspLEI GCGC 2 cut(s) 282, 308
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 5 cut(s) 79, 193, 302, 406, 490
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 379
BanI GGYRCC 3 cut(s) 179, 305, 448
BanII GRGCYC 2 cut(s) 333, 414
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 333, 414
BbvI GCAGC 1 cut(s) 556
BccI CCATC 3 cut(s) 88, 475, 571
BceAI ACGGC 4 cut(s) 92, 139, 154, 293
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 2 cut(s) 352, 377
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 2 cut(s) 58, 118
BfmI CTRYAG 1 cut(s) 44
BfoI RGCGCY 2 cut(s) 283, 309
BisI GCNGC 8 cut(s) 178, 545, 652, 655, 658, 661, 670, 694
BlsI GCNGC 8 cut(s) 179, 546, 653, 656, 659, 662, 671, 695
Bme1390I CCNGG 3 cut(s) 352, 377, 491
Bme18I GGWCC 1 cut(s) 379
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 3 cut(s) 181, 307, 450
BmrFI CCNGG 3 cut(s) 352, 377, 491
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 4 cut(s) 638, 656, 668, 680
BmuI ACTGGG 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 491
BsaHI GRCGYC 3 cut(s) 99, 129, 306
BsaI GGTCTC 2 cut(s) 58, 118
BsaJI CCNNGG 5 cut(s) 108, 286, 350, 351, 402
BsaXI ACNNNNNCTCC 2 cut(s) 236, 266
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 325, 351, 526, 527
Bse118I RCCGGY 2 cut(s) 457, 475
Bse1I ACTGG 1 cut(s) 595
BseBI CCWGG 2 cut(s) 352, 377
BseDI CCNNGG 5 cut(s) 108, 286, 350, 351, 402
BseGI GGATG 5 cut(s) 582, 653, 671, 683, 695
BseLI CCNNNNNNNGG 5 cut(s) 176, 325, 351, 526, 527
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 1 cut(s) 556
BsgI GTGCAG 2 cut(s) 191, 563
BshFI GGCC 1 cut(s) 461
BshNI GGYRCC 3 cut(s) 179, 305, 448
BsiHKAI GWGCWC 2 cut(s) 333, 414
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 5 cut(s) 382, 393, 458, 476, 491
BslFI GGGAC 4 cut(s) 152, 302, 368, 613
BslI CCNNNNNNNGG 5 cut(s) 176, 325, 351, 526, 527
BsmAI GTCTC 2 cut(s) 58, 118
BsmFI GGGAC 4 cut(s) 152, 302, 368, 613
BsnI GGCC 1 cut(s) 461
Bso31I GGTCTC 2 cut(s) 58, 118
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 333, 414
BspANI GGCC 1 cut(s) 461
BspLI GGNNCC 3 cut(s) 181, 307, 450
BspQI GCTCTTC 1 cut(s) 338
BspT107I GGYRCC 3 cut(s) 179, 305, 448
BspTNI GGTCTC 2 cut(s) 58, 118
BsrFI RCCGGY 2 cut(s) 457, 475
BsrI ACTGG 1 cut(s) 595
BssAI RCCGGY 2 cut(s) 457, 475
BssECI CCNNGG 5 cut(s) 108, 286, 350, 351, 402
BssNI GRCGYC 3 cut(s) 99, 129, 306
BssT1I CCWWGG 2 cut(s) 286, 402
Bst2UI CCWGG 2 cut(s) 352, 377
Bst4CI ACNGT 2 cut(s) 448, 541
Bst6I CTCTTC 2 cut(s) 338, 461
BstACI GRCGYC 3 cut(s) 99, 129, 306
BstC8I GCNNGC 4 cut(s) 278, 398, 410, 459
BstDSI CCRYGG 1 cut(s) 108
BstEII GGTNACC 2 cut(s) 515, 530
BstF5I GGATG 5 cut(s) 582, 653, 671, 683, 695
BstH2I RGCGCY 2 cut(s) 283, 309
BstHHI GCGC 2 cut(s) 282, 308
BstMAI GTCTC 2 cut(s) 58, 118
BstMWI GCNNNNNNNGC 6 cut(s) 651, 657, 660, 666, 669, 678
BstNI CCWGG 2 cut(s) 352, 377
BstPI GGTNACC 2 cut(s) 515, 530
BstSCI CCNGG 3 cut(s) 350, 375, 489
BstSFI CTRYAG 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 556
BstV2I GAAGAC 1 cut(s) 155
BsuRI GGCC 1 cut(s) 461
BtgI CCRYGG 1 cut(s) 108
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 5 cut(s) 582, 653, 671, 683, 695
Cac8I GCNNGC 4 cut(s) 278, 398, 410, 459
CfoI GCGC 2 cut(s) 282, 308
Cfr10I RCCGGY 2 cut(s) 457, 475
Cfr13I GGNCC 1 cut(s) 379
CseI GACGC 1 cut(s) 137
CsiI ACCWGGT 1 cut(s) 375
Csp6I GTAC 3 cut(s) 248, 487, 588
CviAII CATG 1 cut(s) 358
CviQI GTAC 3 cut(s) 248, 487, 588
DinI GGCGCC 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 338, 461
EarI CTCTTC 2 cut(s) 338, 461
EciI GGCGGA 5 cut(s) 46, 331, 660, 678, 687
Ecl136II GAGCTC 2 cut(s) 331, 412
Eco130I CCWWGG 2 cut(s) 286, 402
Eco24I GRGCYC 2 cut(s) 333, 414
Eco31I GGTCTC 2 cut(s) 58, 118
Eco47I GGWCC 1 cut(s) 379
Eco53kI GAGCTC 2 cut(s) 331, 412
Eco57I CTGAAG 1 cut(s) 196
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 2 cut(s) 515, 530
EcoICRI GAGCTC 2 cut(s) 331, 412
EcoO65I GGTNACC 2 cut(s) 515, 530
EcoRII CCWGG 2 cut(s) 350, 375
EcoT14I CCWWGG 2 cut(s) 286, 402
EcoT38I GRGCYC 2 cut(s) 333, 414
EgeI GGCGCC 1 cut(s) 307
EheI GGCGCC 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 286, 402
FaeI CATG 1 cut(s) 361
FaiI YATR 5 cut(s) 359, 368, 373, 426, 623
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 4 cut(s) 152, 302, 368, 613
FatI CATG 1 cut(s) 357
FauI CCCGC 1 cut(s) 528
FblI GTMKAC 2 cut(s) 102, 509
Fnu4HI GCNGC 8 cut(s) 178, 545, 652, 655, 658, 661, 670, 694
FokI GGATG 5 cut(s) 589, 660, 678, 690, 702
FriOI GRGCYC 2 cut(s) 333, 414
Fsp4HI GCNGC 8 cut(s) 178, 545, 652, 655, 658, 661, 670, 694
GlaI GCGC 2 cut(s) 281, 307
GluI GCNGC 8 cut(s) 178, 545, 652, 655, 658, 661, 670, 694
HaeII RGCGCY 2 cut(s) 283, 309
HaeIII GGCC 1 cut(s) 461
HapII CCGG 5 cut(s) 382, 393, 458, 476, 491
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 282, 308
Hin1I GRCGYC 3 cut(s) 99, 129, 306
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 280, 306
HinP1I GCGC 2 cut(s) 280, 306
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 4 cut(s) 34, 224, 338, 582
HpaII CCGG 5 cut(s) 382, 393, 458, 476, 491
HphI GGTGA 5 cut(s) 79, 193, 302, 406, 490
Hpy166II GTNNAC 4 cut(s) 79, 103, 313, 510
Hpy188I TCNGA 2 cut(s) 13, 147
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 4 cut(s) 79, 103, 313, 510
Hpy99I CGWCG 3 cut(s) 131, 514, 541
HpyAV CCTTC 3 cut(s) 202, 399, 427
HpyCH4III ACNGT 2 cut(s) 448, 541
HpyCH4IV ACGT 4 cut(s) 75, 99, 246, 315
HpyCH4V TGCA 3 cut(s) 208, 500, 544
HpyF10VI GCNNNNNNNGC 6 cut(s) 651, 657, 660, 666, 669, 678
HpySE526I ACGT 4 cut(s) 75, 99, 246, 315
Hsp92I GRCGYC 3 cut(s) 99, 129, 306
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 280, 306
KasI GGCGCC 1 cut(s) 305
KroI GCCGGC 1 cut(s) 457
KroNI GCCGGC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 2 cut(s) 25, 328
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 4 cut(s) 638, 656, 668, 680
MabI ACCWGGT 1 cut(s) 375
MaeII ACGT 4 cut(s) 75, 99, 246, 315
MaeIII GTNAC 4 cut(s) 290, 442, 515, 530
MboII GAAGA 4 cut(s) 160, 325, 478, 574
MhlI GDGCHC 2 cut(s) 333, 414
MluCI AATT 1 cut(s) 597
Mly113I GGCGCC 1 cut(s) 306
MlyI GAGTC 3 cut(s) 28, 218, 332
MmeI TCCRAC 1 cut(s) 595
MroNI GCCGGC 1 cut(s) 457
MseI TTAA 3 cut(s) 20, 237, 596
MslI CAYNNNNRTG 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 5 cut(s) 382, 393, 458, 476, 491
MspR9I CCNGG 3 cut(s) 352, 377, 491
MvaI CCWGG 2 cut(s) 352, 377
MwoI GCNNNNNNNGC 6 cut(s) 651, 657, 660, 666, 669, 678
NaeI GCCGGC 1 cut(s) 459
NarI GGCGCC 1 cut(s) 306
NciI CCSGG 1 cut(s) 491
NgoMIV GCCGGC 1 cut(s) 457
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 3 cut(s) 181, 307, 450
NmuCI GTSAC 2 cut(s) 290, 442
PaeR7I CTCGAG 1 cut(s) 257
PasI CCCWGGG 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 342
PdiI GCCGGC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 582
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 8 cut(s) 179, 546, 653, 656, 659, 662, 671, 695
PleI GAGTC 3 cut(s) 28, 218, 332
PluTI GGCGCC 1 cut(s) 309
PpsI GAGTC 3 cut(s) 28, 218, 332
PsiI TTATAA 1 cut(s) 426
Psp124BI GAGCTC 2 cut(s) 333, 414
Psp6I CCWGG 2 cut(s) 350, 375
PspEI GGTNACC 2 cut(s) 515, 530
PspGI CCWGG 2 cut(s) 350, 375
PspN4I GGNNCC 3 cut(s) 181, 307, 450
PspPI GGNCC 1 cut(s) 379
PspXI VCTCGAGB 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 49
RsaI GTAC 3 cut(s) 249, 488, 589
RsaNI GTAC 3 cut(s) 248, 487, 588
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 2 cut(s) 333, 414
SalI GTCGAC 1 cut(s) 508
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 3 cut(s) 20, 237, 596
SatI GCNGC 8 cut(s) 178, 545, 652, 655, 658, 661, 670, 694
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 3 cut(s) 28, 218, 332
ScrFI CCNGG 3 cut(s) 352, 377, 491
SduI GDGCHC 2 cut(s) 333, 414
SexAI ACCWGGT 1 cut(s) 375
SfaNI GCATC 4 cut(s) 638, 656, 668, 680
SfcI CTRYAG 1 cut(s) 44
SfoI GGCGCC 1 cut(s) 307
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 379
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 257, 323
SmoI CTYRAG 2 cut(s) 257, 323
Sse9I AATT 1 cut(s) 597
SspDI GGCGCC 1 cut(s) 305
SstI GAGCTC 2 cut(s) 333, 414
StyD4I CCNGG 3 cut(s) 350, 375, 489
StyI CCWWGG 2 cut(s) 286, 402
TaaI ACNGT 2 cut(s) 448, 541
TaiI ACGT 4 cut(s) 78, 102, 249, 318
TaqI TCGA 4 cut(s) 219, 258, 336, 509
TasI AATT 1 cut(s) 597
TauI GCSGC 7 cut(s) 180, 654, 657, 660, 663, 672, 696
TfiI GAWTC 1 cut(s) 582
Tru1I TTAA 3 cut(s) 20, 237, 596
Tru9I TTAA 3 cut(s) 20, 237, 596
TseFI GTSAC 2 cut(s) 290, 442
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 2 cut(s) 290, 442
TspDTI ATGAA 1 cut(s) 374
TspGWI ACGGA 1 cut(s) 46
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 379
XcmI CCANNNNNNNNNTGG 2 cut(s) 284, 638
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 2 cut(s) 102, 509
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.