Prupe.1G138000_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009797
Physical Location & Seq
Reverse (-)
10799790 .. 10800668
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G138000.1

Sequence Viewer

Length: 879 bp
ATGGAGCAGAGAACCCTAGAACTCGAACTCATCTCAGCCAAGGACCTCAAAGACGTCAACTTCATCTCCAAGATGGACGTATACGCCGTCGTTTCACTCCAGGGCGACGACTCGCACGGCAAGCAGAAAACTAAAACCAAGGTGGCGAAAGACTGCGGGACCCACCCCACCTGGAACTTCCCCATGAAATTCACCCTCGACGAGCTTCTTCTCAACCGCCTTAGCCTCGAGTTCAAGCTCGTCTGCGATCGCAGCCTCGGCGACAAAGATATAGGCCAAGTCGTCGTCCCCGTTAAAGAGCTCTTCGACTCCGTGGCGGCGGCAGCAGCTGACCCCAACTCCATGAAGTTCGTTGCTTACCAGGTCAGAAAACCATCCGGAAAACCCAAAGGCGAACTTCATTTCTCCTACAAATTTGGCGACAAAGTCACCGCCCCAGTGACGGCAGCGCCCCCGAAGGGTTATGAGTCCGTAACAGCAGCGCCGCCGAAGGGTTATGAGCCCGTGACGGCGTATCCGATGGTCGGGCCATCGGCGCCGACAATGACGGGATACAGCGCTTACCCGCCTCCAGCTGGCAGCTCAACGGGACCGCCGCCACCACCTGAATACGGGTACCCACCACCACCTCCGGGATACGGATACCCCCCGCCACCGGGGTATGGGTACCCGCCTCCGCCGCCGGGGTATGGATACCCTCCGGTGCAGCAACCCCCGGTCCAAGAGAAGAAGAAGAATAAGTTTGGGATGGGGCTGGGGGCTGGATTGTTAGGAGGGATGCTTGGTGGGATGTTGATCGGAGATATAGTATCTGATGGTGGTGGTGGTTGTGGCGGCGGCGGTGGATGTGGTGGCGGTGGCTGCGGTGGTGGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

293

Amino Acids

30.41

Weight (kDa)

8.12

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 2 cut(s) 615, 666
AccB1I GGYRCC 3 cut(s) 535, 615, 666
AccI GTMKAC 1 cut(s) 81
AccIII TCCGGA 1 cut(s) 377
AcsI RAATTY 2 cut(s) 188, 413
AcyI GRCGYC 2 cut(s) 54, 536
AfaI GTAC 2 cut(s) 617, 668
AfeI AGCGCT 1 cut(s) 559
AgsI TTSAA 1 cut(s) 235
AjnI CCWGG 3 cut(s) 99, 170, 360
AluBI AGCT 6 cut(s) 205, 238, 301, 329, 575, 582
AluI AGCT 6 cut(s) 205, 238, 301, 329, 575, 582
Alw21I GWGCWC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 377
Aor51HI AGCGCT 1 cut(s) 559
AoxI GGCC 2 cut(s) 274, 527
ApeKI GCWGC 8 cut(s) 252, 323, 326, 446, 479, 579, 706, 861
ApoI RAATTY 2 cut(s) 188, 413
AsiSI GCGATCGC 1 cut(s) 250
Asp718I GGTACC 2 cut(s) 615, 666
AspLEI GCGC 4 cut(s) 451, 484, 538, 560
AspS9I GGNCC 5 cut(s) 43, 159, 527, 590, 718
AsuC2I CCSGG 4 cut(s) 633, 657, 684, 716
AsuHPI GGTGA 2 cut(s) 184, 421
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 4 cut(s) 43, 159, 590, 718
BanI GGYRCC 3 cut(s) 535, 615, 666
BanII GRGCYC 2 cut(s) 303, 504
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 8 cut(s) 264, 335, 338, 458, 491, 591, 718, 848
BccI CCATC 6 cut(s) 67, 382, 514, 538, 742, 809
BceAI ACGGC 4 cut(s) 71, 133, 459, 525
BciT130I CCWGG 3 cut(s) 101, 172, 362
BciVI GTATCC 5 cut(s) 525, 545, 629, 635, 686
BcnI CCSGG 4 cut(s) 633, 657, 684, 716
BfaI CTAG 1 cut(s) 17
BfoI RGCGCY 4 cut(s) 452, 485, 539, 561
BfuI GTATCC 5 cut(s) 525, 545, 629, 635, 686
Bme1390I CCNGG 7 cut(s) 101, 172, 362, 633, 657, 684, 716
Bme18I GGWCC 4 cut(s) 43, 159, 590, 718
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 5 cut(s) 43, 159, 527, 590, 718
BmiI GGNNCC 6 cut(s) 160, 161, 537, 591, 617, 668
BmrFI CCNGG 7 cut(s) 101, 172, 362, 633, 657, 684, 716
BmrI ACTGGG 1 cut(s) 431
BmsI GCATC 1 cut(s) 768
BmuI ACTGGG 1 cut(s) 431
BpmI CTGGAG 2 cut(s) 83, 555
Bpu10I CCTNAGC 1 cut(s) 221
BpuMI CCSGG 4 cut(s) 633, 657, 684, 716
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 2 cut(s) 54, 536
BsaJI CCNNGG 8 cut(s) 39, 100, 138, 256, 312, 656, 683, 714
BsaWI WCCGGW 2 cut(s) 377, 700
BsaXI ACNNNNNCTCC 6 cut(s) 50, 80, 323, 353, 792, 822
Bse1I ACTGG 1 cut(s) 437
Bse8I GATNNNNATC 1 cut(s) 794
BseAI TCCGGA 1 cut(s) 377
BseBI CCWGG 3 cut(s) 101, 172, 362
BseDI CCNNGG 8 cut(s) 39, 100, 138, 256, 312, 656, 683, 714
BseGI GGATG 5 cut(s) 374, 753, 783, 795, 851
BseJI GATNNNNATC 1 cut(s) 794
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 437
BseXI GCAGC 8 cut(s) 264, 335, 338, 458, 491, 591, 718, 848
BseYI CCCAGC 1 cut(s) 754
BsgI GTGCAG 1 cut(s) 725
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 2 cut(s) 276, 529
BshNI GGYRCC 3 cut(s) 535, 615, 666
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 6 cut(s) 378, 632, 656, 683, 701, 716
BslFI GGGAC 3 cut(s) 172, 272, 603
BsmFI GGGAC 3 cut(s) 172, 272, 603
BsnI GGCC 2 cut(s) 276, 529
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 303, 504
Bsp13I TCCGGA 1 cut(s) 377
Bsp143I GATC 2 cut(s) 247, 795
BspANI GGCC 2 cut(s) 276, 529
BspCNI CTCAG 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 377
BspLI GGNNCC 6 cut(s) 160, 161, 537, 591, 617, 668
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 3 cut(s) 535, 615, 666
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 8 cut(s) 39, 100, 138, 256, 312, 656, 683, 714
BssMI GATC 2 cut(s) 247, 795
BssNAI GTATAC 1 cut(s) 82
BssNI GRCGYC 2 cut(s) 54, 536
BssT1I CCWWGG 2 cut(s) 39, 138
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 3 cut(s) 101, 172, 362
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 2 cut(s) 54, 536
BstC8I GCNNGC 2 cut(s) 122, 577
BstDEI CTNAG 2 cut(s) 34, 221
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 5 cut(s) 374, 753, 783, 795, 851
BstH2I RGCGCY 4 cut(s) 452, 485, 539, 561
BstHHI GCGC 4 cut(s) 451, 484, 538, 560
BstKTI GATC 2 cut(s) 250, 798
BstMBI GATC 2 cut(s) 247, 795
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 8 cut(s) 121, 252, 258, 323, 326, 535, 679, 861
BstNI CCWGG 3 cut(s) 101, 172, 362
BstSCI CCNGG 7 cut(s) 99, 170, 360, 631, 655, 682, 714
BstV1I GCAGC 8 cut(s) 264, 335, 338, 458, 491, 591, 718, 848
BstZ17I GTATAC 1 cut(s) 82
BsuI GTATCC 5 cut(s) 525, 545, 629, 635, 686
BsuRI GGCC 2 cut(s) 276, 529
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 5 cut(s) 374, 753, 783, 795, 851
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 122, 577
CaiI CAGNNNCTG 1 cut(s) 329
CfoI GCGC 4 cut(s) 451, 484, 538, 560
Cfr13I GGNCC 5 cut(s) 43, 159, 527, 590, 718
CsiI ACCWGGT 1 cut(s) 360
Csp6I GTAC 2 cut(s) 616, 667
CviAII CATG 2 cut(s) 184, 343
CviQI GTAC 2 cut(s) 616, 667
DdeI CTNAG 2 cut(s) 34, 221
DinI GGCGCC 1 cut(s) 537
DpnI GATC 2 cut(s) 249, 797
DpnII GATC 2 cut(s) 247, 795
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 666
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 39, 138
Eco24I GRGCYC 2 cut(s) 303, 504
Eco47I GGWCC 4 cut(s) 43, 159, 590, 718
Eco47III AGCGCT 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 2 cut(s) 43, 159
EcoRII CCWGG 3 cut(s) 99, 170, 360
EcoT14I CCWWGG 2 cut(s) 39, 138
EcoT38I GRGCYC 2 cut(s) 303, 504
EgeI GGCGCC 1 cut(s) 537
EheI GGCGCC 1 cut(s) 537
ErhI CCWWGG 2 cut(s) 39, 138
FaeI CATG 2 cut(s) 187, 346
FaiI YATR 9 cut(s) 82, 185, 272, 344, 465, 498, 663, 690, 806
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 3 cut(s) 172, 272, 603
FatI CATG 2 cut(s) 183, 342
FauI CCCGC 4 cut(s) 149, 573, 657, 678
FblI GTMKAC 1 cut(s) 81
FokI GGATG 5 cut(s) 361, 760, 790, 802, 858
FriOI GRGCYC 2 cut(s) 303, 504
FspBI CTAG 1 cut(s) 17
GlaI GCGC 4 cut(s) 450, 483, 537, 559
GsaI CCCAGC 1 cut(s) 758
GsuI CTGGAG 2 cut(s) 83, 555
HaeII RGCGCY 4 cut(s) 452, 485, 539, 561
HaeIII GGCC 2 cut(s) 276, 529
HapII CCGG 6 cut(s) 378, 632, 656, 683, 701, 716
HhaI GCGC 4 cut(s) 451, 484, 538, 560
Hin1I GRCGYC 2 cut(s) 54, 536
Hin1II CATG 2 cut(s) 187, 346
Hin6I GCGC 4 cut(s) 449, 482, 536, 558
HinP1I GCGC 4 cut(s) 449, 482, 536, 558
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 3 cut(s) 110, 308, 467
HpaII CCGG 6 cut(s) 378, 632, 656, 683, 701, 716
HphI GGTGA 2 cut(s) 184, 421
Hpy166II GTNNAC 2 cut(s) 58, 82
Hpy188I TCNGA 4 cut(s) 368, 519, 800, 814
Hpy188III TCNNGA 1 cut(s) 378
Hpy8I GTNNAC 2 cut(s) 58, 82
Hpy99I CGWCG 4 cut(s) 92, 110, 203, 287
HpyAV CCTTC 2 cut(s) 451, 484
HpyCH4IV ACGT 2 cut(s) 54, 78
HpyCH4V TGCA 1 cut(s) 706
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 252, 258, 323, 326, 535, 679, 861
HpyF3I CTNAG 2 cut(s) 34, 221
HpySE526I ACGT 2 cut(s) 54, 78
Hsp92I GRCGYC 2 cut(s) 54, 536
Hsp92II CATG 2 cut(s) 187, 346
HspAI GCGC 4 cut(s) 449, 482, 536, 558
KasI GGCGCC 1 cut(s) 535
KflI GGGWCCC 1 cut(s) 159
Kpn2I TCCGGA 1 cut(s) 377
KpnI GGTACC 2 cut(s) 619, 670
Kzo9I GATC 2 cut(s) 247, 795
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 8 cut(s) 264, 335, 338, 458, 491, 591, 718, 848
LweI GCATC 1 cut(s) 768
MabI ACCWGGT 1 cut(s) 360
MaeI CTAG 1 cut(s) 17
MaeII ACGT 2 cut(s) 54, 78
MaeIII GTNAC 4 cut(s) 427, 439, 472, 505
MalI GATC 2 cut(s) 249, 797
MboI GATC 2 cut(s) 247, 795
MboII GAAGA 5 cut(s) 200, 295, 739, 742, 745
MhlI GDGCHC 2 cut(s) 303, 504
MluCI AATT 2 cut(s) 188, 413
Mly113I GGCGCC 1 cut(s) 536
MlyI GAGTC 3 cut(s) 104, 302, 476
MnlI CCTC 9 cut(s) 56, 206, 236, 266, 579, 639, 684, 708, 767
MroI TCCGGA 1 cut(s) 377
MseI TTAA 2 cut(s) 294, 877
MspA1I CMGCKG 2 cut(s) 329, 575
MspI CCGG 6 cut(s) 378, 632, 656, 683, 701, 716
MspR9I CCNGG 7 cut(s) 101, 172, 362, 633, 657, 684, 716
MvaI CCWGG 3 cut(s) 101, 172, 362
MwoI GCNNNNNNNGC 8 cut(s) 121, 252, 258, 323, 326, 535, 679, 861
NarI GGCGCC 1 cut(s) 536
NciI CCSGG 4 cut(s) 633, 657, 684, 716
NdeII GATC 2 cut(s) 247, 795
NlaIII CATG 2 cut(s) 187, 346
NlaIV GGNNCC 6 cut(s) 160, 161, 537, 591, 617, 668
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 3 cut(s) 427, 439, 505
PaeR7I CTCGAG 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 288, 515
PflFI GACNNNGTC 1 cut(s) 425
PfoI TCCNGGA 1 cut(s) 631
Ple19I CGATCG 1 cut(s) 250
PleI GAGTC 3 cut(s) 104, 302, 475
PluTI GGCGCC 1 cut(s) 539
PpsI GAGTC 3 cut(s) 104, 302, 475
PpuMI RGGWCCY 2 cut(s) 43, 159
Psp124BI GAGCTC 1 cut(s) 303
Psp5II RGGWCCY 2 cut(s) 43, 159
Psp6I CCWGG 3 cut(s) 99, 170, 360
PspFI CCCAGC 1 cut(s) 754
PspGI CCWGG 3 cut(s) 99, 170, 360
PspN4I GGNNCC 6 cut(s) 160, 161, 537, 591, 617, 668
PspPI GGNCC 5 cut(s) 43, 159, 527, 590, 718
PspPPI RGGWCCY 2 cut(s) 43, 159
PspXI VCTCGAGB 1 cut(s) 227
PstNI CAGNNNCTG 1 cut(s) 329
PsyI GACNNNGTC 1 cut(s) 425
PvuI CGATCG 1 cut(s) 250
PvuII CAGCTG 2 cut(s) 329, 575
RgaI GCGATCGC 1 cut(s) 250
RsaI GTAC 2 cut(s) 617, 668
RsaNI GTAC 2 cut(s) 616, 667
SacI GAGCTC 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 294, 877
Sau3AI GATC 2 cut(s) 247, 795
Sau96I GGNCC 5 cut(s) 43, 159, 527, 590, 718
SchI GAGTC 3 cut(s) 104, 302, 476
ScrFI CCNGG 7 cut(s) 101, 172, 362, 633, 657, 684, 716
SduI GDGCHC 2 cut(s) 303, 504
SexAI ACCWGGT 1 cut(s) 360
SfaAI GCGATCGC 1 cut(s) 250
SfaNI GCATC 1 cut(s) 768
SfoI GGCGCC 1 cut(s) 537
Sfr274I CTCGAG 1 cut(s) 227
SgfI GCGATCGC 1 cut(s) 250
SinI GGWCC 4 cut(s) 43, 159, 590, 718
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 188, 413
SspDI GGCGCC 1 cut(s) 535
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 7 cut(s) 99, 170, 360, 631, 655, 682, 714
StyI CCWWGG 2 cut(s) 39, 138
TaiI ACGT 2 cut(s) 57, 81
TaqI TCGA 4 cut(s) 24, 198, 228, 306
TasI AATT 2 cut(s) 188, 413
TauI GCSGC 7 cut(s) 320, 323, 487, 598, 682, 837, 840
Tru1I TTAA 2 cut(s) 294, 877
Tru9I TTAA 2 cut(s) 294, 877
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 3 cut(s) 427, 439, 505
TseI GCWGC 8 cut(s) 252, 323, 326, 446, 479, 579, 706, 861
Tsp45I GTSAC 3 cut(s) 427, 439, 505
TspDTI ATGAA 4 cut(s) 52, 200, 359, 389
TspGWI ACGGA 3 cut(s) 301, 460, 654
TspRI CASTG 1 cut(s) 444
Tth111I GACNNNGTC 1 cut(s) 425
VpaK11BI GGWCC 4 cut(s) 43, 159, 590, 718
XapI RAATTY 2 cut(s) 188, 413
XhoI CTCGAG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.