Rh4CG250600

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009797
Physical Location & Seq
Forward (+)
50063436 .. 50064326
891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG250600.1

Sequence Viewer

Length: 891 bp
ATGGAGCAAAGGACTCTGGAGCTGGAGCTGGTCTCCGCCAAGGACCTGAAGGACGTGAACCTCATCTCCAAGATGGACGTCTACGCCGTCGTCTCCCTCGACGGCGACGCCTACTACGGCAAGCAGAAGACCAAGACCAAAGTCGTCCGCGCCTGCGGCACCAACCCCACCTTCAACTTCCCGATGAGGTTCACCGTCGACGACACTCTCACCCAACAGAACCGCCTCTCCCTCCAAGTCAAGCTCGTCTGCCAGCGCAGCCTCGGCGACAAGGACATCGGCGTCGTTTACGTCCCCGTCAAGGAGCTCTTCGACTCCGCCGACCCCAAGAACATGAAGTTCATATCGTACCAGGTCCGACGACCCTCCGGGAAGCCCAAGGGCGAGCTCAGCTTTTCTTATAAATTTGGGGAGAAGGTCGCCGCGCCGGTACGGGAGGTGGCCAAGAAGGGGGAGCCGGTGACGGCTTATCCGGTTGGAGGTCCGTCCGCGCCGGCGTACGGGGCGTACCCTCTTCAGGCGACGAGTTCGTCCGGGTCGGGTTACCCGCCGCCTCAACCCGGGTATGGCTACCCGCCGCCGCCGCCACCTGGGGCTGGATATCCGGAAGCGCCTCCACCGGGTTACGGGTACCCACCGCAGCAACCGGGCTACGCTTACCCGCCTCAGCAGCCCGGGTACGGATACCCACCGCCGCCGCCGGGTTACGGGTACCCGCCGGTGCAGCAAGTGCAGCAACCGGAAAAGAAGAAGAGCAAGTTTGGAATGGGATTGGGCGCTGGGTTGCTAGGAGGTGCCCTTGGTGGGTTGTTGATTGGAGATATGATTTCCGATGGAGGCGGTGGATGCGGTGGCGGCGGCTGCGGCGGCGGCGGTTGCGGTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

296

Amino Acids

31.08

Weight (kDa)

8.96

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 107 2.4e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AasI GACNNNNNNGTC 2 cut(s) 281, 529
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 2 cut(s) 630, 711
AccB1I GGYRCC 4 cut(s) 158, 630, 711, 794
AccI GTMKAC 2 cut(s) 81, 198
AccII CGCG 3 cut(s) 150, 425, 491
AccIII TCCGGA 1 cut(s) 604
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 68, 500
AcyI GRCGYC 3 cut(s) 78, 108, 282
AfaI GTAC 7 cut(s) 350, 432, 500, 509, 632, 680, 713
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 351, 589
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AloI GAACNNNNNNTCC 4 cut(s) 50, 82, 212, 244
AluBI AGCT 6 cut(s) 22, 28, 244, 307, 388, 393
AluI AGCT 6 cut(s) 22, 28, 244, 307, 388, 393
Alw21I GWGCWC 2 cut(s) 309, 390
Alw26I GTCTC 2 cut(s) 37, 97
Ama87I CYCGRG 2 cut(s) 560, 674
Aor13HI TCCGGA 1 cut(s) 604
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 6 cut(s) 258, 640, 670, 724, 733, 861
ApoI RAATTY 1 cut(s) 404
Asp718I GGTACC 2 cut(s) 630, 711
AspLEI GCGC 6 cut(s) 152, 258, 427, 493, 613, 779
AspS9I GGNCC 3 cut(s) 43, 355, 482
AsuC2I CCSGG 9 cut(s) 370, 535, 561, 562, 621, 648, 675, 676, 702
AsuHPI GGTGA 3 cut(s) 184, 202, 472
AvaI CYCGRG 2 cut(s) 560, 674
AvaII GGWCC 3 cut(s) 43, 355, 482
BaeGI GKGCMC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 4 cut(s) 158, 630, 711, 794
BanII GRGCYC 2 cut(s) 309, 390
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 309, 390
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 6 cut(s) 270, 652, 682, 736, 745, 848
BccI CCATC 2 cut(s) 67, 827
BceAI ACGGC 4 cut(s) 71, 118, 133, 480
BciT130I CCWGG 2 cut(s) 353, 591
BciVI GTATCC 1 cut(s) 677
BcnI CCSGG 9 cut(s) 370, 535, 561, 562, 621, 648, 675, 676, 702
BcoDI GTCTC 2 cut(s) 37, 97
BfaI CTAG 1 cut(s) 788
BfoI RGCGCY 2 cut(s) 614, 780
BfuI GTATCC 1 cut(s) 677
BlpI GCTNAGC 1 cut(s) 389
Bme18I GGWCC 3 cut(s) 43, 355, 482
BmeT110I CYCGRG 2 cut(s) 560, 674
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 43, 355, 482
BmiI GGNNCC 5 cut(s) 160, 456, 632, 713, 796
BmsI GCATC 1 cut(s) 836
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 2 cut(s) 38, 44
Bpu10I CCTNAGC 1 cut(s) 666
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 9 cut(s) 370, 535, 561, 562, 621, 648, 675, 676, 702
BsaHI GRCGYC 3 cut(s) 78, 108, 282
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 7 cut(s) 39, 262, 378, 560, 590, 674, 799
BsaWI WCCGGW 3 cut(s) 472, 604, 739
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 212, 242
Bse118I RCCGGY 4 cut(s) 427, 457, 493, 718
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 2 cut(s) 353, 591
BseDI CCNNGG 7 cut(s) 39, 262, 378, 560, 590, 674, 799
BseGI GGATG 1 cut(s) 851
BseMII CTCAG 2 cut(s) 403, 680
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 6 cut(s) 270, 652, 682, 736, 745, 848
BseYI CCCAGC 1 cut(s) 779
BsgI GTGCAG 2 cut(s) 743, 752
Bsh1236I CGCG 3 cut(s) 150, 425, 491
BshFI GGCC 1 cut(s) 443
BshNI GGYRCC 4 cut(s) 158, 630, 711, 794
BsiHKAI GWGCWC 2 cut(s) 309, 390
BsiHKCI CYCGRG 2 cut(s) 560, 674
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 37, 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 443
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 560, 674
Bsp1286I GDGCHC 3 cut(s) 309, 390, 799
Bsp13I TCCGGA 1 cut(s) 604
Bsp1720I GCTNAGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 443
BspCNI CTCAG 2 cut(s) 402, 679
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 3 cut(s) 150, 425, 491
BspLI GGNNCC 5 cut(s) 160, 456, 632, 713, 796
BspQI GCTCTTC 2 cut(s) 314, 746
BspT107I GGYRCC 4 cut(s) 158, 630, 711, 794
BspTNI GGTCTC 1 cut(s) 37
BsrFI RCCGGY 4 cut(s) 427, 457, 493, 718
BssAI RCCGGY 4 cut(s) 427, 457, 493, 718
BssECI CCNNGG 7 cut(s) 39, 262, 378, 560, 590, 674, 799
BssNI GRCGYC 3 cut(s) 78, 108, 282
BssT1I CCWWGG 3 cut(s) 39, 378, 799
Bst2UI CCWGG 2 cut(s) 353, 591
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 3 cut(s) 314, 519, 746
BstACI GRCGYC 3 cut(s) 78, 108, 282
BstAPI GCANNNNNTGC 1 cut(s) 730
BstC8I GCNNGC 5 cut(s) 122, 154, 254, 386, 495
BstDEI CTNAG 2 cut(s) 389, 666
BstEII GGTNACC 1 cut(s) 542
BstF5I GGATG 1 cut(s) 851
BstFNI CGCG 3 cut(s) 150, 425, 491
BstH2I RGCGCY 2 cut(s) 614, 780
BstHHI GCGC 6 cut(s) 152, 258, 427, 493, 613, 779
BstMAI GTCTC 2 cut(s) 37, 97
BstNI CCWGG 2 cut(s) 353, 591
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 542
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 3 cut(s) 150, 425, 491
BstV1I GCAGC 6 cut(s) 270, 652, 682, 736, 745, 848
BstV2I GAAGAC 1 cut(s) 134
BsuI GTATCC 1 cut(s) 677
BsuRI GGCC 1 cut(s) 443
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 1 cut(s) 851
Cac8I GCNNGC 5 cut(s) 122, 154, 254, 386, 495
CfoI GCGC 6 cut(s) 152, 258, 427, 493, 613, 779
Cfr10I RCCGGY 4 cut(s) 427, 457, 493, 718
Cfr13I GGNCC 3 cut(s) 43, 355, 482
Cfr9I CCCGGG 2 cut(s) 560, 674
CseI GACGC 2 cut(s) 116, 271
CsiI ACCWGGT 1 cut(s) 351
Csp6I GTAC 7 cut(s) 349, 431, 499, 508, 631, 679, 712
CviAII CATG 1 cut(s) 334
CviQI GTAC 7 cut(s) 349, 431, 499, 508, 631, 679, 712
DdeI CTNAG 2 cut(s) 389, 666
DrdI GACNNNNNNGTC 2 cut(s) 281, 529
DseDI GACNNNNNNGTC 2 cut(s) 281, 529
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 3 cut(s) 314, 519, 746
EarI CTCTTC 3 cut(s) 314, 519, 746
EciI GGCGGA 2 cut(s) 25, 307
Ecl136II GAGCTC 2 cut(s) 307, 388
Eco130I CCWWGG 3 cut(s) 39, 378, 799
Eco24I GRGCYC 2 cut(s) 309, 390
Eco31I GGTCTC 1 cut(s) 37
Eco32I GATATC 1 cut(s) 602
Eco47I GGWCC 3 cut(s) 43, 355, 482
Eco53kI GAGCTC 2 cut(s) 307, 388
Eco57I CTGAAG 2 cut(s) 68, 500
Eco88I CYCGRG 2 cut(s) 560, 674
Eco91I GGTNACC 1 cut(s) 542
EcoICRI GAGCTC 2 cut(s) 307, 388
EcoO109I RGGNCCY 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 351, 589
EcoRV GATATC 1 cut(s) 602
EcoT14I CCWWGG 3 cut(s) 39, 378, 799
EcoT38I GRGCYC 2 cut(s) 309, 390
ErhI CCWWGG 3 cut(s) 39, 378, 799
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 1 cut(s) 337
FaiI YATR 5 cut(s) 335, 344, 402, 567, 824
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 333
FauI CCCGC 4 cut(s) 555, 582, 669, 723
FblI GTMKAC 2 cut(s) 81, 198
FokI GGATG 1 cut(s) 858
FriOI GRGCYC 2 cut(s) 309, 390
FspBI CTAG 1 cut(s) 788
GlaI GCGC 6 cut(s) 151, 257, 426, 492, 612, 778
GsaI CCCAGC 1 cut(s) 783
GsuI CTGGAG 2 cut(s) 38, 44
HaeII RGCGCY 2 cut(s) 614, 780
HaeIII GGCC 1 cut(s) 443
HgaI GACGC 2 cut(s) 116, 271
HhaI GCGC 6 cut(s) 152, 258, 427, 493, 613, 779
Hin1I GRCGYC 3 cut(s) 78, 108, 282
Hin1II CATG 1 cut(s) 337
Hin6I GCGC 6 cut(s) 150, 256, 425, 491, 611, 777
HinP1I GCGC 6 cut(s) 150, 256, 425, 491, 611, 777
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 2 cut(s) 13, 314
HphI GGTGA 3 cut(s) 184, 202, 472
Hpy166II GTNNAC 5 cut(s) 58, 82, 192, 199, 289
Hpy188I TCNGA 2 cut(s) 359, 832
Hpy188III TCNNGA 3 cut(s) 17, 181, 605
Hpy8I GTNNAC 5 cut(s) 58, 82, 192, 199, 289
Hpy99I CGWCG 8 cut(s) 92, 104, 110, 200, 203, 287, 363, 526
HpyAV CCTTC 4 cut(s) 43, 181, 409, 442
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 3 cut(s) 54, 78, 291
HpyCH4V TGCA 2 cut(s) 724, 733
HpyF3I CTNAG 2 cut(s) 389, 666
HpySE526I ACGT 3 cut(s) 54, 78, 291
Hsp92I GRCGYC 3 cut(s) 78, 108, 282
Hsp92II CATG 1 cut(s) 337
HspAI GCGC 6 cut(s) 150, 256, 425, 491, 611, 777
Kpn2I TCCGGA 1 cut(s) 604
KpnI GGTACC 2 cut(s) 634, 715
KroI GCCGGC 1 cut(s) 493
KroNI GCCGGC 1 cut(s) 495
LguI GCTCTTC 2 cut(s) 314, 746
LmnI GCTCC 5 cut(s) 4, 19, 25, 304, 454
Lsp1109I GCAGC 6 cut(s) 270, 652, 682, 736, 745, 848
LweI GCATC 1 cut(s) 836
MabI ACCWGGT 1 cut(s) 351
MaeI CTAG 1 cut(s) 788
MaeII ACGT 3 cut(s) 54, 78, 291
MaeIII GTNAC 4 cut(s) 460, 542, 623, 704
MboII GAAGA 5 cut(s) 139, 301, 506, 760, 763
MhlI GDGCHC 3 cut(s) 309, 390, 799
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 1 cut(s) 404
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 2 cut(s) 7, 308
MmeI TCCRAC 2 cut(s) 382, 457
Mox20I TGGCCA 1 cut(s) 443
MreI CGCCGGCG 1 cut(s) 493
MroI TCCGGA 1 cut(s) 604
MroNI GCCGGC 1 cut(s) 493
MscI TGGCCA 1 cut(s) 443
Msp20I TGGCCA 1 cut(s) 443
MvaI CCWGG 2 cut(s) 353, 591
MvnI CGCG 3 cut(s) 150, 425, 491
NaeI GCCGGC 1 cut(s) 495
NciI CCSGG 9 cut(s) 370, 535, 561, 562, 621, 648, 675, 676, 702
NgoMIV GCCGGC 1 cut(s) 493
NlaIII CATG 1 cut(s) 337
NlaIV GGNNCC 5 cut(s) 160, 456, 632, 713, 796
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 314, 746
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 96, 318
PdiI GCCGGC 1 cut(s) 495
Pfl23II CGTACG 1 cut(s) 498
PfoI TCCNGGA 1 cut(s) 368
PleI GAGTC 2 cut(s) 7, 308
PpsI GAGTC 2 cut(s) 7, 308
PpuMI RGGWCCY 1 cut(s) 43
PshAI GACNNNNGTC 1 cut(s) 140
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 309, 390
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 351, 589
PspEI GGTNACC 1 cut(s) 542
PspFI CCCAGC 1 cut(s) 779
PspGI CCWGG 2 cut(s) 351, 589
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 5 cut(s) 160, 456, 632, 713, 796
PspPI GGNCC 3 cut(s) 43, 355, 482
PspPPI RGGWCCY 1 cut(s) 43
RsaI GTAC 7 cut(s) 350, 432, 500, 509, 632, 680, 713
RsaNI GTAC 7 cut(s) 349, 431, 499, 508, 631, 679, 712
SacI GAGCTC 2 cut(s) 309, 390
SalI GTCGAC 1 cut(s) 197
SapI GCTCTTC 2 cut(s) 314, 746
Sau96I GGNCC 3 cut(s) 43, 355, 482
SchI GAGTC 2 cut(s) 7, 308
SduI GDGCHC 3 cut(s) 309, 390, 799
SexAI ACCWGGT 1 cut(s) 351
SfaNI GCATC 1 cut(s) 836
SgrAI CRCCGGYG 2 cut(s) 493, 718
SgrDI CGTCGACG 1 cut(s) 197
SinI GGWCC 3 cut(s) 43, 355, 482
SmaI CCCGGG 2 cut(s) 562, 676
Sse9I AATT 1 cut(s) 404
SspMI CTAG 1 cut(s) 788
SstI GAGCTC 2 cut(s) 309, 390
StyI CCWWGG 3 cut(s) 39, 378, 799
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 3 cut(s) 57, 81, 294
TaqI TCGA 3 cut(s) 99, 198, 312
TasI AATT 1 cut(s) 404
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 6 cut(s) 258, 640, 670, 724, 733, 861
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 2 cut(s) 331, 350
TspGWI ACGGA 2 cut(s) 474, 696
TspMI CCCGGG 2 cut(s) 560, 674
VpaK11BI GGWCC 3 cut(s) 43, 355, 482
XapI RAATTY 1 cut(s) 404
XmaI CCCGGG 2 cut(s) 560, 674
XmiI GTMKAC 2 cut(s) 81, 198
XspI CTAG 1 cut(s) 788
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.