Rw4G019880

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009797
Physical Location & Seq
Reverse (-)
43504031 .. 43504951
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019880.1

Sequence Viewer

Length: 921 bp
ATGGAGCAAAGGACTCTAGAGCTGGAGCTGGTCTCCGCCAAGGACCTGAAGGACGTGATCCTGATATCCAAGATGGACGTCTATGCCGTTGTCTCCCTCGACGGCGACGCCCACTACGGCAAGCAGAAGACCAAGACCAAAGTCGTCCGCGCCTGCGGTACCAACCCCACCTTCAACTTCCCCATGAGGTTCACTATCGACGACACTTTCACCCAACAGAACCGCCTCTCCCTCCAAGTCAAGCTCGTCTGCGAGCGCAGCCTCGGTGACAAGGACATCGGCTTCGTTTTCGTCCCCGTCAAGGAGCTCTTCGACTCCGCCGACCCCAAGAACATGAAGTTCATATCATACCAAGTCCGACGACCCTCCGGGAAGCCCAAGGGCGAGCTCAGCTTCTCTTATAAGTTCGGGGAGAAGGTCACCGTGCCGGTGCCGGAGGTAGGAGGACCGTCTGCCCCAGCGTACGGTGCGTACCCACTTCAAGCGACGAGTTCATCTGGGTCGGGTTACCCGTCGCCTCAACTTGGGTATGGATATGCGCCACCGCCGCCTAGGGCTGGATACCCGGAAGCTCCTCCACGGGGCTACGAGTACCCACCGCAACAACCAGGCTACGCGTACCCTCTGCAGCAGCCCGGGTACGGTTACCCACCGCCGCCGCCGAGTTACGGGTACCGGCCGCAGCAGCCCGGGTACGGGTACCAGCCGGTGCAGCAACCGGAAAAGAAGTTTGAGATGGGATTGAGCGTTGGGTTACAAGGAGGTGCCTTTGGTGGGTTCTTGATTGGAGATGTGATATCCAATGGAGGCGGTGGATGCGGCGGCGGCGGCTACGGCGGTGGCGGTGGATGCGGGGGCGGTGGCGGTGGATGGGGTGGTGGCGACTGCGGCGGTGGCGGTAGCGATTGTGGTGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

306

Amino Acids

32.18

Weight (kDa)

8.07

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 107 1.9e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 3 cut(s) 158, 672, 699
AccB1I GGYRCC 5 cut(s) 158, 430, 672, 699, 764
AccII CGCG 2 cut(s) 150, 617
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 1 cut(s) 677
AcuI CTGAAG 1 cut(s) 68
AcyI GRCGYC 2 cut(s) 78, 108
AfaI GTAC 9 cut(s) 160, 464, 473, 593, 620, 641, 674, 695, 701
AflIII ACRYGT 1 cut(s) 615
AgsI TTSAA 2 cut(s) 175, 482
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
AluBI AGCT 7 cut(s) 22, 28, 244, 307, 388, 393, 572
AluI AGCT 7 cut(s) 22, 28, 244, 307, 388, 393, 572
Alw21I GWGCWC 2 cut(s) 309, 390
Alw26I GTCTC 2 cut(s) 37, 97
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 2 cut(s) 635, 689
AoxI GGCC 1 cut(s) 677
ApeKI GCWGC 6 cut(s) 258, 628, 631, 682, 685, 712
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
Asp718I GGTACC 3 cut(s) 158, 672, 699
AspA2I CCTAGG 1 cut(s) 551
AspLEI GCGC 3 cut(s) 152, 258, 541
AspS9I GGNCC 2 cut(s) 43, 446
AsuC2I CCSGG 6 cut(s) 370, 566, 636, 637, 690, 691
AsuHPI GGTGA 3 cut(s) 202, 278, 412
AvaI CYCGRG 2 cut(s) 635, 689
AvaII GGWCC 2 cut(s) 43, 446
AvrII CCTAGG 1 cut(s) 551
BanI GGYRCC 5 cut(s) 158, 430, 672, 699, 764
BanII GRGCYC 2 cut(s) 309, 390
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 309, 390
BbvI GCAGC 6 cut(s) 270, 640, 643, 694, 697, 724
BccI CCATC 3 cut(s) 67, 730, 864
BceAI ACGGC 4 cut(s) 71, 118, 133, 850
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 554
BcnI CCSGG 6 cut(s) 370, 566, 636, 637, 690, 691
BcoDI GTCTC 2 cut(s) 37, 97
BfaI CTAG 2 cut(s) 17, 552
BfmI CTRYAG 1 cut(s) 626
BfuI GTATCC 1 cut(s) 554
BlnI CCTAGG 1 cut(s) 551
BlpI GCTNAGC 1 cut(s) 389
Bme1390I CCNGG 7 cut(s) 370, 566, 609, 636, 637, 690, 691
Bme18I GGWCC 2 cut(s) 43, 446
BmeT110I CYCGRG 2 cut(s) 635, 689
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 43, 446
BmiI GGNNCC 5 cut(s) 160, 432, 674, 701, 766
BmrFI CCNGG 7 cut(s) 370, 566, 609, 636, 637, 690, 691
BmsI GCATC 2 cut(s) 806, 839
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 1 cut(s) 44
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 6 cut(s) 370, 566, 636, 637, 690, 691
BsaHI GRCGYC 2 cut(s) 78, 108
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 7 cut(s) 39, 262, 378, 551, 578, 635, 689
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse118I RCCGGY 3 cut(s) 427, 675, 706
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 7 cut(s) 39, 262, 378, 551, 578, 635, 689
BseGI GGATG 3 cut(s) 821, 854, 875
BseMII CTCAG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 564
BseX3I CGGCCG 1 cut(s) 677
BseXI GCAGC 6 cut(s) 270, 640, 643, 694, 697, 724
BseYI CCCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 2 cut(s) 150, 617
Bsh1285I CGRYCG 1 cut(s) 680
BshFI GGCC 1 cut(s) 679
BshNI GGYRCC 5 cut(s) 158, 430, 672, 699, 764
BsiEI CGRYCG 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 309, 390
BsiHKCI CYCGRG 2 cut(s) 635, 689
BsiSI CCGG 9 cut(s) 369, 428, 434, 566, 636, 676, 690, 707, 719
BsiWI CGTACG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 37, 97
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 679
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 2 cut(s) 635, 689
Bsp1286I GDGCHC 2 cut(s) 309, 390
Bsp143I GATC 1 cut(s) 57
Bsp1720I GCTNAGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 679
BspCNI CTCAG 1 cut(s) 402
BspFNI CGCG 2 cut(s) 150, 617
BspLI GGNNCC 5 cut(s) 160, 432, 674, 701, 766
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 1 cut(s) 52
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 5 cut(s) 158, 430, 672, 699, 764
BspTNI GGTCTC 1 cut(s) 37
BsrFI RCCGGY 3 cut(s) 427, 675, 706
BssAI RCCGGY 3 cut(s) 427, 675, 706
BssECI CCNNGG 7 cut(s) 39, 262, 378, 551, 578, 635, 689
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 2 cut(s) 78, 108
BssT1I CCWWGG 3 cut(s) 39, 378, 551
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 4 cut(s) 424, 450, 467, 644
Bst6I CTCTTC 1 cut(s) 314
BstACI GRCGYC 2 cut(s) 78, 108
BstC8I GCNNGC 4 cut(s) 122, 154, 254, 386
BstDEI CTNAG 1 cut(s) 389
BstDSI CCRYGG 1 cut(s) 578
BstEII GGTNACC 3 cut(s) 418, 506, 644
BstF5I GGATG 3 cut(s) 821, 854, 875
BstFNI CGCG 2 cut(s) 150, 617
BstHHI GCGC 3 cut(s) 152, 258, 541
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 2 cut(s) 37, 97
BstMBI GATC 1 cut(s) 57
BstMCI CGRYCG 1 cut(s) 680
BstNI CCWGG 1 cut(s) 609
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 3 cut(s) 418, 506, 644
BstSCI CCNGG 7 cut(s) 368, 564, 607, 634, 635, 688, 689
BstSFI CTRYAG 1 cut(s) 626
BstUI CGCG 2 cut(s) 150, 617
BstV1I GCAGC 6 cut(s) 270, 640, 643, 694, 697, 724
BstV2I GAAGAC 1 cut(s) 134
BstZI CGGCCG 1 cut(s) 677
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 1 cut(s) 679
BtgI CCRYGG 1 cut(s) 578
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 3 cut(s) 821, 854, 875
Cac8I GCNNGC 4 cut(s) 122, 154, 254, 386
CfoI GCGC 3 cut(s) 152, 258, 541
Cfr10I RCCGGY 3 cut(s) 427, 675, 706
Cfr13I GGNCC 2 cut(s) 43, 446
Cfr9I CCCGGG 2 cut(s) 635, 689
CseI GACGC 1 cut(s) 116
Csp6I GTAC 9 cut(s) 159, 463, 472, 592, 619, 640, 673, 694, 700
CviAII CATG 2 cut(s) 184, 334
CviQI GTAC 9 cut(s) 159, 463, 472, 592, 619, 640, 673, 694, 700
DdeI CTNAG 1 cut(s) 389
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 677
EagI CGGCCG 1 cut(s) 677
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
EciI GGCGGA 2 cut(s) 25, 307
Ecl136II GAGCTC 2 cut(s) 307, 388
EclXI CGGCCG 1 cut(s) 677
Eco130I CCWWGG 3 cut(s) 39, 378, 551
Eco24I GRGCYC 2 cut(s) 309, 390
Eco31I GGTCTC 1 cut(s) 37
Eco32I GATATC 2 cut(s) 66, 798
Eco47I GGWCC 2 cut(s) 43, 446
Eco52I CGGCCG 1 cut(s) 677
Eco53kI GAGCTC 2 cut(s) 307, 388
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 2 cut(s) 635, 689
Eco91I GGTNACC 3 cut(s) 418, 506, 644
EcoICRI GAGCTC 2 cut(s) 307, 388
EcoO109I RGGNCCY 1 cut(s) 43
EcoO65I GGTNACC 3 cut(s) 418, 506, 644
EcoRII CCWGG 1 cut(s) 607
EcoRV GATATC 2 cut(s) 66, 798
EcoT14I CCWWGG 3 cut(s) 39, 378, 551
EcoT38I GRGCYC 2 cut(s) 309, 390
ErhI CCWWGG 3 cut(s) 39, 378, 551
FaeI CATG 2 cut(s) 187, 337
FaiI YATR 8 cut(s) 84, 185, 335, 344, 349, 402, 531, 537
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 183, 333
FauI CCCGC 1 cut(s) 845
FokI GGATG 3 cut(s) 828, 861, 882
FriOI GRGCYC 2 cut(s) 309, 390
FspBI CTAG 2 cut(s) 17, 552
GlaI GCGC 3 cut(s) 151, 257, 540
GsaI CCCAGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 1 cut(s) 679
HapII CCGG 9 cut(s) 369, 428, 434, 566, 636, 676, 690, 707, 719
HgaI GACGC 1 cut(s) 116
HhaI GCGC 3 cut(s) 152, 258, 541
Hin1I GRCGYC 2 cut(s) 78, 108
Hin1II CATG 2 cut(s) 187, 337
Hin6I GCGC 3 cut(s) 150, 256, 539
HinP1I GCGC 3 cut(s) 150, 256, 539
HinfI GANTC 2 cut(s) 13, 314
HpaII CCGG 9 cut(s) 369, 428, 434, 566, 636, 676, 690, 707, 719
HphI GGTGA 3 cut(s) 202, 278, 412
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 3 cut(s) 17, 61, 781
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 6 cut(s) 104, 110, 203, 363, 490, 517
HpyAV CCTTC 3 cut(s) 43, 181, 409
HpyCH4III ACNGT 4 cut(s) 424, 450, 467, 644
HpyCH4IV ACGT 2 cut(s) 54, 78
HpyCH4V TGCA 2 cut(s) 628, 712
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 2 cut(s) 54, 78
Hsp92I GRCGYC 2 cut(s) 78, 108
Hsp92II CATG 2 cut(s) 187, 337
HspAI GCGC 3 cut(s) 150, 256, 539
KpnI GGTACC 3 cut(s) 162, 676, 703
Kzo9I GATC 1 cut(s) 57
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 4 cut(s) 4, 25, 304, 577
Lsp1109I GCAGC 6 cut(s) 270, 640, 643, 694, 697, 724
LweI GCATC 2 cut(s) 806, 839
MaeI CTAG 2 cut(s) 17, 552
MaeII ACGT 2 cut(s) 54, 78
MaeIII GTNAC 6 cut(s) 266, 418, 506, 644, 665, 753
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 2 cut(s) 139, 301
MhlI GDGCHC 2 cut(s) 309, 390
MluI ACGCGT 1 cut(s) 615
MlyI GAGTC 2 cut(s) 7, 308
MmeI TCCRAC 1 cut(s) 382
MspI CCGG 9 cut(s) 369, 428, 434, 566, 636, 676, 690, 707, 719
MspR9I CCNGG 7 cut(s) 370, 566, 609, 636, 637, 690, 691
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 150, 617
NciI CCSGG 6 cut(s) 370, 566, 636, 637, 690, 691
NdeII GATC 1 cut(s) 57
NlaIII CATG 2 cut(s) 187, 337
NlaIV GGNNCC 5 cut(s) 160, 432, 674, 701, 766
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 2 cut(s) 266, 418
PciSI GCTCTTC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 318, 509
Pfl23II CGTACG 1 cut(s) 462
PfoI TCCNGGA 1 cut(s) 368
PleI GAGTC 2 cut(s) 7, 308
PpsI GAGTC 2 cut(s) 7, 308
PpuMI RGGWCCY 1 cut(s) 43
PshAI GACNNNNGTC 1 cut(s) 140
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 309, 390
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 607
PspEI GGTNACC 3 cut(s) 418, 506, 644
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 607
PspLI CGTACG 1 cut(s) 462
PspN4I GGNNCC 5 cut(s) 160, 432, 674, 701, 766
PspPI GGNCC 2 cut(s) 43, 446
PspPPI RGGWCCY 1 cut(s) 43
PstI CTGCAG 1 cut(s) 630
RsaI GTAC 9 cut(s) 160, 464, 473, 593, 620, 641, 674, 695, 701
RsaNI GTAC 9 cut(s) 159, 463, 472, 592, 619, 640, 673, 694, 700
SacI GAGCTC 2 cut(s) 309, 390
SapI GCTCTTC 1 cut(s) 314
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 2 cut(s) 43, 446
SchI GAGTC 2 cut(s) 7, 308
ScrFI CCNGG 7 cut(s) 370, 566, 609, 636, 637, 690, 691
SduI GDGCHC 2 cut(s) 309, 390
SfaNI GCATC 2 cut(s) 806, 839
SfcI CTRYAG 1 cut(s) 626
SinI GGWCC 2 cut(s) 43, 446
SmaI CCCGGG 2 cut(s) 637, 691
SspMI CTAG 2 cut(s) 17, 552
SstI GAGCTC 2 cut(s) 309, 390
StyD4I CCNGG 7 cut(s) 368, 564, 607, 634, 635, 688, 689
StyI CCWWGG 3 cut(s) 39, 378, 551
TaaI ACNGT 4 cut(s) 424, 450, 467, 644
TaiI ACGT 2 cut(s) 57, 81
TaqI TCGA 3 cut(s) 99, 198, 312
TauI GCSGC 9 cut(s) 550, 658, 661, 682, 822, 825, 828, 831, 891
TseFI GTSAC 2 cut(s) 266, 418
TseI GCWGC 6 cut(s) 258, 628, 631, 682, 685, 712
Tsp45I GTSAC 2 cut(s) 266, 418
TspDTI ATGAA 3 cut(s) 331, 350, 483
TspMI CCCGGG 2 cut(s) 635, 689
VpaK11BI GGWCC 2 cut(s) 43, 446
XbaI TCTAGA 1 cut(s) 16
XmaI CCCGGG 2 cut(s) 635, 689
XmaJI CCTAGG 1 cut(s) 551
XspI CTAG 2 cut(s) 17, 552
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.