MD13G1169700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009797
Physical Location & Seq
Reverse (-)
13728150 .. 13728866
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1169700.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGGGCATATGACACTAGAAGTGGAGCTCATCTCAGCCAAAGACCTCAAAGATGTCAACTTCTTTACCAAGATGTCCGTATACGCCGTCGTCTCCGTCCTCGACGGCGACTCCCAAGAACAGCAGAAAACCAAATCCTTCGTATCCCAAAACTGCGGCACTCATCCCACCTGGAATAACTTTCACATGGACTTCACCGTTGACGAGTGTCTTGCCCAGCAGAGCCGCCTCGCCCTCGTCTTCAAGCTCATCTGCAAGCGCCGCCTCGGCGACAAGTACATTGGCCAAGTGGTTGCTCCCATTAAAGAGCTCTTTGACACCATGGCCAACCCCAACTCCACCATGACGTTCGTGGCTTACCATGTTATTACAAACCCCTCAAGTAACCCCAAAGGTGAACTCCGCTTCTCTTACAAGTTCTGCGATAAAGTGGCGGTTTCACCGGCGGAACCGCCGTTTGCTTGGCCGCAATTCCAACATGAACATACCGCACGGGGGCCACCGTATGTTCCAAGGCAACCTCAATATGAGGATACTGCACTGGCACCGCCTTATGTTCTGCCTCAAACCCAATATGAGTATACTGCAACGGCACCGCCATTTCCTCCCCCACAACCCCGATATAAGTATACTCCATCTGCACCGCCACTATTCGTATACACGTCCCCGGCACCCCGATATCAGTACACTGCATCAGCACCGCCACTGTATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

239

Amino Acids

26.91

Weight (kDa)

7.67

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 107 3e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 544, 592, 670
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 4 cut(s) 81, 581, 629, 657
AcoI YGGCCR 3 cut(s) 283, 324, 464
AfaI GTAC 2 cut(s) 278, 686
AfiI CCNNNNNNNGG 2 cut(s) 495, 710
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 1 cut(s) 244
AjiI CACGTC 1 cut(s) 663
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 3 cut(s) 28, 247, 310
AluI AGCT 3 cut(s) 28, 247, 310
Alw21I GWGCWC 2 cut(s) 30, 312
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 4 cut(s) 283, 324, 464, 497
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 668
AsuHPI GGTGA 3 cut(s) 187, 407, 432
BalI TGGCCA 2 cut(s) 285, 326
BanI GGYRCC 3 cut(s) 544, 592, 670
BanII GRGCYC 2 cut(s) 30, 312
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 2 cut(s) 30, 312
BccI CCATC 1 cut(s) 643
BceAI ACGGC 4 cut(s) 71, 121, 439, 606
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 1 cut(s) 172
BciVI GTATCC 2 cut(s) 154, 526
BcnI CCSGG 1 cut(s) 668
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 262
BfuI GTATCC 2 cut(s) 154, 526
BglI GCCNNNNNGGC 1 cut(s) 267
BisI GCNGC 4 cut(s) 157, 226, 262, 467
BlsI GCNGC 4 cut(s) 158, 227, 263, 468
Bme1390I CCNGG 2 cut(s) 172, 668
BmgBI CACGTC 1 cut(s) 663
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 5 cut(s) 450, 498, 546, 594, 672
BmrFI CCNGG 2 cut(s) 172, 668
BmsI GCATC 1 cut(s) 701
BoxI GACNNNNGTC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpuEI CTTGAG 1 cut(s) 364
BpuMI CCSGG 1 cut(s) 668
BsaJI CCNNGG 4 cut(s) 265, 321, 512, 666
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 710
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 265, 321, 512, 666
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 2 cut(s) 495, 710
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 546
BseYI CCCAGC 1 cut(s) 216
BsgI GTGCAG 2 cut(s) 522, 624
BshFI GGCC 4 cut(s) 285, 326, 466, 499
BshNI GGYRCC 3 cut(s) 544, 592, 670
BsiHKAI GWGCWC 2 cut(s) 30, 312
BsiSI CCGG 2 cut(s) 443, 668
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 2 cut(s) 495, 710
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 4 cut(s) 285, 326, 466, 499
Bsp1286I GDGCHC 2 cut(s) 30, 312
Bsp19I CCATGG 1 cut(s) 321
BspANI GGCC 4 cut(s) 285, 326, 466, 499
BspCNI CTCAG 1 cut(s) 47
BspLI GGNNCC 5 cut(s) 450, 498, 546, 594, 672
BspT107I GGYRCC 3 cut(s) 544, 592, 670
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 1 cut(s) 546
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 4 cut(s) 265, 321, 512, 666
BssNAI GTATAC 4 cut(s) 82, 582, 630, 658
BssT1I CCWWGG 2 cut(s) 321, 512
Bst1107I GTATAC 4 cut(s) 82, 582, 630, 658
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 3 cut(s) 199, 504, 708
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 34
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 1 cut(s) 163
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 1 cut(s) 261
BstMAI GTCTC 1 cut(s) 97
BstMWI GCNNNNNNNGC 2 cut(s) 261, 267
BstNI CCWGG 1 cut(s) 172
BstPAI GACNNNNGTC 1 cut(s) 207
BstSCI CCNGG 2 cut(s) 170, 666
BstV2I GAAGAC 1 cut(s) 232
BstZ17I GTATAC 4 cut(s) 82, 582, 630, 658
BsuI GTATCC 2 cut(s) 154, 526
BsuRI GGCC 4 cut(s) 285, 326, 466, 499
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 663
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 539, 687, 704
Cac8I GCNNGC 1 cut(s) 257
CfoI GCGC 1 cut(s) 261
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 277, 685
CviAII CATG 5 cut(s) 187, 322, 343, 362, 479
CviQI GTAC 2 cut(s) 277, 685
DdeI CTNAG 1 cut(s) 34
EaeI YGGCCR 3 cut(s) 283, 324, 464
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 2 cut(s) 28, 310
Eco130I CCWWGG 2 cut(s) 321, 512
Eco24I GRGCYC 2 cut(s) 30, 312
Eco32I GATATC 1 cut(s) 680
Eco53kI GAGCTC 2 cut(s) 28, 310
EcoICRI GAGCTC 2 cut(s) 28, 310
EcoRII CCWGG 1 cut(s) 170
EcoRV GATATC 1 cut(s) 680
EcoT14I CCWWGG 2 cut(s) 321, 512
EcoT38I GRGCYC 2 cut(s) 30, 312
ErhI CCWWGG 2 cut(s) 321, 512
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 5 cut(s) 190, 325, 346, 365, 482
FaqI GGGAC 1 cut(s) 649
FatI CATG 5 cut(s) 186, 321, 342, 361, 478
FauNDI CATATG 1 cut(s) 9
FblI GTMKAC 4 cut(s) 81, 581, 629, 657
Fnu4HI GCNGC 4 cut(s) 157, 226, 262, 467
FokI GGATG 1 cut(s) 150
FriOI GRGCYC 2 cut(s) 30, 312
Fsp4HI GCNGC 4 cut(s) 157, 226, 262, 467
FspBI CTAG 1 cut(s) 17
GlaI GCGC 1 cut(s) 260
GluI GCNGC 4 cut(s) 157, 226, 262, 467
GsaI CCCAGC 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 4 cut(s) 285, 326, 466, 499
HapII CCGG 2 cut(s) 443, 668
HhaI GCGC 1 cut(s) 261
Hin1II CATG 5 cut(s) 190, 325, 346, 365, 482
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 2 cut(s) 58, 202
HindII GTYRAC 2 cut(s) 58, 202
HinfI GANTC 1 cut(s) 110
HpaII CCGG 2 cut(s) 443, 668
HphI GGTGA 3 cut(s) 187, 407, 432
Hpy166II GTNNAC 8 cut(s) 58, 82, 202, 398, 582, 630, 658, 687
Hpy8I GTNNAC 8 cut(s) 58, 82, 202, 398, 582, 630, 658, 687
Hpy99I CGWCG 2 cut(s) 92, 107
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 3 cut(s) 199, 504, 708
HpyCH4IV ACGT 2 cut(s) 347, 662
HpyCH4V TGCA 5 cut(s) 255, 539, 587, 641, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 267
HpyF3I CTNAG 1 cut(s) 34
HpySE526I ACGT 2 cut(s) 347, 662
Hsp92II CATG 5 cut(s) 190, 325, 346, 365, 482
HspAI GCGC 1 cut(s) 259
LmnI GCTCC 2 cut(s) 25, 301
LpnPI CCDG 6 cut(s) 157, 184, 230, 456, 527, 681
LweI GCATC 1 cut(s) 701
MaeI CTAG 1 cut(s) 17
MaeII ACGT 2 cut(s) 347, 662
MaeIII GTNAC 1 cut(s) 383
MboII GAAGA 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 30, 312
MlsI TGGCCA 2 cut(s) 285, 326
MluCI AATT 1 cut(s) 470
MluNI TGGCCA 2 cut(s) 285, 326
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 499
Mox20I TGGCCA 2 cut(s) 285, 326
MscI TGGCCA 2 cut(s) 285, 326
MseI TTAA 1 cut(s) 303
Msp20I TGGCCA 2 cut(s) 285, 326
MspI CCGG 2 cut(s) 443, 668
MspR9I CCNGG 2 cut(s) 172, 668
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 261, 267
NciI CCSGG 1 cut(s) 668
NcoI CCATGG 1 cut(s) 321
NdeI CATATG 1 cut(s) 9
NlaIII CATG 5 cut(s) 190, 325, 346, 365, 482
NlaIV GGNNCC 5 cut(s) 450, 498, 546, 594, 672
NmeAIII GCCGAG 1 cut(s) 246
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 4 cut(s) 158, 227, 263, 468
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PshAI GACNNNNGTC 1 cut(s) 207
Psp124BI GAGCTC 2 cut(s) 30, 312
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 5 cut(s) 450, 498, 546, 594, 672
PspPI GGNCC 1 cut(s) 497
RsaI GTAC 2 cut(s) 278, 686
RsaNI GTAC 2 cut(s) 277, 685
SacI GAGCTC 2 cut(s) 30, 312
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 4 cut(s) 157, 226, 262, 467
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 172, 668
SduI GDGCHC 2 cut(s) 30, 312
SetI ASST 9 cut(s) 30, 48, 173, 249, 312, 350, 397, 523, 665
SfaNI GCATC 1 cut(s) 701
SgrAI CRCCGGYG 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse9I AATT 1 cut(s) 470
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 2 cut(s) 30, 312
StyD4I CCNGG 2 cut(s) 170, 666
StyI CCWWGG 2 cut(s) 321, 512
TaaI ACNGT 3 cut(s) 199, 504, 708
TaiI ACGT 2 cut(s) 350, 665
TaqI TCGA 1 cut(s) 102
TasI AATT 1 cut(s) 470
TatI WGTACW 2 cut(s) 276, 684
TauI GCSGC 4 cut(s) 159, 228, 264, 469
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 546, 694, 711
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 2 cut(s) 67, 85
TspRI CASTG 3 cut(s) 546, 694, 711
Van91I CCANNNNNTGG 1 cut(s) 710
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XmiI GTMKAC 4 cut(s) 81, 581, 629, 657
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.