MD02G1019900.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009797
Physical Location & Seq
Forward (+)
1422048 .. 1423025
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1019900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGAGCAGAGGACCCTAGAGCTCGAACTCATCTCAGCCAAAGACATCAAAGACGTCAGACTCATTCTCAAGATGGACGTATACGCCGTCGTCTCCCTCCAAGTCGACGACGACTCTCGCGGCAAGCAGCAGAAAACCATGACCAAAGTCGCCCGGGGATGCGGGACCCATCCCACCTGGAACTTCCACGTGAACTTCGTTCTCGACGAGTACCTAACTCAGCAGAACCGCCTCTTCATCGTCTTCAAGCTCGTCTGCCAGCACAGCCACGGCGACAAAGACATCGGCCAGGTCGTCGTCCCCGTTAAGGAGCTTTTTGACTCTGTCAAGGCTGACGCTGCCTCCATGAAGTTCGTCACTTACCAAGTCAGAAAGCCCTCTGGGAAGCCGAAAGGTGAGCTCAATTTTTCCTCCAAATTTGGTGACAAAGTGGCGGCTCCACAGGCAGCGGTGACTGCGGCATCATCGAAGGGTAACGAGCCGGTAACAGCCTATCCGGTGGTTCGACCGTCAGTTCAGCCGATGGTGGGGTACGGTGGATACCCGCCTCCAGTTGGCAGCTTTGCTGGGCCTCCGCCACCTGGATACGAGTACCCGCCTCCTCCACCTGGATACGAGTACCCGCCTCCTCCACCTGGATACGCGTACCCGCCACCGCCACCGGGATATGGATACCCGCCACCGCCACCAGGATACCGCTACCCGCCACCGCCACTGGGATATGGCTACCCGCCACCGCCACCGGGATATGGCTACCCGCCACCGCCACCGGGATATGGCTACCCGCCACCGCCACCGGGGTATGGCTACCCGCCACCGCCACCTCCGGGTTATGGGTACCCCGCGGTGCCGCAAGCCCAACCGGAAGCAAAGAACAAGTTTTGGATGAGACTGGGGGGTGGATTATTAGGGAGAATGCTTACTGGTTTGTGGATTGGAGATATGGTATCTGGTGGTGGTGACAGCGGCGGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

326

Amino Acids

34.95

Weight (kDa)

8.88

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 109 7.4e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 57
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 2 cut(s) 837, 847
AccI GTMKAC 2 cut(s) 81, 105
AccII CGCG 3 cut(s) 120, 644, 845
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 416
AcvI CACGTG 1 cut(s) 190
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 6 cut(s) 212, 533, 593, 620, 647, 839
AflIII ACRYGT 1 cut(s) 642
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 6 cut(s) 176, 288, 580, 607, 634, 688
AluBI AGCT 5 cut(s) 22, 250, 313, 400, 561
AluI AGCT 5 cut(s) 22, 250, 313, 400, 561
Alw21I GWGCWC 2 cut(s) 24, 402
Alw26I GTCTC 2 cut(s) 97, 883
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 2 cut(s) 286, 569
ApeKI GCWGC 4 cut(s) 127, 338, 446, 558
ApoI RAATTY 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 837
AspS9I GGNCC 3 cut(s) 12, 165, 569
AsuC2I CCSGG 7 cut(s) 154, 155, 663, 744, 771, 798, 828
AsuHPI GGTGA 4 cut(s) 407, 434, 463, 971
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 12, 165
BanI GGYRCC 2 cut(s) 837, 847
BanII GRGCYC 2 cut(s) 24, 402
BbrPI CACGTG 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 24, 402
BbvI GCAGC 4 cut(s) 139, 325, 458, 570
BccI CCATC 3 cut(s) 67, 177, 517
BceAI ACGGC 2 cut(s) 71, 286
BciT130I CCWGG 6 cut(s) 178, 290, 582, 609, 636, 690
BciVI GTATCC 6 cut(s) 533, 578, 605, 632, 665, 686
BcnI CCSGG 7 cut(s) 154, 155, 663, 744, 771, 798, 828
BcoDI GTCTC 2 cut(s) 97, 883
BfaI CTAG 1 cut(s) 17
BfuI GTATCC 6 cut(s) 533, 578, 605, 632, 665, 686
BisI GCNGC 9 cut(s) 121, 128, 339, 435, 447, 459, 559, 851, 967
BlsI GCNGC 9 cut(s) 122, 129, 340, 436, 448, 460, 560, 852, 968
Bme18I GGWCC 2 cut(s) 12, 165
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 12, 165, 569
BmiI GGNNCC 6 cut(s) 14, 166, 167, 438, 839, 849
BmrI ACTGGG 2 cut(s) 725, 902
BmsI GCATC 2 cut(s) 149, 470
BmuI ACTGGG 2 cut(s) 725, 902
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 7 cut(s) 154, 155, 663, 744, 771, 798, 828
BsaAI YACGTR 1 cut(s) 190
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 5 cut(s) 153, 154, 268, 797, 843
BsaWI WCCGGW 2 cut(s) 496, 862
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 930, 960
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 4 cut(s) 551, 720, 897, 928
BseBI CCWGG 6 cut(s) 178, 290, 582, 609, 636, 690
BseDI CCNNGG 5 cut(s) 153, 154, 268, 797, 843
BseGI GGATG 3 cut(s) 164, 169, 891
BseMII CTCAG 2 cut(s) 48, 233
BseNI ACTGG 4 cut(s) 551, 720, 897, 928
BseRI GAGGAG 2 cut(s) 591, 618
BseXI GCAGC 4 cut(s) 139, 325, 458, 570
BseYI CCCAGC 1 cut(s) 566
Bsh1236I CGCG 3 cut(s) 120, 644, 845
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 288, 571
BshNI GGYRCC 2 cut(s) 837, 847
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 2 cut(s) 24, 402
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 9 cut(s) 154, 482, 497, 662, 743, 770, 797, 827, 863
BslFI GGGAC 2 cut(s) 178, 284
BsmAI GTCTC 2 cut(s) 97, 883
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 178, 284
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 2 cut(s) 288, 571
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 24, 402
BspANI GGCC 2 cut(s) 288, 571
BspCNI CTCAG 2 cut(s) 47, 232
BspFNI CGCG 3 cut(s) 120, 644, 845
BspLI GGNNCC 6 cut(s) 14, 166, 167, 438, 839, 849
BspT107I GGYRCC 2 cut(s) 837, 847
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 4 cut(s) 551, 720, 897, 928
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 5 cut(s) 153, 154, 268, 797, 843
BssNAI GTATAC 1 cut(s) 82
BssNI GRCGYC 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 82
Bst2UI CCWGG 6 cut(s) 178, 290, 582, 609, 636, 690
Bst4CI ACNGT 2 cut(s) 510, 536
Bst6I CTCTTC 1 cut(s) 239
BstACI GRCGYC 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 125, 260, 855
BstDEI CTNAG 2 cut(s) 34, 219
BstDSI CCRYGG 2 cut(s) 268, 843
BstF5I GGATG 3 cut(s) 164, 169, 891
BstFNI CGCG 3 cut(s) 120, 644, 845
BstMAI GTCTC 2 cut(s) 97, 883
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 4 cut(s) 264, 338, 443, 455
BstNI CCWGG 6 cut(s) 178, 290, 582, 609, 636, 690
BstPAI GACNNNNGTC 1 cut(s) 146
BstUI CGCG 3 cut(s) 120, 644, 845
BstV1I GCAGC 4 cut(s) 139, 325, 458, 570
BstV2I GAAGAC 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 82
BsuI GTATCC 6 cut(s) 533, 578, 605, 632, 665, 686
BsuRI GGCC 2 cut(s) 288, 571
BtgI CCRYGG 2 cut(s) 268, 843
BtsCI GGATG 3 cut(s) 164, 169, 891
BtsIMutI CAGTG 1 cut(s) 713
Cac8I GCNNGC 3 cut(s) 125, 260, 855
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 3 cut(s) 12, 165, 569
Cfr42I CCGCGG 1 cut(s) 846
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 1 cut(s) 344
Csp6I GTAC 6 cut(s) 211, 532, 592, 619, 646, 838
CviAII CATG 2 cut(s) 139, 346
CviQI GTAC 6 cut(s) 211, 532, 592, 619, 646, 838
DdeI CTNAG 2 cut(s) 34, 219
EaeI YGGCCR 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 22, 400
Eco24I GRGCYC 2 cut(s) 24, 402
Eco47I GGWCC 2 cut(s) 12, 165
Eco53kI GAGCTC 2 cut(s) 22, 400
Eco72I CACGTG 1 cut(s) 190
Eco88I CYCGRG 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 22, 400
EcoO109I RGGNCCY 2 cut(s) 12, 165
EcoRII CCWGG 6 cut(s) 176, 288, 580, 607, 634, 688
EcoT38I GRGCYC 2 cut(s) 24, 402
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 142, 349
FaqI GGGAC 2 cut(s) 178, 284
FatI CATG 2 cut(s) 138, 345
FblI GTMKAC 2 cut(s) 81, 105
Fnu4HI GCNGC 9 cut(s) 121, 128, 339, 435, 447, 459, 559, 851, 967
FokI GGATG 3 cut(s) 156, 171, 898
FriOI GRGCYC 2 cut(s) 24, 402
Fsp4HI GCNGC 9 cut(s) 121, 128, 339, 435, 447, 459, 559, 851, 967
FspBI CTAG 1 cut(s) 17
GluI GCNGC 9 cut(s) 121, 128, 339, 435, 447, 459, 559, 851, 967
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 288, 571
HapII CCGG 9 cut(s) 154, 482, 497, 662, 743, 770, 797, 827, 863
HgaI GACGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 2 cut(s) 142, 349
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 3 cut(s) 60, 113, 320
HpaII CCGG 9 cut(s) 154, 482, 497, 662, 743, 770, 797, 827, 863
HphI GGTGA 4 cut(s) 407, 434, 463, 971
Hpy166II GTNNAC 3 cut(s) 82, 106, 193
Hpy188I TCNGA 2 cut(s) 59, 371
Hpy188III TCNNGA 2 cut(s) 70, 203
Hpy8I GTNNAC 3 cut(s) 82, 106, 193
Hpy99I CGWCG 5 cut(s) 92, 110, 113, 209, 299
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 510, 536
HpyCH4IV ACGT 3 cut(s) 54, 78, 189
HpyF10VI GCNNNNNNNGC 4 cut(s) 264, 338, 443, 455
HpyF3I CTNAG 2 cut(s) 34, 219
HpySE526I ACGT 3 cut(s) 54, 78, 189
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 2 cut(s) 142, 349
KflI GGGWCCC 1 cut(s) 165
KpnI GGTACC 1 cut(s) 841
KspI CCGCGG 1 cut(s) 846
LmnI GCTCC 3 cut(s) 4, 310, 442
Lsp1109I GCAGC 4 cut(s) 139, 325, 458, 570
LweI GCATC 2 cut(s) 149, 470
MaeI CTAG 1 cut(s) 17
MaeII ACGT 3 cut(s) 54, 78, 189
MaeIII GTNAC 6 cut(s) 355, 422, 451, 473, 484, 959
MboII GAAGA 2 cut(s) 226, 235
MhlI GDGCHC 2 cut(s) 24, 402
MluCI AATT 2 cut(s) 403, 416
MluI ACGCGT 1 cut(s) 642
MlyI GAGTC 3 cut(s) 54, 107, 314
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 3 cut(s) 449, 845, 966
MspI CCGG 9 cut(s) 154, 482, 497, 662, 743, 770, 797, 827, 863
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 6 cut(s) 178, 290, 582, 609, 636, 690
MvnI CGCG 3 cut(s) 120, 644, 845
MwoI GCNNNNNNNGC 4 cut(s) 264, 338, 443, 455
NciI CCSGG 7 cut(s) 154, 155, 663, 744, 771, 798, 828
NlaIII CATG 2 cut(s) 142, 349
NlaIV GGNNCC 6 cut(s) 14, 166, 167, 438, 839, 849
NmuCI GTSAC 4 cut(s) 355, 422, 451, 959
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 195, 204, 291, 300
PctI GAATGC 1 cut(s) 921
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 9 cut(s) 122, 129, 340, 436, 448, 460, 560, 852, 968
PleI GAGTC 3 cut(s) 54, 107, 314
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
PpsI GAGTC 3 cut(s) 54, 107, 314
Ppu21I YACGTR 1 cut(s) 190
PpuMI RGGWCCY 2 cut(s) 12, 165
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 24, 402
Psp5II RGGWCCY 2 cut(s) 12, 165
Psp6I CCWGG 6 cut(s) 176, 288, 580, 607, 634, 688
PspCI CACGTG 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 6 cut(s) 176, 288, 580, 607, 634, 688
PspN4I GGNNCC 6 cut(s) 14, 166, 167, 438, 839, 849
PspPI GGNCC 3 cut(s) 12, 165, 569
PspPPI RGGWCCY 2 cut(s) 12, 165
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 6 cut(s) 212, 533, 593, 620, 647, 839
RsaNI GTAC 6 cut(s) 211, 532, 592, 619, 646, 838
SacI GAGCTC 2 cut(s) 24, 402
SacII CCGCGG 1 cut(s) 846
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 9 cut(s) 121, 128, 339, 435, 447, 459, 559, 851, 967
Sau96I GGNCC 3 cut(s) 12, 165, 569
SchI GAGTC 3 cut(s) 54, 107, 314
SduI GDGCHC 2 cut(s) 24, 402
SfaNI GCATC 2 cut(s) 149, 470
Sfr303I CCGCGG 1 cut(s) 846
SgrBI CCGCGG 1 cut(s) 846
SinI GGWCC 2 cut(s) 12, 165
SmaI CCCGGG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 2 cut(s) 403, 416
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 2 cut(s) 24, 402
TaaI ACNGT 2 cut(s) 510, 536
TaiI ACGT 3 cut(s) 57, 81, 192
TaqI TCGA 5 cut(s) 24, 105, 204, 467, 505
TasI AATT 2 cut(s) 403, 416
TauI GCSGC 5 cut(s) 123, 437, 461, 853, 969
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 720
TseFI GTSAC 4 cut(s) 355, 422, 451, 959
TseI GCWGC 4 cut(s) 127, 338, 446, 558
Tsp45I GTSAC 4 cut(s) 355, 422, 451, 959
TspDTI ATGAA 2 cut(s) 226, 362
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 720
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 12, 165
XapI RAATTY 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 81, 105
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.