pycom13g14530

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009797
Physical Location & Seq
Reverse (-)
10514297 .. 10515142
846 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14530.1

Sequence Viewer

Length: 846 bp
ATGGAGCAGAGGATCCTAGAACTCGAACTCATCTCAGCCAAAGACATCAAAGACGTCAACCTCATATCCAAGATGGACGTTTACGCCGTCGTCTCCCTCCAGGGCGACGACGTATCTCGCGCCGGCAAGCAGAAAACCAAGACCAAAGTCTCCCAGGGATGCGGGACCCATCCCACCTGGAACTTCCACATGAAGTTCGTTCTCGACGAGTACCTAATTCAGCAGAACCGCATCTCCCTCGTCTTCAAGCTCGTCTGCCAGCGCAGCCTCGGCGACAAAGACATCGGCCAGGTCCTCGTCCCCGTTAAGGAGCTCTTGGATTCCGTTAAGGCTGACGCCGCCTCCATGAAGTTTGTCGCTTACCAAGTCAGAATGCCCTCAGGGAAGCCGAAAGGTGAGCTCGAGTTCTCATACAAATTTGGTGACAAAGTGGCGGCTTCAGAGGCGACGCTGACGGCGGCTTCGTCAAAGGTTAACGAGCCGGTAACGGCCTATCCGGTGGTTGGACCGTCAGTCCAGCCGGTGGTGGGGTATGGTGGATACCCACCTCCAGTTGGCAGCTCTGCCGGGCCTCCGCCACCTGGATACGAGTACCCAGCACCACCGCAGGGATACGGGTACCCGCCGCCGCCACCAGGGTATGGGTACCCGCCACCGCCACCAGGGTATGGGTACCCAGGGCTGCCACCTGCCGGGTATGGATACCAACCGATGCAGCAACCCCAGCCGGAAGCAAAGAAGAACTTTGGGATGGGGTTGGGAGCTGGAGTGTTAGGAGGGTTACTTGGTGGGATGCTAATTGGAGATATGGTATCTGATGGCGGTGGATGTGGTGGCGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

282

Amino Acids

29.67

Weight (kDa)

7.6

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 5 - 108 2.4e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 697
AatII GACGTC 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 697
Acc65I GGTACC 3 cut(s) 618, 645, 672
AccB1I GGYRCC 3 cut(s) 618, 645, 672
AccB7I CCANNNNNTGG 3 cut(s) 523, 641, 668
AccII CGCG 1 cut(s) 120
AclWI GGATC 2 cut(s) 7, 20
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 2 cut(s) 54, 336
AfaI GTAC 5 cut(s) 212, 593, 620, 647, 674
AgsI TTSAA 1 cut(s) 247
AhdI GACNNNNNGTC 1 cut(s) 512
AjnI CCWGG 8 cut(s) 99, 153, 176, 288, 580, 634, 661, 676
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 5 cut(s) 250, 313, 400, 561, 764
AluI AGCT 5 cut(s) 250, 313, 400, 561, 764
Alw21I GWGCWC 2 cut(s) 315, 402
Alw26I GTCTC 2 cut(s) 97, 154
AlwI GGATC 2 cut(s) 7, 20
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 3 cut(s) 286, 489, 569
ApeKI GCWGC 4 cut(s) 264, 558, 682, 715
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
Asp718I GGTACC 3 cut(s) 618, 645, 672
AspLEI GCGC 2 cut(s) 122, 264
AspS9I GGNCC 4 cut(s) 165, 292, 506, 569
AsuC2I CCSGG 2 cut(s) 568, 694
AsuHPI GGTGA 2 cut(s) 407, 434
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 3 cut(s) 165, 292, 506
AxyI CCTNAGG 1 cut(s) 379
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 3 cut(s) 618, 645, 672
BanII GRGCYC 2 cut(s) 315, 402
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 2 cut(s) 315, 402
BbvI GCAGC 4 cut(s) 276, 570, 669, 727
BccI CCATC 4 cut(s) 67, 177, 745, 812
BceAI ACGGC 3 cut(s) 71, 471, 504
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 8 cut(s) 101, 155, 178, 290, 582, 636, 663, 678
BciVI GTATCC 4 cut(s) 533, 578, 605, 695
BcnI CCSGG 2 cut(s) 568, 694
BcoDI GTCTC 2 cut(s) 97, 154
BfaI CTAG 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 4 cut(s) 533, 578, 605, 695
BisI GCNGC 9 cut(s) 265, 339, 435, 459, 559, 626, 629, 683, 716
BlsI GCNGC 9 cut(s) 266, 340, 436, 460, 560, 627, 630, 684, 717
Bme18I GGWCC 3 cut(s) 165, 292, 506
BmeRI GACNNNNNGTC 1 cut(s) 512
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 4 cut(s) 165, 292, 506, 569
BmiI GGNNCC 6 cut(s) 14, 166, 167, 620, 647, 674
BmsI GCATC 4 cut(s) 149, 240, 702, 783
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 3 cut(s) 83, 534, 786
BpuMI CCSGG 2 cut(s) 568, 694
BsaHI GRCGYC 2 cut(s) 54, 336
BsaJI CCNNGG 8 cut(s) 100, 153, 154, 268, 635, 662, 676, 677
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 8 cut(s) 218, 248, 326, 356, 753, 783, 795, 825
Bse118I RCCGGY 3 cut(s) 122, 481, 520
Bse1I ACTGG 1 cut(s) 551
Bse21I CCTNAGG 1 cut(s) 379
BseBI CCWGG 8 cut(s) 101, 155, 178, 290, 582, 636, 663, 678
BseDI CCNNGG 8 cut(s) 100, 153, 154, 268, 635, 662, 676, 677
BseGI GGATG 5 cut(s) 164, 169, 756, 798, 833
BseMII CTCAG 2 cut(s) 48, 393
BseNI ACTGG 1 cut(s) 551
BseXI GCAGC 4 cut(s) 276, 570, 669, 727
BseYI CCCAGC 2 cut(s) 595, 723
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 3 cut(s) 288, 491, 571
BshNI GGYRCC 3 cut(s) 618, 645, 672
BsiHKAI GWGCWC 2 cut(s) 315, 402
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 7 cut(s) 123, 482, 497, 521, 567, 693, 728
BslFI GGGAC 2 cut(s) 178, 284
BsmAI GTCTC 2 cut(s) 97, 154
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 2 cut(s) 178, 284
BsmI GAATGC 1 cut(s) 378
BsnI GGCC 3 cut(s) 288, 491, 571
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 315, 402
Bsp143I GATC 1 cut(s) 12
BspANI GGCC 3 cut(s) 288, 491, 571
BspCNI CTCAG 2 cut(s) 47, 392
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 6 cut(s) 14, 166, 167, 620, 647, 674
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 2 cut(s) 7, 20
BspT107I GGYRCC 3 cut(s) 618, 645, 672
BsrFI RCCGGY 3 cut(s) 122, 481, 520
BsrI ACTGG 1 cut(s) 551
BssAI RCCGGY 3 cut(s) 122, 481, 520
BssECI CCNNGG 8 cut(s) 100, 153, 154, 268, 635, 662, 676, 677
BssMI GATC 1 cut(s) 12
BssNI GRCGYC 2 cut(s) 54, 336
Bst2UI CCWGG 8 cut(s) 101, 155, 178, 290, 582, 636, 663, 678
Bst4CI ACNGT 1 cut(s) 510
BstACI GRCGYC 2 cut(s) 54, 336
BstC8I GCNNGC 3 cut(s) 124, 128, 260
BstDEI CTNAG 2 cut(s) 34, 379
BstF5I GGATG 5 cut(s) 164, 169, 756, 798, 833
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 2 cut(s) 122, 264
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 2 cut(s) 97, 154
BstMBI GATC 1 cut(s) 12
BstMWI GCNNNNNNNGC 5 cut(s) 264, 270, 338, 443, 724
BstNI CCWGG 8 cut(s) 101, 155, 178, 290, 582, 636, 663, 678
BstPAI GACNNNNGTC 1 cut(s) 146
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 276, 570, 669, 727
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
Bsu36I CCTNAGG 1 cut(s) 379
BsuI GTATCC 4 cut(s) 533, 578, 605, 695
BsuRI GGCC 3 cut(s) 288, 491, 571
BtsCI GGATG 5 cut(s) 164, 169, 756, 798, 833
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 3 cut(s) 124, 128, 260
CfoI GCGC 2 cut(s) 122, 264
Cfr10I RCCGGY 3 cut(s) 122, 481, 520
Cfr13I GGNCC 4 cut(s) 165, 292, 506, 569
CseI GACGC 2 cut(s) 344, 457
Csp6I GTAC 5 cut(s) 211, 592, 619, 646, 673
CviAII CATG 2 cut(s) 190, 346
CviQI GTAC 5 cut(s) 211, 592, 619, 646, 673
DdeI CTNAG 2 cut(s) 34, 379
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 512
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 2 cut(s) 313, 400
Eco24I GRGCYC 2 cut(s) 315, 402
Eco47I GGWCC 3 cut(s) 165, 292, 506
Eco53kI GAGCTC 2 cut(s) 313, 400
Eco57I CTGAAG 1 cut(s) 423
Eco81I CCTNAGG 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 313, 400
EcoO109I RGGNCCY 2 cut(s) 165, 292
EcoRII CCWGG 8 cut(s) 99, 153, 176, 288, 580, 634, 661, 676
EcoT38I GRGCYC 2 cut(s) 315, 402
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 193, 349
FaiI YATR 9 cut(s) 65, 191, 347, 412, 534, 642, 669, 699, 809
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 728, 760
FaqI GGGAC 2 cut(s) 178, 284
FatI CATG 2 cut(s) 189, 345
FauI CCCGC 3 cut(s) 155, 630, 657
Fnu4HI GCNGC 9 cut(s) 265, 339, 435, 459, 559, 626, 629, 683, 716
FokI GGATG 5 cut(s) 156, 171, 763, 805, 840
FriOI GRGCYC 2 cut(s) 315, 402
Fsp4HI GCNGC 9 cut(s) 265, 339, 435, 459, 559, 626, 629, 683, 716
FspBI CTAG 1 cut(s) 17
GlaI GCGC 2 cut(s) 121, 263
GluI GCNGC 9 cut(s) 265, 339, 435, 459, 559, 626, 629, 683, 716
GsaI CCCAGC 2 cut(s) 599, 727
GsuI CTGGAG 3 cut(s) 83, 534, 786
HaeIII GGCC 3 cut(s) 288, 491, 571
HapII CCGG 7 cut(s) 123, 482, 497, 521, 567, 693, 728
HgaI GACGC 2 cut(s) 344, 457
HhaI GCGC 2 cut(s) 122, 264
Hin1I GRCGYC 2 cut(s) 54, 336
Hin1II CATG 2 cut(s) 193, 349
Hin6I GCGC 2 cut(s) 120, 262
HinP1I GCGC 2 cut(s) 120, 262
HincII GTYRAC 2 cut(s) 58, 475
HindII GTYRAC 2 cut(s) 58, 475
HinfI GANTC 1 cut(s) 320
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 7 cut(s) 123, 482, 497, 521, 567, 693, 728
HphI GGTGA 2 cut(s) 407, 434
Hpy166II GTNNAC 3 cut(s) 58, 82, 475
Hpy188I TCNGA 3 cut(s) 371, 442, 817
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 3 cut(s) 58, 82, 475
Hpy99I CGWCG 5 cut(s) 92, 110, 113, 209, 451
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4IV ACGT 3 cut(s) 54, 78, 111
HpyCH4V TGCA 1 cut(s) 715
HpyF10VI GCNNNNNNNGC 5 cut(s) 264, 270, 338, 443, 724
HpyF3I CTNAG 2 cut(s) 34, 379
HpySE526I ACGT 3 cut(s) 54, 78, 111
Hsp92I GRCGYC 2 cut(s) 54, 336
Hsp92II CATG 2 cut(s) 193, 349
HspAI GCGC 2 cut(s) 120, 262
KflI GGGWCCC 1 cut(s) 165
KpnI GGTACC 3 cut(s) 622, 649, 676
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 4, 310, 761
Lsp1109I GCAGC 4 cut(s) 276, 570, 669, 727
LweI GCATC 4 cut(s) 149, 240, 702, 783
MaeI CTAG 1 cut(s) 17
MaeII ACGT 3 cut(s) 54, 78, 111
MaeIII GTNAC 3 cut(s) 422, 484, 780
MalI GATC 1 cut(s) 14
MboI GATC 1 cut(s) 12
MboII GAAGA 2 cut(s) 235, 751
MflI RGATCY 1 cut(s) 12
MhlI GDGCHC 2 cut(s) 315, 402
MluCI AATT 3 cut(s) 216, 416, 798
MmeI TCCRAC 1 cut(s) 484
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 3 cut(s) 306, 327, 474
MspI CCGG 7 cut(s) 123, 482, 497, 521, 567, 693, 728
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 8 cut(s) 101, 155, 178, 290, 582, 636, 663, 678
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 5 cut(s) 264, 270, 338, 443, 724
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 2 cut(s) 568, 694
NdeII GATC 1 cut(s) 12
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 2 cut(s) 193, 349
NlaIV GGNNCC 6 cut(s) 14, 166, 167, 620, 647, 674
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 401
PaqCI CACCTGC 1 cut(s) 697
PasI CCCWGGG 2 cut(s) 154, 677
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 204, 461
PctI GAATGC 1 cut(s) 378
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 320
PflMI CCANNNNNTGG 3 cut(s) 523, 641, 668
PkrI GCNGC 9 cut(s) 266, 340, 436, 460, 560, 627, 630, 684, 717
PpuMI RGGWCCY 2 cut(s) 165, 292
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 2 cut(s) 315, 402
Psp5II RGGWCCY 2 cut(s) 165, 292
Psp6I CCWGG 8 cut(s) 99, 153, 176, 288, 580, 634, 661, 676
PspFI CCCAGC 2 cut(s) 595, 723
PspGI CCWGG 8 cut(s) 99, 153, 176, 288, 580, 634, 661, 676
PspN4I GGNNCC 6 cut(s) 14, 166, 167, 620, 647, 674
PspPI GGNCC 4 cut(s) 165, 292, 506, 569
PspPPI RGGWCCY 2 cut(s) 165, 292
PspXI VCTCGAGB 1 cut(s) 401
PsuI RGATCY 1 cut(s) 12
RsaI GTAC 5 cut(s) 212, 593, 620, 647, 674
RsaNI GTAC 5 cut(s) 211, 592, 619, 646, 673
SacI GAGCTC 2 cut(s) 315, 402
SaqAI TTAA 3 cut(s) 306, 327, 474
SatI GCNGC 9 cut(s) 265, 339, 435, 459, 559, 626, 629, 683, 716
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 4 cut(s) 165, 292, 506, 569
SduI GDGCHC 2 cut(s) 315, 402
SfaNI GCATC 4 cut(s) 149, 240, 702, 783
Sfr274I CTCGAG 1 cut(s) 401
SinI GGWCC 3 cut(s) 165, 292, 506
SlaI CTCGAG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 3 cut(s) 216, 416, 798
SspMI CTAG 1 cut(s) 17
SstI GAGCTC 2 cut(s) 315, 402
TaaI ACNGT 1 cut(s) 510
TaiI ACGT 3 cut(s) 57, 81, 114
TaqI TCGA 3 cut(s) 24, 204, 402
TasI AATT 3 cut(s) 216, 416, 798
TauI GCSGC 5 cut(s) 341, 437, 461, 628, 631
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 3 cut(s) 306, 327, 474
Tru9I TTAA 3 cut(s) 306, 327, 474
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 4 cut(s) 264, 558, 682, 715
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 206, 362
TspGWI ACGGA 1 cut(s) 313
Van91I CCANNNNNTGG 3 cut(s) 523, 641, 668
VpaK11BI GGWCC 3 cut(s) 165, 292, 506
XapI RAATTY 1 cut(s) 416
XhoI CTCGAG 1 cut(s) 401
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.