Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Forward (+)

6261733 .. 6264361

Gene Structure 2629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G18270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1233 bp

ATGTTTTCGATTTTATCATCATCTTCTTCTTCACAACTCGTCAATTCAACTCTCAATTTCCAAAATTCGCCGAAATCTCCGATCACCGTTATGTCGAAGACGTCTAAATTCAAAACCCTGACGGAGAATTTCACCGTGAGAGTGCTTAAAGCAGAGAATCGCGAACTGAACGTGGCTCTGCTCTCACCTTTCACTATCGCTTCTTCACGGCTTGATTCTGTCAGTAATGTTGCGATTCGGATTGAGCTCAACGATGGATTTGTGGGTTGGGGAGAAGCTCCGATTTTGCCGTCGGTAACGGCGGAGGATCAGATAATGGCTATGGTAAAAGCGCGGGAAGCTAGTGAGTTTCTGAGAGAGTTACCGGAGATGAAACTCGGTAATGTTCTTCAGGAGATCGGAAGATTTCTTCCTGGTCATCAGTTTGCTTCTGTAAGAGCTGGGATGGAGATGGCTATGATTGATGCAGCTGCTAAGAGTGTAAGAGTGCCATTGTGGAAACTATTTGGTGGAGCTTCAAGTACGATTACTACAGACATAACAATTCCGATAGTTTCTCCGGCTGAAGCTTCCGTATTGGCCTCAAAGTATAGAAAACGAGGTTTTGAAACCTTGAAGCTCAAGGTGGGAAAGAATCTTAAGGCTGACATAGAAGTTCTCCAGGCTATACGTGCAGTCCACCCTACTTGTTCCTTCATTTTGGATGCCAATGAGGGTTATCAAACAGAGGAAGCTGTTAAAGTTCTCGAGACGCTGCATGAAATGAAGGTTACACCGGTTCTCTTTGAACAACCTGTCCACAGAGACAATTGGGAGGGTCTCAGTCACGTGACTCGAACTGCAAAGAACAGATTCGGAGTCTCTATTGCAGCAGATGAGAGTTGCAGAGGCTTGACTGATCTCAAAAAAATCATAGAAGGTAATATCGTAGATGTTGTGAACATCAAACTCGCCAAAACCGGAATCCTCGAGTCTCTTGAGGTAATTGAATTGGCTAGATCATCTGGCATTGAGCTTATGATAGGGGGAATGGTGGAGACTAGATTAGCAATGGGGTTCTCTGGTCACCTTGCTGCTGGCCTTGGATGCTTCAGATTCATCGACTTGGACACTCCGCTTCTTCTGGCTGATGATCCAGTTCAAGGTGGCTACAAAGCATGTGGTGCTGTCTATGAGTTTAAAGATGAAGGCGGTCACGGAGGATATCTTCAGTGGAATGATGTTGCTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

410

Amino Acids

44.66

Weight (kDa)

5.91

Isoelectric Point (pI)

38.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	59 - 170	5.7e-10	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	194 - 400	8.7e-34	Enolase C-terminal domain-like
MAAL_C	PF07476	241 - 350	1.5e-07	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	104
AccII	CGCG	2 cut(s)	162, 334
AciI	CCGC	4 cut(s)	302, 334, 1115, 1191
AclWI	GGATC	2 cut(s)	315, 1127
AcsI	RAATTY	3 cut(s)	64, 107, 127
AcuI	CTGAAG	4 cut(s)	374, 585, 1075, 1193
AcvI	CACGTG	1 cut(s)	829
AcyI	GRCGYC	1 cut(s)	101
AfaI	GTAC	1 cut(s)	523
AfiI	CCNNNNNNNGG	1 cut(s)	1142
AflII	CTTAAG	1 cut(s)	638
AgeI	ACCGGT	1 cut(s)	775
AgsI	TTSAA	8 cut(s)	48, 112, 519, 608, 616, 788, 989, 1142
AjnI	CCWGG	2 cut(s)	412, 660
AloI	GAACNNNNNNTCC	2 cut(s)	780, 812
Alw21I	GWGCWC	1 cut(s)	249
Alw26I	GTCTC	6 cut(s)	743, 798, 824, 865, 978, 1031
AlwI	GGATC	2 cut(s)	315, 1127
Ama87I	CYCGRG	2 cut(s)	746, 968
AoxI	GGCC	2 cut(s)	579, 1078
ApeKI	GCWGC	5 cut(s)	467, 470, 754, 869, 1073
ApoI	RAATTY	3 cut(s)	64, 107, 127
AsiGI	ACCGGT	1 cut(s)	775
Asp700I	GAANNNNTTC	1 cut(s)	851
AspLEI	GCGC	1 cut(s)	334
AsuHPI	GGTGA	4 cut(s)	76, 124, 177, 1058
AvaI	CYCGRG	2 cut(s)	746, 968
BanII	GRGCYC	1 cut(s)	249
BbrPI	CACGTG	1 cut(s)	829
BbsI	GAAGAC	1 cut(s)	104
Bbv12I	GWGCWC	1 cut(s)	249
BbvI	GCAGC	5 cut(s)	457, 479, 741, 881, 1060
BccI	CCATC	3 cut(s)	248, 439, 445
BceAI	ACGGC	3 cut(s)	224, 274, 315
BciT130I	CCWGG	2 cut(s)	414, 662
BcoDI	GTCTC	6 cut(s)	743, 798, 824, 865, 978, 1031
BfaI	CTAG	3 cut(s)	342, 996, 1041
BfmI	CTRYAG	1 cut(s)	531
BfrI	CTTAAG	1 cut(s)	638
BisI	GCNGC	5 cut(s)	468, 471, 755, 870, 1074
BlsI	GCNGC	5 cut(s)	469, 472, 756, 871, 1075
Bme1390I	CCNGG	2 cut(s)	414, 662
BmeT110I	CYCGRG	2 cut(s)	746, 968
BmrFI	CCNGG	2 cut(s)	414, 662
BmsI	GCATC	3 cut(s)	454, 694, 1076
BpiI	GAAGAC	1 cut(s)	104
BpmI	CTGGAG	1 cut(s)	644
BpuEI	CTTGAG	2 cut(s)	605, 998
BsaAI	YACGTR	2 cut(s)	671, 829
BsaHI	GRCGYC	1 cut(s)	101
BsaI	GGTCTC	1 cut(s)	824
BsaJI	CCNNGG	1 cut(s)	1081
BsaWI	WCCGGW	3 cut(s)	364, 775, 959
Bsc4I	CCNNNNNNNGG	1 cut(s)	1142
Bse118I	RCCGGY	1 cut(s)	775
Bse1I	ACTGG	1 cut(s)	1136
Bse3DI	GCAATG	1 cut(s)	1056
BseBI	CCWGG	2 cut(s)	414, 662
BseDI	CCNNGG	1 cut(s)	1081
BseGI	GGATG	3 cut(s)	450, 709, 1091
BseLI	CCNNNNNNNGG	1 cut(s)	1142
BseMI	GCAATG	1 cut(s)	1056
BseMII	CTCAG	2 cut(s)	344, 835
BseNI	ACTGG	1 cut(s)	1136
BseXI	GCAGC	5 cut(s)	457, 479, 741, 881, 1060
BseYI	CCCAGC	1 cut(s)	440
BsgI	GTGCAG	1 cut(s)	693
Bsh1236I	CGCG	2 cut(s)	162, 334
BshFI	GGCC	2 cut(s)	581, 1080
BshTI	ACCGGT	1 cut(s)	775
BsiHKAI	GWGCWC	1 cut(s)	249
BsiHKCI	CYCGRG	2 cut(s)	746, 968
BsiSI	CCGG	4 cut(s)	365, 560, 776, 960
BslI	CCNNNNNNNGG	1 cut(s)	1142
BsmAI	GTCTC	6 cut(s)	743, 798, 824, 865, 978, 1031
BsmBI	CGTCTC	1 cut(s)	743
BsnI	GGCC	2 cut(s)	581, 1080
Bso31I	GGTCTC	1 cut(s)	824
BsoBI	CYCGRG	2 cut(s)	746, 968
Bsp1286I	GDGCHC	1 cut(s)	249
Bsp143I	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
Bsp68I	TCGCGA	1 cut(s)	162
BspACI	CCGC	4 cut(s)	302, 334, 1115, 1191
BspANI	GGCC	2 cut(s)	581, 1080
BspCNI	CTCAG	2 cut(s)	345, 834
BspFNI	CGCG	2 cut(s)	162, 334
BspPI	GGATC	2 cut(s)	315, 1127
BspTI	CTTAAG	1 cut(s)	638
BspTNI	GGTCTC	1 cut(s)	824
BsrDI	GCAATG	1 cut(s)	1056
BsrFI	RCCGGY	1 cut(s)	775
BsrI	ACTGG	1 cut(s)	1136
BssAI	RCCGGY	1 cut(s)	775
BssECI	CCNNGG	1 cut(s)	1081
BssMI	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
BssNI	GRCGYC	1 cut(s)	101
BssT1I	CCWWGG	1 cut(s)	1081
Bst2UI	CCWGG	2 cut(s)	414, 662
Bst4CI	ACNGT	2 cut(s)	88, 136
BstACI	GRCGYC	1 cut(s)	101
BstAFI	CTTAAG	1 cut(s)	638
BstAPI	GCANNNNNTGC	1 cut(s)	1163
BstBAI	YACGTR	2 cut(s)	671, 829
BstC8I	GCNNGC	1 cut(s)	1078
BstDEI	CTNAG	4 cut(s)	353, 474, 821, 1227
BstEII	GGTNACC	1 cut(s)	1064
BstF5I	GGATG	3 cut(s)	450, 709, 1091
BstFNI	CGCG	2 cut(s)	162, 334
BstHHI	GCGC	1 cut(s)	334
BstKTI	GATC	6 cut(s)	84, 310, 399, 901, 1001, 1135
BstMAI	GTCTC	6 cut(s)	743, 798, 824, 865, 978, 1031
BstMBI	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
BstMWI	GCNNNNNNNGC	4 cut(s)	338, 671, 1086, 1163
BstNI	CCWGG	2 cut(s)	414, 662
BstNSI	RCATGY	1 cut(s)	1161
BstPI	GGTNACC	1 cut(s)	1064
BstSCI	CCNGG	2 cut(s)	412, 660
BstSFI	CTRYAG	1 cut(s)	531
BstUI	CGCG	2 cut(s)	162, 334
BstV1I	GCAGC	5 cut(s)	457, 479, 741, 881, 1060
BstV2I	GAAGAC	1 cut(s)	104
BsuRI	GGCC	2 cut(s)	581, 1080
BtsCI	GGATG	3 cut(s)	450, 709, 1091
BtsIMutI	CAGTG	1 cut(s)	1217
BtuMI	TCGCGA	1 cut(s)	162
Cac8I	GCNNGC	1 cut(s)	1078
CfoI	GCGC	1 cut(s)	334
Cfr10I	RCCGGY	1 cut(s)	775
CseI	GACGC	1 cut(s)	760
Csp6I	GTAC	1 cut(s)	522
CspAI	ACCGGT	1 cut(s)	775
CspCI	CAANNNNNGTGG	4 cut(s)	668, 703, 1141, 1176
CviAII	CATG	2 cut(s)	758, 1158
CviQI	GTAC	1 cut(s)	522
DdeI	CTNAG	4 cut(s)	353, 474, 821, 1227
DpnI	GATC	6 cut(s)	83, 309, 398, 900, 1000, 1134
DpnII	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
DraI	TTTAAA	1 cut(s)	1180
EciI	GGCGGA	1 cut(s)	317
Ecl136II	GAGCTC	1 cut(s)	247
Eco130I	CCWWGG	1 cut(s)	1081
Eco24I	GRGCYC	1 cut(s)	249
Eco31I	GGTCTC	1 cut(s)	824
Eco32I	GATATC	1 cut(s)	1205
Eco53kI	GAGCTC	1 cut(s)	247
Eco57I	CTGAAG	4 cut(s)	374, 585, 1075, 1193
Eco72I	CACGTG	1 cut(s)	829
Eco88I	CYCGRG	2 cut(s)	746, 968
Eco91I	GGTNACC	1 cut(s)	1064
EcoICRI	GAGCTC	1 cut(s)	247
EcoO65I	GGTNACC	1 cut(s)	1064
EcoRII	CCWGG	2 cut(s)	412, 660
EcoRV	GATATC	1 cut(s)	1205
EcoT14I	CCWWGG	1 cut(s)	1081
EcoT38I	GRGCYC	1 cut(s)	249
ErhI	CCWWGG	1 cut(s)	1081
Esp3I	CGTCTC	1 cut(s)	743
FaeI	CATG	2 cut(s)	761, 1161
FatI	CATG	2 cut(s)	757, 1157
FauI	CCCGC	1 cut(s)	327
Fnu4HI	GCNGC	5 cut(s)	468, 471, 755, 870, 1074
FokI	GGATG	3 cut(s)	457, 716, 1098
FriOI	GRGCYC	1 cut(s)	249
Fsp4HI	GCNGC	5 cut(s)	468, 471, 755, 870, 1074
FspBI	CTAG	3 cut(s)	342, 996, 1041
GlaI	GCGC	1 cut(s)	333
GluI	GCNGC	5 cut(s)	468, 471, 755, 870, 1074
GsaI	CCCAGC	1 cut(s)	444
GsuI	CTGGAG	1 cut(s)	644
HaeIII	GGCC	2 cut(s)	581, 1080
HapII	CCGG	4 cut(s)	365, 560, 776, 960
HgaI	GACGC	1 cut(s)	760
HhaI	GCGC	1 cut(s)	334
Hin1I	GRCGYC	1 cut(s)	101
Hin1II	CATG	2 cut(s)	761, 1161
Hin6I	GCGC	1 cut(s)	332
HinP1I	GCGC	1 cut(s)	332
HindIII	AAGCTT	1 cut(s)	567
HpaII	CCGG	4 cut(s)	365, 560, 776, 960
HphI	GGTGA	4 cut(s)	76, 124, 177, 1058
Hpy166II	GTNNAC	3 cut(s)	679, 799, 940
Hpy188I	TCNGA	9 cut(s)	81, 240, 282, 312, 354, 401, 549, 857, 1094
Hpy188III	TCNNGA	5 cut(s)	161, 392, 746, 748, 977
Hpy8I	GTNNAC	3 cut(s)	679, 799, 940
Hpy99I	CGWCG	1 cut(s)	295
HpyAV	CCTTC	4 cut(s)	703, 760, 911, 1181
HpyCH4III	ACNGT	2 cut(s)	88, 136
HpyCH4IV	ACGT	4 cut(s)	101, 171, 670, 828
HpyCH4V	TGCA	6 cut(s)	467, 674, 757, 842, 869, 885
HpyF10VI	GCNNNNNNNGC	4 cut(s)	338, 671, 1086, 1163
HpyF3I	CTNAG	4 cut(s)	353, 474, 821, 1227
HpySE526I	ACGT	4 cut(s)	101, 171, 670, 828
Hsp92I	GRCGYC	1 cut(s)	101
Hsp92II	CATG	2 cut(s)	761, 1161
HspAI	GCGC	1 cut(s)	332
Kzo9I	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
LmnI	GCTCC	2 cut(s)	283, 512
Lsp1109I	GCAGC	5 cut(s)	457, 479, 741, 881, 1060
LweI	GCATC	3 cut(s)	454, 694, 1076
MaeI	CTAG	3 cut(s)	342, 996, 1041
MaeII	ACGT	4 cut(s)	101, 171, 670, 828
MaeIII	GTNAC	7 cut(s)	295, 360, 769, 824, 829, 1064, 1193
MalI	GATC	6 cut(s)	83, 309, 398, 900, 1000, 1134
MboI	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
MfeI	CAATTG	1 cut(s)	808
MhlI	GDGCHC	1 cut(s)	249
MluCI	AATT	9 cut(s)	43, 55, 64, 107, 127, 543, 808, 984, 989
MlyI	GAGTC	3 cut(s)	826, 867, 980
MroXI	GAANNNNTTC	1 cut(s)	851
MseI	TTAA	4 cut(s)	147, 639, 738, 1179
MslI	CAYNNNNRTG	1 cut(s)	89
MspA1I	CMGCKG	1 cut(s)	470
MspCI	CTTAAG	1 cut(s)	638
MspI	CCGG	4 cut(s)	365, 560, 776, 960
MspR9I	CCNGG	2 cut(s)	414, 662
MunI	CAATTG	1 cut(s)	808
MvaI	CCWGG	2 cut(s)	414, 662
MvnI	CGCG	2 cut(s)	162, 334
MwoI	GCNNNNNNNGC	4 cut(s)	338, 671, 1086, 1163
NdeII	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
NlaIII	CATG	2 cut(s)	761, 1161
NmuCI	GTSAC	4 cut(s)	824, 829, 1064, 1193
NruI	TCGCGA	1 cut(s)	162
NspI	RCATGY	1 cut(s)	1161
PaeR7I	CTCGAG	2 cut(s)	746, 968
PdmI	GAANNNNTTC	1 cut(s)	851
PfeI	GAWTC	7 cut(s)	157, 215, 235, 634, 852, 963, 1095
PinAI	ACCGGT	1 cut(s)	775
PkrI	GCNGC	5 cut(s)	469, 472, 756, 871, 1075
PleI	GAGTC	3 cut(s)	826, 866, 979
PmaCI	CACGTG	1 cut(s)	829
PmlI	CACGTG	1 cut(s)	829
PpsI	GAGTC	3 cut(s)	826, 866, 979
Ppu21I	YACGTR	2 cut(s)	671, 829
Psp124BI	GAGCTC	1 cut(s)	249
Psp6I	CCWGG	2 cut(s)	412, 660
PspCI	CACGTG	1 cut(s)	829
PspEI	GGTNACC	1 cut(s)	1064
PspFI	CCCAGC	1 cut(s)	440
PspGI	CCWGG	2 cut(s)	412, 660
PspXI	VCTCGAGB	1 cut(s)	968
PvuII	CAGCTG	1 cut(s)	470
RruI	TCGCGA	1 cut(s)	162
RsaI	GTAC	1 cut(s)	523
RsaNI	GTAC	1 cut(s)	522
RseI	CAYNNNNRTG	1 cut(s)	89
SacI	GAGCTC	1 cut(s)	249
SaqAI	TTAA	4 cut(s)	147, 639, 738, 1179
SatI	GCNGC	5 cut(s)	468, 471, 755, 870, 1074
Sau3AI	GATC	6 cut(s)	81, 307, 396, 898, 998, 1132
SchI	GAGTC	3 cut(s)	826, 867, 980
ScrFI	CCNGG	2 cut(s)	414, 662
SduI	GDGCHC	1 cut(s)	249
SfaNI	GCATC	3 cut(s)	454, 694, 1076
SfcI	CTRYAG	1 cut(s)	531
Sfr274I	CTCGAG	2 cut(s)	746, 968
SlaI	CTCGAG	2 cut(s)	746, 968
SmiMI	CAYNNNNRTG	1 cut(s)	89
SmlI	CTYRAG	5 cut(s)	620, 638, 746, 968, 977
SmoI	CTYRAG	5 cut(s)	620, 638, 746, 968, 977
Sse9I	AATT	9 cut(s)	43, 55, 64, 107, 127, 543, 808, 984, 989
SsiI	CCGC	4 cut(s)	302, 334, 1115, 1191
SspMI	CTAG	3 cut(s)	342, 996, 1041
SstI	GAGCTC	1 cut(s)	249
StyD4I	CCNGG	2 cut(s)	412, 660
StyI	CCWWGG	1 cut(s)	1081
TaaI	ACNGT	2 cut(s)	88, 136
TaiI	ACGT	4 cut(s)	104, 174, 673, 831
TaqI	TCGA	6 cut(s)	8, 95, 747, 835, 969, 1101
TasI	AATT	9 cut(s)	43, 55, 64, 107, 127, 543, 808, 984, 989
TfiI	GAWTC	7 cut(s)	157, 215, 235, 634, 852, 963, 1095
Tru1I	TTAA	4 cut(s)	147, 639, 738, 1179
Tru9I	TTAA	4 cut(s)	147, 639, 738, 1179
TscAI	CASTG	1 cut(s)	1217
TseFI	GTSAC	4 cut(s)	824, 829, 1064, 1193
TseI	GCWGC	5 cut(s)	467, 470, 754, 869, 1073
Tsp45I	GTSAC	4 cut(s)	824, 829, 1064, 1193
TspDTI	ATGAA	6 cut(s)	386, 685, 774, 779, 1087, 1200
TspGWI	ACGGA	3 cut(s)	137, 562, 1212
TspRI	CASTG	1 cut(s)	1217
Vha464I	CTTAAG	1 cut(s)	638
XapI	RAATTY	3 cut(s)	64, 107, 127
XceI	RCATGY	1 cut(s)	1161
XhoI	CTCGAG	2 cut(s)	746, 968
XmnI	GAANNNNTTC	1 cut(s)	851
XspI	CTAG	3 cut(s)	342, 996, 1041
ZraI	GACGTC	1 cut(s)	102

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.